Facultatively intra-bacterial localization of a planthopper endosymbiont as an adaptation to its vertical transmission DOI Creative Commons
Anna Michalik, Diego C. Franco, Teresa Szklarzewicz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 13, 2023

Abstract Transovarial transmission is the most reliable way of passing on essential nutrient- providing endosymbionts from mothers to offspring. However, not all endosymbiotic microbes follow complex path through female host tissues oocytes their own. Here we demonstrate an unusual strategy adapted by one planthopper Trypetimorpha occidentalis (Hemiptera: Tropiduchidae) Bulgaria. In this species, Acetobacteraceae endosymbiont transmitted transovarially within deep invaginations cellular membranes ancient Sulcia - strikingly resembling recently described plant virus transmission. in males, colonizes same bacteriocytes as but remains unenveloped. Then, endobacterial localization observed females appears be a unique adaptation maternal Further, symbiont’s genomic features, including encoding amino acid biosynthetic pathways and very similar psyllid symbiont, suggest combination ability horizontally transmit among species confer nutritional benefits. The close association with symbiont correlates so-far- unreported level erosion symbionts planthopper. , reflected substantial changes organization, reported for first time renown its stability. Vidania gene loss resulted smallest genomes known, at 109 kb. Thus, T. display adaptations features that expand our understanding how insect-microbe symbioses may evolve. Significance Statement Reliable across generations major challenge bacteria associate insects, independently established have addressed different ways. facultatively enveloped cells males Acetobacteraceae’s indicate evolutionary history, experienced demonstrates apparent consequences such association. Combined, multi-partite symbiosis expands diversity strategies insect form some consequences.

Language: Английский

Phylogeny and diversification of planthoppers (Hemiptera: Fulgoromorpha) based on a comprehensive molecular dataset and large taxon sampling DOI Creative Commons
Manon Bucher, Fabien L. Condamine, Yang Luo

et al.

Molecular Phylogenetics and Evolution, Journal Year: 2023, Volume and Issue: 186, P. 107862 - 107862

Published: June 16, 2023

Language: Английский

Citations

25
Genome Comparison Reveals Inversions and Alternative Evolutionary History of Nutritional Endosymbionts in Planthoppers (Hemiptera: Fulgoromorpha) DOI Creative Commons
Junchen Deng, Gordon M. Bennett, Diego C. Franco

et al.

Genome Biology and Evolution, Journal Year: 2023, Volume and Issue: 15(7)

Published: June 30, 2023

The evolutionary success of sap-feeding hemipteran insects in the suborder Auchenorrhyncha was enabled by nutritional contributions from their heritable endosymbiotic bacteria. However, symbiont diversity, functions, and origins this large insect group have not been broadly characterized using genomic tools. In particular, relationships among ancient betaproteobacterial symbionts Vidania (in Fulgoromorpha) Nasuia/Zinderia Cicadomorpha) are uncertain. Here, we genomes Sulcia three Pyrops planthoppers (family Fulgoridae) to understand metabolic functions histories. We find that, like previously planthoppers, these share responsibilities, with providing seven out ten essential amino acids. lineages across a highly conserved genome but multiple independent rearrangements occurring an early ancestor Cicadomorpha or Fulgoromorpha few succeeding lineages. Genomic synteny also observed within each genera Nasuia, Zinderia, Vidania, them, which challenges expectation shared ancestry for symbionts. further comparison other biological traits strongly suggests origin planthopper evolution possibly Nasuia Zinderia respective host This hypothesis links potential acquisition novel endosymbiont emergence auchenorrhynchan superfamilies.

Language: Английский

Citations

12
Colonization Resistance of Symbionts in Their Insect Hosts DOI Creative Commons
Zhengyan Wang,

Hanzi Yong,

Shan Zhang

et al.

Insects, Journal Year: 2023, Volume and Issue: 14(7), P. 594 - 594

Published: June 30, 2023

The symbiotic microbiome is critical in promoting insect resistance against colonization by exogenous microorganisms. mechanisms which symbionts contribute to the host’s immune capacity referred as resistance. Symbionts can protect insects from pathogens through a variety of mechanisms, including upregulating expression host immune-related genes, producing antimicrobial substances, and competitively excluding pathogens. Concordantly, have evolved fine-tuned regulatory avoid overactive responses or specialized cells harbor symbionts. Alternatively, some special adaptations, such formation biofilms increase their tolerance responses. Here, we provide review about hosts. Adaptations hosts that may maintain relationships, significance relationships coevolution systems are also discussed insights into in-depth study contribution physiology behavior.

Language: Английский

Citations

12
Diversity and genomics of bacteriome-associated symbionts in treehopper Darthula hardwickii (Hemiptera: Aetalionidae) and implications of their nutritional functions DOI Creative Commons
Han Xiao-hong,

Jinrui Zhou,

Qiong Guo

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

ABSTRACT Symbionts play important roles in insect nutritional ecology, and the phylogenies of some vertically transmitted symbionts mirror host phylogeny. Here we report diversity, distribution, transmission, potential functions harbored aetalionid treehopper Darthula hardwickii (Aetalionidae) using multiple methods compare its obligate symbiont Karelsulcia with that related Aetalion reticulatum. D. harbors bacteriomes, a yeast-like fungal (YLS) fat bodies, Tisiphia both bacteriomes bodies. YLS are to ovaries but do not cluster form “symbiont ball” terminal oocytes, as is case other auchenorrhynchan insects. represents first known instance being associated treehoppers. Phylogenetic analysis revealed Aetalionidae derived from within Membracidae. Gene truncation absence were tryptophan biosynthetic pathway , suggesting this no longer capable providing essential amino acid (EAA) host. Tryptophan presumed be supplied by since tryptophan-related genes either absent or degraded . No truncated found A. reticulatum it has lost synthesis EAAs, leafhoppers. This study sheds new light on diversity endosymbionts Membracoidea processes may have precipitated replacement diverse lineage. IMPORTANCE sap-feeding insects nutrition their hosts, which change through evolutionary time vary across lineages. comparative genomic indicates that, compared leaf- treehoppers, ability provide EAA function apparently performed coexisting (YLS). species treehopper, suggests involved treehoppers similar those observed sap-sucking analyses lineages largely phylogeny suggest originated Membracidae, contrast recent based data

