Characterization of plant laccase genes and their functions

Gene, Journal Year: 2022, Volume and Issue: 852, P. 147060 - 147060

Published: Nov. 21, 2022

Language: Английский

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MicroRNA408 negatively regulates salt tolerance by affecting secondary cell wall development in maize

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 192(2), P. 1569 - 1583

Published: March 2, 2023

Although microRNA408 (miR408) is a highly conserved miRNA, the miR408 response to salt stress differs among plant species. Here, we show that transcripts are strongly repressed by and methyl viologen treatment in maize (Zea mays). Application of N, N1-dimethylthiourea partly relieved NaCl-induced down-regulation miR408. Transgenic overexpressing MIR408b hypersensitive stress. Overexpression enhanced rate net Na+ efflux, caused locate inter-cellular space, reduced lignin accumulation, number cells vascular bundles under We further demonstrated targets ZmLACCASE9 (ZmLAC9). Knockout MIR408a or overexpression ZmLAC9 increased accumulation lignin, thickened walls pavement cells, improved tolerance maize. Transcriptome profiles wild-type MIR408b-overexpressing transgenic with without indicated negatively regulates expression cell wall biogenesis genes conditions. These results indicate regulating secondary development

Language: Английский

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Screening of cadmium resistant bacteria and their growth promotion of Sorghum bicolor (L.) Moench under cadmium stress

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 272, P. 116012 - 116012

Published: Jan. 30, 2024

Heavy metal pollution of agricultural soils, especially from cadmium (Cd) contaminationcaused serious problems in both food security and economy. Sorghum bicolor (L.) showed a great potential phytoremediation Cd contamination due to its fast growth, high yield easy harvesting. However, the growth S. plants tends be inhibited under exposure, which limited application for remediation. Plant growth-promoting rhizobacteria may enhance resistance thus improve removal efficiency. In this study, three Cd-resistant bacteria were screened based on acid tolerance identified as Bacillus velezensis QZG6, Enterobacter cloacae QZS3 cereus QZS8, by 16S rRNA sequencing. Inoculation hydroponic with strains or QZS8 significantly promoted biomass sorghum 31.52%, 50.20% 26.93%, respectively, compared those uninoculated exposure. The activity SOD, POD MDA content Cd-stressed reduced 65.74%, 80.91%, when inoculated QZS3. For pot experiment, 47.30%, 19.27% 58.47%, 67.20%, 22.40%, 40.65%, All these increased efficiency 42.16% (QZG6), 18.76% (QZS3) 21.06% (QZS8). To investigate bacterial characteristics associated promotion plants, ability nitrogen fixation, phosphorus solubilization, siderophores production, phytohormones production determined. able fix nitrogen. Phosphorus release was observed QZG6 (inorganic organic phosphorus) phosphorus). Both produce siderophores, while only positive ACC deaminase. produced IAA, SA GA. These results indicated that plant stress, probably through detoxification well regulation N/P nutrient supply phytohormone. present study combined remediation Cd-polluted provide new insight into combining advantages microbes Cd-contaminated soils.

Language: Английский

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sly‐miR408b Targets a Plastocyanin‐Like Protein to Regulate Mycorrhizal Symbiosis in Tomato

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

ABSTRACT Symbiosis between arbuscular mycorrhizal fungi and plants plays a crucial role in nutrient acquisition stress resistance for terrestrial plants. microRNAs have been reported to participate the regulation of symbiosis by controlling expression their target genes. Herein, we found that sly‐miR408b was significantly downregulated response colonisation. Overexpression compromised colonisation Rhizophagus irregularis tomato ( Solanum lycopersicum ) roots. A basic blue protein gene SlBBP then identified as new miR408b tomato. The membrane‐located induced copper‐dependent manner. Importantly, loss function decreased root SOD activity, which may interfere with process scavenging excessive reactive oxygen species (ROS). Mutation RBOH1 , encodes ROS‐producing enzymes NADPH oxidases, obviously reduced arbuscule abundance mutant Overall, our results provide evidence its regulate through mediating ROS production.

