Investigation of multi-drug resistant Candida auris using species-specific molecular markers in immunocompromised patients from a tertiary care hospital in Quetta, Pakistan DOI Creative Commons

Hira Ejaz,

Muhammad Mushtaq,

Shereen Khan

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0319485 - e0319485

Published: April 24, 2025

Introduction Candida auris is an emerging multidrug-resistant pathogen responsible for nosocomial infections worldwide, characterized by high mortality rates and significant challenges in detection due to frequent misidentification. Classified the WHO as a of critical importance since it exhibits resistance multiple antifungal agents, particularly fluconazole, highly transmissible healthcare settings. Conventional methods often lack accuracy required effective infection control. This study aimed conduct inferential molecular analyses C. other yeast species infecting immunocompromised patients Special Intensive Care Units (SCU ICU) tertiary care hospital Quetta, Pakistan. In this region, remains rarely studied frequently misdiagnosed clinical staff limited awareness diagnostic challenges. Notably, no prior research has been conducted on Quetta. The also sought develop reliable suitable resource-limited settings, addressing gap infrastructure. Materials Samples (150 each) from ear, axilla, groin, saliva SCU/ICU were collected processed malt agar, with preliminary identification using Brilliance Agar (BCA) CHROMagar Plus (CCP). Advanced techniques, including PCR amplification ITS regions, DNA sequencing, RFLP Msp 1, MALDI-TOF, Vitek 2, species-specific primers, used identification. Antifungal susceptibility amphotericin B, voriconazole assessed. Results culture test revealed that 42.6% samples positive infections. addition detecting 4 cultures, chromogenic media identified 6 species: albicans , dubliniensis glabrata krusei parapsilosis tropicalis . Further validation through advanced diagnostics enabled additional famata kefyr lusitaniae Meyerozyma ( ) guilliermondii Out all was most common, followed highest observed samples. Susceptibility Tests (AST) isolates resistant Fluconazole, Amphotericin Voriconazole, highlighting multidrug resistance. represents first report novel Pakistan, indicating prevalent among ICU SCU patients. Novel specific primers targeting phospholipase topoisomerase II, CDR 18s genes designed our laboratory not previously reported earlier studies, proved rapid species. established protocol these recommended implementation statistical analysis demonstrated correlations between (dependent variable) several independent factors (variables) emphasizing targeted intervention strategies.

Language: Английский

Outline of Fungi and fungus-like taxa – 2021 DOI Creative Commons

NN Wijayawardene,

KD Hyde,

DQ Dai

et al.

Mycosphere, Journal Year: 2022, Volume and Issue: 13(1), P. 53 - 453

Published: Jan. 1, 2022

This paper provides an updated classification of the Kingdom Fungi (including fossil fungi) and fungus-like taxa.Five-hundred twenty-three (535) notes are provided for newly introduced taxa changes that have been made since previous outline.In discussion, latest taxonomic in Basidiomycota Mycosphaerellales broadly discussed.Genera listed Mycosphaerellaceae confirmed by DNA sequence analyses, while doubtful genera (DNA sequences being unavailable but traditionally accommodated Mycosphaerellaceae) discussion.Problematic Glomeromycota also discussed based on phylogenetic results.

Language: Английский

Citations

410
What are fungal species and how to delineate them? DOI
K. W. Thilini Chethana, Ishara S. Manawasinghe, Vedprakash G. Hurdeal

et al.

Fungal Diversity, Journal Year: 2021, Volume and Issue: 109(1), P. 1 - 25

Published: July 1, 2021

Language: Английский

Citations

170
A genome-informed higher rank classification of the biotechnologically important fungal subphylum Saccharomycotina DOI Creative Commons
Marizeth Groenewald, Chris Todd Hittinger,

Konstanze Bensch

et al.

Studies in Mycology, Journal Year: 2023, Volume and Issue: 105(1), P. 1 - 22

Published: May 26, 2023

Language: Английский

Citations

57
Current trends, limitations and future research in the fungi? DOI Creative Commons
Kevin D. Hyde, Petr Baldrián, Yanpeng Chen

et al.

Fungal Diversity, Journal Year: 2024, Volume and Issue: 125(1), P. 1 - 71

Published: March 20, 2024

Abstract The field of mycology has grown from an underappreciated subset botany, to a valuable, modern scientific discipline. As this study grown, there have been significant contributions science, technology, and industry, highlighting the value fungi in era. This paper looks at current research, along with existing limitations, suggests future areas where scientists can focus their efforts, mycology. We show how become important emerging diseases medical discuss trends potential drug novel compound discovery. explore phylogenomics, its potential, outcomes address question phylogenomics be applied fungal ecology. In addition, functional genomics studies are discussed importance unravelling intricate mechanisms underlying behaviour, interactions, adaptations, paving way for comprehensive understanding biology. look research building materials, they used as carbon sinks, biocircular economies. numbers always great interest often written about estimates varied greatly. Thus, we needs order obtain more reliable estimates. aspects machine learning (AI) it mycological research. Plant pathogens affecting food production systems on global scale, such, needed area, particularly disease detection. latest data High Throughput Sequencing if still gaining new knowledge same rate before. A review nanotechnology is provided addressed. Arbuscular Mycorrhizal Fungi addressed acknowledged. Fungal databases becoming important, therefore provide major databases. Edible medicinal huge medicines, especially Asia prospects discussed. Lifestyle changes (e.g., endophytes, pathogens, and/or saprobes) also extremely trend special issue Diversity.