Language: Английский

Citations

0
Flowing boundaries in autopoietic systems and microniche construction DOI

Matěj Jureček,

Jana Švorcová

Biosystems, Journal Year: 2025, Volume and Issue: unknown, P. 105477 - 105477

Published: May 1, 2025

Language: Английский

Citations

0
Limited variation in microbial communities across populations of Macrosteles leafhoppers (Hemiptera: Cicadellidae) DOI Creative Commons
Sandra Åhlén Mulio, Agnieszka Zwolińska, Tomasz Klejdysz

et al.

Environmental Microbiology Reports, Journal Year: 2024, Volume and Issue: 16(3)

Published: June 1, 2024

Microbial symbionts play crucial roles in insect biology, yet their diversity, distribution, and temporal dynamics across host populations remain poorly understood. In this study, we investigated the spatio-temporal distribution of bacterial within widely distributed economically significant leafhopper genus Macrosteles, with a focus on Macrosteles laevis. Using symbiont marker gene amplicon sequencing, explored intricate relationships between these insects microbial partners. Our analysis cytochrome oxidase subunit I (COI) data revealed several intriguing findings. First, there was no strong genetic differentiation M. laevis populations, suggesting flow among them. Second, observed levels heteroplasmy, indicating presence multiple mitochondrial haplotypes individuals. Third, parasitoid infections were prevalent, highlighting complex ecological interactions involving leafhoppers. The 16S rRNA confirmed universal ancient nutritional endosymbionts-Sulcia Nasuia-in Additionally, found high prevalence Arsenophonus, another common symbiont. Interestingly, unlike most previously studied species, exhibited only occasional cases infection known facultative endosymbionts other bacteria. Notably, variation different or sampling years same population. Comparatively, such as Rickettsia, Wolbachia, Cardinium Lariskella more species. These findings underscore importance considering both when studying associations. By simultaneously characterizing amplicons large collections, gain valuable insights into interplay Understanding contributes to our broader comprehension host-microbe natural ecosystems.

Language: Английский

Citations

3
A complete DNA repair system assembled by two endosymbionts restores heat tolerance of the insect host DOI Creative Commons
Xiaoyu Ling, Huijuan Guo,

Jian Di

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(51)

Published: Dec. 10, 2024

DNA repair systems are essential to maintain genome integrity and stability. Some obligate endosymbionts that experience long-term symbiosis with the insect hosts, however, have lost their key components for repair. It is largely unexplored how bacterial cope increased demand mismatch repairs under heat stresses. Here, we showed ibpA, a small shock protein encoded by

Language: Английский

Citations

3
Nutrient supplementation by genome-eroded Burkholderia symbionts of scale insects DOI Creative Commons
Anna Michalik, Eugen Bauer, Teresa Szklarzewicz

et al.

The ISME Journal, Journal Year: 2023, Volume and Issue: 17(12), P. 2221 - 2231

Published: Oct. 13, 2023

Abstract Hemipterans are known as hosts to bacterial or fungal symbionts that supplement their unbalanced diet with essential nutrients. Among them, scale insects (Coccomorpha) characterized by a particularly large diversity of symbiotic systems. Here, using microscopic and genomic approaches, we functionally the two belonging Eriococcidae family, Acanthococcus aceris Gossyparia spuria. These species host Burkholderia bacteria localized in cytoplasm fat body cells. Metagenome sequencing revealed very similar highly reduced genomes (<900KBp) low GC content (~38%), making them smallest most AT-biased yet sequenced. In eroded genomes, both retain biosynthetic pathways for amino acids leucine, isoleucine, valine, threonine, lysine, arginine, histidine, phenylalanine, precursors semi-essential acid tyrosine, well cobalamin-dependent methionine synthase MetH. A tryptophan biosynthesis pathway is conserved symbiont G. spuria, but appeared pseudogenized A. aceris, suggesting differential availability species’ diets. addition biosynthesis, maintain multiple cofactors, including riboflavin, cobalamin, thiamine, folate. The localization genome traits indicate symbiosis between eriococcids younger than other hemipteran symbioses, convergent. Our results add emerging picture dynamic replacements sap-sucking Hemiptera highlight widespread versatile intra- extracellular animals, plants, fungi.

Language: Английский

Citations

9
Obligate mutualistic heritable symbiosis in sap-feeding insects: an intricate relationship in nature DOI
María Eugenia Brentassi, Daniela de la Fuente

Symbiosis, Journal Year: 2024, Volume and Issue: 92(2), P. 159 - 185

Published: Jan. 22, 2024

Language: Английский

Citations

2
The nutritional dimension of facultative bacterial symbiosis in aphids: current status and methodological considerations for future research DOI Creative Commons
François Renoz

Current Research in Insect Science, Journal Year: 2023, Volume and Issue: 5, P. 100070 - 100070

Published: Dec. 20, 2023

Aphids are valuable models for studying the functional diversity of bacterial symbiosis in insects. In addition to their ancestral obligate nutritional symbiont

Language: Английский

Citations

6