Research progress of autoimmune diseases based on induced pluripotent stem cells DOI Creative Commons

Rutong Ren,

Jinhe Jiang,

Xinxin Li

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: April 24, 2024

Autoimmune diseases can damage specific or multiple organs and tissues, influence the quality of life, even cause disability death. A 'disease in a dish' be developed based on patients-derived induced pluripotent stem cells (iPSCs) iPSCs-derived disease-relevant cell types to provide platform for pathogenesis research, phenotypical assays, therapy, drug discovery. With rapid progress molecular biology research methods including genome-sequencing technology, epigenetic analysis, '-omics' analysis organoid large amount data represents an opportunity help gaining in-depth understanding pathological mechanisms developing novel therapeutic strategies these diseases. This paper aimed review iPSCs-based phenotype confirmation, mechanism exploration, discovery, therapy autoimmune diseases, especially sclerosis, inflammatory bowel disease, type 1 diabetes using iPSCs cells.

Language: Английский

A short guide to the tight junction DOI Creative Commons
Sandra Citi, Michael Fromm, Mikio Furuse

et al.

Journal of Cell Science, Journal Year: 2024, Volume and Issue: 137(9)

Published: May 1, 2024

ABSTRACT Tight junctions (TJs) are specialized regions of contact between cells epithelial and endothelial tissues that form selective semipermeable paracellular barriers establish maintain body compartments with different fluid compositions. As such, the formation TJs represents a critical step in metazoan evolution, allowing multicompartmental organisms true, barrier-forming epithelia endothelia. In six decades have passed since first observations by transmission electron microscopy, much progress has been made understanding structure, function, molecular composition regulation TJs. The goal this Perspective is to highlight key concepts emerged through research future challenges lie ahead for field.

Language: Английский

Citations

27
Intestinal permeability and its significance in psychiatric disorders – A narrative review and future perspectives DOI
Jakub Wasiak, Oliwia Gawlik-Kotelnicka

Behavioural Brain Research, Journal Year: 2023, Volume and Issue: 448, P. 114459 - 114459

Published: April 28, 2023

Language: Английский

Citations

26
Claudin Barriers on the Brink: How Conflicting Tissue and Cellular Priorities Drive IBD Pathogenesis DOI Open Access

Christopher Capaldo

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(10), P. 8562 - 8562

Published: May 10, 2023

Inflammatory bowel diseases (IBDs) are characterized by acute or chronic recurring inflammation of the intestinal mucosa, often with increasing severity over time. Life-long morbidities and diminishing quality life for IBD patients compel a search better understanding molecular contributors to disease progression. One unifying feature IBDs is failure gut form an effective barrier, core role intercellular complexes called tight junctions. In this review, claudin family junction proteins discussed as they fundamental component barriers. Importantly, expression and/or protein localization altered in IBD, leading supposition that barrier dysfunction exacerbates immune hyperactivity disease. Claudins large transmembrane structural constrain passage ions, water, substances between cells. However, growing evidence suggests non-canonical functions during mucosal homeostasis healing after injury. Therefore, whether claudins participate adaptive pathological responses remains open question. By reviewing current studies, possibility assessed claudins, jack-of-all-trades master none. Potentially, robust wound restitution involve conflicting biophysical phenomena, exposing vulnerabilities tissue-wide frailty IBD.

Language: Английский

Citations

25
Health benefits of algae and marine-derived bioactive metabolites for modulating ulcerative colitis symptoms DOI
Najmeh Oliyaei, S. Zekri, Aida Iraji

et al.

Journal of Functional Foods, Journal Year: 2025, Volume and Issue: 125, P. 106690 - 106690

Published: Jan. 28, 2025

Language: Английский

Citations

1
Recent Advances in Cell‐Based In Vitro Models to Recreate Human Intestinal Inflammation DOI Creative Commons
Maria Helena Macedo, Mafalda D. Neto, Lorenzo Pastrana

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(31)

Published: Sept. 21, 2023

Abstract Inflammatory bowel disease causes a major burden to patients and healthcare systems, raising the need develop effective therapies. Technological advances in cell culture, allied with ethical issues, have propelled vitro models as essential tools study aetiology, its progression, possible Several cell‐based of intestinal inflammation been used, varying their complexity methodology induce inflammation. Immortalized lines are extensively used due long‐term survival, contrast primary cultures that short‐lived but patient‐specific. Recently, organoids organ‐chips demonstrated great potential by being physiologically more relevant. This review aims shed light on intricate nature cover recent works report human inflammation, encompassing diverse approaches outcomes.

Language: Английский

Citations

20
Neonatal enteroids absorb extracellular vesicles from human milk‐fed infant digestive fluid DOI Creative Commons
Claire K. Yung, Yang Zhang, Madeline Kuhn

et al.

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(4)

Published: April 1, 2024

Abstract Human milk contains extracellular vesicles (HMEVs). Pre‐clinical models suggest that HMEVs may enhance intestinal function and limit inflammation; however, it is unknown if or their cargo survive neonatal human digestion. This limits the ability to leverage HMEV as additives infant nutrition therapeutics. study aimed develop an EV isolation pipeline from small volumes of contents after feeding (digesta) address hypothesis in vivo digestion be taken up epithelial cells (IECs). Digesta was collected nasoduodenal sampling tubes ostomies. EVs were isolated raw pasteurized digesta by density‐gradient ultracentrifugation following two‐step skimming, acid precipitation caseins, multi‐step filtration. validated electron microscopy, western blotting, nanoparticle tracking analysis, resistive pulse sensing, super‐resolution microscopy. uptake tested enteroids. (dEVs) show typical morphology are enriched CD81 CD9, but depleted β‐casein lactalbumin. some dEV fractions contain mammary gland‐derived protein BTN1A1. Neonatal enteroids rapidly take dEVs part via clathrin‐mediated endocytosis. Our data can digestive fluid these absorbed IECs.

