Bioactive compounds by microalgae and potentials for the management of some human disease conditions

AIMS Microbiology, Journal Year: 2023, Volume and Issue: 9(1), P. 55 - 74

Published: Jan. 1, 2023

Microalgae biomasses are excellent sources of diverse bioactive compounds such as lipids, polysaccharides, carotenoids, vitamins, phenolics and phycobiliproteins. Large-scale production these substances would require microalgae cultivation either in open-culture systems or closed-culture systems. Some (such phycobiliproteins lipids) produced during their active growth phase. They appear to have antibacterial, antifungal, antiviral, antioxidative, anticancer, neuroprotective chemo-preventive activities. These properties confer on the potential for use treatment and/or management several neurologic cell dysfunction-related disease conditions, including Alzheimer's (AD), AIDS COVID-19, shown this review. Although health benefits been highlighted, there appears be a consensus literature that field is still fledgling, more research needs carried out ascertain mechanisms action underpin effectiveness microalgal compounds. In review, two biosynthetic pathways were modeled help elucidate mode from products. carotenoid phycobilin proteins pathways. The education public importance backed with empirical scientific evidence will go long way ensure investigations quickly rolled out. application some human conditions was highlighted.

Language: Английский

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Recent advances and fundamentals of microalgae cultivation technology

Biotechnology Journal, Journal Year: 2024, Volume and Issue: 19(3)

Published: March 1, 2024

Abstract Microalgae are considered to be a promising group of organisms for fuel production, waste processing, pharmaceutical applications, and as source food components. Unicellular algae worth being because their capacity produce comparatively large amounts lipids, proteins, vitamins while requiring little room growth. They can also grow on fix CO2 nitrogen compounds. However, production costs limit the industrial use microalgae most profitable applications including micronutrient fish farming. Therefore, novel based technologies require an increase efficiencies or values. Here we review recent studies focused getting strains with characteristics cultivating techniques that improve production's robustness efficiency categorize these findings according fundamental factors determine Improvements light nutrient delivery, well other aspects photobioreactor design, have shown highest average in productivity. Other methods, such improvement phosphorus fixation temperature adaptation been found less effective. Furthermore, interactions particular bacteria may promote growth microalgae, although bacterial grazer contaminations must managed avoid culture failure. The competitiveness algal products will if discoveries applied settings.

Language: Английский

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Biomanufacturing of glycosylated antibodies: Challenges, solutions, and future prospects

Biotechnology Advances, Journal Year: 2023, Volume and Issue: 69, P. 108267 - 108267

Published: Oct. 7, 2023

Language: Английский

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Potential of Microalgae as Functional Foods Applied to Mitochondria Protection and Healthy Aging Promotion

Nutraceuticals, Journal Year: 2023, Volume and Issue: 3(1), P. 119 - 152

Published: Feb. 6, 2023

The rapid aging of the Western countries’ populations makes increasingly necessary promotion healthy lifestyles in order to prevent/delay onset age-related diseases. use functional foods can significantly help achieve this aim, thanks contribution biologically active compounds suitable protect cellular and metabolic homeostasis from damage caused by stress factors. Indeed, excessive production reactive oxygen species (ROS), favored incorrect eating behavioral habits, are considered causal elements oxidative stress, which turn favors tissue organism aging. Microalgae represent a convenient food because their extraordinary ability concentrate various compounds, comprising omega-3 polyunsaturated fatty acids, sterols, phenolic carotenoids others. Within cells, mitochondria organelles most affected accumulation molecular produced stress. Since, addition producing chemical energy for metabolism, control numerous cell cycle regulation processes, including intrinsic apoptosis, responses inflammatory signals other biochemical pathways, dysfunction is decisive many pathologies. Among these, some degenerative diseases nervous system, cardiovascular kidney function even cancer found. From viewpoint, bioactive microalgae, possessing high antioxidant properties, enhance mitochondrial functionality modulating expression protective factors enzymes, regulate essential pathways preservation integrity cell. Here, we summarize current knowledge on role played microalgal life cycle, reparative apoptosis modulation key pathways. Special attention was paid composition cultivable microalgae strains selected content improve functions.

Language: Английский

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Progress in oral biomaterials for the treatment of metabolic bone diseases

Progress in Materials Science, Journal Year: 2025, Volume and Issue: unknown, P. 101499 - 101499

Published: April 1, 2025

Language: Английский

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Chlamydomonas reinhardtii—A Reference Microorganism for Eukaryotic Molybdenum Metabolism

Microorganisms, Journal Year: 2023, Volume and Issue: 11(7), P. 1671 - 1671

Published: June 27, 2023

Molybdenum (Mo) is vital for the activity of a small but essential group enzymes called molybdoenzymes. So far, specifically five molybdoenzymes have been discovered in eukaryotes: nitrate reductase, sulfite oxidase, xanthine dehydrogenase, aldehyde and mARC. In order to become biologically active, Mo must be chelated pterin, forming so-called cofactor (Moco). Deficiency or mutation any genes involved Moco biosynthesis results simultaneous loss all molybdoenzymes, fully partially preventing normal development affected organism. To prevent this, different mechanisms homeostasis finely regulated. Chlamydomonas reinhardtii unicellular, photosynthetic, eukaryotic microalga that has produced fundamental advances key steps over last 30 years, which extrapolated higher organisms, both plants animals. These include identification first two molybdate transporters eukaryotes (MOT1 MOT2), characterization biosynthesis, enzyme protects transfers (MCP1), mARC plants, discovery crucial role reductase–mARC complex plant nitric oxide production. This review aims provide comprehensive summary progress achieved using C. as model organism propose how this can continue improving with advancements field future.

