Chemical metabolite synthesis and profiling: Mimicking in vivo biotransformation reactions
Bioorganic Chemistry,
Journal Year:
2023,
Volume and Issue:
139, P. 106722 - 106722
Published: July 8, 2023
Language: Английский
Etiopathology, Epidemiology, Diagnosis, and Treatment of Fungal Keratitis
ACS Infectious Diseases,
Journal Year:
2024,
Volume and Issue:
10(7), P. 2356 - 2380
Published: June 7, 2024
Fungal
keratitis
(FK)
is
a
severe
ocular
condition
resulting
from
corneal
infection
that
prevalent
in
tropical
countries,
particularly
developing
regions
of
Asia
and
Africa.
Factors
like
lens
misuse,
inappropriate
steroid
use,
diagnostic
challenges
have
provoked
the
epidemic.
FK
causes
significant
vision
impairment,
scarring,
deformities.
Accurate
pathological
diagnosis
crucial
for
effective
therapeutic
intervention.
Topical
antifungal
therapy
with
surface
healing
medications
proves
preventing
fungal-borne
ulcers.
Managing
requires
comprehensive
understanding
fungal
pathogenesis,
guiding
formulation
strategies
preventive
measures
to
curb
global
blindness.
This
review
provides
in-depth
insights
into
FK,
covering
etiology,
epidemiology,
interventions,
resistance,
limitations,
prevention,
future
perspectives
on
disease
management.
Language: Английский
Ocular Bioanalysis of Moxifloxacin and Ketorolac Tromethamine in Rabbit Lacrimal Matrix Using Liquid Chromatography–Tandem Mass Spectrometry
Bioanalysis,
Journal Year:
2024,
Volume and Issue:
16(4), P. 219 - 232
Published: Jan. 10, 2024
Aim:
The
fixed-dose
combination
of
moxifloxacin
(MOXI)
and
ketorolac
tromethamine
(KTR)
is
widely
used
for
the
treatment
bacterial
keratitis.
Thus,
a
new
LC–MS/MS
method
was
developed
to
determine
MOXI
KTR
in
lacrimal
fluid.
Methods:
Bioanalysis
performed
using
Shimadzu
8050
electrospray
ionization-positive
mode
validated
per
US
FDA
guidelines.
Isocratic
separation
with
Waters
Symmetry
C18
column
methanol
0.1%
formic
acid
containing
deionized
water
(85:15,
v/v).
Results
&
conclusion:
An
easy,
quick
selective
established
applied
assess
ocular
pharmacokinetic
profile
commercially
available
formulation
KTR.
Based
on
data,
this
work
describes
pharmacokinetics-based
dosage
regimen
calculations
their
clinical
significance.
Language: Английский
Codetermination of antimicrobial agents in rabbit tear fluid using LC–MS/MS assay: Insights into ocular pharmacokinetic study
Journal of Mass Spectrometry,
Journal Year:
2024,
Volume and Issue:
59(6)
Published: May 10, 2024
Abstract
Managing
ocular
microbial
infections
typically
requires
pharmacotherapy
using
antibiotic
eye
drops,
such
as
moxifloxacin
hydrochloride
(MFX),
combined
with
an
antifungal
agent
like
amphotericin
B
(AB).
We
carried
out
and
validated
LC–MS/MS
assay
to
quantify
these
compounds
in
rabbit
tear
fluid
order
look
into
the
pharmacokinetics
of
two
drugs.
employed
a
protein
precipitation
technique
for
extraction
drugs
under
examination.
A
Waters
Symmetry
C
18
column
was
used
separate
analytes
internal
standard.
The
composition
mobile
phase
(A)
0.1%
v/v
formic
acid
water
(B)
methanol.
detection
MFX
AB
accomplished
through
utilization
positive
ion
electrospray
ionization
multiple
reaction
monitoring
mode.
linearity
curves
both
exhibited
acceptable
trendline
across
concentration
range
2.34–300
ng/mL
7.81–1000
surrogate
fluid.
lower
limit
quantitation
2.34
ng/mL,
while
AB,
it
7.81
ng/mL.
approach
strictly
validated,
encompassing
tests
selectivity,
(with
r
2
>
0.99),
precision,
accuracy,
matrix
effects,
stability.
Consequently,
we
this
method
evaluate
profiles
following
single
topical
doses.
Language: Английский
Recent updates on ocular disease management with ophthalmic ointments
Therapeutic Delivery,
Journal Year:
2024,
Volume and Issue:
15(6), P. 463 - 480
Published: June 2, 2024
Ophthalmic
diseases
can
result
in
permanent
vision
loss
and
blindness.
Convenient
topical
systemic
treatments
are
preferred
to
address
these
sight-threatening
conditions.
However,
the
unique
anatomy
of
eye
presents
challenges
for
drug
delivery.
Various
ophthalmic
ointment
formulations
have
been
developed
enhance
bioavailability
prolong
residence
time
improve
corneal
permeability.
This
article
explores
a
wide
range
ocular
affecting
individuals
globally
how
ointments
used
manage
them.
From
barriers,
this
review
focuses
on
published
scientific
research
formulation
strategies
severe
complications
using
conventional
ointments.
Additionally,
it
delves
through
patented
technologies
marketed
supporting
use
Language: Английский
Impact of the COVID-19 Pandemic on Gut Cancer Admissions and Management: A Comparative Study of Two Pandemic Years to a Similar Pre-Pandemic Period
Healthcare,
Journal Year:
2025,
Volume and Issue:
13(7), P. 805 - 805
Published: April 3, 2025
Background/Objective:
Gastrointestinal
tract
cancers
may
have
been
severely
affected
by
the
COVID-19
pandemic.
