Effect of continuous renal replacement therapy on the clinical efficacy and pharmacokinetics of polymyxin B in the treatment of severe pulmonary infection DOI Creative Commons
Xi Wang, Mingming Zhou, Xiyu Wang

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(6), P. e27558 - e27558

Published: March 1, 2024

ObjectiveThis study aimed to evaluate the pharmacokinetics of polymyxin B in patients with ventilator-associated pneumonia caused by multi-drug resistant bacteria, and analyze effect continuous renal replacement therapy (CRRT) on B.MethodsThirty-five bacteria admitted our hospital from June 2021 January 2022 were selected as subjects. The divided into standard group (n = 20) non-standard 15) based factors affecting compliance plasma concentration. received concentration was monitored. According monitoring results, they group, influencing blood Besides, then control 28) observation 7) according whether CRRT treatment. Patients treated alone, while CRRT. general data two groups compared. levels measured before next administration (Cmin), peak immediately after end infusion (Cmax) intermediate 6 h (midpoint dosing interval) (C1/2t) detected compared between groups. Correlation efficacy analyzed Spearman correlation. incidence complications 28-day mortality rate recorded.ResultsThe age, body mass index (BMI) Acute Physiology Chronic Health Evaluation II (APACHE II) scores higher than these (p < 0.05). BMI APACHE independent risk severe pulmonary infection There no significant differences BMI, APACHEII score, alanine aminotransferase level, aspartate albumin gender diabetes ratio > Cmin, Cmax, C1/2t lower 0.001). response 50.00% 36.36% correlation clinical 0.05), positive (r 0.485, p difference skin pigmentation, nephrotoxicity 0.05).ConclusionIn not receiving multidrug-resistant achieving drug usual recommended dose satisfactory. However, proportion a 6-h exceeding maximum high. important B. In undergoing CRRT, significantly reduced, suggesting that disease, played an role patient safety. may need be increased.

Language: Английский

Microbiota and Immunity during Respiratory Infections: Lung and Gut Affair DOI Open Access
Veronica Marrella,

Federico Nicchiotti,

Barbara Cassani

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 4051 - 4051

Published: April 5, 2024

Bacterial and viral respiratory tract infections are the most common infectious diseases, leading to worldwide morbidity mortality. In past 10 years, importance of lung microbiota emerged in context pulmonary although mechanisms by which it impacts intestinal environment have not yet been fully identified. On contrary, gut microbial dysbiosis is associated with disease etiology or/and development lung. this review, we present an overview microbiome modifications occurring during infections, namely, reduced community diversity increased burden, downstream consequences on host–pathogen interaction, inflammatory signals, cytokines production, turn affecting progression outcome. Particularly, focus role gut–lung bidirectional communication shaping inflammation immunity context, resuming both animal human studies. Moreover, discuss challenges possibilities related novel microbial-based (probiotics dietary supplementation) microbial-targeted therapies (antibacterial monoclonal antibodies bacteriophages), aimed remodel composition resident communities restore health. Finally, propose outlook some relevant questions field be answered future research, may translational relevance for prevention control infections.

Language: Английский

Citations

19
Metagenomic next generation sequencing of bronchoalveolar lavage fluids for the identification of pathogens in patients with pulmonary infection: A retrospective study DOI Creative Commons

Wenwen Qin,

Tai L. Guo, Tiebin You

et al.

Diagnostic Microbiology and Infectious Disease, Journal Year: 2024, Volume and Issue: 110(1), P. 116402 - 116402

Published: June 12, 2024

Due to the limitations of traditional laboratory methods (TMs), identification causative pathogens numerous pulmonary infections (PIs) remains difficult. This study evaluated value metagenomic next generation sequencing (mNGS) in various respiratory pathogens. A total 207 patients with TMs and mNGS data were collected for this retrospective study. included sputum culture, blood, bronchoalveolar lavage fluid (BALF) analysis, or polymerase chain reaction analysis throat swabs. Otherwise, BALF was analyzed using mNGS. For bacterial pathogens, sensitivities as compared 76.74 % 58.14 (P=0.012). fungal detection rate sensitivity higher that (93.68 vs 22.11 %; P<0.001). The positive predictive negative also greater Use offers good specificity thus facilitates clinical diagnosis PIs.

Language: Английский

Citations

4
Application of targeted next-generation sequencing for detecting respiratory pathogens in the sputum of patients with pulmonary infections DOI Creative Commons
Huijuan Da, Tze‐Chiang Meng, Yuanhong Xu

et al.

