Artificial Intelligence‐Assisted High‐Throughput Screening of Printing Conditions of Hydrogel Architectures for Accelerated Diabetic Wound Healing DOI

Baiqi Chen,

Jianpei Dong, Marina Ruelas

et al.

Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 32(38)

Published: July 10, 2022

Abstract In 3D (bio)printing, it is critical to optimize the printing conditions obtain scaffolds with designed structures and good uniformities. Traditional approaches for optimizing parameters oftentimes rely on prior knowledge of operators tedious optimization experiments, which can be both time‐consuming labor‐intensive. Moreover, rapid increase in types biomaterial inks geometrical complexities fabricated, such a traditional strategy may prove less effective. To address challenge, an artificial intelligence‐assisted high‐throughput printing‐condition‐screening system (AI‐HTPCSS) proposed, composed programmable pneumatic extrusion (bio)printer AI‐assisted image‐analysis algorithm. Based AI‐HTPCSS, obtaining uniformly structured hydrogel architectures are screened manner. The results show that printed under optimized demonstrate satisfying mechanical properties, vitro biological performances, efficacy accelerating diabetic wound healing vivo. unique AI‐HTPCSS expected offer enabling platform technology streamlining manufacturing tissue‐engineering through (bio)printing techniques future.

Language: Английский

Multimaterial 3D and 4D Bioprinting of Heterogenous Constructs for Tissue Engineering DOI
Annan Chen, Wanying Wang, Zhengyi Mao

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(34)

Published: Sept. 22, 2023

Additive manufacturing (AM), which is based on the principle of layer-by-layer shaping and stacking discrete materials, has shown significant benefits in fabrication complicated implants for tissue engineering (TE). However, many native tissues exhibit anisotropic heterogenous constructs with diverse components functions. Consequently, replication biomimetic using conventional AM processes a single material challenging. Multimaterial 3D 4D bioprinting (with time as fourth dimension) emerged promising solution constructing multifunctional that can mimic host microenvironment better than single-material alternatives. Notably, 4D-printed multimaterial architectures provide time-dependent programmable dynamic promote cell activity regeneration response to external stimuli. This paper first presents typical design strategies TE applications. Subsequently, latest are discussed, along their advantages challenges. In particular, potential smart highlighted. Furthermore, this review provides insights into how facilitate realization next-generation

Language: Английский

Citations

57
Emerging Bioprinting for Wound Healing DOI
Zijian Wang, Xiao Liang, Guanyi Wang

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 11, 2023

Bioprinting has attracted much attention due to its suitability for fabricating biomedical devices. In particular, bioprinting become one of the growing centers in field wound healing, with various types bioprinted devices being developed, including 3D scaffolds, microneedle patches, and flexible electronics. Bioprinted can be designed specific biostructures biofunctions that closely match shape sites accelerate regeneration skin through approaches. Herein, a comprehensive review smart dressings is presented, emphasizing crucial effect determining biofunctions. The begins an overview techniques devices, followed in-depth discussion polymer-based inks, modification strategies, additive ingredients, properties, applications. strategies are divided into seven categories, chemical synthesis novel physical blending, coaxial bioprinting, multimaterial absorption, immobilization, hybridization living cells, examples presented. Thereafter, frontiers 4D artificial intelligence-assisted situ discussed from perspective interdisciplinary sciences. Finally, current challenges future prospects this highlighted.

Language: Английский

Citations

54
Review on Porous Scaffolds Generation Process: A Tissue Engineering Approach DOI
Mariana S. Flores-Jiménez, Alejandro García, Rita Q. Fuentes‐Aguilar

et al.

ACS Applied Bio Materials, Journal Year: 2023, Volume and Issue: 6(1), P. 1 - 23

Published: Jan. 4, 2023

Porous scaffolds have been widely explored for tissue regeneration and engineering in vitro three-dimensional models. In this review, a comprehensive literature analysis is conducted to identify the steps involved their generation. The advantages disadvantages of available techniques are discussed, highlighting importance considering pore geometrical parameters such as curvature size, summarizing requirements generate porous scaffold according desired application. This paper considers design tools, mathematical models, materials, fabrication techniques, cell seeding methodologies, assessment methods, status clinical applications. review compiles relevant research field past years. trends, challenges, future directions discussed search generation with improved mechanical biological properties that can be reproducible, viable long-term studies, closer being used field.

Language: Английский

Citations

50
Advanced Soft Robotic System for In Situ 3D Bioprinting and Endoscopic Surgery DOI Creative Commons
Mai Thanh Thai, Phuoc Thien Phan, Hien A. Tran

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(12)

Published: Feb. 19, 2023

Three-dimensional (3D) bioprinting technology offers great potential in the treatment of tissue and organ damage. Conventional approaches generally rely on a large form factor desktop bioprinter to create vitro 3D living constructs before introducing them into patient's body, which poses several drawbacks such as surface mismatches, structure damage, high contamination along with injury due transport open-field surgery. In situ inside body is potentially transformational solution serves an excellent bioreactor. This work introduces multifunctional flexible (F3DB), features degree freedom soft printing head integrated robotic arm deliver multilayered biomaterials internal organs/tissues. The device has master-slave architecture operated by kinematic inversion model learning-based controllers. capabilities different patterns, surfaces, colon phantom are also tested composite hydrogels biomaterials. F3DB capability perform endoscopic surgery further demonstrated fresh porcine tissue. new system expected bridge gap field support future development advanced surgical robots.

