Antioxidant components of ram seminal plasma are influenced by age, season, and the RsaI polymorphism of the MTNR1A gene

Animal Reproduction Science, Journal Year: 2025, Volume and Issue: unknown, P. 107853 - 107853

Published: May 1, 2025

Language: Английский

---

Effects of oral black Maca ( Lepidium meyenii ) supplementation on semen quality and refrigerated storage stability in subfertile and normofertile dogs: a study on sperm parameters and testosterone levels

Veterinary Quarterly, Journal Year: 2025, Volume and Issue: 45(1), P. 10 - 22

Published: May 2, 2025

The aim of this study was to assess the beneficial effects oral supplementation Lepidium meyenii (Maca) on improving and keeping sperm quality in dogs during storage, investigate its effect changes testosterone concentrations. Forty male were enrolled divided into four groups ten each: two subfertile (control treatment) normofertile groups. treatment received Maca a capsule formulation (75 mg/kg), while control placebo. spermiogram levels assessed at three times cycle: 0 (T0), 31 (T31), 62 (T62) days. Ejaculates processed for storage 5 °C evaluated total progressive motility membrane integrity 3 (T3h), 24 (T24h), 48 (T48h), 72 h (T72h) post storage. 75 mg/kg extract can improve parameters increase serum concentrations, leading improved reproductive capacity. semen subjects treated with maintained stable longer period when stored compared subjects, demonstrating use fertility.

Language: Английский

---

New Insight on the In Vitro Effects of Melatonin in Preserving Human Sperm Quality

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(9), P. 5128 - 5128

Published: May 4, 2022

Spermatozoa (SPZ) are sensitive to stressful conditions, particularly oxidative stress, which alters their quality; thus, the use of protective molecules as an antioxidant is encouraged. Herein, we used melatonin (MLT) investigate its in vitro effects on human sperm parameters under conditions stress induced by cadmium (Cd). Fifteen semen samples were divided into control, Cd-treated, MLT-treated, and Cd+MLT-treated groups analyzed after 30 min, 6 h, 24 h exposure. Results showed a time-dependent decrease SPZ motility, DNA integrity, increased apoptosis these counteracted MLT co-treatment. Based data, further explored additional just at h. The highlighted lipid peroxidation, reduced percentage able undertake acrosome reaction altered levels localization some protein markers motility (PREP, RSPH6A), morphology (DAAM1), membrane (PTMA, IAM38); all Interestingly, alone was ameliorate min incubation compared while it prevented physiological alteration terms apoptosis. Collectively, data encourage integrative molecule gamete quality when compromised conditions.

Language: Английский

---

Seminal Plasma Antioxidants Are Related to Sperm Cryotolerance in the Horse

Antioxidants, Journal Year: 2022, Volume and Issue: 11(7), P. 1279 - 1279

Published: June 28, 2022

The objective of this study was to determine the relationship enzymatic (superoxide dismutase, SOD; glutathione peroxidase, GPX; catalase, CAT; and paraoxonase type 1, PON1) non-enzymatic antioxidants (measured in terms of: Trolox equivalent antioxidant capacity, TEAC; cupric-reducing CUPRAC; ferric-reducing ability plasma, FRAP), as well oxidative stress index (OSI) seminal plasma (SP) with resilience horse sperm freeze-thawing. Twenty-one ejaculates (one per individual) were collected split into two aliquots: first used harvest SP assess activity levels OSI, second one cryopreserved. following post-thaw quality parameters evaluated: motility, membrane acrosome integrity, mitochondrial potential, intracellular reactive oxygen species (ROS), lipid disorder. Based on total motility (TM) integrity (SYBR14+/PI−), hierarchically (p < 0.001) clustered groups good (GFE) poor (PFE) freezability. PON1, SOD, TEAC higher 0.05) GFE than PFE, showing a positive some (PON1 TEAC) (SOD integrity. In contrast, OSI PFE that GFE, negatively correlated positively percentage viable high conclusion, SOD) (TEAC) are related cryotolerance. addition, our results suggest PON1 could be main enzymes involved control ROS species. Further investigation is needed confirm potential use these SP-antioxidants predict cryotolerance horses.

Language: Английский

---

Alpha-lipoic acid improves the quality of ram spermatozoa stored at 4°C by reducing oxidative stress and increasing mitochondrial potential

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 10

Published: Jan. 8, 2024

Introduction Ram spermatozoa inevitably produce a large number of reactive oxygen species (ROS) during liquid storage, leading to oxidative stress and decline quality. Therefore, it is particularly important add exogenous antioxidants the process semen preservation. The purpose this study investigate whether adding alpha-lipoic acid (ALA) ram can reduce enhance quality storage at 4°C. Methods Different concentrations ALA (0, 0.025, 0.05, 0.1, 0.5, 1 mM) were added stored During 4°C, motility, kinetic parameters, membrane integrity, acrosome energy metabolism parameters (mitochondrial potential (ΔΨM) adenosine triphosphate (ATP)) [ROS, malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD)] assessed. Results discussion results indicated that 0.1 mM significantly ( p &lt;0.05) improved motility (TM) progressive (PM), plasma ΔΨM, ATP, TAC, SOD, while reducing ROS MDA content compared control group. In conclusion, damage caused by in effectively improve preserved And optimal concentration mM.

