Antibiotic resistant bacteria in food systems: Current status, resistance mechanisms, and mitigation strategies

Agriculture Communications, Journal Year: 2024, Volume and Issue: 2(1), P. 100027 - 100027

Published: Feb. 3, 2024

The pervasive use of antibiotics in agriculture and animal husbandry has raised a significant concern—residual antibiotic contamination food, which contributes to the natural evolution resistance pathogenic microbial strains. emergence communities poses global threat food safety security. Recently, situation been exacerbated by discovery novel strains resistant bacteria (ARB) plant- animal-derived foods. These microbes can enter human body through direct contact with affected animals or consumption contaminated In this review, we explore prevalence contaminants at various locations around world, delve into molecular mechanisms behind acquisition antimicrobial resistance, examine current strategies employed mitigate spread pathogens, discuss emerging technologies aimed halting trend that projects 10 million annual deaths 2050 as result ARB agriculture. Genetic processes, including mutations, efflux pump activity, horizontal gene transfer, play crucial roles widespread distribution community. Effectively addressing requires development methodologies rapidly detect supply chain. Therefore, several established rapid diagnostic techniques such Quick, Easy, Cheap, Effective, Rugged, Safe (QuEChERS) methodology, aptasensors, fluorescence-based Metal Organic Frameworks. Additionally, innovative fight nano-antibiotics, antibiotics, synthetic biology, bacteriophages, predator bacteria. Here, propose omics biochar potential tools for combating ARB. We anticipate review article will serve valuable resource future research, particularly designed not only suppress activities genes but also potentially reverse are already communities.

Language: Английский

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Low‐Dose Cu Ions Assisted by Mild Thermal Stimulus Inducing Bacterial Cuproptosis‐Like Death for Antibiosis and Biointegration

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 34(1)

Published: Sept. 15, 2023

Abstract Methicillin‐resistant Staphylococcus aureus (MRSA) is threatening human health due to its resistance multiple antibiotics. Excessive copper (Cu) ions target the lipoylated proteins of tricarboxylic acid cycle cancer cells, inducing proteotoxic stress and their cuproptosis death. Whether plays a part in killing MRSA by low‐dose supplement Cu remains be explored. Herein, Cu‐doped hydroxyapatite nanorods (PC) are prepared on polyetheretherketone (PEEK) resist infection improve PEEK performance tissue integration with assistance near‐infrared (NIR) irradiation, mechanism against elucidated. Mild photothermal stimulation increases bacterial membrane permeability, accelerating ions’ intake consequently cuproptosis‐like death MRSA. It confirmed aggregation dihydrolipoamide S‐acetyltrans‐ferase (DLAT), deactivation glutathione peroxides 4 (GPX4), destabilization Fe─S cluster ferredoxin (FDX1) lipoyl synthase (LIAS). Fortunately, fibroblast behaviors upregulated NIR‐irradiated PC. In vivo, PC NIR irradiation exhibits outstanding elimination, reduced inflammation response, improved biointegration. Overall, it demonstrated that can induced at non‐cytotoxic dose when cooperated mild heat stimulus, this strategy has greatly promising application improving clinic.

Language: Английский

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Nitric Oxide‐Assisted Photodynamic Therapy for Enhanced Penetration and Hypoxic Bacterial Biofilm Elimination

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(29)

Published: July 29, 2023

The presence of a biofilm matrix barrier and hypoxic microenvironment within the significantly impedes efficacy photodynamic therapy for bacterial infections. Herein, phototherapeutic nanoagent with type-I behavior nitric oxide (NO) release performance is reported overcoming biofilm-associated infectious diseases. Sodium nitroprusside (SNP), NO donor, loaded onto amino-modified mesoporous silica nanoparticles (MSN) to form MSN@SNP NPs. resulting are further modified porphyrin-based metal-organic framework (Ti-TCPP MOF) obtain MSN@MOF/SNP NPs (MMS NPs) applications. In hypoxia microenvironment, MMS enhance permeability induce generation hydroxyl radical (•OH) superoxide anion (O2•- ) via Type-I pathway under laser irradiation. Subsequently, infections effectively eliminated through reactive oxygen species (ROS) gas synergistic therapy. addition, also stimulates collagen deposition promotes angiogenesis in vivo. Therefore, efficiently treat biofilm-related infections, providing an alternative approach combat

Language: Английский

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Enhanced lysosomal escape of cell penetrating peptide-functionalized metal–organic frameworks for co-delivery of survivin siRNA and oridonin

Journal of Colloid and Interface Science, Journal Year: 2023, Volume and Issue: 646, P. 370 - 380

Published: April 28, 2023

In recent years, small interfering RNA (siRNA) has been widely used in the treatment of human diseases, especially tumors, and shown great appeal. However, clinical application siRNA faces several challenges. Insufficient efficacy, poor bioavailability, stability, lack responsiveness to a single therapy are main problems affecting tumor therapy. Here, we designed cell-penetrating peptide (CPP)-modified metal organic framework nanoplatform (named PEG-CPP33@ORI@survivin siRNA@ZIF-90, PEG-CPP33@NPs) for targeted co-delivery oridonin (ORI), natural anti-tumor active ingredient) survivin vivo. This can improve stability bioavailability efficacy monotherapy. The high drug-loading capacity pH-sensitive properties zeolite imidazolides endowed PEG-CPP33@NPs with lysosomal escape abilities. Polyethylene glycol (PEG)-conjugated CPP (PEG-CPP33) coating significantly improved uptake vitro results showed that ORI greatly enhanced effect PEG-CPP33@NPs, demonstrating synergistic between siRNA. summary, novel nanobiological platform loaded presented herein advantages cancer therapy, provides an attractive strategy chemotherapy gene

