3D bioprinted cancer models: from basic biology to drug development

Nature reviews. Cancer, Journal Year: 2022, Volume and Issue: 22(12), P. 679 - 692

Published: Oct. 24, 2022

Language: Английский

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Advances in Spheroids and Organoids on a Chip

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(19)

Published: Feb. 22, 2023

Abstract Multicellular spheroids and organoids are promising in vitro 3D models personalized medicine drug screening. They replicate the structural functional characteristics of human organs vivo. Microfluidic technology micro‐nano fabrication can fulfill high requirement engineering approach growing research interest organoids. In this review, comparatively introduced. Then it is illustrated how spheroids‐ organoids‐on‐a‐chip facilitates their establishment, expansion, application through spatial‐temporal control, mechanical cues modeling, high‐throughput analysis, co‐culture, multi‐tissue interactions, biosensing, bioimaging integration. The potential opportunities challenges developing finally outlooked.

Language: Английский

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Nanoparticle-mediated thermal Cancer therapies: Strategies to improve clinical translatability

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 372, P. 751 - 777

Published: July 4, 2024

Despite significant advances, cancer remains a leading global cause of death. Current therapies often fail due to incomplete tumor removal and nonspecific targeting, spurring interest in alternative treatments. Hyperthermia, which uses elevated temperatures kill cells or boost their sensitivity radio/chemotherapy, has emerged as promising alternative. Recent advancements employ nanoparticles (NPs) heat mediators for selective cell destruction, minimizing damage healthy tissues. This approach, known NP hyperthermia, falls into two categories: photothermal (PTT) magnetothermal (MTT). PTT utilizes NPs that convert light heat, while MTT magnetic activated by alternating fields (AMF), both achieving localized damage. These methods offer advantages like precise minimal invasiveness, reduced systemic toxicity. However, the efficacy hyperthermia depends on many factors, particular, properties, microenvironment (TME), TME-NP interactions. Optimizing this treatment requires accurate monitoring strategies, such nanothermometry biologically relevant screening models can better mimic physiological features human body. review explores state-of-the-art NP-mediated discussing available nanomaterials, strengths weaknesses, characterization methods, future directions. Our particular focus lies preclinical techniques, providing an updated perspective relevance journey towards clinical trials.

Language: Английский

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Omics-based molecular classifications empowering in precision oncology

Cellular Oncology, Journal Year: 2024, Volume and Issue: 47(3), P. 759 - 777

Published: Jan. 31, 2024

Language: Английский

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Advances in 3D Bioprinting for Cancer Biology and Precision Medicine: From Matrix Design to Application

Advanced Healthcare Materials, Journal Year: 2022, Volume and Issue: 11(24)

Published: July 22, 2022

The tumor microenvironment is highly complex owing to its heterogeneous composition and dynamic nature. This makes tumors difficult replicate using traditional 2D cell culture models that are frequently used for studying biology drug screening. often leads poor translation of results between in vitro vivo reflected the extremely low success rates new candidate drugs delivered clinic. Therefore, there has been intense interest developing 3D laboratory representative patient samples. bioprinting an emerging technology enables biofabrication structures with virtue providing accurate control over distribution cells, biological molecules, matrix scaffolding. potential bridge gap by closely recapitulating microenvironment. Here, a brief overview provided key considerations discussed. Bioprinting techniques choice bioinks both natural synthetic polymers also outlined. Lastly, current bioprinted reviewed perspectives how clinical applications can greatly benefit from technologies offered.

Language: Английский

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Cancer-on-chip: a 3D model for the study of the tumor microenvironment

Journal of Biological Engineering, Journal Year: 2023, Volume and Issue: 17(1)

Published: Aug. 17, 2023

Abstract The approval of anticancer therapeutic strategies is still slowed down by the lack models able to faithfully reproduce in vivo cancer physiology. On one hand, conventional vitro fail recapitulate organ and tissue structures, fluid flows, mechanical stimuli characterizing human body compartments. other animal cannot typical tumor microenvironment, essential study behavior progression. This reviews cancer-on-chips as most promising tools model investigate microenvironment metastasis. We also described how cancer-on-chip devices have been developed implemented common primary cancers their metastatic sites. Pros cons this technology are then discussed highlighting future challenges close gap between pre-clinical clinical studies accelerate new therapies humans.

