Electroacupuncture Attenuates Intestinal Barrier Disruption via the α7nAChR-Mediated HO-1/p38 MAPK/NF-κB Pathway in a Mouse Model of Metabolic Dysfunction-Associated Fatty Liver Disease: A Randomized, Single-Blind, Controlled Trial

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 802 - 802

Published: March 27, 2025

Background: Gut barrier integrity plays a crucial role in the pathogenesis of metabolic dysfunction-associated fatty liver disease (MAFLD). Electroacupuncture (EA) at ST-36 can ameliorate inflammatory responses via stimulating α7 nicotinic acetylcholine receptor (α7nAChR), but whether EA is effective preserving intestinal MAFLD has not been exactly illustrated. This investigation explored potential protection mechanisms targeting dismantled gut MAFLD. Methods: C57BL/6 mice were randomly allocated into several subgroups: control (CON), high-fat diet (HFD), HFD with EA, and α7nAChR inhibitor α-BGT, HO-1 knockout (KO). Body weight, visceral fat index, histopathological examination intestine determined. Serum biological indexes evaluated through corresponding kits. Furthermore, expressions HO-1, α7nAChR, barrier-associated proteins, molecular tissues assessed Western blot, RT-qPCR, immunohistology, or immunofluorescence examination. Results: treatment decreased body index gain mitigated function injury abnormal lipid indexes, exhibiting less severity hepatic steatosis, fibrosis, inflammation Lower permeability, epithelial disruption, upregulation tight junction proteins after suggested protective effects attenuating dysfunction. These abolished by α-BGT deletion. Mechanistically, markedly enriched expression phosphorylated p38 MAPK/NF-κB activation, which was lost KO treatment. Conclusions: The may be attributed to preserved barrier, thereby alleviating systemic preventing subsequent hits, where α7nAChR-mediated HO-1/p38 pathway maintain homeostasis.

Language: Английский

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New insights on metabolic reprogramming in macrophage plasticity

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 157, P. 114797 - 114797

Published: May 7, 2025

Language: Английский

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PA suppresses antitumor immunity of T cells by disturbing mitochondrial activity through Akt/mTOR-mediated Ca2+ flux

Cancer Letters, Journal Year: 2023, Volume and Issue: 581, P. 216511 - 216511

Published: Nov. 25, 2023

Language: Английский

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Crosstalk between hypoxia-inducible factor-1α and short-chain fatty acids in inflammatory bowel disease: key clues toward unraveling the mystery

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 28, 2024

The human intestinal tract constitutes a complex ecosystem, made up of countless gut microbiota, metabolites, and immune cells, with hypoxia being fundamental environmental characteristic this ecology. Under normal physiological conditions, delicate balance exists among these “residents”, disruptions potentially leading to inflammatory bowel disease (IBD). core pathology IBD features disrupted epithelial barrier, alongside evident microecological disturbances. Central interconnected networks is hypoxia-inducible factor-1α (HIF-1α), which key regulator in cells for adapting hypoxic conditions maintaining homeostasis. Short-chain fatty acids (SCFAs), as pivotal serve vital mediators between the host significantly influence ecosystem. Recent years have seen surge research on roles therapeutic potential HIF-1α SCFAs independently, yet reviews HIF-1α-mediated regulation under are scarce. This article summarizes evidence interplay regulatory relationship IBD, elucidating disease’s pathogenesis offering promising strategies.

Language: Английский

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Efficacy and Safety of Habitual Consumption of a Food Supplement Containing Miraculin in Malnourished Cancer Patients: the CLINMIR Pilot Study

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 16, 2024

Abstract Taste disorders (TDs) are common among systemically treated cancer patients and negatively impact their nutritional status quality of life. A food supplement containing the natural taste-modifying protein miraculin (DMB ® ) has emerged as a possible alternative treatment for TDs. The present study aimed to evaluate efficacy safety habitual DMB consumption in malnourished undergoing active treatment. An exploratory clinical trial was carried out which 31 were randomized into three arms [standard dose (150 mg DMB/tablet), high (300 DMB/tablet) or placebo freeze-dried strawberry)] months. Patients consumed an intervention tablet before each main meal. Throughout five visits, electrochemical taste perception, status, dietary intake, life fatty acid profile erythrocytes evaluated. consuming standard exhibited improved acuity over time (% change right/left side: ‒52.8 ± 38.5 / ‒58.7 69.2%) salty perception (2.29 1.25 vs. dose: 2.17 1.84 placebo: 1.57 1.51 points, p < 0.05). They also had higher energy intake (p = 0.075) covered better expenditure (107 19%). evaluated by symptom scales receiving (constipation, 0.048). levels arachidonic (13.1 1.8; 14.0 2.8, 12.0 2.0%; 0.004) do-cosahexaenoic (4.4 1.7; 4.1 1.0; 3.9 1.6%; 0.014) acids increased after intake. fat‒free mass vs . (47.4 9.3 44.1 4.7 kg, 0.007). Importantly, with did not experience any adverse events, metabolic parameters remained stable within normal ranges. In conclusion, 150 improves (energy fat quantity quality, fat-free mass) antineoplastic Additionally, appears be safe, no changes major biochemical associated health status. registered at http://clinicaltrials.gov ( NCT05486260 ).