Language: Английский

Citations

44
Nanogels: Synthesis, properties, and recent biomedical applications DOI
Qiu‐Yi Duan, Ya‐Xuan Zhu, Hao‐Ran Jia

et al.

Progress in Materials Science, Journal Year: 2023, Volume and Issue: 139, P. 101167 - 101167

Published: July 26, 2023

Language: Английский

Citations

44
Multi‐Material Volumetric Additive Manufacturing of Hydrogels using Gelatin as a Sacrificial Network and 3D Suspension Bath DOI Creative Commons

Morgan B. Riffe,

Matthew D. Davidson,

Gabriel Seymour

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(34)

Published: Jan. 20, 2024

Abstract Volumetric additive manufacturing (VAM) is an emerging layerless method for the rapid processing of reactive resins into 3D structures, where printing much faster (seconds) than other lithography and direct ink writing methods (minutes to hours). As a vial resin rotates in VAM process, patterned light exposure defines object then that has not undergone gelation can be washed away. Despite promise VAM, there are challenges with soft hydrogel materials from non‐viscous precursors, including multi‐material constructs. To address this, sacrificial gelatin used modulate viscosity support cytocompatible macromers based on poly(ethylene glycol) (PEG), hyaluronic acid (HA), polyacrylamide (PA). After printing, removed by washing at elevated temperature. print constructs, gelatin‐containing as shear‐yielding suspension bath (including HA further properties) extruded define processed defined object. Multi‐material constructs methacrylated (MeHA) methacrylamide (GelMA) printed (as proof‐of‐concept) encapsulated mesenchymal stromal cells (MSCs), local properties guide cell spreading behavior culture.

Language: Английский

Citations

33
Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications DOI
Sushila Maharjan,

Chenshuo Ma,

Bibhor Singh

et al.

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 208, P. 115237 - 115237

Published: March 5, 2024

Language: Английский

Citations

27
Nerve Growth Factor-Preconditioned Mesenchymal Stem Cell-Derived Exosome-Functionalized 3D-Printed Hierarchical Porous Scaffolds with Neuro-Promotive Properties for Enhancing Innervated Bone Regeneration DOI

Meifei Lian,

Zhiguang Qiao, Shichong Qiao

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(10), P. 7504 - 7520

Published: Feb. 27, 2024

The essential role of the neural network in enhancing bone regeneration has often been overlooked biomaterial design, leading to delayed or compromised healing. Engineered mesenchymal stem cells (MSCs)-derived exosomes are becoming increasingly recognized as potent cell-free agents for manipulating cellular behavior and improving therapeutic effectiveness. Herein, MSCs stimulated with nerve growth factor (NGF) regulate exosomal cargoes improve neuro-promotive potential facilitate innervated regeneration. In vitro cell experiments showed that NGF-stimulated MSCs-derived (N-Exos) obviously improved function neurotrophic effects cells, consequently, osteogenic osteo-reparative cells. Bioinformatic analysis by miRNA sequencing pathway enrichment revealed beneficial N-Exos may partly be ascribed NGF-elicited multicomponent miRNAs subsequent regulation activation MAPK PI3K-Akt signaling pathways. On this basis, were delivered on micropores 3D-printed hierarchical porous scaffold accomplish sustained release profile extended bioavailability. a rat model distal femoral defect, N-Exos-functionalized significantly induced neurovascular structure formation This study provided feasible strategy modulate functional acquire desirable potential. Furthermore, developed represent promising neurovascular-promotive reparative clinical translation.

Language: Английский

Citations

18
Shedding light on 3D printing: Printing photo-crosslinkable constructs for tissue engineering DOI
Qiang Zhang,

Ho‐Pan Bei,

Mengna Zhao

et al.

Biomaterials, Journal Year: 2022, Volume and Issue: 286, P. 121566 - 121566

Published: May 10, 2022

Language: Английский

Citations

69
Functional Trachea Reconstruction Using 3D‐Bioprinted Native‐Like Tissue Architecture Based on Designable Tissue‐Specific Bioinks DOI Creative Commons
Yingying Huo, Yong Xu, Xiaodi Wu

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(29)

Published: July 26, 2022

Abstract Functional segmental trachea reconstruction remains a remarkable challenge in the clinic. To date, functional regeneration with alternant cartilage‐fibrous tissue‐mimetic structure similar to that of native relying on three‐dimensional (3D) bioprinting technology has seen very limited breakthrough. This fact is mostly due lack tissue‐specific bioinks suitable for both cartilage and vascularized fibrous tissue regeneration, as well need firm interfacial integration between stiff soft tissues. Here, novel strategy developed 3D cartilage‐vascularized tissue‐integrated ( CVFIT ), utilizing photocrosslinkable bioinks. Both cartilage‐ created by this study provide printability, favorable biocompatibility, biomimetic microenvironments chondrogenesis fibrogenesis based multicomponent synergistic effect through hybrid photoinitiated polymerization reaction. As such, tubular analogs are successfully bioprinted ring‐to‐ring tightly integrated enhancement bonding amidation The results from situ demonstrate satisfactory along realization mechanical physiological functions. thus illustrates 3D‐bioprinted tissue‐like promising alternative clinical reconstruction.

Language: Английский

Citations

64