Evolving biofilm inhibition and eradication in clinical settings through plant-based antibiofilm agents

Phytomedicine, Journal Year: 2023, Volume and Issue: 119, P. 154973 - 154973

Published: July 17, 2023

After almost 100 years since evidence of biofilm mode growth and decades intensive investigation about their formation, regulatory pathways mechanisms antimicrobial tolerance, nowadays there are still no therapeutic solutions to eradicate bacterial biofilms biomedical related issues.This review intends provide a comprehensive summary the recent most relevant published studies on plant-based products, or isolated compounds with antibiofilm activity action identified molecular targets against biofilms. The objective is offer new perspective data for clinical researchers aiming prevent eliminate biofilm-associated infections caused by pathogens.The search was performed considering original research articles PubMed, Web Science Scopus from 2015 April 2023, using keywords such as "antibiofilm", "antivirulence", "phytochemicals" "plant extracts".Over 180 were considered this focus priority human pathogens listed World Health Organization, including Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae Escherichia coli. Inhibition detachment dismantling formed these found extract/products derivative compounds. Although combination products antibiotics recorded discussed, topic currently poorly explored only reduced number species.This clearly demonstrates that may be promising strategy associated infections. thoroughly reviewing vast amount carried out over years, it concluded mostly able formation through inhibition quorum sensing signals, but also disrupt mature developed multidrug resistant bacteria targeting extracellular polymeric substance. Flavonoids phenolic seemed effective

Language: Английский

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Carnivorous Plants from Nepenthaceae and Droseraceae as a Source of Secondary Metabolites

Molecules, Journal Year: 2023, Volume and Issue: 28(5), P. 2155 - 2155

Published: Feb. 24, 2023

Carnivorous plants are able to attract small animals or protozoa and retain them in their specialized traps. Later, the captured organisms killed digested. The nutrients contained prey bodies absorbed by use for growth reproduction. These produce many secondary metabolites involved carnivorous syndrome. main purpose of this review was provide an overview family Nepenthaceae Droseraceae, which were studied using modern identification techniques, i.e., high-performance liquid chromatography ultra-high-performance with mass spectrometry nuclear magnetic resonance spectroscopy. After literature screening, there is no doubt that tissues species from genera Nepenthes, Drosera, Dionaea rich sources can be used pharmacy medical purposes. types identified compounds include phenolic acids derivatives (gallic, protocatechuic, chlorogenic, ferulic, p-coumaric acids, gallic, hydroxybenzoic, vanillic, syringic caffeic vanillin), flavonoids (myricetin, quercetin, kaempferol derivatives), including anthocyanins (delphinidin-3-O-glucoside, cyanidin-3-O-glucoside, cyanidin), naphthoquinones (e.g., plumbagin, droserone, 5-O-methyl droserone), volatile organic compounds. Due biological activity most these substances, importance plant as a pharmaceutical crop will increase.

Language: Английский

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Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 4, 2023

Multidrug-resistant gram-negative pathogens such as Escherichia coli have become increasingly difficult to treat and therefore alternative treatment options are needed. Targeting virulence factors like biofilm formation could be one option. Inhibition of biofilm-related structures curli cellulose in E. has been shown for different phenolic natural compounds epigallocatechin gallate. This study demonstrates this effect other structurally unrelated phenolics, namely octyl gallate, scutellarein wedelolactone. To verify whether these influence identical pathways a broad comparative RNA-sequencing approach was chosen with additional RT-qPCR gain initial insights into the affected at transcriptomic level. Bioinformatical analysis RNA-Seq data performed using DESeq2, BioCyc KEGG Mapper. The bioinformatics on revealed that, irrespective their structure, all mainly influenced similar biological processes. These included bacterial motility, chemotaxis, well metabolic processes arginine biosynthesis tricarboxylic acid cycle. Overall, work provides first potential mechanisms action novel inhibitors highlights complex regulatory .

Language: Английский

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Mechanistic Insights into the Antibiofilm Mode of Action of Ellagic Acid

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(6), P. 1757 - 1757

Published: June 17, 2023

Bacterial biofilm is a major contributor to the persistence of infection and limited efficacy antibiotics. Antibiofilm molecules that interfere with lifestyle offer valuable tool in fighting bacterial pathogens. Ellagic acid (EA) natural polyphenol has shown attractive antibiofilm properties. However, its precise mode action remains unknown. Experimental evidence links NADH:quinone oxidoreductase enzyme WrbA formation, stress response, pathogen virulence. Moreover, demonstrated interactions molecules, suggesting role redox modulation. This work aims provide mechanistic insights into EA utilizing computational studies, biophysical measurements, inhibition studies on WrbA, reactive oxygen species assays exploiting WrbA-deprived mutant strain Escherichia coli. Our research efforts led us propose stems from ability perturb homeostasis driven by WrbA. These findings shed new light properties could lead development more effective treatments for biofilm-related infections.

Language: Английский

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Biological Potential of Carnivorous Plants from Nepenthales

Molecules, Journal Year: 2023, Volume and Issue: 28(8), P. 3639 - 3639

Published: April 21, 2023

Since Charles Darwin and his book carnivorous plants have aroused interest heated debate. In addition, there is growing in this group of as a source secondary metabolites the application their biological activity. The aim study was to trace recent literature search extracts obtained from families Droseraceae, Nepenthaceae, Drosophyllaceae show potential. data collected review clearly indicate that studied Nepenthales species great potential terms antibacterial, antifungal, antioxidant, anti-inflammatory, anticancer use. We proposed further investigations should include: (i) bioactivity-guided crude plant extract connect particular type action with specific compound or metabolites; (ii) for new bioactive properties plants; (iii) establishment molecular mechanisms associated Furthermore, research be extended include less explored species, i.e., Drosophyllum lusitanicum especially Aldrovanda vesiculosa.

