Drug Target Insights,
Journal Year:
2025,
Volume and Issue:
19(1), P. 18 - 30
Published: April 2, 2025
Background:
Withania
somnifera
is
among
the
most
widely
prescribed
medicinal
plants
in
traditional
Indian
medicine.
Hydroalcoholic
extract
of
roots
this
plant
was
investigated
for
its
effects
on
overall
health
and
lifespan
model
worm
Caenorhabditis
elegans.
Methods:
The
extract’s
effect
fertility
observed
microscopically.
Worm
motility
quantified
through
an
automated
tracker.
metabolic
activity
worms
captured
using
Alamar
Blue®
assay.
Differential
gene
expression
extract-treated
revealed
a
whole
transcriptome
approach.
Results:
Extract-exposed
gnotobiotic
worms,
absence
any
bacterial
food,
registered
longer
lifespan,
higher
fertility,
better
motility,
activity.
Whole
analysis
differential
genes
associated
with
extension,
eggshell
assembly
integrity,
progeny
formation,
yolk
lipoproteins,
collagen
synthesis,
cuticle
molting,
etc.
This
seems
to
exert
beneficial
C.
elegans
partly
by
triggering
remodeling
developmentally
programmed
apical
extracellular
matrix
(aECM).
certain
important
(cpg-2,
cpg-3,
sqt-1,
dpy-4,
dpy-13,
col-17)
confirmed
PCR
assay
too.
Some
differently
expressed
(gfat-2,
unc-68,
col-109,
col-169,
rmd-1)
experiencing
pro-health
were
found
co-occurrence
have
their
homologous
counterpart
humans.
Conclusions:
Our
results
validate
suitability
W.
as
nutraceutical
healthy
aging.
Cancers,
Journal Year:
2023,
Volume and Issue:
15(7), P. 2059 - 2059
Published: March 30, 2023
Ovarian
cancer
(OC)
has
the
highest
mortality
rate
of
all
gynecological
malignancies
due
to
high
prevalence
advanced
stages
diagnosis
and
recurrence.
Furthermore,
heterogeneity
OC
tumors
contributes
rapid
development
resistance
conventional
chemotherapy.
In
recent
years,
in
order
overcome
these
problems,
targeted
therapies
have
been
introduced
various
types
tumors,
including
cancer.
However,
lack
predictive
biomarkers
showing
different
clinical
benefits
limits
effectiveness
therapies.
This
requires
preclinical
models
that
can
replicate
histological
molecular
characteristics
subtypes.
this
scenario,
organoids
become
an
important
model
for
personalized
medicine.
fact,
patient-derived
(PDO)
recapture
tumor
with
possibility
performing
drug
screening.
best
reproduce
patient's
characteristics,
it
is
necessary
develop
a
specific
extracellular
matrix
(ECM)
introduce
microenvironment
(TME),
which
both
represent
actual
object
study
improve
screening,
particularly
when
used
therapy
immunotherapy
guide
therapeutic
decisions.
review,
we
summarize
current
state
art
screening
PDOs,
ECM,
TME,
drugs
setting
OC,
as
well
discussing
implications
future
perspectives
research
organoids.
Chemical Engineering Journal,
Journal Year:
2024,
Volume and Issue:
485, P. 149849 - 149849
Published: Feb. 20, 2024
Aerogel
technology
is
an
emerging
platform
that
offers
alternative
and
cost-effective
dry
carriers
with
advanced
performances
for
the
pharmaceutical
industry.
The
innovative
combination
of
compressed
air-assisted
spray
gelation
''green''
supercritical
fluid
(SCF)
was
used
in
this
study
to
produce
alginate
aerogel
microparticles
adequate
properties
pulmonary
drug
delivery.
Beclomethasone
dipropionate
(BDP),
a
poorly
water-soluble
anti-inflammatory
asthma
treatment,
loaded
into
particles
by
SCF-assisted
impregnation
ensuring
high
loading,
as
well
amorphization
API.
production
aerogels
optimized
through
fine-tuning
parameters
gelation,
specifically
adjusting
air
flow
rate
pump
speed.
Alginate
were
aimed
be
produced
appropriate
aerodynamic
diameter
from
1
5
µm,
measured
vitro
deposition
tests
Next
Generation
impactor
potential
deep
lung
penetration.
Nuclear
magnetic
resonance
(NMR)
relaxometry
assess
hydration
unveiled
structure–property
relationships
leading
sudden
release
drug.
Finally,
cytotoxicity
fibroblasts
ex
vivo
permeability
conducted.
These
biological
confirmed
excellent
biocompatibility
formulations
demonstrated
efficient
BDP
porcine
bronchial
tissues
assisted
porous
carrier.