Language: Английский

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A co-culture system of macrophages with breast cancer tumoroids to study cell interactions and therapeutic responses

Cell Reports Methods, Journal Year: 2024, Volume and Issue: 4(6), P. 100792 - 100792

Published: June 1, 2024

3D tumoroids have revolutionized in vitro/ex vivo cancer biology by recapitulating the complex diversity of tumors. While provide new insights into development and treatment response, several limitations remain. As tumor microenvironment, especially immune system, strongly influences development, absence cells may lead to inappropriate conclusions. Macrophages, key players progression, are particularly challenging integrate tumoroids. In this study, we established three optimized standardized methods for co-culturing human macrophages with breast tumoroids: a semi-liquid model two matrix-embedded models tailored specific applications. We then tracked interactions macrophage infiltration these systems using flow cytometry light sheet microscopy showed that influenced not only tumoroid molecular profiles but also chemotherapy response. This underscores importance increasing complexity more accurately reflect conditions.

Language: Английский

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Stromal bone marrow fibroblasts and mesenchymal stem cells support acute myeloid leukaemia cells and promote therapy resistance

British Journal of Pharmacology, Journal Year: 2023, Volume and Issue: 181(2), P. 216 - 237

Published: Jan. 7, 2023

The bone marrow (BM) is the primary site of adult haematopoiesis, where stromal elements (e.g. fibroblasts and mesenchymal stem cells [MSCs]) work in concert to support blood cell development. However, establishment an abnormal clone can lead a malignancy, such as acute myeloid leukaemia (AML). Despite our increased understanding pathophysiology disease, patient survival remains suboptimal, mainly driven by development therapy resistance. In this review, we highlight importance MSCs health their impact on prognosis. We discuss how reduce killing effects therapies via combination contact‐dependent integrins) contact‐independent (i.e. secreted factors) mechanisms, accompanied immunosuppressive microenvironment. Importantly, underline challenges therapeutically targeting stroma improve outcomes, due inherent heterogeneity populations. LINKED ARTICLES This article part themed issue Cancer Microenvironment Pharmacological Interventions. To view other articles section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v181.2/issuetoc

Language: Английский

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A 3D In Vitro Triculture Hybrid Model Recapitulating Tumor Stromal Interaction of Triple‐Negative Breast Cancer as a High Throughput Anticancer Drug Screening Platform

Advanced Therapeutics, Journal Year: 2024, Volume and Issue: 7(5)

Published: Jan. 26, 2024

Abstract Breast cancer (BC) progression is substantially driven by cellular cross‐talk between tumor and stromal cells within the microenvironment (TME). However, lack of precise recapitulation heterocellular complexity, interactions in oversimplified 2D models, physiological differences animal models lead to discrepancy anticancer drug response. Three‐dimensional (3D) vitro bioengineered that physiologically resemble vivo TME are apt overcome preclinical testing outcome. Here, a compartmentalized 3D triculture triple‐negative breast (TC‐TNBC) model using silk‐fibroin (SF) scaffold GelMA hydrogel for recapitulating extracellular niche, respectively. The features heterogeneity niche incorporating most representative cell types tissue, i.e., adipocytes endothelial cells, MDA‐MB‐231 niche. TC‐TNBC exhibits enhanced tumorigenic potential presence cells. Screening drugs (doxorubicin (Dox) cisplatin (Cis)) an increase IC 50 concentration as compared monoculture, suggesting crucial role Drug sensitivity cytotoxicity behavior mimick like This provides facile adaptable platform represent tumor‐stromal high‐throughput screening.

Language: Английский

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Human Dermal Decellularized ECM Hydrogels as Scaffolds for 3D In Vitro Skin Aging Models

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 4020 - 4020

Published: April 4, 2024

Biomaterials play an important role in the development of advancing three dimensional (3D) vitro skin models, providing valuable insights for drug testing and tissue-specific modeling. Commercial materials, such as collagen, fibrin or alginate, have been widely used However, they do not adequately represent molecular complexity components. On this regard, novel biomaterials that tissues is becoming more design advanced models. In study, we obtained aged human decellularized dermal extracellular matrix (dECM) hydrogels extracted from cadaveric demonstrated their potential scaffold These dECM effectively reproduce complex fibrillar structure other common scaffolds, exhibiting similar mechanical properties, while preserving composition native dermis. It worth noting fibroblasts embedded within exhibit a behavior representative natural compared to commercial collagen hydrogels, where uncontrolled cell proliferation leads material shrinkage. The described hydrogel able be aging-on-a-chip model. results demonstrate preserve essential components dermis making them suitable option 3D aging models accurately cellular microenvironment, improving existing allowing reliable dermatopathological studies.

Language: Английский

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