Language: Английский

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A Novel Probiotic-Based Oral Vaccine against SARS-CoV-2 Omicron Variant B.1.1.529

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(18), P. 13931 - 13931

Published: Sept. 11, 2023

COVID-19 pandemic, caused by the SARS-CoV-2 virus, is still affecting entire world via rapid emergence of new contagious variants. Vaccination remains most effective prevention strategy for viral infection, yet not all countries have sufficient access to vaccines due limitations in manufacturing and transportation. Thus, there an urgent need develop easy-to-use, safe, low-cost vaccination approach. Genetically modified microorganisms, especially probiotics, are now commonly recognized as attractive vehicles delivering bioactive molecules oral mucosal routes. In this study, Lactobacillus casei has been selected vaccine candidate based on its’ natural immunoadjuvant properties ability resist acidic gastric environment, express antigens Omicron variant B.1.1.529 with B-cell T-cell epitopes. This newly developed vaccine, OMGVac, was shown elicit a robust IgG systemic immune response against spike protein Golden Syrian hamsters. No adverse effects were found throughout overall safety evaluated terms physiological histopathological examinations different organs harvested. addition, study illustrated use recombinant probiotic live delivery vector initiation immunity, which shed light future development next-generation combat emerging infectious diseases.

Language: Английский

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Recombinant Protein Expression and Its Biotechnological Applications in Chlorella spp.

SynBio, Journal Year: 2024, Volume and Issue: 2(2), P. 223 - 239

Published: June 6, 2024

Recombinant protein expression is a fundamental aspect of both synthetic biology and biotechnology as well field unto itself. Microalgae, with their eukaryotic cellular machinery, high lipid content, cost-effective cultivation conditions, safety profile for human consumption, environmentally friendly attributes, are promising system or metabolic engineering sustainable chemical production. Amongst the incredible diversity microalgae species, Chlorella spp. heavily studied due to growth efficiency, potential low-cost cultivation, well-characterized scale-up process large-scale cultivation. This review aims comprehensively examine ongoing advancements in bioengineering recombinant production its biotechnological applications. includes genetic elements such promoters, terminators, reporters markers, enhancers, tags successfully used

Language: Английский

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Comparative RNA-Seq of Ten Phaeodactylum tricornutum Accessions: Unravelling Criteria for Robust Strain Selection from a Bioproduction Point of View

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(8), P. 353 - 353

Published: July 30, 2024

The production of biologics in mammalian cells is hindered by some limitations including high costs, prompting the exploration other alternative expression systems that are cheaper and sustainable like microalgae. Successful productions such as monoclonal antibodies have already been demonstrated diatom Phaeodactylum tricornutum; however, limited yields still remain compared to cells. Therefore, efforts needed make this microalga more competitive a cell biofactory. Among seventeen reported accessions P. tricornutum, ten mainly studied so far. them, used produce high-value-added molecules biologics. use “omics” increasingly being described useful for improvement both upstream downstream steps bioprocesses using context, we performed an RNA-Seq analysis most tricornutum (Pt1 Pt10) deciphered differential gene pathways could affect bioproduction tricornutum. Our results highlighted benefits certain Pt9 or Pt4 Indeed, these seem be advantageous. Moreover, contribute better understanding molecular cellular biology

Language: Английский

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Chlamydomonas reinhardtii a Reference Microorganism for Eukaryotic Molybdenum Homeostasis

Published: May 18, 2023

Molybdenum (Mo) is vital for the activity of a small but essential group enzymes called mo-lybdoenzymes. So far, specifically five molybdoenzymes have been discovered in eukaryotes: nitrate reductase, sulfite oxidase, xanthine dehydrogenase, aldehyde and mARC. In order to become biologically active, Mo must be chelated pterin, forming so-called cofactor (Moco). Deficiency or mutation any genes involved Moco biosynthesis results simultaneous loss all molybdoenzymes, fully partially preventing normal development affected organism. To prevent this, different mechanisms in-volved homeostasis finely regulated. Chlamydomonas reinhardtii unicellular, photosynthetic, eukaryotic microalga that has produced fundamental advances key steps over last 30 years, which extrapolated higher organisms, both plants animals. These include identification first two molybdate transporters eukaryotes (MOT1 MOT2), characterization biosynthesis, enzyme protects transfers (MCP1), characteriza-tion mARC plants, discovery crucial role reductase-mARC complex plant nitric oxide production. This review aims summarize these made using C. as model organism suggest how this can continue contribute progress field.

Language: Английский

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