Biofilm Lifestyle in Recurrent Urinary Tract Infections

Life, Journal Year: 2023, Volume and Issue: 13(1), P. 148 - 148

Published: Jan. 4, 2023

Urinary tract infections (UTIs) represent one of the most common that are frequently encountered in health care facilities. One main mechanisms used by bacteria allows them to survive hostile environments is biofilm formation. Biofilms closed bacterial communities offer protection and safe hiding, allowing evade host defenses hide from reach antibiotics. Inside communities, show an increased rate horizontal gene transfer exchange resistance virulence genes. Additionally, communication within orchestrate expression genes, which further cements infestation increases invasiveness infection. These facts stress necessity continuously updating our information understanding etiology, pathogenesis, eradication methods this growing public concern. This review seeks understand role formation recurrent urinary tact outlining underlying different uropathogens, addition shedding light on some strategies.

Language: Английский

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Anti-Quorum Sensing Activities of Gliptins against Pseudomonas aeruginosa and Staphylococcus aureus

Biomedicines, Journal Year: 2022, Volume and Issue: 10(5), P. 1169 - 1169

Published: May 18, 2022

The development of bacterial resistance to traditional antibiotics constitutes an emerging public health issue. Promising approaches have been innovated conquer resistance, and targeting virulence is one these approaches. Bacterial mitigation offers several merits, as antivirulence agents do not affect the growth bacteria hence induce develop resistance. In this direction, numerous drugs repurposed prior their clinical use alone or in combination with antibiotics. Quorum sensing (QS) plays a key role controlling virulence. current study, dipeptidase inhibitor-4 (DPI-4) antidiabetic gliptins were screened for anti-quorum (anti-QS) activities against Gram-negative Pseudomonas aeruginosa Gram-positive Staphylococcus aureus. Upon assessing antibiofilm activities, ten tested significantly diminished biofilm formation. particular, sitagliptin exhibited most efficient activity, so it was chosen representative all further investigate its activity. Sitagliptin protected mice from P. S. aureus pathogenesis. Furthermore, downregulated QS-encoding genes To test anti-QS gliptins, detailed molecular docking study conducted evaluate gliptins' binding affinities QS receptors, which helped explain particularly omarigliptin. conclusion, evaluates possible that could be promising novel candidates treatment aggressive -positive infections either adjuvants other

Language: Английский

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Characterization of the Anti-Biofilm and Anti-Quorum Sensing Activities of the β-Adrenoreceptor Antagonist Atenolol against Gram-Negative Bacterial Pathogens

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(21), P. 13088 - 13088

Published: Oct. 28, 2022

The development of bacterial resistance to antibiotics is an increasing public health issue that worsens with the formation biofilms. Quorum sensing (QS) orchestrates virulence and controls biofilm. Targeting promising approach overcome increment antibiotics. In a previous detailed in silico study, anti-QS activities twenty-two β-adrenoreceptor blockers were screened supposing atenolol as candidate. current study aims evaluate anti-QS, anti-biofilm anti-virulence blocker against Gram-negative bacteria Serratia marcescens, Pseudomonas aeruginosa, Proteus mirabilis. An was conducted binding affinity S. marcescens SmaR QS receptor, P. aeruginosa QscR mirabilis MrpH adhesin. activity evaluated tested strains vitro vivo. present finding shows considerable ability compete proteins significantly downregulated expression QS- virulence-encoding genes. Atenolol showed significant reduction biofilm formation, enzyme production, motility. Furthermore, diminished capacity for killing protected mice. conclusion, has potential can be used adjuvant treatment aggressive infections.

Language: Английский

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Diminishing the Pathogenesis of the Food-Borne Pathogen Serratia marcescens by Low Doses of Sodium Citrate

Biology, Journal Year: 2023, Volume and Issue: 12(4), P. 504 - 504

Published: March 26, 2023

Protecting food from bacterial contamination is crucial for ensuring its safety and avoiding foodborne illness. Serratia marcescens one of the contaminants that can form biofilms pigments spoil product could cause infections illness to consumer. Food preservation essential diminish such or at least reduce their pathogenesis; however, it should not affect odor, taste, consistency must be safe. Sodium citrate a well-known safe additive current study aims evaluate anti-virulence anti-biofilm activity low concentrations against S. marcescens. The antibiofilm activities sodium were evaluated phenotypically genotypically. results showed significant effect on decreasing biofilm formation other virulence factors, as motility production prodigiosin, protease, hemolysins. This owed downregulating virulence-encoding genes. An in vivo investigation was conducted mice histopathological examination isolated tissues liver kidney confirmed citrate. In addition, an silico docking binding ability quorum sensing (QS) receptors regulates virulence. marked virtual compete QS proteins, which explain citrate's effect. conclusion, used prevent by bacteria.

