Determining Antioxidant Activity of Cannabis Leaves Extracts from Different Varieties—Unveiling Nature’s Treasure Trove

Antioxidants, Journal Year: 2023, Volume and Issue: 12(7), P. 1390 - 1390

Published: July 6, 2023

Cannabis leaves contain a diverse range of antioxidants, including cannabinoids, flavonoids, and phenolic compounds, which offer significant health benefits. Utilising cannabis as source antioxidants presents cost-effective approach because they are typically discarded during the cultivation plants for their seeds or fibres. Therefore, this presented study aimed to assess antioxidant activity selected hemp cultivars, such Białobrzeska, Tygra, Henola, based on results obtained with 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid, ferric reducing power, cupric capacity, 2,2-Diphenyl-1-picrylhydrazyl assays. The cannabinoid profile was analysed contents cannabidiol (CBD), cannabigerol (CBG), Δ9-tetrahydrocannabinol (Δ9-THC), cannabichromene (CBC), determined chromatographic following variables were tested: impact various extractants (methanol, ethanol, isopropanol), mixtures (50:50, v/v, well extraction methods (maceration ultra-sound-assisted extraction) in obtaining extracts characterised by different profiles. revealed that selection extractant conditions significantly influenced active compounds' efficiency activity. Among tested conditions, ultrasound-assisted using methanol yielded highest profile: CBD = 184.51 ± 5.61; CBG 6.10 0.21; Δ9-THC 0.51 0.01; CBC 0.71 0.01 μg/g potential Białobrzeska leaf extracts.

Language: Английский

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Opportunities and challenges in waste management within the medicinal cannabis sector

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 197, P. 116639 - 116639

Published: March 28, 2023

Language: Английский

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Industrial Hemp As a Multi-Purpose Crop: Last Achievements and Research in 2018−2023

Journal of Natural Fibers, Journal Year: 2024, Volume and Issue: 21(1)

Published: June 27, 2024

Industrial hemp cultivation was restricted years ago in most countries, thus wiping out centuries of science and genetic resources. It is only since the 1990s that has been experiencing its renaissance, which nowadays driven by new strategies. The objective review to compile summarize latest research achievements. focus on from 2018 2023. VOSviewer software used define main areas trends. Most articles were discussed, among others determination average yield loss due water stress (8.7 Mg ha−1) low temperatures (0.4 ha−1); increase up 25% achieving rejecting smallest seeds; or definition optimum dose sewage sludge application (25 ha−1). Regarding composition properties: identification caflanone flavonoid (selective activity against certain neoplasms); 71 compounds (monoterpenes, sesquiterpenes CBs) EO; antioxidant capacity EO (63 mg TE g−1 438 g−1); comparison content monoicous, female male inflorescences (0.10%, 0.15% 0.07% respectively). current possibilities using, e.g. for phytoremediation bioenergy, presented. Also knowledge gaps future identified.

Language: Английский

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Supercritical fluid extraction of cannabinoids and their analysis by liquid chromatography and supercritical fluid chromatography: A short review

Journal of CO2 Utilization, Journal Year: 2024, Volume and Issue: 86, P. 102907 - 102907

Published: Aug. 1, 2024

Extraction of cannabinoids from different parts the plant matrix is often carried out by various traditional methods based on use organic solvents. Supercritical fluid extraction (SFE) has emerged as one most intriguing approaches for cannabinoids. This review examines importance SFE cannabinoids, parameters, selection a suitable co-solvent/modifier, and appropriate sample preparation. To characterise composition mixture analysis can be applied. One them high-performance liquid chromatography (HPLC), which requires no derivatisation analysed allows determination wide variety both acidic neutral Nevertheless, with tandem mass spectrometry (LC-MS/MS) gaining increasing due to its superior identification analytes retention times well specific qualifier ions. Another interesting analytical method supercritical (SFC), uses fluids (SCFs) such CO2 in combination modifiers, successfully separate determine The SFC an efficient above all, rapid separation desired analytes. There essential need efficiently investigate influence experimental parameters behaviour SFC.

Language: Английский

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Extraction, Characterization, and Chitosan Microencapsulation of Bioactive Compounds from Cannabis sativa L., Cannabis indica L., and Mitragyna speiosa K.

Antioxidants, Journal Year: 2022, Volume and Issue: 11(11), P. 2103 - 2103

Published: Oct. 25, 2022

The objective of the research was to investigate bioactive compounds herbal plant leaves by microencapsulation technique for future application as a feed additive. In this experiment, three leaves, namely Cannabis sativa L., indica and Mitragyna speiosa K., were comparatively investigated using different methods extract their compounds. Two used compounds: microwave extraction (water-heating transferred) maceration (methanol extracted). results obtained revealed that total polyphenolic flavonoid contents antioxidant capacity significantly higher stronger, respectively, than those produced method (p < 0.05). Furthermore, spray-drying employed enhance extracted encapsulation with chitosan through ionic gelation properties. physical characteristics chitosan-encapsulated substrates examined under scanning electron microscope (SEM) microparticle size (1.45 11.0 µm). efficiency found be 99.7, 82.3, 54.6% microencapsulated M. speiosa, C. indica, sativa, respectively. Therefore, treatment prior leaf extracts resulted in increased recovery compound encroachment.

