Hepatology International, Journal Year: 2022, Volume and Issue: 16(4), P. 741 - 754
Published: June 1, 2022
Language: Английский
Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)
Published: Feb. 17, 2023
Abstract Drug resistance is mainly responsible for cancer recurrence and poor prognosis. Epigenetic regulation a heritable change in gene expressions independent of nucleotide sequence changes. As the common epigenetic mechanisms, DNA methylation, histone modification, non-coding RNA have been well studied. Increasing evidence has shown that aberrant regulations contribute to tumor resistance. Therefore, targeting regulators represents an effective strategy reverse drug In this review, we summarize roles addition, as essential factors modifications, demethylases mediate or genomic modifications. Herein, comprehensively describe functions demethylase family including lysine-specific family, Jumonji C-domain-containing arginine fully discuss their regulatory mechanisms related therapeutic strategies, small-molecule inhibitors small interfering overcome resistance, are also described.
Language: Английский
Citations
159
Journal of Hematology & Oncology, Journal Year: 2022, Volume and Issue: 15(1)
Published: March 2, 2022
Non-coding RNAs (ncRNAs) are a large family of RNA molecules with no capability in encoding proteins. However, they participate developmental and biological processes their abnormal expression affects cancer progression. These can function as upstream mediators different signaling pathways enhancer zeste homolog 2 (EZH2) is among them. Briefly, EZH2 belongs to PRCs exert functional roles cells due its methyltransferase activity. gene via inducing H3K27me3. In the present review, our aim provide mechanistic discussion ncRNAs role regulating cancers. MiRNAs dually induce/inhibit affect downstream targets such Wnt, STAT3 EMT. Furthermore, miRNAs regulate therapy response affecting signaling. It noteworthy that reduce miRNA by binding promoter exerting Small-interfering (siRNA) short-hairpin (shRNA) synthetic, short capable reducing suppressing LncRNAs mainly targeting miRNAs. lncRNAs induce modulating expression. Circular (CircRNAs), like lncRNAs, areas discussed review focus on molecular leading clinical translation.
Language: Английский
Citations
138
Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13
Published: April 4, 2022
Background AlkB homolog 5 (ALKBH5) is a N 6 -methyladenosine (m A) demethylase associated with the development, growth, and progression of multiple cancer types. However, biological role ALKBH5 has not been investigated in pan-cancer datasets. Therefore, this study, comprehensive bioinformatics analysis datasets was performed to determine mechanisms through which regulates tumorigenesis. Methods Online websites databases such as NCBI, UCSC, CCLE, HPA, TIMER2, GEPIA2, cBioPortal, UALCAN, STRING, SangerBox, ImmuCellAl, xCell, GenePattern were used extract data cancers. The patient analyzed relationship between expression, genetic alterations, methylation status, tumor immunity. Targetscan, miRWalk, miRDB, miRabel, LncBase Cytoscape tool identify microRNAs (miRNAs) long non-coding RNAs (lncRNAs) that regulate expression construct lncRNA-miRNA-ALKBH5 network. In vitro CCK-8, wound healing, Transwell M2 macrophage infiltration assays well vivo xenograft animal experiments functions glioma cells. Results showed upregulated several solid tumors. significantly correlated prognosis patients. Genetic alterations including duplications deep mutations gene identified Alterations prognosis. GO KEGG enrichment analyses ALKBH5-related genes enriched inflammatory, metabolic, immune signaling pathways glioma. checkpoint (ICP) genes, influenced sensitivity immunotherapy. We constructed lncRNA-miRNA network development progression. promoted proliferation, migration, invasion cells recruited Conclusions overexpressed types cancers regulation tumor-infiltration Our study shows promising prognostic immunotherapeutic biomarker some malignant
Language: Английский
Citations
79
Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9
Published: April 9, 2021
Long non-coding RNAs (lncRNAs) have gained great attention as epigenetic regulators of gene expression in many tissues. Increasing evidence indicates that lncRNAs, together with microRNAs (miRNAs), play a pivotal role osteogenesis. While miRNA action mechanism relies mainly on miRNA-mRNA interaction, resulting suppressed expression, lncRNAs affect mRNA functionality through different activities, including interaction miRNAs. Recent advances RNA sequencing technology improved knowledge into the molecular pathways regulated by and This review reports recent miRNAs roles key osteogenic differentiation. Specifically, we described herein discoveries lncRNA-miRNA crosstalk during differentiation mesenchymal stem cells (MSCs) derived from bone marrow (BM), well other anatomical regions. The deep understanding connection between will strongly improve mechanisms growth development, ultimately leading to discover innovative diagnostic therapeutic tools for disorders diseases.
Language: Английский
Citations
95
Genes, Journal Year: 2021, Volume and Issue: 12(8), P. 1289 - 1289
Published: Aug. 23, 2021
Since the discovery of first microRNA (miRNA), exploration miRNA biology has come to a new era in recent decades. Monumental studies have proven that miRNAs can be dysregulated different types cancers and roles turn out function either tumor promoters or suppressors. The interplay between development grabbed attention as novel tools targets for therapeutic attempts. Moreover, delivery system accelerates preclinical implications. In this review, we depict advances cancer discuss potential diagnostic approaches miRNAs.
