Biomarker discovery and application—An opportunity to resolve the challenge of liver cancer diagnosis and treatment DOI Creative Commons
Jingtao Chen, Chao Niu, Ning Yang

et al.

Pharmacological Research, Journal Year: 2023, Volume and Issue: 189, P. 106674 - 106674

Published: Jan. 23, 2023

Liver cancer is one of the most common malignancies, with severe morbidity and mortality. While considerable progress has been made in liver treatment, 5-year overall survival (OS) patients not improved significantly. Reasons include inadequate capability early screening diagnosis, a high incidence recurrence metastasis, degree tumor heterogeneity, an immunosuppressive microenvironment. Therefore, identification validation specific robust biomarkers are major importance for screening, timely accurate prognosis, prevention progression. In this review, we highlight some latest research potential applications biomarkers, describing hotspots prospective directions biomarker discovery.

Language: Английский

Advances and challenges in portable optical biosensors for onsite detection and point-of-care diagnostics DOI
Sufian Rasheed,

Tehreem Kanwal,

Naseer Ahmad

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 173, P. 117640 - 117640

Published: March 8, 2024

Language: Английский

Citations

67
Toll-like receptor-guided therapeutic intervention of human cancers: molecular and immunological perspectives DOI Creative Commons
Suprabhat Mukherjee, Ritwik Patra, Payam Behzadi

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 26, 2023

Toll-like receptors (TLRs) serve as the body’s first line of defense, recognizing both pathogen-expressed molecules and host-derived released from damaged or dying cells. The wide distribution different cell types, ranging epithelial to immune cells, highlights crucial roles TLRs in linking innate adaptive immunity. Upon stimulation, binding mediates expression several adapter proteins downstream kinases, that lead induction other signaling such key pro-inflammatory mediators. Indeed, extraordinary progress immunobiological research has suggested could represent promising targets for therapeutic intervention inflammation-associated diseases, autoimmune microbial infections well human cancers. So far, prevention possible treatment inflammatory various TLR antagonists/inhibitors have shown be efficacious at stages pre-clinical evaluation clinical trials. Therefore, fascinating role modulating responses levels directed scientists opt these sensor suitable developing chemotherapeutics immunotherapeutics against cancer. Hitherto, TLR-targeting small (e.g., Pam3CSK4, Poly (I:C), (A:U)), chemical compounds, phytocompounds Curcumin), peptides, antibodies been found confer protection types However, administration inappropriate doses TLR-modulating therapeutics a wrong infusion is reported induce detrimental outcomes. This review summarizes current findings on molecular structural biology gives an overview potency promises TLR-directed strategies cancers by discussing established pipeline discoveries.

Language: Английский

Citations

65
Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression DOI
Shuai Wang,

Lingyi Zhou,

Ning Ji

et al.

Drug Resistance Updates, Journal Year: 2023, Volume and Issue: 69, P. 100976 - 100976

Published: May 17, 2023

Language: Английский

Citations

52
Heterogeneity of hepatocellular carcinoma: from mechanisms to clinical implications DOI Creative Commons
Fatema Safri,

Romario Nguyen,

Shadi Zerehpooshnesfchi

et al.

Cancer Gene Therapy, Journal Year: 2024, Volume and Issue: 31(8), P. 1105 - 1112

Published: March 18, 2024

Abstract Hepatocellular Carcinoma (HCC) is one of the most common types primary liver cancer. Current treatment options have limited efficacy against this malignancy, primarily owing to difficulties in early detection and inherent resistance existing drugs. Tumor heterogeneity a pivotal factor contributing significantly recurrent manifestations HCC. Intratumoral an important aspect spectrum complex tumor contributes late diagnosis failure. Therefore, it crucial thoroughly understand molecular mechanisms how develops. This review aims summarize possible dimensions with emphasis on intratumoral heterogeneity, evaluate its profound impact therapeutic strategies for HCC, explore suitability appropriate pre-clinical models that can be used best study heterogeneity; thus, opening new avenues cancer treatment.

Language: Английский

Citations

29
Interaction of STIL with FOXM1 regulates SF3A3 transcription in the hepatocellular carcinoma development DOI Creative Commons
Haijun Zhang, Lin Zhang, Zhichao Wu

et al.