Language: Английский

---

Preservative Effects of Curcumin on Semen of Hu Sheep

Animals, Journal Year: 2024, Volume and Issue: 14(6), P. 947 - 947

Published: March 19, 2024

Reactive oxygen species (ROS) are important factors that lead to a decline in sperm quality during semen preservation. Excessive ROS accumulation disrupts the balance of antioxidant system and causes lipid oxidative damage, destroying its structure function. Curcumin is natural plant extract neutralizes enhances function endogenous enzymes. The effect curcumin on preservation sheep has not been reported. This study aims determine effects refrigerated (4 °C) analyze metabolism from Chinese native (Hu sheep). results showed adding significantly improved (p < 0.05) viability at an optimal concentration 20 µmol/L, plasma membrane acrosome integrity were 0.05). Adding increased levels enzymes (T-AOC, CAT, SOD) decreased production. A total 13,796 metabolites 20,581 negative groups curcumin-supplemented identified using liquid chromatography–mass spectrometry. proportion lipids lipid-like molecules among all was highest, regardless treatment. We 50 differentially expressed (DEMs) between control curcumin-treated groups. Kyoto Encyclopedia Genes Genomes (KEGG) analysis revealed DEMs mainly enriched calcium signaling pathway, phospholipase D sphingolipid metabolism, steroid hormone biosynthesis, 2-oxocarboxylic acid other metabolic pathways. findings indicate addition appropriate (20 µm/L) can effectively suppress reactive production extend duration cryopreservation by modulating expression sphingosine-1-phosphate, dehydroepiandrosterone sulfate, phytosphingosine, semen. discovery offers novel approach enhancing cryogenic

Language: Английский

---

Supplementation of sperm cooling medium with Eurycoma longifolia extract enhances native Thai chicken sperm quality and fertility potential

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: Sept. 4, 2024

Cooled semen storage methods result in oxidative stress generated by an imbalance between oxidation rates, specifically reactive oxygen species production, and sperm cell antioxidants, leading to degradation of quality. We aimed investigate the impact adding Eurycoma longifolia (EL) extract as antioxidant supplement medium (IGGKPh extender) on quality fertility potential. EL at concentrations 5, 10, 15, 20 mg/mL was assessed for its capacity IGGKPh extender. Our findings revealed that total phenolic content did not vary significantly across various temperatures tested. However, incubation 5°C found be most effective temperature increasing via 2,2-diphenyl-1-picrylhydrazyl inhibition assay a dose-dependent manner. Supplementation extender with 15 enhance during cold up 48 h (

Language: Английский

---

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Cells, Journal Year: 2022, Volume and Issue: 11(18), P. 2845 - 2845

Published: Sept. 12, 2022

Preservation of human spermatozoa in vitro at normothermia or hypothermia maintaining their functions and fertility for several days plays a significant role reproductive biology medicine. However, it is well known that left deteriorate over time irreversibly as the consequence various stresses such change osmolarity, energy deficiency, oxidative damage, leading to substantial limitations including need semen examinations, preservation, assisted technology. These problems may be addressed with aid non-freezing storage techniques. The main most effective preservation strategies are partial total replacement seminal plasma culture medium, named extenders, temperature-induced metabolic restriction. Semen extenders consist buffers, osmolytes, antioxidants, etc. protect against above-mentioned adverse factors. Extended has negative effect on sperm parameters, whereas its ART outcomes remains inconsistent. duration, temperature, pre-treatment should determined according aims preservation. Advanced techniques nanotechnology omics have been introduced show great potential lifespan extension sperm. It certain more patients will benefit from near future. This review provided an overview current knowledge prospects prolonged vitro.

Language: Английский

---

Epicatechin Prevents Cryocapacitation of Bovine Spermatozoa through Antioxidant Activity and Stabilization of Transmembrane Ion Channels

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2510 - 2510

Published: Jan. 28, 2023

Epicatechin (EPC) is a flavonoid belonging to the family of catechins; it has been described as powerful scavenger wide spectrum reactive oxygen species (ROS) and modulator ex vivo sperm vitality. In this study, we assessed potential protective abilities EPC on cryopreserved bovine spermatozoa. We focused conventional quality parameters, well oxidative profile spermatozoa alongside capacitation patterns, expression profiles proteins involved in process capacitation. Semen samples were presence 25, 50 or 100 μmol/L compared native semen (negative control) ejaculates frozen absence (positive control). A dose-dependent improvement parameters was observed following administration, particularly case motility, membrane, acrosome DNA integrity comparison positive control. Experimental groups exposed all doses presented with significantly lower proportion capacitated opposed While no significant effects cases superoxide production, decrease levels hydrogen peroxide hydroxyl radical recorded experimental supplemented EPC. Western blot analysis revealed that supplementation extender prevented loss cation channel (CatSper) isoforms 1 2, sodium bicarbonate cotransporter (NBC) protein kinase (PKA), which play important roles summary, may hypothesize effective stabilization membrane during freeze-thaw through its ability quench ROS damage lipids prevent channels crucial initiate These attributes provide an additional layer protection low temperatures, be translated into higher post-thaw structural functional activity male gametes.

Language: Английский

---

Beneficial effect of L‐ Proline supplementation on the quality of human spermatozoa

Andrologia, Journal Year: 2022, Volume and Issue: 54(8)

Published: June 18, 2022

L-Proline is a natural anti-oxidative and osmoprotectant agent, playing versatile role in cell metabolism physiology. The present study aimed to explore the antioxidant effects of on human sperm function during incubation. Thirty healthy, normozoospermic men (27–40 years) were enrolled. Sperm samples incubated an unsupplemented medium (control group), or supplemented with (1, 2 4 mmol/L) evaluate its effect 0, 1, 24 h assessed terms motility, viability, morphology, chromatin DNA integrity. Moreover, levels reactive oxygen species (ROS), malondialdehyde (MDA), total capacity (TAC) determined medium. results indicated that mmol/L significantly improved maintenance normal integrity, TAC compared control group incubation (p < 0.05). However, 1 could not preserve parameters, quality, status different times > Collectively, inclusion (2 maintains parameters quality probably by modulating oxidative status.

Language: Английский

---