Tumor cell-intrinsic BIN1 deficiency promotes the immunosuppression and impedes ferroptosis of non-small cell lung cancer via G3BP1-mediated degradation of STAT1 DOI
Jiali Wang,

Yunlong Jia,

Tianxu Liu

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Abstract Background Tumors often evade immune surveillance by limiting T cell infiltration. In non-small lung cancer (NSCLC), increased infiltration of CD8+ cells is associated with a favorable response to immunotherapy. While BIN1 recognized as tumor suppressor gene, its role in shaping the microenvironment NSCLC has yet be fully clarified. Methods To investigate relationship between expression and CD8+T NSCLC, we performed comprehensive data analysis utilizing clinical information from patients. levels tissues were evaluated, their correlation patient survival outcomes was examined. Loss-of-function strategies targeting applied syngeneic mouse models assess functional significance. Tumor growth monitored, populations analyzed terms frequency functionality through mass cytometry flow techniques. Cytokine secretion profiled using multiplex assays. Additionally, RNA sequencing, immunoprecipitation-mass spectrometry, molecular docking employed confirm direct interactions cytokine-encoding genes. Finally, regulatory ferroptosis explored metabolomics analysis, ROS measurement, MDA detection. Results We observed that downregulated tissues, reduced strongly advanced disease progression poor prognosis. Bioinformatics human samples revealed positive Furthermore, prognostic impact on patients linked level models, knockout significantly inhibited impaired cytotoxic function, facilitating evasion. Mechanistically, demonstrated directly interacts G3BP1, stabilizes G3BP1. This, turn, promotes STAT1 degradation reduces cell-recruiting chemokines such CXCL10 CCL5. our findings reveal influences G3BP1/STAT1/GSH pathway, thereby regulating proliferation, migration, invasion. Conclusion This study highlights crucial BIN1/G3BP1/STAT1/CD8+ tumor-infiltrating lymphocyte signaling pathway mechanisms fundings lay foundation for development BIN1-targeted therapies aimed at improving immunogenicity transforming immunologically “cold” into more responsive disease.

Language: Английский

A Review of the Therapeutic Potential of Sulfur Compounds in Allium sativum DOI Creative Commons
Agatha Ngukuran Jikah, Great Iruoghene Edo,

Raghda Makia

et al.

Measurement Food, Journal Year: 2024, Volume and Issue: 15, P. 100195 - 100195

Published: Aug. 28, 2024

Language: Английский

Citations

12
GSH and Ferroptosis: Side-by-Side Partners in the Fight against Tumors DOI Creative Commons

Yulang Jiang,

Christian Glandorff, Mingyu Sun

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(6), P. 697 - 697

Published: June 6, 2024

Glutathione (GSH), a prominent antioxidant in organisms, exhibits diverse biological functions and is crucial safeguarding cells against oxidative harm upholding stable redox milieu. The metabolism of GSH implicated numerous diseases, particularly the progression malignant tumors. Consequently, therapeutic strategies targeting regulation synthesis to modulate levels represent promising avenue for future research. This study aimed elucidate intricate relationship between ferroptosis, highlighting how modulation can impact cellular susceptibility ferroptosis consequently influence development tumors other diseases. paper provides comprehensive overview physiological GSH, including its structural characteristics, physicochemical properties, sources, metabolic pathways, as well investigate molecular mechanisms underlying potential interventions. Unraveling role holds promise individuals afflicted with

Language: Английский

Citations

9
A ROS-responsive TPP-modified tanshinone IIA micelle improves DOX-induced heart failure DOI
Yan Zhao, Jiahua Wang,

Zixu Zhang

et al.

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: 672, P. 125318 - 125318

Published: Feb. 5, 2025

Language: Английский

Citations

1
Glutathione-Dependent Pathways in Cancer Cells DOI Open Access
Elena Kalinina

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8423 - 8423

Published: Aug. 1, 2024

The most abundant tripeptide—glutathione (GSH)—and the major GSH-related enzymes—glutathione peroxidases (GPxs) and glutathione S-transferases (GSTs)—are highly significant in regulation of tumor cell viability, initiation development, its progression, drug resistance. high level GSH synthesis different cancer types depends not only on increasing expression key enzymes γ-glutamyl cycle but also changes transport velocity precursor amino acids. ability GPxs to reduce hydroperoxides is used for cellular each member GPx family has a mechanism action site maintaining redox balance. GSTs catalyze conjugation electrophilic substances reduction organic take part signaling pathways. By catalyzing S-glutathionylation target proteins, are involved processes, including metabolism (e.g., glycolysis PPP), signal transduction, transcription regulation, development resistance anticancer drugs. In this review, recent findings synthesis, roles functions GPxs, GST isoforms discussed, along with search inhibitors treatment.

