“Zero” Intrinsic Fluorescence Sensing‐Platforms Enable Ultrasensitive Whole Blood Diagnosis and In Vivo Imaging DOI
Gangwei Jiang, Hong Liu,

Guohui Deng

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(16)

Published: Feb. 27, 2024

Abnormal physiological processes and diseases can lead to content or activity fluctuations of biocomponents in organelles whole blood. However, precise monitoring these abnormalities remains extremely challenging due the insufficient sensitivity accuracy available fluorescence probes, which be attributed background arising from two sources, 1) biocomponent autofluorescence (BCAF) 2) probe intrinsic (PIF). To overcome obstacles, we have re-engineered far-red NIR II rhodol derivatives that possess weak BCAF interference. And a series "zero" PIF sensing-platforms were created by systematically regulating open-loop/spirocyclic forms. Leveraging advancements, devised various ultra-sensitive indicators, achieving substantial boosts (190 1300-fold). Among 8-LAP demonstrated accurate tracking quantifying leucine aminopeptidase (LAP) blood at stages tumor metastasis. Furthermore, coupling with an endoplasmic reticulum-targeting element enabled detection ERAP1 HCT116 cells p53 abnormalities. This delicate design eliminating provides insights into enhancing existing probes toward imaging abnormal diseases.

Language: Английский

Development of Polymethine Dyes for NIR‐II Fluorescence Imaging and Therapy DOI
Xin Chen,

Jieyan Li,

Shubham Roy

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(16)

Published: March 5, 2024

Fluorescence imaging in the second near-infrared window (NIR-II) is burgeoning because of its higher fidelity monitoring physiological and pathological processes than clinical visible/the fluorescence imaging. Notably, heavily dependent on agents. So far, indocyanine green, one polymethine dyes, with good biocompatibility renal clearance only dye approved by Food Drug Administration, but it shows relatively low NIR-II brightness. Importantly, tremendous efforts are devoted to synthesizing dyes for preclinically clinically. They have shown feasibility customization structure properties fulfill various needs therapy. Herein, a timely update special focus molecular design strategies fluorescent, photoacoustic, multimodal imaging, offered. Furthermore, progress sensing biomarkers even reporting drug release illustrated. Moreover, imaging-guided therapies summarized regarding chemo-, photothermal, photodynamic, approaches. In addition, artificial intelligence pointed out potential expedite development. This comprehensive review will inspire interest among wide audience offer handbook people an dyes.

Language: Английский

Citations

23
2, 1, 3‐Benzothiadiazole Derivative Small Molecule Fluorophores for NIR‐II Bioimaging DOI

Zhen Hu,

Lili Feng, Piaoping Yang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(16)

Published: Jan. 4, 2024

Abstract Second near‐infrared (NIR‐II) bioimaging technology shows broad application prospects in the field of biomedical due to its significant superiority deep tissue penetration and high signal‐to‐background ratio. Among them, organic small molecule fluorophores have received progressively more consideration their low biotoxicity, biocompatibility, rapid clearance, good modification properties. As key acceptor core, 2, 1, 3‐benzothiadiazole (BTD) derivative plays an essential role construction donor‐acceptor‐donor type NIR‐II fluorophores. This mini‐review summarizes research development based on BTD derivatives bioimaging, focusing molecular design photophysical properties, introduces a part applications biological field. Furthermore, challenges BTD‐based future are discussed .

Language: Английский

Citations

21
Biomedical applications of NIR-II organic small molecule fluorescent probes in different organs DOI
Lizhen Xu, Qian Zhang, Xin Wang

et al.

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 519, P. 216122 - 216122

Published: Aug. 6, 2024

Language: Английский

Citations

20
Advances in Organic Small Molecule-Based Fluorescent Probes for Precision Detection of Liver Diseases: A Perspective on Emerging Trends and Challenges DOI Creative Commons
Luling Wu, Zilu Li, Kun Wang

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Liver disease poses a significant challenge to global health, and its early diagnosis is crucial for improving treatment outcomes patient prognosis. Since fluctuation of key biomarkers during the onset progression liver diseases can directly reflect health normal/abnormal function, biomarker-based assays are vital tools detection disease. In this context, small molecule fluorescent probes have undeniably emerged as indispensable analysis, with an ever-growing number molecule-based being developed over recent years, sole aim monitoring relevant This perspective will focus on development application primarily last 10 years diagnosing range disease-related processes. It outline foundational design strategies developing promising probes, their optical response biomarkers, how they been demonstrated in proof-of-concept imaging applications. Current challenges new developments field be discussed, providing insights highlighting opportunities field.

Language: Английский

Citations

3
Super-Resolution Imaging of Mitochondrial HClO during Cell Ferroptosis Using a Near-Infrared Fluorescent Probe DOI

Hongbao Fang,

Yuncong Chen, Shanshan Geng

et al.

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(51), P. 17904 - 17912

Published: Dec. 8, 2022

Ferroptosis is of great importance in physiological and pathological processes, which associated with various inflammation-related diseases, cardiovascular even cancer. can cause abnormal change reactive oxygen species (ROS) mitochondria. Hypochlorous acid (HClO) acts as a typical ROS. Therefore, it needed to study the relationship between mitochondrial morphology HClO changes during ferroptosis at subcellular level. To this end, near-infrared-excitation/emission fluorescent probe, HD-Br-1, for rapid detection was developed based on specific oxidative cleavage N,N-dimethylthiocarbamate moiety. The fluctuation content were monitored real time by super-resolution imaging. In addition, HD-Br-1 successfully applied monitor exogenous endogenous cell visualize tumor discriminate from healthy tissues. we believe that could provide valuable approach cancer cells well understanding mechanism early diagnosis cancers future research.