Language: Английский

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A Targeted Approach for the Metabolome Analysis of E. coli Biofilms

Helvetica Chimica Acta, Journal Year: 2024, Volume and Issue: 107(6)

Published: March 9, 2024

Abstract Biofilms of pathogenic bacteria are responsible for persistent infections in humans, therefore investigations biofilm formation and treatment strategies required. The gram‐negative enterobacterium Escherichia (E.) coli is the most common pathogen causing chronic or recurring urinary tract infections. Metabolomics approaches targeted bacterium to investigate specific metabolic patterns biofilms regulatory influences on formation. In this study, we aimed metabolome formed by multidrug‐resistant extended‐spectrum beta‐lactamase‐producing (ESBL) E. PBIO729. For purpose, a protocol fast sampling macrocolony efficient extraction metabolites was optimized. Validation an LC‐MS/MS method confirmed its usability analysis nucleotides other phosphorylated metabolites. A GC‐MS approach used monitor nutrient uptake from medium addition amino acid content glycolysis TCA cycle biofilms.

Language: Английский

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Individual architecture and photosynthetic performance of the submerged form of Drosera intermedia Hayne

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: May 23, 2024

Abstract Drosera intermedia grows in acidic bogs parts of valleys that are flooded winter, and often dry out summer. It is also described as the sundew most heavily hydrated habitats peatlands, it found water even underwater. This only one can tolerate long periods submersion, more importantly produces a typical submerged form live such conditions for many years. Submerged occupied by D. relatively frequently. The aim study was to determine environmental architecture individuals . features morphological anatomical structure chlorophyll fluorescence this were measured compared with analogous ones occurred emerged peatland habitats. depth 20 cm. Compared other forms, its habitat had highest pH (4.71–4.92; Me = 4.71), temperature substrate hydration, above all, lowest photosynthetically active radiation (PAR; 20.4–59.4%). differed from forms almost all plant’s architecture. particularly noteworthy largest main axis height among which exceeded 18 number living leaves rosette notable (18.1 ± 8.1), while dead very low (6.9 3.8). significant differences shape leaves, length leaf blade (0.493 0.15 mm; p < 0.001) usually widest. stem cross-sectional area noticeably smaller than xylem less developed collaterally closed vascular bundles occurred. Our analysis parameters vivo revealed maximum quantum yield primary photochemistry photosystem II (Me 0.681), same electron transport φE 0 0.183). efficiency energy use per reaction center (RC) 2.978), fraction trapped RC 1.976) non-photochemical dissipation (DI /RC; 0.916). ET /RC parameter, associated utilization RC, plant reached value 0.489). clearly plants thinner however, their stems much longer. high photosynthetic suggests utilized drive photosynthesis minimum loss, may be mechanism compensate small size blade.

Language: Английский

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Effect of Agitation and Temporary Immersion on Growth and Synthesis of Antibacterial Phenolic Compounds in Genus Drosera

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1132 - 1132

Published: Sept. 7, 2024

Sundews (Drosera sp.) are the source of biologically active secondary metabolites: phenolic acids, flavonoids, and 1,4-naphtoquinones. Because obtaining them from natural environment is impossible (rare endangered species), in this study modifications traditional tissue cultures grown solid medium (SM), such as agitated (ACs) (cultures liquid with rotary shaking) temporary immersion bioreactors PlantformTM (TIB), were used for multiplication four sundew species: Drosera peltata, indica, regia, binata, simultaneously effective synthesis compounds. Each species cultivated on SM, AC, TIB was tested biomass accumulation, content total phenols selected derivative concentrations (DAD-HPLC), productivity compounds, well its antibacterial activity against two human pathogens: Staphylococcus aureus Escherichia coli. The results showed that type culture should be each separately. Phytochemical analyses metabolites groups 1,4-naphthoquinones can increased by modifying cultivation conditions. D. regia turned out to richest including 1,4-naphtoquinones: plumbagin ramentaceone. Extracts indica strong both pathogens. It has also been shown growth conditions sundews modify level metabolites, thus, their biological activity.

Language: Английский

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Quality parameters for the medicinal plant Drosera rotundifolia L.: A new approach with established techniques

Archiv der Pharmazie, Journal Year: 2023, Volume and Issue: 357(1)

Published: Nov. 3, 2023

Abstract Monographs of the European Pharmacopoeia (Ph. Eur.) are basis for quality control medicinal plants and therefore important to ensure consistency, quality, safety, efficacy phytopharmaceuticals. The traditional plant sundew ( Drosera sp.) has disappeared from therapy due nature conservation, but can now be cultivated sustainably on rewetted peatland. However, currently there is no valid Ph. Eur. monograph Droserae herba. In this study, material different species sources was investigated with aim developing methods based defining a uniform standard It possible distinguish between using macroscopic, microscopic, chromatographic methods. Special emphasis laid content flavonoids naphthoquinones as parameters their differed species. differences in toxicity result recommendation that only round‐leaved rotundifolia L.) should used production phytopharmaceuticals future.

Language: Английский

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Nanotechnology in food safety

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 159 - 189

Published: Nov. 29, 2024

Language: Английский

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