Sexual arousal gates visual processing during Drosophila courtship

Nature, Journal Year: 2021, Volume and Issue: 595(7868), P. 549 - 553

Published: July 7, 2021

Language: Английский

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Transforming representations of movement from body- to world-centric space

Nature, Journal Year: 2021, Volume and Issue: 601(7891), P. 98 - 104

Published: Dec. 15, 2021

Language: Английский

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Whole-brain annotation and multi-connectome cell typing of Drosophila

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 139 - 152

Published: Oct. 2, 2024

Language: Английский

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A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Feb. 23, 2023

Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of

Language: Английский

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Whole-brain annotation and multi-connectome cell typing quantifies circuit stereotypy inDrosophila

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 27, 2023

Abstract The fruit fly Drosophila melanogaster combines surprisingly sophisticated behaviour with a highly tractable nervous system. A large part of the fly’s success as model organism in modern neuroscience stems from concentration collaboratively generated molecular genetic and digital resources. As presented our FlyWire companion paper 1 , this now includes first full brain connectome an adult animal. Here we report systematic hierarchical annotation ∼130,000-neuron including neuronal classes, cell types developmental units (hemilineages). This enables any researcher to navigate huge dataset find systems neurons interest, linked literature through Virtual Fly Brain database 2 . Crucially, resource 4,552 types. 3,094 are rigorous consensus validations previously proposed “hemibrain” 3 In addition, propose 1,458 new types, arising mostly fact that spans whole brain, whereas hemibrain derives subvolume. Comparison showed type counts strong connections were largely stable, but connection weights variable within across animals. Further analysis defined simple heuristics for interpretation: stronger than 10 unitary synapses or providing >1% input target conserved. Some increased variability connectomes: most common mushroom body, required learning memory, is almost twice numerous hemibrain. We evidence functional homeostasis adjustments absolute amount excitatory while maintaining excitation-inhibition ratio. Finally, surprisingly, about one third could not yet be reliably identified connectome. therefore suggest should robust inter-individual variation, namely groups cells quantitatively more similar different other same brain. Joint connectomes demonstrates viability utility definition. Our work defines atlas provides both intellectual framework open source toolchain brain-scale comparative connectomics.

Language: Английский

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Ascending neurons convey behavioral state to integrative sensory and action selection brain regions

Nature Neuroscience, Journal Year: 2023, Volume and Issue: 26(4), P. 682 - 695

Published: March 23, 2023

Knowing one's own behavioral state has long been theorized as critical for contextualizing dynamic sensory cues and identifying appropriate future behaviors. Ascending neurons (ANs) in the motor system that project to brain are well positioned provide such signals. However, what ANs encode where they convey these signals remains largely unknown. Here, through large-scale functional imaging behaving animals morphological quantification, we report encoding targeting of hundreds genetically identifiable adult fly, Drosophila melanogaster. We reveal states, specifically conveying self-motion anterior ventrolateral protocerebrum, an integrative hub, discrete actions gnathal ganglia, a locus action selection. Additionally, AN projection patterns within predictive their encoding. Thus, ascending populations poised inform distinct hubs ongoing behaviors may important substrate computations required adaptive behavior.

Language: Английский

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Mapping the neural dynamics of locomotion across the Drosophila brain

Current Biology, Journal Year: 2024, Volume and Issue: 34(4), P. 710 - 726.e4

Published: Jan. 21, 2024

Language: Английский

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NeuronBridge: an intuitive web application for neuronal morphology search across large data sets

BMC Bioinformatics, Journal Year: 2024, Volume and Issue: 25(1)

Published: March 15, 2024

Abstract Background Neuroscience research in Drosophila is benefiting from large-scale connectomics efforts using electron microscopy (EM) to reveal all the neurons a brain and their connections. To exploit this knowledge base, researchers relate connectome’s structure neuronal function, often by studying individual neuron cell types. Vast libraries of fly driver lines expressing fluorescent reporter genes sets have been created imaged confocal light (LM), enabling targeting for experimentation. However, creating line driving gene expression within single found an EM connectome remains challenge, as it typically requires identifying pair where only interest expressed both. This task other emerging scientific workflows require finding similar across large data different modalities. Results Here, we present NeuronBridge, web application easily rapidly putative morphological matches between We describe functionality construction NeuronBridge service, including its user-friendly graphical user interface (GUI), extensible model, serverless cloud architecture, massively parallel image search engine. Conclusions fills critical gap workflow used hundreds neuroscience around world. offer our software code, open APIs, processed integration reuse, provide service at http://neuronbridge.janelia.org .

Language: Английский

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Transforming descending input into behavior: The organization of premotor circuits in the Drosophila Male Adult Nerve Cord connectome

Published: March 18, 2024

In most animals, a relatively small number of descending neurons (DNs) connect higher brain centers in the animal’s head to circuits and motor (MNs) nerve cord body that effect movement limbs. To understand how signals generate behavior, it is critical these pathways are organized onto MNs. fly, Drosophila melanogaster , MNs controlling muscles leg, wing, other systems reside ventral (VNC), analogous mammalian spinal cord. companion papers, we introduced densely-reconstructed connectome Male Adult Nerve Cord (MANC, Takemura et al., 2023), including cell type developmental lineage annotation (Marin which provides complete VNC connectivity at synaptic resolution. Here, present first look organization networks connecting DNs based on this new information. We proofread curated all ensure accuracy reliability, then systematically matched DN axon terminals MN dendrites with light microscopy data link their morphology inputs or muscle targets. report both broad organizational patterns entire network fine-scale analysis selected interest. discover direct DN-MN connections infrequent identify communities intrinsic linked control different systems, putative for walking, dorsal flight steering power generation, intermediate lower tectulum coordinated action wings legs. Our generates hypotheses future functional experiments and, together MANC connectome, empowers others investigate richer mechanistic detail.

Language: Английский

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Nested neural circuits generate distinct acoustic signals during Drosophila courtship

Current Biology, Journal Year: 2024, Volume and Issue: 34(4), P. 808 - 824.e6

Published: Jan. 30, 2024

Many motor control systems generate multiple movements using a common set of muscles. How are premotor circuits able to flexibly diverse movement patterns? Here, we characterize the neuronal that drive distinct courtship songs Drosophila melanogaster. Male flies vibrate their wings toward females produce two different song modes—pulse and sine song—which signal species identity male quality. Using cell-type-specific genetic reagents connectome, provide cellular synaptic map in ventral nerve cord these examine how activating or inhibiting each cell type within affects song. Our data reveal circuit is organized into nested feedforward pathways with extensive reciprocal feedback connections. The larger network produces pulse song, more complex ancestral form. A subset this simpler recent Such organization may be feature which evolution has layered increasing flexibility onto basic pattern.

Language: Английский

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