Language: Английский

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Phenolic profiles of non‐industrial hemp (Cannabis sativa L.) seed varieties collected from four different Moroccan regions

International Journal of Food Science & Technology, Journal Year: 2023, Volume and Issue: 58(3), P. 1367 - 1381

Published: Jan. 9, 2023

Summary This study aims to characterise the seeds of two varieties (Beldia and Critical) hemp grown in four geographical areas northern Morocco regarding their phenolic compounds antioxidant power. Thirty‐three were identified using HPLC‐DAD/ESI‐MS 2 technique. The profiles all from regions characterised by richness N‐trans ‐caffeoyltyramine (390.22–721.41 μg g −1 ), cannabisin A (217.96–393.37 CTE ) B (195.25–331.28 ). activities (expressed as IC 50 EC values) 1.83–4.14, 1.64–4.37, 2.45–6.02, 2.65–9.29 1.75–4.37 mg mL extract for TAC, DPPH, ABTS, CUPRAC FRAP tests respectively. two‐way analysis variance showed that compounds' content was mainly related location its interaction with genotype factor. Multivariate Jebha Galaz a high level potent activity compared Tamorot Ratba regions. characterisation constitutes an interesting database breeders create new meet fluctuating expectations cannabis industry.

Language: Английский

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Plant-based natural product chemistry: An overview of the multistep journey involved in scientific validation of traditional knowledge

Studies in natural products chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 327 - 377

Published: Jan. 1, 2024

Language: Английский

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The Use of Compounds Derived from Cannabis sativa in the Treatment of Epilepsy, Painful Conditions, and Neuropsychiatric and Neurodegenerative Disorders

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5749 - 5749

Published: May 25, 2024

Neurological disorders present a wide range of symptoms and challenges in diagnosis treatment. Cannabis sativa, with its diverse chemical composition, offers potential therapeutic benefits due to anticonvulsive, analgesic, anti-inflammatory, neuroprotective properties. Beyond cannabinoids, cannabis contains terpenes polyphenols, which synergistically enhance pharmacological effects. Various administration routes, including vaporization, oral ingestion, sublingual, rectal, provide flexibility treatment delivery. This review shows the efficacy managing neurological such as epilepsy, neurodegenerative diseases, neurodevelopmental disorders, psychiatric painful pathologies. Drawing from surveys, patient studies, clinical trials, it highlights alleviating symptoms, slowing disease progression, improving overall quality life for patients. Understanding mechanisms can open up possibilities using this plant individual needs.

Language: Английский

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Ultrasound-Assisted Extraction of Total Phenolic Compounds and Antioxidant Activity in Mushrooms

Agronomy, Journal Year: 2022, Volume and Issue: 12(8), P. 1812 - 1812

Published: July 30, 2022

The consumption of mushrooms has considerably increased in recent years because their beneficial nutritional properties due to essential amino acids, proteins, and dietary fiber content. Recent research shown that they are also rich polysaccharides phenolic compounds. These compounds exhibit decisive free radical ROS scavenging power with potential application the treatment neurodegenerative disorders. In addition, present important like antioxidant, antiaging, immune modulation. research, optimization for extraction total antioxidant activity (DPPH ABTS), based on ultrasound–assisted techniques been carried out. Five variables (% MeOH solvent, temperature, amplitude, cycle, sample:solvent ratio have selected; both content as well ABTS)) considered response variables. optimal conditions, determined by means a multiresponse method, were established at 0.2 g sample extracted 15.3 mL solvent (93.6% MeOH) 60 °C 5 min using 16.86% amplitude 0.71 s−1 cycles. A precision study optimized method performed deviations lower than 5%, which proves repeatability method. Finally, applied wild commercial from Andalusia Northern Morocco, confirmed its suitability mushroom samples, while ensuring maximum activity.

Language: Английский

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Phytochemical Analysis of the Methanolic Extract and Essential Oil from Leaves of Industrial Hemp Futura 75 Cultivar: Isolation of a New Cannabinoid Derivative and Biological Profile Using Computational Approaches

Plants, Journal Year: 2022, Volume and Issue: 11(13), P. 1671 - 1671

Published: June 24, 2022

Cannabis sativa L. is a plant belonging to the Cannabaceae family, cultivated for its psychoactive cannabinoid (Δ9-THC) concentration or fiber and nutrient content in industrial use. Industrial hemp shows low Δ9-THC level valuable source of phytochemicals, mainly represented by cannabinoids, flavones, terpenes, alkaloids, with health-promoting effects. In present study, we investigated phytochemical composition leaves cultivar Futura 75, monoecious commercially used food preparations cosmetic purposes. Leaves are generally discarded, represent waste products. We analyzed methanol extract 75 HPLC NMR spectroscopy essential oil GC-MS. addition, order compare chemical constituents, prepared water infusion. One new derivative (1) seven known components, namely, cannabidiol (2), cannabidiolic acid (3), β-cannabispirol (4), (5), canniprene (6), cannabiripsol (7), cannflavin B (8) were identified. The CBD was highest all preparations. outcomes computational study focused on elucidating role 2α-hydroxy-Δ3,7-cannabitriol (1), CBDA (3) inflammation thrombogenesis.

Language: Английский

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