Language: Английский

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Inflammasomes Are Influenced by Epigenetic and Autophagy Mechanisms in Colorectal Cancer Signaling

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 6167 - 6167

Published: June 3, 2024

Inflammasomes contribute to colorectal cancer signaling by primarily inducing inflammation in the surrounding tumor microenvironment. Its role is receiving increasing attention, as has a protumor effect addition tissue damage. The inflammasome's function complex and controlled several layers of regulation. Epigenetic processes impact functioning or manifestation genes that are involved control inflammasomes subsequent cascades. Researchers have intensively studied significance epigenetic mechanisms regulation, they encompass potential therapeutic targets. regulatory interactions between inflammasome autophagy intricate, exhibiting both advantageous harmful consequences. aspects two entities also relationship activation inflammasome, autophagy, alterations CRC involves interrelated pathways. This article provides brief summary newest studies on how epigenetics with special focus their cancer. Based latest findings, we provide an overview ideas for this network.

Language: Английский

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Efficacy and Safety of Habitual Consumption of a Food Supplement Containing Miraculin in Malnourished Cancer Patients: The CLINMIR Pilot Study

Nutrients, Journal Year: 2024, Volume and Issue: 16(12), P. 1905 - 1905

Published: June 17, 2024

Taste disorders (TDs) are common among systemically treated cancer patients and negatively impact their nutritional status quality of life. The novel food approved by the European Commission (EFSA), dried miracle berries (DMB), contains natural taste-modifying protein miraculin. DMB, also available as a supplement, has emerged possible alternative treatment for TDs. present study aimed to evaluate efficacy safety habitual DMB consumption in malnourished undergoing active treatment. An exploratory clinical trial was carried out which 31 were randomized into three arms [standard dose (150 mg DMB/tablet), high (300 DMB/tablet) or placebo freeze-dried strawberry)] months. Patients consumed tablet daily before each main meal (breakfast, lunch, dinner). Throughout five visits, electrochemical taste perception, status, dietary intake, life fatty acid profile erythrocytes evaluated. consuming standard exhibited improved acuity over time (% change right/left side: −52.8 ± 38.5/−58.7 69.2%) salty perception (2.29 1.25 vs. dose: 2.17 1.84 placebo: 1.57 1.51 points, p < 0.05). They had higher energy intake (p = 0.075) covered better expenditure (107 19%). evaluated symptom scales receiving (constipation, 0.048). levels arachidonic (13.1 1.8; 14.0 2.8, 12.0 2.0%; 0.004) docosahexaenoic (4.4 1.7; 4.1 1.0; 3.9 1.6%; 0.014) acids increased after intake. fat-free mass (47.4 9.3 44.1 4.7 kg, 0.007). Importantly, with did not experience any adverse events, metabolic parameters remained stable within normal ranges. In conclusion, 150 improves (energy fat quantity quality, mass), antineoplastic Additionally, appears be safe, no changes major biochemical associated health status. Clinical registered (NCT05486260).

Language: Английский

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Investigating the Impact of Diet, Supplementation, Microbiota, Gut-Brain Axis in Schizophrenia: A Narrative Review

Published: June 25, 2024

Schizophrenia is a disease with complex etiology that significantly impairs the functioning of patients. In recent years, there has been increasing focus on importance gut microbiota in context gut-brain axis. our study, we analyzed data axis schizophrenia, as well impact eating habits, use various supplements, and diets schizophrenia. Additionally, study investigated antipsychotics development metabolic disorders such diabetes, dyslipidemia, obesity. There may be significant clinical benefits to gained from therapies supported by supplements omega-3 fatty acids, B vitamins, probiotics. The results suggest need for holistic approach treatment incorporating both drug therapy dietary interventions.

Language: Английский

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Fecal microbiome transfer an investigative tool and treatment strategy in cancer

AJP Gastrointestinal and Liver Physiology, Journal Year: 2024, Volume and Issue: 327(5), P. G711 - G726

Published: Oct. 8, 2024

The microbiome impacts the onset, progression, and therapy response of certain types cancer. Fecal microbial transplants (FMTs) are an increasingly prevalent tool to test these mechanisms that require standardization by field.

Language: Английский

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Psidium guajava Seed Oil Reduces the Severity of Colitis Induced by Dextran Sulfate Sodium by Modulating the Intestinal Microbiota and Restoring the Intestinal Barrier

Foods, Journal Year: 2024, Volume and Issue: 13(17), P. 2668 - 2668

Published: Aug. 24, 2024

The oil derived from

Language: Английский

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