Language: Английский
Citations
86
Frontiers in Molecular Neuroscience, Journal Year: 2021, Volume and Issue: 14
Published: Oct. 5, 2021
Microglia and astrocytes maintain tissue homeostasis in the nervous system. Both microglia have pro-inflammatory phenotype anti-inflammatory phenotype. Activated activated can contribute to several neurological diseases. Long non-coding RNAs (lncRNAs) circular (circRNAs), two groups of (ncRNAs), function as competing endogenous (ceRNAs) impair microRNA (miRNA) inhibition on targeted messenger (mRNAs). LncRNAs circRNAs are involved various disorders. In this review, we summarized that lncRNAs participate dysfunction, astrocyte neuron damage, inflammation. Thereby, positively or negatively regulate diseases, including spinal cord injury (SCI), traumatic brain (TBI), ischemia-reperfusion (IRI), stroke, neuropathic pain, epilepsy, Parkinson’s disease (PD), multiple sclerosis (MS), Alzheimer’s (AD). Besides, also found a lncRNA/circRNA-miRNA-mRNA regulatory network mediated Through hope cast light mechanisms diseases provide new insights for treatment.
Language: Английский
Citations
80
Hepatology, Journal Year: 2021, Volume and Issue: 74(6), P. 3213 - 3234
Published: July 29, 2021
Oxaliplatin (OXA) is one of the most common chemotherapeutics in advanced hepatocellular carcinoma (HCC), resistance which poses a big challenge. Long noncoding RNAs (lncRNAs) play vital roles chemoresistance. Therefore, elucidating underlying mechanisms and identifying predictive lncRNAs for OXA needed urgently.RNA sequencing (RNA-seq) fluorescence situ hybridization (FISH) were used to investigate OXA-resistant (OXA-R) lncRNAs. Survival analysis was performed determine clinical significance homo sapiens long intergenic non-protein-coding RNA 1134 (LINC01134) p62 expression. Luciferase, immunoprecipitation (RIP), chromatin (ChIP), isolation by purification (ChIRP) assays explore LINC01134 regulates The effects LINC01134/SP1/p62 axis on evaluated using cell viability, apoptosis, mitochondrial function morphology analysis. Xenografts estimate vivo regulation axis. ChIP, xenograft identify demethylase up-regulation resistance.LINC01134 identified as up-regulated OXA-R cells. Higher expression predicted poorer therapeutic efficacy. activates anti-oxidative pathway through recruiting transcription factor SP1 promoter. altering homeostasis both vitro vivo. Furthermore, demethylase, lysine specific 1 (LSD1) responsible In patients with HCC, positively correlated LSD1 expressions, whereas expression.LSD1/LINC01134/SP1/p62 critical HCC. Evaluating HCC will be effective predicting treatment-naive patients, targeting may promising strategy overcome
Language: Английский
Citations
75
Theranostics, Journal Year: 2022, Volume and Issue: 12(15), P. 6626 - 6645
Published: Jan. 1, 2022
Ischemic disease is a class of diseases in which an organ ischemic due to vascular occlusion, major contributor death and disability worldwide.However, when the blood flow restored, more severe damage occurs than ischemia alone known as ischemic-reperfusion injury (IRI).During reperfusion, imbalance between production reactive oxygen species (ROS) buffering capacity antioxidant defense system results cell death.Nuclear factor E2-related 2 (Nrf2) significantly affects stress damage.The function Nrf2 pathological process IRI has been widely discussed, but impact epigenetic modifications associated with remains unclear.This article provides comprehensive overview role mechanism Nrf2-related various organs, including brain, heart, liver, kidney.In addition, we summarize agonists that may target regulation Nrf2, be beneficial seeking effective strategies improve IRI.
Language: Английский
Citations
49
Biomarker Research, Journal Year: 2023, Volume and Issue: 11(1)
Published: Jan. 4, 2023
Metastasis is a significant factor that affects the survival of patients with non-small cell lung cancer (NSCLC). Nevertheless, molecular regulatory mechanism underlying metastasis currently not fully understood. This study aims to identify important role miR-124-3p in NSCLC, thereby providing potential therapeutic intervention.Exosome secretion was determined by Nanoparticle Tracking Analysis (NTA) and uptake measured fluorescence inverted microscope. The binding between its upstream or downstream target genes validated experimentally Luciferase reporter. Cells migration evaluated transwell assays. Transcriptome sequencing on A549 carried out verify signaling pathway regulation. Western blotting analysis used assess level AKT, p-AKT, PI3K, p-PI3K protein expression NSCLC lines. suppress tumor verified xenograft model.Exosomes were more abundant serum from advanced (n = 24 patients) than these early-stage 30 patients), which suggested correlation amount exosome NSCLC. Interestingly, release, cells notably inhibited miR-124-3p. LINC00511 suppressed facilitate transport due as competitive endogenous RNA for could directly 3'-UTR Rab27a inhibit prevent invasion. Aside inhibition transport, activation PI3K/AKT intracellular environment. Finally, measuring subcutaneous weight volume metastasis, we also demonstrated growth vivo.In significantly through extracellular signaling. These findings provide new insights toward better understanding suggest treatment biomarker
Language: Английский
Citations
34
Non-Coding RNA, Journal Year: 2023, Volume and Issue: 9(4), P. 38 - 38
Published: July 4, 2023
Circular RNAs (circRNAs) represent single-stranded RNA species that contain covalently closed 3′ and 5′ ends provide them more stability than linear RNA, which has free ends. Emerging evidence indicates circRNAs perform essential functions in many DNA viruses, including coronaviruses, Epstein–Barr cytomegalovirus, Kaposi sarcoma viruses. Recent studies have confirmed are present play various important such as evading host immune response, disease pathogenesis, protein translation, miRNA sponges, regulating cell proliferation, virus replication. Studies can be biological signatures or pathological markers for autoimmune diseases, neurological cancers. However, our understanding of viruses is still limited, functional evaluation viral to completely understand their functions. In the review, we describe metabolism cellular roles circRNA, its diseases circRNA found interact with proteins, DNA, thus modulate processes, transcription, splicing, other interfere signaling pathways take part vital biological, physiological, cellular, pathophysiological processes. We also summarize recent demonstrating circRNA’s this growing field research.
Language: Английский
Citations
33