Language: Английский

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Incorporating copper-based nanosheets into an injectable self-healing hydrogel enables superb repair of infected diabetic wound

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 476, P. 146788 - 146788

Published: Oct. 20, 2023

Language: Английский

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Fe₃O₄‐Incorporated Metal‐Organic Framework for Chemo/Ferroptosis Synergistic Anti‐Tumor via the Enhanced Chemodynamic Therapy

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(14)

Published: Feb. 9, 2024

Metal-organic framework (MOF)-based drug delivery nanomaterials for cancer therapy have attracted increasing attention in recent years. Here, an enhanced chemodynamic anti-tumor strategy by promoting the Fenton reaction using core-shell zeolitic imidazolate framework-8 (ZIF-8)@Fe

Language: Английский

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ZnO‐CuS/F127 Hydrogels with Multienzyme Properties for Implant‐Related Infection Therapy by Inhibiting Bacterial Arginine Biosynthesis and Promoting Tissue Repair

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 16, 2024

Abstract Implant‐related infections are characterized by the formation of bacterial biofilms. Current treatments have various drawbacks. Nanozymes with enzyme‐like activity can produce highly toxic substances to kill bacteria and remove biofilms without inducing drug resistance. However, it is difficult for current monometallic nanozymes function well in complex biofilm environments. Therefore, development multimetallic efficient multienzyme activities crucial. In present study, bimetallic nanozyme, ZnO‐CuS nanoflowers peroxidase (POD), glutathione oxidase (GSH‐Px), catalase (CAT) successfully synthesized via calcination loaded into F127 hydrogels treatment implant‐related infections. The ability bind key antimicrobial activity. addition, H 2 O disrupt metabolism MRSA , including arginine synthesis, nucleotide excision repair, energy metabolism, protein synthesis. ZnO‐CuS/F127 hydrogel combination has been demonstrated be effective clearing infection facilitating switch M1 macrophages M2‐repairative phenotype implant mice. Furthermore, favorable biosafety, their toxicity negligible. provided a promising biomedical strategy healing infections, highlighting potential clinical applications.

Language: Английский

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Composite hydrogels fabricated from CMC-PVA-GG incorporated with ZiF-8 for wound healing applications

Journal of Biomaterials Science Polymer Edition, Journal Year: 2024, Volume and Issue: 35(17), P. 2640 - 2659

Published: Aug. 7, 2024

The skin is at risk for injury to external factors since it serves as the body's first line of defense against environment. Hydrogels have drawn much interest due their intrinsic extracellular matrix (ECM) properties and biomimetic, structural, durable mechanical characteristics. enormous potential use in wound healing ability deliver bioactive substances easily. In this study, composite hydrogels were developed by blending guar gum (GG), polyvinyl alcohol (PVA), carboxymethyl cellulose (CMC) with crosslinker TEOS treatment. surface morphology, roughness, stability features characterized several techniques, such FTIR, SEM-EDX, AFM, DSC. increasing ZiF-8 causes more decreased swelling different media (Aqueous > PBS NaCl). amount less hydrophilic behavior biodegradation gel fraction. cytocompatibility Zinc imidazolate framework-8 (ZiF-8) based composites was evaluated fibroblast cell lines viability, proliferation, morphology. caused viability proliferation proper Hence, results show that synthesized may be a candidate numerous repair applications.

Language: Английский

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Double‐Edged Dissolving Microneedle Patches Loaded with Zn/Ce Composites and Vancomycin for Treatment of Drug‐Resistant Bacterial Infected Skin Abscess

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 16, 2025

The management of abscess wounds induced by antibiotic-resistant bacterial infections has become increasingly formidable due to the widespread overutilization and misuse antimicrobial agents. This study presents an innovative dissolvable microneedle (MN) patch incorporating Au@ZnO/Ce nanocomposites vancomycin (AZC/Van@MN), exhibiting robust anti-inflammatory properties, meticulously engineered for therapeutic intervention wounds. developed AZC/Van@MN demonstrates exceptional biocompatibility as evidenced comprehensive histopathological hematological assessments. It effectively eradicates colonies through synergistic action Van mild photothermal therapy (PTT, ≤42 °C). Transcriptomic analysis elucidates that antibacterial mechanism involves upregulation riboflavin biosynthesis suppression arginine pathways. Furthermore, significantly reduces dimensions, load, inflammatory response, while simultaneously enhancing wound healing via accelerated re-epithelialization angiogenesis. double-edged MN represents a promising strategy combating skin abscesses instigated bacteria.

Language: Английский

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Synthesis of γ-Cyclodextrin-Reduced Fe(III) Nanoparticles with Peroxidase-like Catalytic Activity for Bacteriostasis of Food

Nano Letters, Journal Year: 2023, Volume and Issue: 23(21), P. 9995 - 10003

Published: Oct. 19, 2023

Foodborne pathogens are a primary cause of human foodborne illness, making it imperative to explore novel antibacterial strategies for their control. In this study, Fe-γ-CD was successfully synthesized as food agent use in milk and orange juice. The consists 6/11 Fe(II) 5/11 Fe(III), which catalyze Fenton-like catalytic reaction with H2O2 generate •OH. Consequently, exhibits exceptional peroxidase-like activity broad-spectrum efficacy. not only disrupts the wall structure ESBL-E. coli but also induces protein leakage genetic destruction, ultimately leading its death. Furthermore, inhibits biofilm formation by MRSA eradicates mature biofilms, resulting MRSA's demise. Importantly, demonstrates negligible cytotoxicity toward normal mammalian cells, an ideal candidate application foodstuffs. These findings highlight that is effective tool combating spread safety.

Language: Английский

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