Cell Division, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 17, 2025

Dysregulation of SF3A3 has been related to the development many cancers. Here, we investigated functional role in hepatocellular carcinoma (HCC). expression HCC tissues and cell lines was examined using RT-qPCR. Changes malignant behavior cells after downregulation were assessed by EdU, colony formation, flow cytometry, wound healing, Transwell invasion assays. Multiple datasets combined identify upstream modifiers SF3A3. The binding relationship between STIL FOXM1 explored co-IP assay, effect on at promoter detected ChIP-qPCR assay. A xenograft tumor model established explore changes tumors vivo, Ki67, GPC3, p53 immunohistochemistry. overexpressed cells, or inhibited promoting p53. An interaction regulated cells. Knockdown further enhanced anti-tumor effects loss vitro whereas overexpression overturned impact vivo. Our findings indicate that STIL/FOXM1 expedites activating SF3A3, which highlights importance as a promising prognostic marker therapeutic target for HCC.

Language: Английский

Citations

2
Highly Sensitive Digital and Analog Immunoassay Based on Submicron Magnetic Beads and Fluorescent Microspheres DOI
Feng Gong,

Xiaoyun Shan,

Kebing Yi

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

The detection of disease-related protein biomarkers plays a crucial role in the early diagnosis, treatment, and monitoring diseases. concentrations can vary significantly different diseases or stages same disease. However, most existing analytical methods cannot simultaneously meet requirements high sensitivity wide dynamic range. Herein, we developed digital analog immunoassay method based on submicron magnetic beads fluorescent microspheres. analysis achieves limit as low 46 fg/mL (1.8 fM) for IL-6, has range spanning from 0.2 pg/mL to 10 ng/mL. Furthermore, be used quickly roughly assess higher concentration proteins via visual detection, practical application potential this was verified by alpha-fetoprotein serum samples 12 healthy individuals hepatocellular carcinoma patients. established does not involve use enzymes costly instruments, which greatly simplifies experimental steps, shortens time, reduces cost. In view those advantages, proposed great prospects point-of-care applications.

Language: Английский

Citations

2
Identification of lncRNA/circRNA-miRNA-mRNA ceRNA Network as Biomarkers for Hepatocellular Carcinoma DOI Creative Commons
Shanshan Chen, Yongchao Zhang, Xiaoyan Ding

et al.

Frontiers in Genetics, Journal Year: 2022, Volume and Issue: 13

Published: March 21, 2022

Background: Hepatocellular carcinoma (HCC) accounts for the majority of liver cancer, with incidence and mortality rates increasing every year. Despite improvement clinical management, substantial challenges remain due to its high recurrence short survival period. This study aimed identify potential diagnostic prognostic biomarkers in HCC through bioinformatic analysis. Methods: Datasets from GEO TCGA databases were used Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses carried out by WebGestalt website clusterProfiler package R. The STRING database Cytoscape software establish protein-protein interaction (PPI) network. GEPIA was perform expression genes. miRDB, miRWalk, TargetScan employed predict miRNAs levels predicted explored via OncomiR database. LncRNAs StarBase LncBase while circRNA prediction performed circBank. ROC curve analysis Kaplan-Meier (KM) evaluate value gene expression, respectively. Results: A total 327 upregulated 422 downregulated overlapping DEGs identified between tissues noncancerous tissues. PPI network constructed 89 nodes 178 edges eight hub genes selected upstream ceRNAs. lncRNA/circRNA-miRNA-mRNA successfully based on ceRNA hypothesis, including five lncRNAs (DLGAP1-AS1, GAS5, LINC00665, TYMSOS, ZFAS1), six circRNAs (hsa_circ_0003209, hsa_circ_0008128, hsa_circ_0020396, hsa_circ_0030051, hsa_circ_0034049, hsa_circ_0082333), (hsa-miR-150-5p, hsa-miR-19b-3p, hsa-miR-23b-3p, hsa-miR-26a-5p, hsa-miR-651-5p, hsa-miR-10a-5p, hsa-miR-214-5p hsa-miR-486-5p), mRNAs (CDC6, GINS1, MCM4, MCM6, MCM7). can promote progression cell cycle, DNA replication, other pathways. Clinical demonstrated that ZFAS1/hsa-miR-150-5p/GINS1 regulatory axis had a value. Conclusion: These results revealed cycle replication pathway could be pathways participate development. is expected provide therapeutic targets especially axis.