Language: Английский

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Neuroprotection and Mechanism of Gas-miR36-5p from Gastrodia elata in an Alzheimer’s Disease Model by Regulating Glycogen Synthase Kinase-3β

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(24), P. 17295 - 17295

Published: Dec. 9, 2023

Alzheimer’s disease (AD) is currently the most common neurodegenerative disease. Glycogen synthase kinase 3β (GSK-3β) a pivotal factor in AD pathogenesis. Recent research has demonstrated that plant miRNAs exert cross-kingdom regulation on target genes animals. Gastrodia elata (G. elata) valuable traditional Chinese medicine significant pharmacological activity against diseases of central nervous system (CNS). Our previous studies have indicated G. elata-specific miRNA plays regulatory role for NF-κB signaling pathway mice. In this study, further bioinformatics analysis suggested Gas-miR36-5p targets GSK-3β. Through western blot, RT-qPCR, and assessments T-AOC, SOD, MDA levels, its neuroprotective effects an cell model. Furthermore, was detected murine brain tissues. The results Morris water maze test blot provided positive evidence reversing learning deficits hyperphosphorylation Tau mice, elucidating model following RNA administration. emphasizes as novel with properties by targeting Consequently, our findings provide insights into mechanisms underlying miRNA, presenting perspective treatment

Language: Английский

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Riboflavin mediates m6A modification targeted by miR408, promoting early somatic embryogenesis in longan

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 192(3), P. 1799 - 1820

Published: March 17, 2023

Abstract Plant somatic embryogenesis (SE) is an in vitro biological process wherein bipolar structures are induced to form cells and regenerate into whole plants. MicroRNA (miRNA) essential player plant SE. However, the mechanism of microRNA408 (miR408) SE remains elusive. Here, we used stable transgenic technology longan (Dimocarpus longan) embryogenic calli verify by which miR408 promotes cell division differentiation early dlo-miR408-3p regulated riboflavin biosynthesis targeting nudix hydrolase 23 (DlNUDT23), a previously unidentified gene mediating N6-methyladenosine (m6A) modification influencing RNA homeostasis cycle expression during We showed that DlMIR408 overexpression (DlMIR408-OE) promoted 21-nt miRNA biosynthesis. In DlMIR408-OE lines, targeted downregulated DlNUDT23, biosynthesis, decreased flavin mononucleotide (FMN) accumulation, m6A level, influenced homeostasis. DNA replication, glycosylphosphatidylinositol (GPI)-anchor pentose phosphate pathway, taurine hypotaurine metabolism were also closely associated with metabolism. feeding assay, pre-miR408 upregulated DlNUDT23 was downregulated, increasing level globular embryos. When inhibited, upregulated, inhibited but did not inhibit differentiation. FMN artificial demethylated affected precursor miRNA. Our results revealed underlying dlo-miR408-3p-activated targeted, dynamically mediated, affected, promoting

Language: Английский

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Genome-wide identification of CCO gene family in cucumber (Cucumis sativus) and its comparative analysis with A. thaliana

BMC Plant Biology, Journal Year: 2023, Volume and Issue: 23(1)

Published: Dec. 11, 2023

Abstract Carotenoid cleavage oxygenase (CCO) is an enzyme capable of converting carotenoids into volatile, aromatic compounds and it plays important role in the production two significant plant hormones, i.e., abscisic acid (ABA) strigolactone (SL). The cucumber genome has not been mined for genomewide identification CCO gene family. In present study, we conducted a comprehensive genome-wide analysis to identify thoroughly examine family within genomic sequence Cucumis sativus L. A Total 10 genes were identified mostly localized cytoplasm chloroplast. divided seven subfamilies i.e. 3 NCED, CCD, 1 CCD-like (CCDL) subfamily according phylogenetic analysis. Cis-regulatory elements (CREs) revealed associated with growth development as well reactions phytohormonal, biotic, abiotic stress conditions. CCOs involved variety physiological metabolic processes, Gene Ontology annotation. Additionally, regulated by 84 miRNA. CsCCO had substantial purifying selection acting upon them, synteny block. addition, RNAseq indicated that expressed response phloem transportation treatment chitosan oligosaccharides. CsCCD7 CsNCED2 showed highest expression exogenous application oligosaccharides improve cold cucumbers. We also found these CsCCD4a CsCCDL-a different organs respect content. was subject first report this which may help us better understand proteins lay groundwork family's future cloning functional investigations.