Language: Английский

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Drug repositioning: doxazosin attenuates the virulence factors and biofilm formation in Gram-negative bacteria

Applied Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 107(11), P. 3763 - 3778

Published: April 20, 2023

Language: Английский

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What’s old is new again: Insights into diabetic foot microbiome

World Journal of Diabetes, Journal Year: 2023, Volume and Issue: 14(6), P. 680 - 704

Published: June 14, 2023

Diabetes is a chronic disease that considered one of the most stubborn global health problems continues to defy efforts scientists and physicians. The prevalence diabetes in population grow alarming levels year after year, causing an increase incidence complications care costs all over world. One major complication high susceptibility infections especially lower limbs due immunocompromised state diabetic patients, which definitive factor cases. Diabetic foot continue be common patients are associated with risk serious such as bone infection, limb amputations, life-threatening systemic infections. In this review, we discussed circumstances infection well some commonly isolated pathogens from related virulence behavior. addition, shed light on different treatment strategies aim at eradicating infection.

Language: Английский

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Synergistic Benefits: Exploring the Anti-Virulence Effects of Metformin/Vildagliptin Antidiabetic Combination against Pseudomonas aeruginosa via Controlling Quorum Sensing Systems

Biomedicines, Journal Year: 2023, Volume and Issue: 11(5), P. 1442 - 1442

Published: May 14, 2023

The repurposing of drugs is one the most competent strategies for discovering new antimicrobial agents. Vildagliptin a dipeptidyl peptidase-4 inhibitor (DPI-4) that used effectively in combination with metformin to control blood glucose levels diabetic patients. This study was designed evaluate anti-virulence activities this against clinically important pathogens, Pseudomonas aeruginosa. current findings show significant ability vildagliptin-metformin diminish biofilm formation, bacterial motility, and production virulent extracellular enzymes pyocyanin pigment. Furthermore, drug significantly increased susceptibility P. aeruginosa oxidative stress, indicating immunity enhancement eradication cells. In compliance vitro findings, histopathological photomicrographs mice showed considerable protective effect metformin-vildagliptin aeruginosa, revealing relief inflammation due aeruginosa-induced pathogenesis. mainly employs quorum sensing (QS) systems its huge arsenal virulence factors. can be interrupted by anti-QS both vildagliptin, as exhibited affinity QS receptors. Additionally, downregulated expression main three QS-encoding genes These at very low concentrations (10, 1.25 mg/mL, respectively) compared (850, 50 diabetes.

Language: Английский

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Assessing the antibacterial potential of 6-gingerol: Combined experimental and computational approaches

Saudi Pharmaceutical Journal, Journal Year: 2024, Volume and Issue: 32(5), P. 102041 - 102041

Published: March 18, 2024

The rise of antibiotic resistance in bacteria is becoming a global concern, particularly due to the dwindling supply new antibiotics. This situation mandates discovery antimicrobial candidates. Plant-derived natural compounds have historically played crucial role development antibiotics, serving as rich source substances possessing properties. Numerous studies supported reputation 6-gingerol, prominent compound found ginger family, for its antibacterial In this study, activities 6-gingerol were evaluated against Gram-negative bacteria, Acinetobacter baumannii and Klebsiella pneumoniae, with particular focus on clinically significant Pseudomonas aeruginosa Gram-positive Staphylococcus aureus. Furthermore, anti-virulence assessed vitro, vivo, silico. current findings showed that 6-gingerol's activity effect disruption bacterial cell membrane efflux pumps, it significantly decreased disrupted S. aureus P. aeruginosa. biofilm formation production virulence factors concentrations below MICs. properties could be attributed capacity disrupt virulence-regulating systems; quorum sensing (QS). 6-Gingerol was interact QS receptors downregulate genes responsible QS. addition, molecular docking, dynamics (MD) simulation results indicated comparable binding affinity co-crystalized ligands different targets well stable interactions during 100 ns MD simulations. These suggest holds promise an agent can combined antibiotics treatment severe infections.

Language: Английский

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Utilization of zein nano-based system for promoting antibiofilm and anti-virulence activities of curcumin against Pseudomonas aeruginosa

Nanotechnology Reviews, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 1, 2024

Abstract Bacterial biofilms contribute to increased pathogenesis and bacterial resistance. Biofilms can enhance pathogenicity by shielding bacteria from the immune system antibiotics, they are associated with persistent infections. Additionally, antibiotic resistance mechanisms within make them challenging treat, emphasizing need for strategies be addressed. Mitigating virulence is a promising strategy that could ease their eradication host immunity without stressing induce The merits of this augmented when using safe anti-virulence candidates in proper formulations. current study aimed evaluate antibiofilm efficacy curcumin–zein nanoparticles against Pseudomonas aeruginosa . In vitro investigations were performed assess effect on biofilm formation, motility, production factors, including proteases, hemolysins, pyocyanin, comparison bulk curcumin. Furthermore, expression genes encode quorum sensing (QS) systems regulate was assessed. An silico done affinity curcumin QS receptors. an vivo protection assay inhibitory our preparation diminishing P. aeruginosa’s capacity pathogenesis. results showed significant activities compared These attributed curcumin’s interfering its virulence. conclusion, acquires anti-QS, anti-virulence, vastly enhanced upon loading zein nanoparticles.

Language: Английский

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Unveiling the hidden language of bacteria: anti-quorum sensing strategies for gram-negative bacteria infection control

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(3)

Published: Feb. 27, 2024

Language: Английский

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