Elimination of Pathogen Biofilms via Postbiotics from Lactic Acid Bacteria: A Promising Method in Food and Biomedicine DOI Creative Commons

Jiahao Che,

Jingjing Shi,

Chenguang Fang

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(4), P. 704 - 704

Published: March 30, 2024

Pathogenic biofilms provide a naturally favorable barrier for microbial growth and are closely related to the virulence of pathogens. Postbiotics from lactic acid bacteria (LAB) secondary metabolites cellular components obtained by inactivation fermentation broth; they have certain inhibitory effect on all stages pathogen biofilms. LAB drawn attention because their high stability, safety dose parameters, long storage period, which give them broad application prospect in fields food medicine. The mechanisms eliminating via postbiotics mainly affect surface adhesion, self-aggregation, virulence, QS pathogens influencing interspecific intraspecific communication. However, there some factors (preparation process lack target) can limit antibiofilm impact postbiotics. Therefore, using delivery carrier optimizing interfering be eliminated. This review summarizes concept characteristics LAB, focusing preparation technology effect, applications limitations processing clinical treatment also discussed.

Language: Английский

Horizontal Gene Transfer of Antibiotic Resistance Genes in Biofilms DOI Creative Commons

Claudia Michaelis,

Elisabeth Grohmann

Antibiotics, Journal Year: 2023, Volume and Issue: 12(2), P. 328 - 328

Published: Feb. 4, 2023

Most bacteria attach to biotic or abiotic surfaces and are embedded in a complex matrix which is known as biofilm. Biofilm formation especially worrisome clinical settings it hinders the treatment of infections with antibiotics due facilitated acquisition antibiotic resistance genes (ARGs). Environmental now considered pivotal for driving biofilm formation, biofilm-mediated development dissemination. Several studies have demonstrated that environmental biofilms can be hotspots dissemination ARGs. These encoded on mobile genetic elements (MGEs) such conjugative mobilizable plasmids integrative (ICEs). ARGs rapidly transferred through horizontal gene transfer (HGT) has been shown occur more frequently than planktonic cultures. models promising tools mimic natural study via HGT. This review summarizes state-of-the-art techniques visualize three main HGT mechanisms biofilms: transformation, transduction, conjugation.

Language: Английский

Citations

223
Nanobiotics against antimicrobial resistance: harnessing the power of nanoscale materials and technologies DOI Creative Commons
Nayanika Chakraborty,

Diksha Jha,

Indrajit Roy

et al.

Journal of Nanobiotechnology, Journal Year: 2022, Volume and Issue: 20(1)

Published: Aug. 12, 2022

Abstract Given the spasmodic increment in antimicrobial resistance (AMR), world is on verge of “post-antibiotic era”. It anticipated that current SARS-CoV2 pandemic would worsen situation future, mainly due to lack new/next generation antimicrobials. In this context, nanoscale materials with potential have a great promise treat deadly pathogens. These functional are uniquely positioned effectively interfere bacterial systems and augment biofilm penetration. Most importantly, core substance, surface chemistry, shape, size nanomaterials define their efficacy while avoiding development AMR. Here, we review mechanisms AMR emerging applications as an excellent substitute for conventional antibiotics. We discuss potential, promises, challenges prospects nanobiotics combat Graphical

Language: Английский

Citations

96
Advances in Nanotechnology for Biofilm Inhibition DOI Creative Commons
Lokender Kumar, Monish Bisen, Kusum Harjai

et al.

ACS Omega, Journal Year: 2023, Volume and Issue: 8(24), P. 21391 - 21409

Published: June 7, 2023

Biofilm-associated infections have emerged as a significant public health challenge due to their persistent nature and increased resistance conventional treatment methods. The indiscriminate usage of antibiotics has made us susceptible range multidrug-resistant pathogens. These pathogens show reduced susceptibility intracellular survival. However, current methods for treating biofilms, such smart materials targeted drug delivery systems, not been found effective in preventing biofilm formation. To address this challenge, nanotechnology provided innovative solutions formation by clinically relevant Recent advances nanotechnological strategies, including metallic nanoparticles, functionalized dendrimers, polymeric cyclodextrin-based delivery, solid lipid polymer conjugates, liposomes, may provide valuable technological against infectious diseases. Therefore, it is imperative conduct comprehensive review summarize the recent advancements limitations advanced nanotechnologies. present Review encompasses summary agents, mechanisms that lead formation, impact on human health. In nutshell, offers survey managing infections. A detailed presentation how these strategies improve control prevent key objective mechanisms, applications, prospects nanotechnologies better understanding

Language: Английский

Citations

76
General Overview of Klebsiella pneumonia: Epidemiology and the Role of Siderophores in Its Pathogenicity DOI Creative Commons

Rim Abbas,

Mohamed Chakkour,

Hiba Zein El Dine

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(2), P. 78 - 78

Published: Jan. 27, 2024

The opportunistic pathogen Klebsiella pneumoniae (K. pneumoniae) can colonize mucosal surfaces and spread from mucosae to other tissues, causing fatal infections. Medical equipment the healthcare setting become colonized by species, which are widely distributed in nature be found water, soil, animals. Moreover, a substantial number of community-acquired illnesses also caused this organism worldwide. These infections characterized high rate morbidity mortality as well capacity metastatically. Hypervirulent strains thought connected these Four components critical bacterium’s pathogenicity—the capsule, lipopolysaccharide, fimbriae, siderophores. Siderophores secondary metabolites that allow iron sequester surrounding medium transport it intracellular compartment bacteria. A variables may lead K. colonization specific area. Risk factors for infection include local practices, antibiotic use misuse, control procedures, nutrition, gender, age.