Infection Genetics and Evolution, Journal Year: 2025, Volume and Issue: 128, P. 105722 - 105722

Published: Feb. 4, 2025

Targeted next-generation sequencing (tNGS) might be valuable for identifying disease-causing pathogens. Herein, we assessed the utility of tNGS in diagnosing pulmonary infections using sputum samples. We gathered complete clinical information and tested specimens both conventional microbiological tests (CMTs) tNGS. The goal was to compare effectiveness these two methods detecting viral, bacterial, fungal Notably, demonstrated a higher pathogen detection rate compared CMTs (80.26 % [122/152] vs. 33.55 [51/152], P = 0.029). Specifically, more effective viruses than (90.00 28.07 %, 0.003). Moreover, detected certain fungi, such as Candida albicans Cryptococcus neoformans, although difference between assays not statistically significant (P > 0.05). Our findings reveal that offers advantages pathogens patients with lung infections, particularly bacteria viruses, providing complements CMTs.

Language: Английский

Citations

0
Deciphering the microbial landscape of lower respiratory tract infections: insights from metagenomics and machine learning DOI Creative Commons
Jiahuan Li,

Anying Xiong,

Junyi Wang

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: May 23, 2024

Background Lower respiratory tract infections represent prevalent ailments. Nonetheless, current comprehension of the microbial ecosystems within lower remains incomplete and necessitates further comprehensive assessment. Leveraging advancements in metagenomic next-generation sequencing (mNGS) technology alongside emergence machine learning, it is now viable to compare attributes communities among patients across diverse age groups, diseases, infection types. Method We collected bronchoalveolar lavage fluid samples from 138 diagnosed with conducted mNGS characterize lung microbiota. Employing various learning algorithms, we investigated correlation key bacteria concurrent bronchiectasis developed a predictive model for hospitalization duration based on these identified bacteria. Result observed variations different In elderly group, Pseudomonas aeruginosa exhibited highest relative abundance, followed by Corynebacterium striatum Acinetobacter baumannii . Methylobacterium Prevotella emerged as dominant genera at genus level younger while Mycobacterium tuberculosis Haemophilus influenzae were species. Within included , Klebsiella pneumoniae Significant differences presence phage JBD93 noted between group control group. concomitant fungal infections, most abundant predominant Notable Human gammaherpesvirus 4 betaherpesvirus 5 Candida albicans Aspergillus oryzae fumigatus bacterial Machine algorithms utilized select clinical indicators associated duration, confirming excellent performance predicting time. Conclusion Our study provided description characteristics offering insights perspectives. Additionally, advanced capability community features determining patients.

Language: Английский

Citations

3
Microbial signatures predictive of short-term prognosis in severe pneumonia DOI Creative Commons

Shen-Shen Huang,

Jiayong Qiu,

S Li

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Aug. 2, 2024

This retrospective cohort study aimed to investigate the composition and diversity of lung microbiota in patients with severe pneumonia explore its association short-term prognosis.

Language: Английский

Citations

2
Genomic characterization and molecular evolution of human monkeypox viruses DOI
Patrícia Aline Gröhs Ferrareze, Rute Alves Pereira e Costa, Claudia Elizabeth Thompson

et al.

Archives of Virology, Journal Year: 2023, Volume and Issue: 168(11)

Published: Oct. 21, 2023

Language: Английский

Citations

5
Effect of continuous renal replacement therapy on the clinical efficacy and pharmacokinetics of polymyxin B in the treatment of severe pulmonary infection DOI Creative Commons
Xi Wang, Mingming Zhou, Xiyu Wang

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(6), P. e27558 - e27558

Published: March 1, 2024

ObjectiveThis study aimed to evaluate the pharmacokinetics of polymyxin B in patients with ventilator-associated pneumonia caused by multi-drug resistant bacteria, and analyze effect continuous renal replacement therapy (CRRT) on B.MethodsThirty-five bacteria admitted our hospital from June 2021 January 2022 were selected as subjects. The divided into standard group (n = 20) non-standard 15) based factors affecting compliance plasma concentration. received concentration was monitored. According monitoring results, they group, influencing blood Besides, then control 28) observation 7) according whether CRRT treatment. Patients treated alone, while CRRT. general data two groups compared. levels measured before next administration (Cmin), peak immediately after end infusion (Cmax) intermediate 6 h (midpoint dosing interval) (C1/2t) detected compared between groups. Correlation efficacy analyzed Spearman correlation. incidence complications 28-day mortality rate recorded.ResultsThe age, body mass index (BMI) Acute Physiology Chronic Health Evaluation II (APACHE II) scores higher than these (p < 0.05). BMI APACHE independent risk severe pulmonary infection There no significant differences BMI, APACHEII score, alanine aminotransferase level, aspartate albumin gender diabetes ratio > Cmin, Cmax, C1/2t lower 0.001). response 50.00% 36.36% correlation clinical 0.05), positive (r 0.485, p difference skin pigmentation, nephrotoxicity 0.05).ConclusionIn not receiving multidrug-resistant achieving drug usual recommended dose satisfactory. However, proportion a 6-h exceeding maximum high. important B. In undergoing CRRT, significantly reduced, suggesting that disease, played an role patient safety. may need be increased.

Language: Английский

Citations

0