Pentafluorophenyl-based single-chain polymer nanoparticles DOI Open Access

Jan-Willem D. Paats

Published: Oct. 11, 2023

Well defined single chain polymer nanoparticles ( SCNPs ) can be formed by intramolecular crosslinking of chains . These particles have a size around 10 nm, which is similar to proteins. equipped with widevariety functionalities, although the functionalization step, and mainly its quantification, not straightforward. This thesis describes method for creating easily modifiable surface groups, sy n thesize study sets offunctional biomedical applications. In Chapter 1 , we first describe current methods available modifying SCNPs.In 2 synthesis active ester groups (pentafluorophenyl esters, A series described increasing incorporation protonatable tertiary amine small amount amines exhibited low cellular uptake, their fate being lysosome. Particles high er showed significantly higher while simultaneously reaching cytosol.Chapter 3 investigates set amounts in blood brain barrier model. All very degree transcytosis compared other nanostructures tested on highest charge also cytosolic uptake however at expense slightly reducedChapter 4 combines strategy from controlled drug delivery an anticancer Only 40% (thus cytosol) decrease number HeLacancer cells. clearly demonstrates importance intracellular location effectiveness drugs.In 5 expanded combining chemistry click The were functionalized amino alkynes, then conjugated azido glucose via two different positions. Uptake studies cancer cells (HeLa) no distinction between conjugation positions, but that density determined uptake.Chapter 6 ways scale up SCNP production explore new potential Finally, vivo investigated mouse Despite smallsize, still present body after 24 hours. Increasing led increased liver spleen, indicating opsonization macrophages.

Language: Английский

Artificial cells for in vivo biomedical applications through red blood cell biomimicry DOI Creative Commons
Jorik Waeterschoot, Willemien Gosselé, Špela Lemež

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 20, 2024

Recent research in artificial cell production holds promise for the development of delivery agents with therapeutic effects akin to real cells. To succeed these applications, systems need survive circulatory conditions. In this review we present strategies that, inspired by endurance red blood cells, have enhanced viability large, cell-like vehicles vivo use, particularly focusing on giant unilamellar vesicles. Insights from cells can guide modifications that could transform platforms into advanced drug vehicles, showcasing biomimicry's potential shaping future applications.

Language: Английский

Citations

20
Beyond the EPR effect: Intravital microscopy analysis of nanoparticle drug delivery to tumors DOI Creative Commons
Iaroslav B. Belyaev, Olga Yu. Griaznova, Alexey V. Yaremenko

et al.

Advanced Drug Delivery Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 115550 - 115550

Published: Feb. 1, 2025

Language: Английский

Citations

4
In situ cellular hitchhiking of nanoparticles for drug delivery DOI Creative Commons
Edidiong Udofa, Zongmin Zhao

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 204, P. 115143 - 115143

Published: Nov. 24, 2023

Language: Английский

Citations

29
Effect of biophysical properties of tumor extracellular matrix on intratumoral fate of nanoparticles: Implications on the design of nanomedicine DOI
Yachao Tian,

Tianfu Cheng,

Fuwei Sun

et al.

Advances in Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 326, P. 103124 - 103124

Published: March 7, 2024

Language: Английский

Citations

14
Cell-mediated nanoparticle delivery systems: towards precision nanomedicine DOI Creative Commons
Ruoyu Cheng, Shiqi Wang

Drug Delivery and Translational Research, Journal Year: 2024, Volume and Issue: 14(11), P. 3032 - 3054

Published: April 13, 2024

Cell-mediated nanoparticle delivery systems (CMNDDs) utilize cells as carriers to deliver the drug-loaded nanoparticles. Unlike traditional drug approaches, CMNDDs take advantages of cell characteristics, such homing capabilities stem cells, inflammatory chemotaxis neutrophils, prolonged blood circulation red and internalization macrophages. Subsequently, can easily prolong circulation, cross biological barriers, blood-brain barrier bone marrow-blood barrier, rapidly arrive at diseased areas. Such advantageous properties make promising candidates for precision targeting. In this review, we summarize recent advances in fabrication biomedical applications. Specifically, ligand-receptor interactions, non-covalent covalent are commonly applied constructing vitro. By hitchhiking macrophages, monocytes, platelets, nanoparticles be internalized or attached construct vivo. Then highlight application treating different diseases, cancer, central nervous system disorders, lung cardiovascular with a brief discussion about challenges future perspectives end.

Language: Английский

Citations

14
Stimuli‐Responsive Nanocarriers for Transcytosis‐Based Cancer Drug Delivery DOI Creative Commons

Zhehao Wang,

Yuji Sun,

Youqing Shen

et al.

Advanced NanoBiomed Research, Journal Year: 2024, Volume and Issue: 4(3)

Published: Jan. 8, 2024

Significant challenges persist in enhancing the delivery efficiency of tumor nanomedicines, predominantly due to difficulty successfully surpassing pathophysiological barriers. Enhancing penetration nanomedicines such conditions represents a pivotal goal advancing anticancer nanotherapeutics. Transcytosis emerges as promising solution this context, addressing limitations passive drug delivery. By harnessing diverse stimuli induce transcytosis, nanocarriers can achieve precise and deep penetration, resulting high therapeutic efficacy reduced systemic exposure compound. This review briefly examines various stimuli‐responsive nanosystems offers an overview outlook on development for transcytosis‐based cancer delivery, aiming provide informative insights design capable tissue enhanced efficacy.