Language: Английский

Citations

7
Black soldier fly larvae oil (Hermetia illucens L.) calcium salt enhances intestinal morphology and barrier function in laying hens DOI Creative Commons
Muhsin Al Anas, Muhammad Anang Aprianto, Henny Akit

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(7), P. 103777 - 103777

Published: April 27, 2024

This study aimed to determine the influence of black soldier fly larvae oil calcium salt (BSFLO-SCa) supplementation on performance, jejunal histomorphology and gene expression tight junctions inflammatory cytokines in laying hens. A total 60 ISA Brown hens (40 wk age) were divided into 3 treatment groups, including a control group fed basal diet (T0) diets supplemented with 1% (T1) 2% (T2) BSFLO-SCa. Each consisted 5 replicates 4 each. Results showed that BSFLO-SCa significantly reduced (P < 0.05) feed conversion ratio (FCR), while egg weight (EW) increased 0.05). The inclusion both production (HDA) mass (EM). addition villus height (VH) width (VW), crypt depth (CD) junction claudin-1 (CLDN-1), junctional adhesion molecules-2 (JAM-2), occludin (OCLN) upregulated pro-inflammatory interleukin-6 (IL-6) was downregulated BSFLO-SCa, interleukin-18 (IL-18), toll-like receptor (TLR-4), tumor necrosis factor-α (TNF-α) On other hand, anti-inflammatory interleukin-13 (IL-13) interleukin-10 (IL-10) at In conclusion, dietary improved productivity, intestinal morphology integrity by upregulating junction-related protein addition, enhanced immune responses downregulating cytokine expression.

Language: Английский

Citations

5
Hyperglycemic environments directly compromise intestinal epithelial barrier function in an organoid model and hyaluronan (∼35kDa) protects via a layilin dependent mechanism DOI Creative Commons
Samreen Jatana,

Amina Abbadi,

Gail West

et al.

Matrix Biology, Journal Year: 2024, Volume and Issue: 133, P. 116 - 133

Published: Aug. 24, 2024

Metabolic syndrome and diabetes in obese individuals are strong risk factors for development of inflammatory bowel disease (IBD) colorectal cancer. The pathogenic mechanisms low-grade metabolic inflammation, including chronic hyperglycemic stress, disrupting gut homeostasis poorly understood. In this study, we sought to understand the impact a environment on intestinal barrier integrity protective effects small molecular weight (35 kDa) hyaluronan epithelial function.

Language: Английский

Citations

5
Intestinal Epithelial Tight Junction Barrier Regulation by Novel Pathways DOI
Arumugam Priya, Kushal Saha, Prashant K. Nighot

et al.

Inflammatory Bowel Diseases, Journal Year: 2024, Volume and Issue: 31(1), P. 259 - 271

Published: Sept. 25, 2024

Intestinal epithelial tight junctions (TJs), a dynamically regulated barrier structure composed of occludin and claudin family proteins, mediate the interaction between host external environment by allowing selective paracellular permeability luminal serosal compartments intestine. TJs are highly dynamic structures can undergo constant architectural remodeling in response to various stimuli. This is mediated an array intracellular signaling pathways that alters TJ protein expression localization. Dysfunctional regulation components compromising homeostasis important pathogenic factor for pathological conditions including inflammatory bowel disease (IBD). Previous studies have elucidated significance integrity key regulatory mechanisms through vitro vivo models. In recent years, considerable efforts been made understand crosstalk regulate formation disassembly TJs. review provides comprehensive view on novel permeability. We discuss latest evidence how ion transport, cytoskeleton extracellular matrix pathways, cell survival mechanism autophagy intestinal function. also provide perspective context-specific outcomes modulation. The knowledge diverse will further insights relevance defects potential target molecules/pathways IBD.

Language: Английский

Citations

5
The Unfolded Protein Response and Its Implications for Novel Therapeutic Strategies in Inflammatory Bowel Disease DOI Creative Commons

Noel Verjan Garcia,

Kyung U. Hong, Nobuyuki Matoba

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(7), P. 2066 - 2066

Published: July 23, 2023

The endoplasmic reticulum (ER) is a multifunctional organelle playing vital role in maintaining cell homeostasis, and disruptions to its functions can have detrimental effects on cells. Dysregulated ER stress the unfolded protein response (UPR) been linked various human diseases. For example, activation of UPR signaling pathways intestinal epithelial cells either exacerbate or alleviate severity inflammatory bowel disease (IBD), contingent degree conditions activation. Our recent studies shown that EPICERTIN, recombinant variant cholera toxin B subunit containing an retention motif, induce protective colon cells, subsequently promoting restitution mucosal healing IBD models. These findings support idea compounds modulating may be promising pharmaceutical candidates for treatment disease. In this review, we summarize our current understanding IBD, focusing their roles dysregulation, pathogenesis. Additionally, discuss therapeutic strategies promote cytoprotection reduce inflammation via pharmacological manipulation UPR.

Language: Английский

Citations

11