Quercetin Protects Against Global Cerebral ischemia‒reperfusion Injury by Inhibiting Microglial Activation and Polarization

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 1281 - 1293

Published: Feb. 1, 2024

Background: This study aims to investigate the protective effect of quercetin against global cerebral ischemia‒reperfusion (GCI/R) injury in rats and elucidate underlying mechanism. Methods: A GCI/R rat model was established using a four-vessel occlusion (4-VO) method. An oxygen–glucose deprivation/reoxygenation (OGD/R) induced BV2 cells. The extent assessed by evaluating neurological deficit scores (NDS) brain water content conducting behavioral tests. Pathomorphological changes prefrontal cortex were examined. Additionally, measured levels inflammatory cytokines, degree microglial activation polarization, protein expression Toll-like receptor 4 (TLR4) TIR-domain-containing adaptor inducing interferon-β (TRIF). Results: Quercetin pretreatment significantly ameliorated impairment, improved learning memory abilities, reduced anxiety subjected injury. Furthermore, administration effectively mitigated neuronal edema. Notably, it suppressed hindered polarization toward M1 phenotype. Simultaneously, downregulated TLR4 TRIF proteins attenuated release IL-1β TNF-α. Conclusion: highlights novel therapeutic potential alleviating demonstrates its neuroprotective effects inhibiting neuroinflammation while impeding their transformation into phenotype through modulation TLR4/TRIF pathway. Keywords: quercetin, ischemia, neuroprotection,

Language: Английский

---

LILRB2 inhibition enhances radiation sensitivity in non-small cell lung cancer by attenuating radiation-induced senescence

Cancer Letters, Journal Year: 2024, Volume and Issue: 593, P. 216930 - 216930

Published: May 3, 2024

Radiotherapy (RT) in non-small cell lung cancer (NSCLC) triggers cellular senescence, complicating tumor microenvironments and affecting treatment outcomes. This study examines the role of lymphocyte immunoglobulin-like receptor B2 (LILRB2) modulating RT-induced senescence radiosensitivity NSCLC. Through methodologies including irradiation, lentivirus transfection, various molecular assays, we assessed LILRB2's expression its impact on levels behaviors. Our findings reveal that RT upregulates LILRB2, facilitating a senescence-associated secretory phenotype (SASP), which turn enhances proliferation resistance to radiation. Importantly, LILRB2 silencing attenuates these effects by inhibiting JAK2/STAT3 pathway, significantly increasing NSCLC models. Clinical data correlate high with reduced response poorer prognosis, suggesting pivotal potential as therapeutic target improve radiosensitivity.

Language: Английский

---

Zhen-wu-tang protects against myocardial fibrosis by inhibiting M1 macrophage polarization via the TLR4/NF-κB pathway

Phytomedicine, Journal Year: 2024, Volume and Issue: 130, P. 155719 - 155719

Published: May 9, 2024

Language: Английский

---

Gelatin methacryloyl microneedle loaded with 3D-MSC-Exosomes for the protection of ischemia-reperfusion

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 275, P. 133336 - 133336

Published: June 25, 2024

Language: Английский

---

Quercetin restores respiratory mucosal barrier dysfunction in Mycoplasma gallisepticum-infected chicks by enhancing Th2 immune response

Phytomedicine, Journal Year: 2024, Volume and Issue: 133, P. 155953 - 155953

Published: Aug. 12, 2024

Language: Английский

---

Unlocking the Potential: Quercetin and Its Natural Derivatives as Promising Therapeutics for Sepsis

Biomedicines, Journal Year: 2024, Volume and Issue: 12(2), P. 444 - 444

Published: Feb. 16, 2024

Sepsis is a syndrome of organ dysfunction caused by an uncontrolled inflammatory response, which can seriously endanger life. Currently, there still shortage specific therapeutic drugs. Quercetin and its natural derivatives have received lot attention recently for their potential in treating sepsis. Here, we provide comprehensive summary the recent research progress on quercetin derivatives, with focus mechanisms antioxidation anti-inflammation. To obtain necessary information, conducted search PubMed, Web Science, EBSCO, Cochrane library databases using keywords sepsis, anti-inflammatory, antioxidant, anti-infection, quercetin, to identify relevant from 6315 articles published last five years. At present, 11 been intensively studied. They primarily exert anti-inflammation effects through PI3K/AKT/NF-κB, Nrf2/ARE, MAPK pathways. The feasibility these compounds experimental models clinical application were also discussed. In conclusion, good treatment

