Innovative approaches for enzyme immobilization in milk processing: advancements and industrial applications DOI

Mati Ullah Khan,

A. Farid,

Shuang Liu

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 20

Published: Jan. 22, 2025

The dairy industry is progressively integrating advanced enzyme technologies to optimize processing efficiency and elevate product quality. Among these technologies, immobilization has emerged as a pivotal innovation, offering considerable benefits in terms of reusability, stability, overall process sustainability. This review paper explores the latest improvements techniques their industrial applications within milk processing. It examines various strategies, including adsorption, affinity binding, ionic covalent entrapment, encapsulation, cross-linking, highlighting effectiveness improving performance key enzymes such lactases, lipases, proteases transglutaminases. also delves into economic ecological immobilization, emphasizing its role reducing production costs environmental impact while maintaining or enhancing quality products. By analyzing current trends technological developments, this provides comprehensive overview how innovative approaches are transforming concludes with discussion on future research directions potential applications, underscoring importance continued innovation field meet increasing demands global market.

Language: Английский

Nano-based biofuel production from low-cost lignocellulose biomass: environmental sustainability and economic approach DOI
Selvakumar Sakthivel,

Kanthimathi Muthusamy,

Amutha Priya Thangarajan

et al.

Bioprocess and Biosystems Engineering, Journal Year: 2024, Volume and Issue: 47(7), P. 971 - 990

Published: March 30, 2024

Language: Английский

Citations

7
Integration of microfluidic chips with biosensors DOI Creative Commons

Masindi Sekhwama,

Kelvin Mpofu,

Sivarasu Sudesh

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(9)

Published: Aug. 23, 2024

Abstract The potential threat posed by disease outbreaks to diagnostic instruments demands the development of more effective biosensor technologies counteract risks. Diseases like SARS-CoV-2, Ebola, malaria, cholera, and many have demonstrated beyond limits health care that new advancements are required for early detection diagnosis. rising number diseases has led an increasing demand biosensors quicker utilize in healthcare settings. A incorporated with microfluidic chips offers improved compared traditional or classical biosensors. Microfluidic improve performance allowing automation, mixing, separation, throughput processing, transport analytes desired reactors. a chip sensitivity, easy operation can use small volumes samples process results. effectiveness depends also on materials used its fabrication there which reviewed this work. This paper reviews advantages enhance biosensors, fabricate chips, electrodes into time shortening processing at point service. work not previously addressed other including, integration cell-imprinted polymers sensors delved future outlook.

Language: Английский

Citations

7
Green nanobiocatalysts: enhancing enzyme immobilization for industrial and biomedical applications DOI Creative Commons
Doaa S. R. Khafaga, Ghazala Muteeb, Abdullrahman Elgarawany

et al.

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17589 - e17589

Published: July 8, 2024

Nanobiocatalysts (NBCs), which merge enzymes with nanomaterials, provide a potent method for improving enzyme durability, efficiency, and recyclability. This review highlights the use of eco-friendly synthesis methods to create sustainable nanomaterials transport. We investigate different immobilization, such as adsorption, ionic covalent bonding, entrapment, cross-linking, examining their pros cons. The decreased environmental impact green-synthesized from plants, bacteria, fungi is emphasized. exhibits various uses NBCs in food industry, biofuel production, bioremediation, showing how they can enhance effectiveness eco-friendliness. Furthermore, we explore potential biomedicine. In general, green nanobiocatalysts are notable progression technology, leading environmentally-friendly effective biocatalytic that have important impacts on industrial biomedical fields.

Language: Английский

Citations

6
Enhanced Biodiesel Production with Eversa Transform 2.0 Lipase on Magnetic Nanoparticles DOI Creative Commons
Kaiany Moreira dos Santos,

Juliana de França Serpa,

Viviane de Castro Bizerra

et al.

Langmuir, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

This research investigated the usefulness of magnetic iron oxide nanoparticles (Fe3O4) as a support to immobilize lipase Eversa Transform 2.0 (ET 2.0) obtain an active and stable biocatalyst, easily recoverable from reaction medium for applications in production biodiesel. Biodiesel was alternative fuel composed mainly fatty acid esters with strong transesterification esterification capabilities. The study focused on oleic ethanol synthesize ethyl oleate. Magnetic were prepared by coprecipitation, then activated glutaraldehyde functionalized γ-aminopropyltriethoxysilane (APTES). optimal conditions immobilizing ET pH 10, 25 mM sodium carbonate buffer, enzymatic load 200 U/g, 1 h contact time, obtaining 78% yield activity 205.9 U/g. Postimmobilization evaluation showed that immobilized enzyme performed better than its free form. Kinetic studies conducted under these optimized (2–96 at 150 rpm 37 °C). biocatalyst tested synthesis oleate using substrate ethanol, achieving conversion 88.1%. Subsequent recirculation tests maintained approximately 80% until fourth cycle, confirming sustainability ester production. Molecular docking revealed binding affinity enzyme-docked oil composition estimated −5.8 kcal/mol, suggesting combination suitable esterification.

Language: Английский

Citations

6
Heterofunctional Methacrylate Beads Bearing Octadecyl and Vinyl Sulfone Groups: Tricks to Obtain an Interfacially Activated Lipase from Thermomyces lanuginosus and Covalently Attached to the Support DOI Open Access
José Renato Guimarães, Diego Carballares, Javier Rocha‐Martín

et al.