Cells,
Journal Year:
2023,
Volume and Issue:
12(2), P. 281 - 281
Published: Jan. 11, 2023
Additive
manufacturing
(AM)
or
industrial
3D
printing
uses
cutting-edge
technologies
and
materials
to
produce
a
variety
of
complex
products.
However,
the
effects
unintentionally
emitted
AM
(nano)particles
(AMPs)
on
human
cells
following
inhalation,
require
further
investigations.
The
physicochemical
characterization
AMPs,
extracted
from
filter
Laser
Powder
Bed
Fusion
(L-PBF)
printer
iron-based
materials,
disclosed
their
complexity,
in
terms
size,
shape,
chemistry.
Cell
Painting,
high-content
screening
(HCS)
assay,
was
used
detect
subtle
morphological
changes
elicited
by
AMPs
at
single
cell
resolution.
profiling
phenotypes,
prominent
concentration-dependent
cytoskeleton,
mitochondria,
membranous
structures
cell.
Furthermore,
lipidomics
confirmed
that
induced
extensive
membrane
remodeling
lung
epithelial
macrophage
co-culture
model.
To
elucidate
biological
mechanisms
action,
targeted
metabolomics
unveiled
several
inflammation-related
metabolites
regulating
response
AMP
exposure.
Overall,
exposure
led
internalization,
oxidative
stress,
cytoskeleton
disruption,
mitochondrial
activation,
remodeling,
metabolic
reprogramming
macrophages.
We
propose
approach
integrating
Painting
with
lipidomics,
as
an
advanced
nanosafety
methodology,
increasing
ability
capture
cellular
molecular
phenotypes
relevant
(nano)particle
Frontiers in Cell and Developmental Biology,
Journal Year:
2023,
Volume and Issue:
11
Published: June 30, 2023
Novel
minimally
invasive
strategies
are
needed
to
obtain
robust
bone
healing
in
complex
fractures
and
defects
the
elderly
population.
Local
cell
therapy
is
one
potential
option
for
future
treatment.
Mesenchymal
stromal
cells
(MSCs)
not
only
involved
osteogenesis
but
also
help
direct
recruitment
of
macrophages
during
regeneration
via
MSC-macrophage
crosstalk.
The
C-C
motif
chemokine
ligand
2
(CCL2)
an
inflammatory
that
associated
with
migration
MSCs
inflammation.
This
study
investigated
use
CCL2
as
a
therapeutic
target
local
therapy.
were
isolated
from
10
12
week-old
BALB/c
male
mice.
Genetically
modified
over-expressing
produced
using
murine
CCL2-secreting
pCDH-CMV-mCCL2-copGFP
expressing
lentivirus
vector.
Osteogenic
differentiation
assays
performed
or
without
co-culture.
Cell
performed.
transfected
vector
showed
higher
levels
secretion
compared
unaltered
(p
<
0.05).
Genetic
manipulation
did
affect
proliferation.
osteogenic
ability
alone.
However,
acute
(1
day)
sustained
(7
days)
stimulation
increased
alizarin
red-positive
area
when
co-cultured
0.001).
Both
recombinant
0.05)
released
facilitated
macrophage
migration.
We
demonstrated
promoted
subsequent
co-culture
macrophages.
Acute
potentially
facilitates
phase
by
directing
migration,
fostering
macrophage-MSC
crosstalk,
subsequently,
activating
licensing
pro-inflammatory
cytokines.
combination
CCL2,
MSCs,
could
be
strategy
compromised
healing.
Journal of Functional Biomaterials,
Journal Year:
2024,
Volume and Issue:
15(3), P. 77 - 77
Published: March 20, 2024
Gelatin
methacrylate
(GelMA)
is
a
photocrosslinkable
biomaterial
that
has
gained
widespread
use
in
tissue
engineering
due
to
its
favorable
biological
attributes
and
customizable
physical
mechanical
traits.
While
GelMA
compatible
with
various
cell
types,
distinct
cellular
responses
are
observed
within
hydrogels.
As
such,
tailoring
hydrogels
for
specific
applications
become
imperative.
Thus,
our
objective
was
develop
tailored
enhance
viability
specifically
TC28a2
chondrocytes
three-dimensional
(3D)
culture
setting.
We
investigated
synthesis
using
PBS
0.25M
CB
buffer,
analyzed
the
traits
of
hydrogels,
evaluated
how
varying
crosslinking
conditions
(GelMA
concentration,
photoinitiator
UV
exposure
time)
affected
chondrocytes.
The
results
revealed
buffer
led
greater
degree
methacrylation
compared
LAP
demonstrated
superior
efficacy
gelation
Irgacure
2959.
Additionally,
stiffness,
porosity,
swelling
were
predominantly
by
while
impacted
all
conditions,
decreasing
notably
increasing
time.
This
study
facilitated
optimization
critical
aspect
diverse
biomedical
applications.
