Trends in biotechnology,
Journal Year:
2024,
Volume and Issue:
42(10), P. 1241 - 1257
Published: April 23, 2024
Advances
in
tissue
engineering
for
both
system
modeling
and
organ
regeneration
depend
on
embracing
recapitulating
the
target
tissue's
functional
structural
complexity.
Microenvironmental
features
such
as
anisotropy,
heterogeneity,
other
biochemical
mechanical
spatiotemporal
cues
are
essential
regulating
development
function.
Novel
biofabrication
strategies
innovative
biomaterial
design
have
emerged
promising
tools
to
better
reproduce
features.
These
facilitate
a
transition
towards
high-fidelity
biomimetic
structures,
offering
opportunities
deeper
understanding
of
function
superior
therapies.
In
this
review,
we
explore
some
key
compositional
aspects
tissues,
lay
out
how
achieve
similar
outcomes
with
current
fabrication
strategies,
identify
main
challenges
avenues
future
research.
Chemical Reviews,
Journal Year:
2024,
Volume and Issue:
124(14), P. 8787 - 8822
Published: July 5, 2024
Harnessing
light
for
cross-linking
of
photoresponsive
materials
has
revolutionized
the
field
3D
printing.
A
wide
variety
techniques
leveraging
broad-spectrum
shaping
have
been
introduced
as
a
way
to
achieve
fast
and
high-resolution
printing,
with
applications
ranging
from
simple
prototypes
biomimetic
engineered
tissues
regenerative
medicine.
Conventional
light-based
printing
use
material
in
layer-by-layer
fashion
produce
complex
parts.
Only
recently,
new
emerged
which
deploy
multidirection,
tomographic,
light-sheet
or
filamented
image
projections
deep
into
volume
resin-filled
vat
photoinitiation
cross-linking.
These
Deep
Vat
(DVP)
approaches
alleviate
need
layer-wise
enable
unprecedented
fabrication
speeds
(within
few
seconds)
high
resolution
(>10
μm).
Here,
we
elucidate
physics
chemistry
these
processes,
their
commonalities
differences,
well
emerging
biomedical
non-biomedical
fields.
Importantly,
highlight
limitations,
future
scope
research
that
will
improve
scalability
applicability
DVP
engineering
medicine
applications.
Advanced Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 26, 2025
Abstract
Volumetric
Bioprinting
(VBP),
enables
to
rapidly
build
complex,
cell‐laden
hydrogel
constructs
for
tissue
engineering
and
regenerative
medicine.
Light‐based
tomographic
manufacturing
spatial‐selective
polymerization
of
a
bioresin,
resulting
in
higher
throughput
resolution
than
what
is
achieved
using
traditional
techniques.
However,
methods
multi‐material
printing
are
needed
broad
VBP
adoption
applicability.
Although
converging
with
extrusion
bioprinting
support
baths
offers
novel,
promising
solution,
further
knowledge
on
the
hydrogels
as
light‐responsive,
volumetrically
printable
needed.
Therefore,
this
study
investigates
tuning
gelatin
macromers,
particular
leveraging
effect
molecular
weight
degree
modification,
overcome
these
challenges,
creating
library
materials
Embedded
Printing
(EmVP).
Bioresins
tunable
printability
mechanical
properties
produced,
novel
subset
gelatins
GelMA
exhibiting
stable
shear‐yielding
behavior
new,
single‐component,
ready‐to‐use
suspension
medium
in‐bath
printing,
which
over
multiple
hours
without
needing
temperature
control.
As
proof‐of‐concept
biological
application,
bioprinted
gels
tested
insulin‐producing
pancreatic
cell
lines
21
days
culture.
Leveraging
multi‐color
printer,
complex
multi‐cellular
geometries
enhancing
accessibility
volumetric
advanced
models.
Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
13(20)
Published: April 22, 2024
Regenerative
medicine
has
evolved
with
the
rise
of
tissue
engineering
due
to
advancements
in
healthcare
and
technology.