Catalysts, Journal Year: 2023, Volume and Issue: 13(1), P. 108 - 108

Published: Jan. 3, 2023

Lipase from Thermomyces lanuginosus (TLL) has been immobilized on a methacrylate macroporous resin coated with octadecyl groups (Purolite Lifetech®® ECR8806F). This immobilization protocol gave biocatalyst significantly higher stability than that obtained using octyl agarose. To further improve the features, we tried to covalently immobilize enzyme this support. For purpose, support was activated divinyl sulfone. The results showed at least 1/3 of molecules were not immobilized. solve problem, produced an aminated and then it permitted full covalent previously TLL. use different blocking agents as reaction endpoint (using ethylenediamine, Asp, Gly, Cys) greatly altered functional features (activity, specificity, or stability). example, ethylenediamine increased ratio activity versus R- S-methyl mandelate by three-fold factor. Cys most stable biocatalyst, maintaining close 90% under conditions where just adsorbed maintained less 55%. That way, strategy modify obtaining interfacially layer and, later, vinyl sulfone groups.

Language: Английский

Citations

16
Direct Biocatalytic Processes for CO2 Capture as a Green Tool to Produce Value-Added Chemicals DOI Creative Commons
Rocío Villa, Susana Nieto, Antonio Donaire

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(14), P. 5520 - 5520

Published: July 19, 2023

Direct biocatalytic processes for CO2 capture and transformation in value-added chemicals may be considered a useful tool reducing the concentration of this greenhouse gas atmosphere. Among other enzymes, carbonic anhydrase (CA) formate dehydrogenase (FDH) are two key biocatalysts suitable challenge, facilitating uptake carbon dioxide from atmosphere complementary ways. Carbonic anhydrases accelerate by promoting its solubility water form hydrogen carbonate as first step converting into species widely used storage utilization (CCSU), particularly carbonation mineralization methods. On hand, dehydrogenases represent machinery evolved certain organisms to convert enriched, reduced, easily transportable species, such formic acid, via enzymatic cascade systems that obtain energy chemical electrochemical sources, or light. Formic acid is basis fixing C1-carbon other, more reduced molecules. In review, state-of-the-art both methods assessed, highlighting biotechnological approaches have been developed using enzymes.

Language: Английский

Citations

16
Recent trends in antioxidative peptides derived from soybean and other soy-based products: A comprehensive review DOI

C. C. Kalita,

Unmesh Mehta,

Krishna Aayush

et al.

Process Biochemistry, Journal Year: 2023, Volume and Issue: 136, P. 311 - 323

Published: Dec. 6, 2023

Language: Английский

Citations

13
Enzyme Engineering: Performance Optimization, Novel Sources, and Applications in the Food Industry DOI Creative Commons
Shucan Mao, Jun Jiang, Ke Xiong

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(23), P. 3846 - 3846

Published: Nov. 28, 2024

This review summarizes the latest progress in enzyme preparation, including design and modification technology, exploration of new sources, application preparation food processing, detection, preservation. The directed evolution technology improved stability catalytic efficiency enzymes, while immobilization enhanced reusability industrial applicability. Extremozymes biomimetic enzymes exhibit excellent performance under harsh conditions. In can improve quality flavor. combined with immune detection biosensors realize rapid allergens, pollutants, pesticide residues. preservation, enhance by extending shelf life inhibiting microbial growth. future, engineering will be computer-aided design, artificial intelligence, material to promote intelligent multifunctional development help technological upgrading sustainable industry green chemistry.

Language: Английский

Citations

5
A Review on Flexible Electrochemical Biosensors to Monitor Alcohol in Sweat DOI Creative Commons
Nuna G. Costa, Joana C. Antunes, Antonio J. Paleo

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 12(4), P. 252 - 252

Published: April 16, 2022

The continued focus on improving the quality of human life has encouraged development increasingly efficient, durable, and cost-effective products in healthcare. Over last decade, there been substantial field technical interactive textiles that combine expertise electronics, biology, chemistry, physics. Most recently, creation textile biosensors capable quantifying biometric data biological fluids is being studied, to detect a specific disease or physical condition an individual. ultimate goal provide access medical diagnosis anytime anywhere. Presently, alcohol considered most commonly used addictive substance worldwide, one main causes death road accidents. Thus, it important think solutions minimizing this public health problem. Alcohol constitute excellent tool aid at safety. Hence, review explores concepts about biomarkers, composition sweat correlation between blood. Different components requirements biosensor are reviewed, along with electrochemical techniques evaluate its performance, addition construction textile-based biosensors. Special attention given determination biomarkers must be low cost fast, so use biomimetic materials recognize target analyte turning into attractive option improve behavior.

Language: Английский

Citations

22
Multienzyme Coimmobilization on Triheterofunctional Supports DOI Creative Commons
Javier Santiago‐Arcos, Susana Velasco‐Lozano, Fernándo López-Gallego

et al.

Biomacromolecules, Journal Year: 2023, Volume and Issue: 24(2), P. 929 - 942

Published: Jan. 17, 2023

Immobilized multienzyme systems are gaining momentum in applied biocatalysis; however, the coimmobilization of several enzymes on one carrier is still challenging. In this work, we exploited a heterofunctional support activated with three different chemical functionalities to immobilize wide variety enzymes. This based agarose microbeads aldehyde, amino, and cobalt chelate moieties that allow fast irreversible immobilization enzymes, enhancing thermostability most heterogeneous biocatalysts (up 21-fold higher than soluble one). Furthermore, trifunctional serves efficiently coimmobilize system composed an alcohol dehydrogenase, reduced nicotinamide adenine dinucleotide (NADH) oxidase, catalase. The confined multienzymatic demonstrates performance its free counterpart, achieving total turnover number (TTN) 1 × 105 during five batch consecutive cycles. We envision solid material as platform for coimmobilizing enhanced properties catalyze stepwise biotransformations.

Language: Английский

Citations

12