Language: Английский

Citations

13
Modulating Intracellular Dynamics for Optimized Intracellular Release and Transcytosis Equilibrium DOI

Guiping Yuan,

Minghui Li, Yifan Zhang

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(25)

Published: April 4, 2024

Active transcytosis-mediated nanomedicine transport presents considerable potential in overcoming diverse delivery barriers, thereby facilitating tumor accumulation and penetration. Nevertheless, the persistent challenge lies achieving a nuanced equilibrium between intracellular interception for drug release transcytosis In this study, comprehensive exploration is conducted involving series of polyglutamine-paclitaxel conjugates featuring distinct hydrophilic/hydrophobic ratios (HHR) tertiary amine-oxide proportions (TP) (OPGA-PTX). The screening process, meticulously focused on delineating their subcellular distribution, capability, penetration, unveils particularly promising candidate denoted as OPPX, characterized by an HHR 10:1 TP 100%. distinguished its rapid cellular internalization through multiple endocytic pathways, selectively engages trafficking to Golgi apparatus facilitate within penetration throughout tissues simultaneously sorted lysosomes cathepsin B-activated release. This study not only identifies OPPX exemplary but also underscores feasibility modulating distribution optimize active capabilities mechanisms nanomedicines, providing alternative approach designing efficient anticancer nanomedicines.

Language: Английский

Citations

11
Mitochondria‐Targeted Multifunctional Nanoparticles Combine Cuproptosis and Programmed Cell Death‐1 Downregulation for Cancer Immunotherapy DOI Creative Commons
Youyou Li, Jing Liu, Ralph R. Weichselbaum

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: July 16, 2024

The combination of cuproptosis and immune checkpoint inhibition has shown promise in treating malignant tumors. However, it remains a challenge to deliver copper ions inhibitors efficiently simultaneously Herein, mitochondria-targeted nanoscale coordination polymer particle, Cu/TI, comprising Cu(II), triphenylphosphonium conjugate 5-carboxy-8-hydroxyquinoline (TI), for effective induction programmed cell death-1 (PD-L1) downregulation is reported. Upon systemic administration, Cu/TI accumulates tumor tissues induce immunogenic cancer death reduce PD-L1 expression. Consequently, promotes the intratumoral infiltration activation cytotoxic T lymphocytes greatly inhibit progression colorectal carcinoma triple-negative breast mouse models without causing obvious side effects.

Language: Английский

Citations

11
Nano/genetically engineered cells for immunotherapy DOI Creative Commons

Jingrui Shen,

Yang Zhou, Lichen Yin

et al.

BMEMat, Journal Year: 2024, Volume and Issue: unknown

Published: July 17, 2024

Abstract Immunotherapy has recently emerged as a promising therapeutic modality for the treatment of various diseases such cancer, inflammation, autoimmune diseases, and infectious diseases. Despite its potential, immunotherapy faces challenges related to delivery efficiency off‐target toxicity immunotherapeutic drugs. Nano drug systems offer improvements in biodistribution release kinetics but still suffer from shortcomings high immunogenicity, poor penetration across biological barriers, insufficient tissue permeability. Targeted drugs using living cells become an emerging strategy that can take advantage inherent characteristics deal with defects nano systems. Furthermore, themselves be genetically engineered into cellular enhanced immunotherapy. This review provides in‐depth exploration cell‐derived carriers, detailing their properties, functions, commonly used loading strategies. In addition, role modified synergistic effects are also introduced. By summarizing main advancements limitations field, this offers insights potential cell‐based address existing The introduction recent developments evaluation ongoing research will pave way optimization widespread adoption nano/genetically

Language: Английский

Citations

11
Responsive Zwitterionic Materials for Enhanced Drug Delivery DOI
Ke Zheng, Xumei Ouyang, Xie Hong

et al.

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Zwitterionic materials have traditionally been recognized as exceptional antifouling agents, imparting nanocarriers with extended circulation times in vivo. Despite much studies on ability, the responsive zwitterionic that change physicochemical properties stimulated by mild signals are less explored. As is known, there multiple biological barriers antitumor drug delivery, including blood barrier, non-specific organ distribution, elevated tumor interstitial pressure, cytomembrane and lysosomal barrier. Multiple restrict delivery efficiency of to tumors, leading a reduced therapeutic effect increased side effects. Therefore, it far from satisfactory overcome barrier alone for classical materials. To address this challenge, recently developed engineered barriers, thereby enabling more effective delivery. Furthermore, could respond themselves without need incorporating extra stimuli-responsive groups, which maintains simplicity molecular structure. In mini-review, recent progress responding pH, temperature, enzyme, or reactive oxygen species summarized. prospects challenges provided promote better development field.

Language: Английский

Citations

1