Experimental Eye Research, Год журнала: 2022, Номер 224, С. 109247 - 109247
Опубликована: Сен. 13, 2022
Язык: Английский
Progress in Retinal and Eye Research, Год журнала: 2022, Номер 91, С. 101090 - 101090
Опубликована: Май 29, 2022
Язык: Английский
Процитировано
120
Experimental Eye Research, Год журнала: 2021, Номер 204, С. 108455 - 108455
Опубликована: Янв. 21, 2021
Язык: Английский
Процитировано
78
Current Gene Therapy, Год журнала: 2021, Номер 22(2), С. 104 - 131
Опубликована: Апрель 27, 2021
: This review provides comprehensive information about the advances in gene therapy anterior segment of eye, including cornea, conjunctiva, lacrimal gland, and trabecular meshwork. We discuss delivery systems, viral non-viral vectors as well editing techniques, mainly CRISPR-Cas9, epigenetic treatments, antisense siRNA therapeutics. also provide a detailed analysis various diseases where has been tested with corresponding outcomes. Disease conditions include corneal conjunctival fibrosis scarring, epithelial wound healing, graft survival, neovascularization, genetic dystrophies, herpetic keratitis, glaucoma, dry eye disease, other ocular surface diseases. Although most analyzed results on use validity at have obtained vitro or using animal models, we available human studies. Gene approaches are currently considered very promising emerging future treatments diseases, this field is rapidly expanding.
Язык: Английский
Процитировано
68
Advanced Drug Delivery Reviews, Год журнала: 2024, Номер 209, С. 115317 - 115317
Опубликована: Апрель 19, 2024
Fibrotic diseases are characterised by myofibroblast differentiation, uncontrolled pathological extracellular matrix accumulation, tissue contraction, scar formation and, ultimately / organ dysfunction. The cornea, the transparent located on anterior chamber of eye, is extremely susceptible to fibrotic diseases, which cause loss corneal transparency and often associated with blindness. Although topical corticosteroids antimetabolites extensively used in management fibrosis, they glaucoma, cataract formation, corneoscleral melting infection, imposing need far more effective therapies. Herein, we summarise discuss shortfalls recent advances vitro models (e.g. transforming growth factor-β (TGF-β) ascorbic acid interleukin (IL) induced) drug TGF-β inhibitors, epigenetic modulators) gene editing, silencing) therapeutic strategies fibrosis context. Emerging therapeutical agents neutralising antibodies, ligand traps, receptor kinase antisense oligonucleotides) that have shown promise clinical setting but not yet assessed context also discussed.
Язык: Английский
Процитировано
8
Biomedicines, Год журнала: 2022, Номер 10(2), С. 339 - 339
Опубликована: Фев. 1, 2022
Autophagy is a robust cellular mechanism for disposing of harmful molecules or recycling them to cells, which also regulates physiopathological processes in cornea. Dysregulated autophagy causes inefficient clearance unwanted proteins and debris, mitochondrial disorganization, defective inflammation, organ dysfunctions, cell death, diseases. The cornea accounts two-thirds the refraction light that occurs eyes, but prone trauma/injury infection. extracellular matrix (ECM) noncellular dynamic macromolecular network corneal tissues comprised collagens, proteoglycans, elastin, fibronectin, laminins, hyaluronan, glycoproteins. ECM undergoes remodeling by matrix-degrading enzymes maintains transparency. plays an important role wound healing maintenance. Delayed/dysregulated impacts healing, can lead dysfunction. Stromal involves responses from epithelium, basement membrane, keratocytes, ECM, many cytokines chemokines, including transforming growth factor beta-1 platelet-derived factor. Mild injuries self-repair, greater haze/scars/fibrosis vision loss due disruptions autophagy, normal processes. Presently, precise elusive. This review discusses recent trends modulation context homeostasis.
Язык: Английский
Процитировано
31
Cells, Год журнала: 2023, Номер 12(9), С. 1280 - 1280
Опубликована: Апрель 28, 2023
One of the most remarkable advancements in medical treatments corneal diseases recent decades has been transplantation. However, transplants, including lamellar strategies, have their own set challenges, such as graft rejection, delayed failure, shortage donor corneas, repeated treatments, and post-surgical complications. Corneal defects are one leading causes blindness globally; therefore, there is a need for gene-based interventions that may mitigate some these challenges help reduce burden blindness. Corneas being immune-advantaged, uniquely avascular, transparent ideal gene therapy approaches. Well-established surgical techniques well ease accessibility examination manipulation makes corneas suitable vivo ex therapy. In this review, we focus on advances area regeneration using strategies involved development therapies. We also discuss potential treatment diseases. Additionally, translational aspects therapy, different types vectors, particularly focusing recombinant AAV advance targeted therapeutics
Язык: Английский
Процитировано
19
Advanced Science, Год журнала: 2024, Номер 11(25)
Опубликована: Апрель 6, 2024
Abstract Corneal neovascularization (CNV) is a common clinical finding seen in range of eye diseases. Current therapeutic approaches to treat corneal angiogenesis, which vascular endothelial growth factor (VEGF) A plays central role, can cause variety adverse side effects. The technology Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 edit VEGFA gene suppress its expression. CRISPR offers novel opportunity CNV. This study shows that depletion with CRISPR/Cas9 system inhibits proliferation, migration, and tube formation human umbilical vein cells (HUVECs) vitro. Importantly, subconjunctival injection this dual AAV‐SpCas9/sgRNA‐ demonstrated blocks suture‐induced expression VEGFA, CD31, α‐smooth muscle actin as well mice. has established strong foundation for the treatment via editing approach first time.
Язык: Английский
Процитировано
7
Toxicology Letters, Год журнала: 2021, Номер 343, С. 21 - 27
Опубликована: Фев. 16, 2021
Язык: Английский
Процитировано
35
The Ocular Surface, Год журнала: 2024, Номер 32, С. 13 - 25
Опубликована: Янв. 6, 2024
Язык: Английский
Процитировано
5
Cell Death Discovery, Год журнала: 2024, Номер 10(1)
Опубликована: Апрель 8, 2024
Abstract Corneal diseases are among the primary causes of blindness and vision loss worldwide. However, pathogenesis corneal remains elusive, diagnostic therapeutic tools limited. Thus, identifying new targets for diagnosis treatment has gained great interest. Methylation, a type epigenetic modification, modulates various cellular processes at both nucleic acid protein levels. Growing evidence shows that methylation is key regulator in diseases, including inflammation, fibrosis, neovascularization, making it an attractive potential target. In this review, we discuss major alterations demethylation DNA, RNA, levels how these dynamics contribute to diseases. Also, provide insights into biomarkers may improve
Язык: Английский
Процитировано
4