Language: Английский

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Combined miRNA and mRNA sequencing reveals the defensive strategies of resistant YHY15 rice against differentially virulent brown planthoppers

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: March 18, 2024

Introduction The brown planthopper (BPH) poses a significant threat to rice production in Asia. use of resistant varieties has been effective managing this pest. However, the adaptability BPH led emergence virulent populations, such as biotype Y BPH. YHY15 rice, which carries resistance gene Bph15 , exhibits notable 1 but is susceptible Limited information exists regarding how plants defend against populations with varying levels virulence. Methods In study, we integrated miRNA and mRNA expression profiling analyses study differential responses both avirulent (biotype 1) Y) Results demonstrated rapid response infestation, transcriptional changes occurring within 6 hours. Y-responsive genes were notably enriched photosynthetic processes. Accordingly, infestation induced more intense responses, affecting expression, defenserelated metabolic pathways, phytohormone signaling, multiple transcription factors. Additionally, callose deposition was enhanced BPH-infested seedlings. Discussion These findings provide comprehensive insights into defense mechanisms BPH, may potentially guide development insect-resistant varieties.

Language: Английский

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Bacillus subtilis 26D Triggers Induced Systemic Resistance against Rhopalosiphum padi L. by Regulating the Expression of Genes AGO, DCL and microRNA in Bread Spring Wheat

Microorganisms, Journal Year: 2023, Volume and Issue: 11(12), P. 2983 - 2983

Published: Dec. 14, 2023

Bacillus subtilis 26D is a plant growth-promoting endophytic bacteria capable of inducing systemic resistance through the priming mechanism, which includes genome reprogramming and phenomenon RNA interference (RNAi) microRNA (miRNAs). The phloem-feeding insect bird cherry-oat aphid Rhopalosiphum padi L. serious pest that causes significant damage to crops throughout world. However, function miRNAs in response infestation remains unclear. results this work showed B. stimulated wheat plants, expression genes hormonal signaling pathways ICS, WRKY13, PR1, ACS, EIN3, PR3, ABI5. In addition, activated RNAi mechanism regulated nine conserved activation ethylene, salicylic acid (SA), abscisic (ABA) pathways, was demonstrated by using treatments with phytohormones. Treatment plants SA, ABA acted similar manner on induction AGO4, AGO5 DCL2, DCL4 genes, as well miRNAs. Different patterns miRNA were found aphid-infested treated or infested aphids, suggesting play multiple roles insects, associated effects redox metabolism, synthesis secondary metabolites. Our study provides new data further elucidate fine mechanisms bacterial-induced priming. extensive needed fully unravel these mechanisms.

Language: Английский

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Role of miRNAs in sucrose stress response, reactive oxygen species, and anthocyanin biosynthesis in Arabidopsis thaliana

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 3, 2023

In plants, sucrose is the main transported disaccharide that primary product of photosynthesis and controls a multitude aspects plant life cycle including structure, growth, development, stress response. Sucrose signaling molecule facilitating various adaptations by crosstalk with other hormones, but molecular mechanisms are not well understood. Accumulation high concentrations hallmark many abiotic biotic stresses, resulting in accumulation reactive oxygen species secondary metabolite anthocyanins have antioxidant properties. Previous studies shown several MYeloBlastosis family/MYB transcription factors positive negative regulators sucrose-induced anthocyanin subject to microRNA (miRNA)-mediated post-transcriptional silencing, consistent notion miRNAs may be "nodes" virtue their sequence-guided targeting different homologous family members. this study, we endeavored uncover deep sequencing small RNA mRNA transcriptomes effects exogenous on miRNA abundances validated target transcripts Arabidopsis. We focused genotype-by-treatment Production Anthocyanin Pigment 1-Dominant/pap1-D, an activation-tagged dominant allele MYB75 factor, effector pathway. process, discovered links through miR158/161/173-targeted Pentatrico Peptide Repeat genes two novel non-canonical targets miR408 miR398b*(star), relevant carbon metabolic fluxes: Flavonoid 3'-Hydroxlase (F3'H), important enzyme determining B-ring hydroxylation pattern flavonoids, ORANGE post-translational regulator Phytoene Synthase expression, respectively. Taken together, our results contribute understanding flux shifts from metabolites response sugar stress.

Language: Английский

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