The
limitations
of
digestive
endoscopy,
fear
effect,
and
restrictions
on
hospital
admissions
during
pandemic
delayed
presentation
patients
to
hospitals
surgical
procedures
impacted
overall
survival.
Methods:
We
conducted
an
observational,
cross-sectional
study
esophageal,
gastric,
small
bowel,
colorectal
cancer
admitted
our
between
1
January
2018
31
December
2021.
analyzed
hospitalization
rates,
pathological
type,
onset
complications,
staging,
surgery
compared
a
pre-pandemic
period
(January
2018-December
2019).
Results:
During
2018-2021,
1613
with
malignant
gut
tumors
were
(112
esophageal
eso-cardial
tumors,
419
gastric
34
bowel
1058
tumors).
Admission
was
reduced
30.3%
for
27.6%
17.3%
tumors.
For
higher
frequency
stenosing
palliative
gastrostomies
noted.
More
stage
III
lower
rate
vascular
invasion
recorded
No
differences
regarding
In
slightly
more
II
recorded,
but
occlusive,
bleeding,
perforated
similar;
also,
rates
similar,
two-fold
perioperative
mortality.
survival
carcinoma
(but
no
statistical
significance),
although
clear
explanation
has
not
emerged.
Conclusions:
impact
included
significantly
newly
diagnosed
admissions,
carcinomas,
mortality
carcinoma,
trend
surprisingly
without
significance).
Future
research
is
necessary
assessing
long-term
impact.
Language: Английский
Hesperidin: Enrichment, forced degradation, and structural elucidation of potential degradation products using spectral techniques
Rapid Communications in Mass Spectrometry,
Journal Year:
2023,
Volume and Issue:
37(20)
Published: Aug. 31, 2023
Hesperidin
(HES)
is
a
well-known
citrus
bioflavonoid
phyto-nutraceutical
agent
with
polypharmacological
properties.
After
2019,
HES
was
widely
used
for
prophylaxis
and
COVID-19
treatment.
Moreover,
it
commonly
prescribed
treating
varicose
veins
other
diseases
in
routine
clinical
practice.
Pharmaceutical
impurities
degradation
products
(DP)
impact
the
drug's
quality
safety
thus
its
effectiveness.
Therefore,
forced
studies
help
study
drug
stability,
mechanisms,
their
DPs.
This
performed
because
stress
stability
using
detailed
structural
characterization
of
hesperidin
are
currently
unavailable
literature.In
enrichment
method
crude
converted
to
pure
form
(98%
purity)
column
chromatography
then
subjected
under
acid,
base,
neutral
hydrolyses
followed
by
oxidative,
reductive,
photolytic,
thermal
testing
(International
Conference
on
Harmonization
guidelines).
The
stability-indicating
analytical
(SIAM)
developed
determine
DPs
reversed-phase
high-performance
liquid
(C18
methanol
0.1%
v/v
acetic
acid
deionized
water
[70:30,
v/v]
at
284
nm).
Further,
chromatography-electrospray
ionization-tandem
mass
spectrometry
(LC-ESI-MS/MS)
nuclear
magnetic
resonance
(NMR)
spectroscopy.
In
addition,
silico
toxicity
predictions
were
pKCSM
DataWarior
freeware.HES
found
be
susceptible
acidic
basic
hydrolytic
conditions
yielded
three
each,
which
detected
designed
SIAM.
Of
six
DPs,
pseudo-DPs
(short
lived),
remaining
characterized
LC-MS/MS
NMR
tentative
mechanism
formation
proposed
explained.
inactive
from
predictions.Hesperidin
labile
conditions.
potential
LC-ESI-MS/MS
spectral
techniques.
hypothesized.
identify
characterize
SIAM,
has
broad
biomedical
applications,
successfully
developed.
Language: Английский
Bio-Chemoinformatics-Driven Analysis of nsp7 and nsp8 Mutations and Their Effects on Viral Replication Protein Complex Stability
Current Issues in Molecular Biology,
Journal Year:
2024,
Volume and Issue:
46(3), P. 2598 - 2619
Published: March 18, 2024
The
nonstructural
proteins
7
and
8
(nsp7
nsp8)
of
SARS-CoV-2
are
highly
important
involved
in
the
RNA-dependent
polymerase
(RdRp)
protein
replication
complex.
In
this
study,
we
analyzed
global
mutation
nsp7
nsp8
2022
2023
effects
on
viral
complex
using
bio-chemoinformatics.
Frequently
occurring
variants
found
to
be
single
amino
acid
mutations
for
both
nsp8.
most
frequently
which
include
L56F,
L71F,
S25L,
M3I,
D77N,
V33I
T83I
predicted
cause
destabilizing
effects,
whereas
those
stabilizing
with
threonine
isoleucine
(T89I,
T145I,
T123I,
T148I,
T187I)
being
a
frequent
mutation.
A
conserved
domain
database
analysis
generated
critical
interaction
residues
(Lys-7,
His-36
Asn-37)
(Lys-58,
Pro-183
Arg-190),
which,
according
thermodynamic
calculations,
prone
destabilization.
Trp-29,
Phe-49
Trp-154,
Tyr-135
Phe-15
greater
based
computational
alanine
scan
suggesting
them
as
possible
new
target
sites.
This
study
provides
an
intensive
their
implications
stability.
Language: Английский