In
recent
years,
bioprinting
been
an
upcoming
approach
traditional
practices,
through
fabrication
functional
by
its
layer-by-layer
deposition
process.
This
overcomes
challenges
such
as
irregular
cell
distribution
limited
density,
it
can
potentially
address
organ
shortages,
increasing
transplant
options.
Bioprinting
fully
organs
is
a
long
stretch
but
advancement
rapidly
growing
precision
compatibility
complex
geometries.
Computational
Fluid
Dynamics
(CFD),
carestone
computer-aided
engineering,
instrumental
assisting
research
development
cutting
costs
saving
time.
CFD
optimizes
testing
parameters
shear
stress,
diffusivity,
viability,
reducing
repetitive
experiments
aiding
material
selection
bioprinter
nozzle
design.
review
discusses
current
application
potential
enhance
technology
that
contribute
evolution
regenerative
medicine.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 2, 2024
Abstract
The
effective
replication
of
microtubular
structures
in
tissue
engineering
remains
a
great
challenge.
In
this
study,
the
temperature‐responsive
characteristics
poly(
N
‐isopropylacrylamide)
(pNIPAM)
to
create
intricate,
high‐resolution
tubular
through
shrinking
mechanism
is
investigated
by
exploring
2
thermosensitive
hydrogels–gelatin
methacryloyl
(gelMA)
and
silk
fibroin
(silkMA)–combined
with
pNIPAM.
Systematic
investigations
revealed
precise
control
behavior
at
elevated
temperatures
(33–37
°C)
as
function
polymer
concentration.
hydrogel
sizes
reduce
≈15%
from
room
temperature
(RT)
33
°C
≈40%
RT
37
for
both
types.
affects
mechanical
properties,
increasing
compressive
modulus
≈2.8‐fold
gelMA‐pNIPAM
gels
≈5.1‐fold
silkMA‐pNIPAM
°C.
Combined
volumetric
printing,
these
materials
achieve
resolution
enhancements
≈20%
positive
features
≈70%
negative
features,
enabling
creation
complex,
within
seconds,
open
channels
(≈50
µm).
GelMA‐pNIPAM
hydrogels
show
better
cell
compatibility
compared
hydrogels,
promoting
adhesion
viability.
This
study
demonstrates
hydrogels'
capability
engineer
printing–an
efficient
route
fabricate
microenvironments
mimicking
native
tissues
potential
developing
relevant
vitro
models.
Nano Select,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 4, 2025
ABSTRACT
To
expand
the
use
of
collagen‐based
biomaterials
beyond
their
current
applications
in
three‐dimensional
(3D)
cell
culture,
tissue
engineering,
and
biofabrication,
limitations
such
as
poor
shear‐thinning
behavior
control
over
porosity
during
gelation
need
to
be
overcome.
Granular
promise
address
these
constraints,
however
uniform
scalable
preparation
from
extracellular
matrix
materials
is
challenging.
this
need,
we
employed
a
droplet
microfluidic
approach
prepared
irregularly
shaped
microgels
fibrillar
collagen
collagen‐glycosaminoglycan
(GAG)
copolymer
continuous
oil
phase,
at
rates
up
5500
s
−1
.
The
allowed
us
tune
average
microgel
size
40
170
µm.
Microgels
obtained
after
removal
phase
were
found
promote
attachment
proliferation
human
fibroblasts
mesenchymal
stromal/stem
cells.
with
packing
densities
exceeding
65
vol%
exhibited
rheological
behavior,
requirement
for
injectable
bioinks.
Cell‐containing
granular
contracted
2.8
times
less
than
thermally
gelled
matrices
comparable
concentration.
In
case
study,
skin
model
fibroblast
containing
collagen‐GAG
(CG)
layer
covered
an
epithelium
revealed
immunohistochemical
markers
associated
intact
month‐long
air–liquid
interface
(ALI)
culture.