Language: Английский

Citations

66
Near‐Infrared Carbonized Polymer Dots for NIR‐II Bioimaging DOI
Tianyang Han, Yajun Wang, Shengjie Ma

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(30)

Published: Sept. 1, 2022

Abstract Carbon dots (CDs) or carbonized polymer (CPDs) are an emerging class of optical materials that have exceptional applications in optoelectronic devices, catalysis, detection, and bioimaging. Although cell studies CPDs produced impressive results, vivo imaging requires available to fluoresce the near‐infrared‐II (NIR‐II) window (1000−1700 nm). Here, a two‐step bottom‐up strategy is developed synthesize NIR‐CPDs provide bright emissions both NIR‐I NIR‐II transparent windows. The designed includes hydrothermal reaction form stable carbon core with aldehyde groups, followed by Knoevenagel tether molecular emission centers. This procedure labor‐saving, cost‐efficient, produces high yield. enable high‐performance angiography real‐time disease degree colitis noninvasively. technology may therefore next‐generation synthesis for rational engineering can accurately tune absorption/emission properties NIR‐emissive CPDs.

Language: Английский

Citations

62
Molecular Design of NIR-II Polymethine Fluorophores for Bioimaging and Biosensing DOI Creative Commons

Yifeng Ou,

Tian‐Bing Ren, Lin Yuan

et al.

Chemical & Biomedical Imaging, Journal Year: 2023, Volume and Issue: 1(3), P. 220 - 233

Published: May 18, 2023

Organic polymethine fluorophores with emission in the NIR-II window (1000–1700 nm) are receiving enormous attention biomolecular medicine and bioimaging, attributed to high absorption coefficients, bright emission, excellent biocompatibility, molecule adjustability. Recently, researchers have devoted significant effort designing improving making notable progress fluorescence imaging performance. This review summarizes recent developments molecular engineering design mentality of probes highlights their extensive bioimaging biosensing applications. Furthermore, we elucidate potential challenges perspectives these dyes chemical biomedicine imaging, which may stimulate further development high-performance dye contrast agents for future clinical

Language: Английский

Citations

40
THQ–Xanthene: An Emerging Strategy to Create Next‐Generation NIR‐I/II Fluorophores DOI Creative Commons
Zhiqiang Mao, Hyeonji Rha, Jungryun Kim

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(18)

Published: April 28, 2023

Near-infrared fluorescence imaging is vital for exploring the biological world. The short emissions (<650 nm) and small Stokes shifts (<30 of current xanthene dyes obstruct their applications since a long time. Recently, potent universal THQ structural modification technique that emission to NIR-I/II range enables substantial shift (>100 THQ-modified established. Thus, timely discussion THQ-xanthene its extensive. Hence, advent, working principles, development trajectory, dyes, especially in fields probe-based sensing imaging, cancer theranostics, super-resolution are introduced. It envisioned tactic simple yet exceptional approach upgrade performance conventional dyes. will advance strides xanthene-based potentials early fluorescent diagnosis diseases, imaging-guided surgery.

Language: Английский

Citations

39
Heptamethine Cyanine Dyes with Ultra‐Efficient Excited‐State Nonradiative Decay for Synergistic Photothermal Immunotherapy DOI
Xiang Xia, Chao Shi, Yang Zi

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(38)

Published: May 26, 2023

Abstract Organic dyes in the excited singlet state (S 1 ) decay via intersystem crossing (ISC) to triplet state, radiative dissipation as fluorescence, or nonradiative for thermal deactivation. Although many studies are conducted improve ISC and fluorescence efficiency, few have optimized S deactivation, which is crucial designing photothermal agents. A strategy inhibiting by introducing electron withdraw groups (EWGs) into meso position of heptamethine cyanines (Cy7) reported here, allows energy relaxation. The decrease density Cy7 caused EWGs improved photostability, important biological applications because conventional cyanine easily photobleached. EWG substitutes acted efficient rotation with low‐energy barriers, narrowing gap between ground considerably improving conversion efficiency (PCE). PCE CF 3 cy strongest increased up 83%. Liposome used a carrier biocompatibility tumor retention cy, combined toll‐like receptor agonist resiquimod (R848) synergistic immunotherapy against both primary distant tumors elicits long‐lasting immunological memory effect.

Language: Английский

Citations

36
“Dual‐Key‐and‐Lock” NIR‐II NSCyanines Enable High‐Contrast Activatable Phototheranostics in Extrahepatic Diseases DOI Open Access
Yang Tian, Zhaoming Chen,

Senyao Liu

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(38)

Published: Aug. 10, 2023

Conventional cyanine dyes with a symmetric structure are "always-on", which can easily accumulate in the liver and display high background fluorescence, inevitably interfering accurate diagnosis therapy extrahepatic diseases. We herein report platform of NIR-II non-symmetric (NSCyanine) by harnessing strategy, extremely sensitive to pH/viscosity be activated via "dual-key-and-lock" strategy. These NSCyanine low pKa (<4.0) only show weak fluorescence at lysosome pH (key1), however, completely switched on significantly enhanced intracellular viscosity (key2) disease tissues, exhibiting target-to-liver ratios up 19.5/1. Notably, high-contrast phototheranostics diseases achieved, including intestinal metastasis-imaging, acute gastritis-imaging, bacteria infected wound healing, tumor ablation targeted combined photothermal chemotherapy.

Language: Английский

Citations

29