Language: Английский

Citations

5
The Role of Dietary Antioxidants, Food Supplements and Functional Foods for Energy Enhancement in Healthcare Professionals DOI Creative Commons

Theodora Kalogerakou,

Μαρία Αντωνιάδου

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1508 - 1508

Published: Dec. 10, 2024

Healthcare professionals frequently experience significant work overload, which often leads to substantial physical and psychological stress. This stress is closely linked increased oxidative a corresponding decline in energy levels. scoping review investigates the potential impact of dietary antioxidants food supplements conjunction with diet controlling these negative effects. Through an analysis biochemical pathways involved metabolism, paper emphasizes effectiveness targeted interventions. Key antioxidants, such as vitamins C E, polyphenols, carotenoids, are evaluated for their ability counteract enhance Additionally, assesses various supplements, including omega-3 fatty acids, coenzyme Q10, ginseng, mechanisms action enhancement. Practical guidelines incorporating energy-boost strategies into routine healthcare provided, emphasizing importance modifications reducing improving overall well-being performance high-stress environments. The concludes by suggesting directions future research validate findings explore new interventions that may further support under overload.

Language: Английский

Citations

5
Prolactin is an Endogenous Antioxidant Factor in Astrocytes That Limits Oxidative Stress-Induced Astrocytic Cell Death via the STAT3/NRF2 Signaling Pathway DOI Creative Commons

Miriam Ulloa,

Fernando Macías, Carmen Clapp

et al.

Neurochemical Research, Journal Year: 2024, Volume and Issue: 49(7), P. 1879 - 1901

Published: May 17, 2024

Abstract Oxidative stress-induced death of neurons and astrocytes contributes to the pathogenesis numerous neurodegenerative diseases. While significant progress has been made in identifying neuroprotective molecules against neuronal oxidative damage, little is known about their counterparts for astrocytes. Prolactin (PRL), a hormone stimulate astroglial proliferation, viability, cytokine expression, exhibits antioxidant effects neurons. However, its role protecting from stress remains unexplored. Here, we investigated effect PRL hydrogen peroxide (H 2 O )-induced insult primary cortical astrocyte cultures. Incubation with led increased enzymatic activity superoxide dismutase (SOD) glutathione peroxidase (GPX), resulting higher total capacity. Concomitantly, prevented H -induced cell death, reactive oxygen species accumulation, protein lipid oxidation. The protective upon can be explained by activation both signal transducer activator transcription 3 (STAT3) NFE2 like bZIP factor (NRF2) transduction cascades. We demonstrated that induced nuclear translocation transcriptional upregulation Nrf2 , concurrently NRF2-dependent genes heme oxygenase 1, Sod1, Sod2 Gpx1 . Pharmacological blockade STAT3 suppressed PRL-induced Sod1 mRNA, SOD GPX activities. Furthermore, genetic ablation receptor susceptibility while diminishing intrinsic Overall, these findings unveil as potent protects insult, which may contribute brain neuroprotection.

Language: Английский

Citations

4
Pharmacokinetics, mass balance, and metabolism of [14C]PLB1004, a selective and irreversible EGFR-TKI in humans DOI
Donghui Liu, Qian Li, Yan Shu

et al.

Cancer Chemotherapy and Pharmacology, Journal Year: 2025, Volume and Issue: 95(1)

Published: Jan. 7, 2025

Language: Английский

Citations

0
Multi-trace elements-enriched functional drink accelerates gastric ulcer repair via the HGF/c-Met/STAT3 pathway DOI

Yongnan Piao,

Nuoya Wang,

Mingji Jin

et al.

Journal of Functional Foods, Journal Year: 2025, Volume and Issue: 125, P. 106674 - 106674

Published: Jan. 18, 2025

Language: Английский

Citations

0
Enhanced methanogenesis and efficient ciprofloxacin degradation via nZVI@LDH in an electricity-driven anaerobic bioreactor: A biotic-abiotic hybrid system for ROS regulation and ARGs mitigation DOI
Jinfeng Chen, Yi Tang, Xinyan Chen

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 137348 - 137348

Published: Jan. 1, 2025

Language: Английский

Citations

0
Selenium Mitigates Caerulein and LPS-induced Severe Acute Pancreatitis by Inhibiting MAPK, NF-κB, and STAT3 Signaling via the Nrf2/HO-1 Pathway DOI

K Swetha,

Mylanayakanahosahalli Chandrashekar Indumathi,

R Sanchit Kishan

et al.

Biological Trace Element Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Language: Английский

Citations

0