Language: Английский

Citations

43
Relationship between biofilm formation and antibiotic resistance of Klebsiella pneumoniae and updates on antibiofilm therapeutic strategies DOI Creative Commons
Lifeng Li, Xueyan Gao, Mingchao Li

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Feb. 23, 2024

Klebsiella pneumoniae is a Gram-negative bacterium within the Enterobacteriaceae family that can cause multiple systemic infections, such as respiratory, blood, liver abscesses and urinary systems. Antibiotic resistance global health threat K. warrants special attention due to its most modern day antibiotics. Biofilm formation critical obstruction enhances antibiotic of . However, knowledge on molecular mechanisms biofilm relation with in limited. Understanding correlation crucial for providing insight design new drugs control treat biofilm-related infections. In this review, we summarize recent advances genes contributing , progress relationship between resistance, therapeutic strategies targeting biofilms. Finally, discuss future research directions target priority pathogen.

Language: Английский

Citations

16
Novel silver nanoparticle-based biomaterials for combating Klebsiella pneumoniae biofilms DOI Creative Commons

Eslam Elashkar,

Rihaf Alfaraj, Ola M. El‐Borady

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 9, 2025

Klebsiella pneumoniae is a significant nosocomial pathogen that has developed resistance to multiple antibiotics, often forming biofilms enhance its virulence. This study investigated the efficacy of novel nanoformulation, AgNPs@chitosan-NaF, in combating K. biofilms. Antimicrobial susceptibility testing was performed assess antibiotic profile isolates. The antibiofilm activity AgNPs@chitosan-NaF evaluated using crystal violet staining and scanning electron microscopy. underlying mechanisms action were through gene expression analysis. majority isolates exhibited high levels multidrug resistance. demonstrated superior biofilm inhibition compared AgNPs@chitosan, significantly reducing biomass disrupting architecture at MICs ranging from 0.125 1 μg/mL. Mechanistic studies revealed nanoformulation downregulated key biofilm-associated genes, including treC, fimA, mrkA, ecpA. While concentration-dependent cytotoxic effect on both normal cancer cell lines, minimal cytotoxicity observed concentrations below 31.25 highlights synergistic silver nanoparticles, chitosan, sodium fluoride emerges as promising therapeutic strategy address challenge multidrug-resistant bacterial infections.

Language: Английский

Citations

2
Comprehensive genomic insights into a highly pathogenic clone ST656 of mcr8.1 containing multidrug-resistant Klebsiella pneumoniae from Bangladesh DOI Creative Commons
Spencer Mark Mondol,

Mohammad Ali Hossain,

Fahim Kabir Monjurul Haque

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 18, 2025

Language: Английский

Citations

2
Antimicrobial Resistance: Two-Component Regulatory Systems and Multidrug Efflux Pumps DOI Creative Commons
Giuseppe Valerio De Gaetano, Germana Lentini, Agata Famà

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(6), P. 965 - 965

Published: May 26, 2023

The number of multidrug-resistant bacteria is rapidly spreading worldwide. Among the various mechanisms determining resistance to antimicrobial agents, multidrug efflux pumps play a noteworthy role because they export extraneous and noxious substrates from inside outside environment bacterial cell contributing (MDR) and, consequently, failure anti-infective therapies. expression can be under control transcriptional regulators two-component systems (TCS). TCS are major mechanism by which microorganisms sense reply external and/or intramembrane stimuli coordinating genes involved not only in pathogenic pathways but also antibiotic resistance. In this review, we describe influence on pump activity some Gram-negative Gram-positive bacteria. Taking into account strict correlation between pumps, development drugs targeting TCS, alone or together with already discovered inhibitors, may represent beneficial strategy contribute fight against growing

Language: Английский

Citations

40
Evolving biofilm inhibition and eradication in clinical settings through plant-based antibiofilm agents DOI Creative Commons
Eduarda Silva, J. A. Teixeira, Maria Olívia Pereira

et al.

Phytomedicine, Journal Year: 2023, Volume and Issue: 119, P. 154973 - 154973

Published: July 17, 2023

After almost 100 years since evidence of biofilm mode growth and decades intensive investigation about their formation, regulatory pathways mechanisms antimicrobial tolerance, nowadays there are still no therapeutic solutions to eradicate bacterial biofilms biomedical related issues.This review intends provide a comprehensive summary the recent most relevant published studies on plant-based products, or isolated compounds with antibiofilm activity action identified molecular targets against biofilms. The objective is offer new perspective data for clinical researchers aiming prevent eliminate biofilm-associated infections caused by pathogens.The search was performed considering original research articles PubMed, Web Science Scopus from 2015 April 2023, using keywords such as "antibiofilm", "antivirulence", "phytochemicals" "plant extracts".Over 180 were considered this focus priority human pathogens listed World Health Organization, including Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae Escherichia coli. Inhibition detachment dismantling formed these found extract/products derivative compounds. Although combination products antibiotics recorded discussed, topic currently poorly explored only reduced number species.This clearly demonstrates that may be promising strategy associated infections. thoroughly reviewing vast amount carried out over years, it concluded mostly able formation through inhibition quorum sensing signals, but also disrupt mature developed multidrug resistant bacteria targeting extracellular polymeric substance. Flavonoids phenolic seemed effective

Language: Английский

Citations

34
Antibiofilm properties of cathelicidin LL-37: an in-depth review DOI
Hamed Memariani, Mojtaba Memariani

World Journal of Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 39(4)

Published: Feb. 14, 2023

Language: Английский

Citations

26