Language: Английский

Citations

57
Development and experimental verification of a prognosis model for cuproptosis-related subtypes in HCC DOI

Yixiu Wang,

Yongfa Zhang, Longrong Wang

et al.

Hepatology International, Journal Year: 2022, Volume and Issue: 16(6), P. 1435 - 1447

Published: Sept. 5, 2022

Language: Английский

Citations

43
The SNHG1-Centered ceRNA Network Regulates Cell Cycle and Is a Potential Prognostic Biomarker for Hepatocellular Carcinoma DOI Open Access
Lin Zhou, Qing Zhang,

Haibin Deng

et al.

The Tohoku Journal of Experimental Medicine, Journal Year: 2022, Volume and Issue: 258(4), P. 265 - 276

Published: Jan. 1, 2022

Hepatocellular carcinoma (HCC) is one of the most common and lethal types cancer. This study aimed to identify expression regulatory network a prognostic signature HCC. RNA-seq data from The Cancer Genome Atlas were used differentially expressed genes (DEGs) between HCC normal liver tissues. DEGs subjected construction protein-protein interaction (PPI) enrichment analysis Gene Ontology terms Kyoto Encyclopedia Genes Genomes pathways. results showed that enriched in cell cycle pathway, top 10 hub PPI belong pathway. A ceRNA was constructed using starBase database, including lncRNA (SNHG1), seven miRNAs (miR-195-5p, miR-199a-3p, miR-199a-5p, miR-199b-3p, miR-383-5p, miR-424-5p miR-654-3p) six (BUB1, CCNA2, CCNB1, KIF11, NCAPG, TOP2A). In vitro experiments knockdown SNHG1 lines (Huh7 HepG2) decreased viability, leading arrest at G1 phase. These findings indicate promotes proliferation by regulating cycle-related as ceRNA. Additionally, Kaplan-Meier's survival multivariate Cox regression identified (including SNHG1-regulated genes) for overall patients. conclusion, this novel potential independent factor

Language: Английский

Citations

42
The expression of cuproptosis-related genes in hepatocellular carcinoma and their relationships with prognosis DOI Creative Commons
Xueying Zhao, Jin Chen,

Shangqi Yin

et al.

Frontiers in Oncology, Journal Year: 2022, Volume and Issue: 12

Published: Oct. 14, 2022

The mechanism of cuproptosis has recently been reported in lipoylated proteins the tricarboxylic acid (TCA) cycle. Besides, role copper was previously recognized cancer progression. We evaluated prognostic value cuproptosis-related gene expression hepatocellular carcinoma (HCC). Remarkable genes were selected both differential analysis and Kaplan-Meier survival from ninety-six using Cancer Genome Atlas (TCGA) database. relationships between clinical characteristics performed with Wilcoxon signed-rank test, Kruskal-Wallis logistic regression. Clinicopathologic factors correlated overall HCCs conducting univariate multivariate Cox regression analysis. Gene Expression Profiling Interactive Analysis 2 (GEPIA2) Human Protein (HPA) databases utilized to verify results. Furthermore, Set Enrichment (GSEA) identified potential key pathways that dominate HCC. Elevated ATP7A, SLC25A3, SCO2, COA6, TMEM199, ATP6AP1, LIPT1, DLAT, PDHA1, MTF1, ACP1, FDX2, NUBP2, CIAPIN1, ISCA2 NDOR1 expression, as well declined AOC1, FDX1, MT-CO1, ACO1 significantly emerged HCC tumor tissues associated poor survival. expressions screened prominently related features. GSEA many signaling (such natural killer cell mediated cytotoxicity, TCA cycle, glutathione metabolism, ATP-binding cassette (ABC) transporters, Notch pathway, ErbB metabolism xenobiotics by cytochrome p450) differentially enriched varying degrees expression. twenty might be new candidate biomarkers for

Language: Английский

Citations

38