Language: Английский

Citations

30
Trends in yeast diversity discovery DOI Creative Commons

Teun Boekhout,

Anthony S. Amend, Fouad El Baidouri

et al.

Fungal Diversity, Journal Year: 2021, Volume and Issue: 114(1), P. 491 - 537

Published: Dec. 3, 2021

Abstract Yeasts, usually defined as unicellular fungi, occur in various fungal lineages. Hence, they are not a taxonomic unit, but rather represent lifestyle shared by several unrelated Although the discovery of new yeast species occurs at an increasing speed, current rate it will likely take hundreds years, if ever, before all be documented. Many parts earth, including many threatened habitats, remain unsampled for yeasts and others only superficially studied. Cold such glaciers, home to specific community cold-adapted yeasts, and, hence, there is some urgency study environments locations where might disappear soon due anthropogenic climate change. The same true communities natural forests that impacted deforestation forest conversion. countries so-called Global South have been sampled despite their economic promise. However, extensive research activity Asia, especially China, has yielded novelties. Comparative genomics studies demonstrated presence with hybrid origin, them isolated from clinical or industrial environments. DNA-metabarcoding prevalence, cases dominance, soils marine waters worldwide, surprising distributions, unexpected common Malassezia habitats.

Language: Английский

Citations

80
Ten decadal advances in fungal biology leading towards human well-being DOI Creative Commons
Ausana Mapook,

K. D. Hyde,

Khadija Hassan

et al.

Fungal Diversity, Journal Year: 2022, Volume and Issue: 116(1), P. 547 - 614

Published: Sept. 1, 2022

Fungi are an understudied resource possessing huge potential for developing products that can greatly improve human well-being. In the current paper, we highlight some important discoveries and developments in applied mycology interdisciplinary Life Science research. These examples concern recently introduced drugs treatment of infections neurological diseases; application -OMICS techniques genetic tools medical regulation mycotoxin production; as well highlights mushroom cultivaton Asia. Examples new diagnostic exploitation candidates therapeutic drugs, also given. addition, two entries illustrating latest use fungi biodegradation fungal biomaterial production provided. Some other areas where there have been and/or will be significant included. It is our hope this paper help realise importance a industrial see next decades bring forward many fungus-derived products.

Language: Английский

Citations

51
Forecasting the number of species of asexually reproducing fungi (Ascomycota and Basidiomycota) DOI
Nalin N. Wijayawardene, Alan J. L. Phillips, Diana S. Pereira

et al.

Fungal Diversity, Journal Year: 2022, Volume and Issue: 114(1), P. 463 - 490

Published: March 13, 2022

Language: Английский

Citations

40
Yeasts from temperate forests DOI
Simone Mozzachiodi, Feng‐Yan Bai, Petr Baldrián

et al.

Yeast, Journal Year: 2022, Volume and Issue: 39(1-2), P. 4 - 24

Published: Jan. 1, 2022

Yeasts are ubiquitous in temperate forests. While this broad habitat is well-defined, the yeasts inhabiting it and their life cycles, niches, contributions to ecosystem functioning less understood. present on nearly all sampled substrates forests worldwide. They associate with soils, macroorganisms, other habitats no doubt contribute broader ecosystem-wide processes. Researchers have gathered information leading hypotheses about yeasts' niches cycles based physiological observations laboratory as well genomic analyses, but challenge remains test these themselves. Here, we summarize global patterns of yeast diversity, give some a handful well-studied forest genera, discuss various strategies isolate yeasts, explain biotechnology. We close summary many future directions outstanding questions facing researchers ecology. an exciting opportunity better understand hidden world microbial ecology threatened habitat.

Language: Английский

Citations

39
Macroevolutionary diversity of traits and genomes in the model yeast genus Saccharomyces DOI Creative Commons
David Peris, Emily J. Ubbelohde, Meihua Christina Kuang

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Feb. 8, 2023

Abstract Species is the fundamental unit to quantify biodiversity. In recent years, model yeast Saccharomyces cerevisiae has seen an increased number of studies related its geographical distribution, population structure, and phenotypic diversity. However, seven additional species from same genus have been less thoroughly studied, which limited our understanding macroevolutionary events leading diversification this over last 20 million years. Here, we show geographies, hosts, substrates, phylogenetic relationships for approximately 1,800 strains, covering complete with unprecedented breadth depth. We generated analyzed genome sequences 163 strains phenotyped 128 phylogenetically diverse strains. This dataset provides insights about genetic diversity within between populations, quantifies reticulation incomplete lineage sorting, demonstrates how gene flow selection affected traits, such as galactose metabolism. These findings elevate a understand biodiversity evolution in microbial eukaryotes.

Language: Английский

Citations

30
Proposal of one new family, seven new genera and seventy new basidiomycetous yeast species mostly isolated from Tibet and Yunnan provinces, China DOI

Yu-Long Jiang,

Wen-Qing Bao, Fang Liu

et al.

Studies in Mycology, Journal Year: 2024, Volume and Issue: 109(1), P. 57 - 154

Published: June 28, 2024

Language: Английский

Citations

9