Language: Английский

---

Apilarnil exerts neuroprotective effects and alleviates motor dysfunction by rebalancing M1/M2 microglia polarization, regulating miR-155 and miR-124 expression in a rotenone-induced Parkinson’s disease rat model

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 137, P. 112536 - 112536

Published: June 22, 2024

Language: Английский

---

Neuroprotective Effects of Eugenol Acetate Against Ischemic Stroke

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 133 - 146

Published: Jan. 1, 2025

To explore the neuroprotective effect of Eugenol Acetate (EA) on post-stroke neuroinflammation and investigate underlying mechanisms. For in vitro experiments, primary microglia were pre-incubated with EA for 2 hours, followed by lipopolysaccharide (LPS) stimulation 24 hours or Oxygen-Glucose Deprivation (OGD) treatment 4 hours. Real-time quantitative PCR, enzyme-linked immunosorbent assay (ELISA) Western blot performed to examine expression levels inflammatory cytokines microglia. The activation NF-κB signaling pathway was evaluated immunofluorescence staining blot. vivo middle cerebral artery occlusion (MCAO) constructed mimic ischemic brain injury 8-week-old male C57BL/6J mice. mice continuously injected intraperitoneally vehicle after MCAO. Neurobehavioral tests TTC conducted estimate neurological deficits infarct area. Moreover, white matter integrity MCAO observed via staining. significantly reduced pro-inflammatory LPS OGD treated microglia, inhibited LPS-induced pathway. In addition, alleviated improved neuromotor function Furthermore, long-term restored mice, which might be associated inhibition Therefore, a promising candidate stroke.

Language: Английский

---

Curcumin Regulates Microglia Polarization to Alleviate Ischemic Stroke by Targeting microRNA‐205‐5p/Kruppel‐Like Factor 2 (KLF2)/Activating Transcription Factor 2 (ATF2) Axis

Chemical Biology & Drug Design, Journal Year: 2025, Volume and Issue: 105(1)

Published: Jan. 1, 2025

ABSTRACT Ischemic stroke (IS) often causes fearful sequela, even death. Curcumin was beneficial to IS, but its underlying molecular mechanism is unclear. Mice were subjected middle cerebral artery occlusion (MCAO) surgery, and BV‐2 cells treated with oxygen–glucose deprivation/reoxygenation (OGD/R) induction establish IS models in vivo vitro. Abundance of genes proteins determined using quantitative real‐time polymerase chain reaction (RT‐qPCR), immunofluorescence (IF), western blot. Interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), interleukin‐10 (IL‐10) levels analyzed enzyme‐linked immunosorbent assay (ELISA). Modified neurological severity score (mNSS), corner test, foot fault adhesive removal 2,3,5‐triphenyltetrazolium chloride (TTC) staining applied evaluate the brain injury mice. The correlation between miR‐205‐5p Kruppel‐like factor 2 (KLF2) affirmed dual luciferase reporter assay. Our results revealed that curcumin alleviated damage MCAO mice through driving microglia M2 polarization. Of note, resulted decreased expression knockdown promoted polarization OGD/R conditions achieved similar treatment Moreover, played a promoting role under conditions, while overexpression or KLF2 abolished these effects. On mechanism, target curcumin, further interacted inhibit activating transcription (ATF2) expression. miR‐205‐5p, by suppressed worsen mediating KLF2/ATF2 axis.

Language: Английский

---

Multi-targeted nanogel drug delivery system alleviates neuroinflammation and promotes spinal cord injury repair

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101518 - 101518

Published: Jan. 23, 2025

Spinal cord injury (SCI) is significantly hampered by an inflammatory microenvironment, prompting continued efforts in drug development to address inflammation. Research shows that quercetin (Que) exhibits excellent performance reducing inflammation and neuroprotection. However, its application limited poor solubility, notable side effects, the unique pathophysiology of spinal cord. In this study, we introduce a novel multifunctional liposome hydrogel delivery system (QLipTC@HDM), obtained incorporating liposomes with blood-spinal barrier penetration site targeting properties (LipTC) into dual-network viscous (HDM). Our results demonstrate encapsulating Que LipTC (QLipTC) enhances minimizes toxic facilitates lesion targeting, aids crossing barrier. Moreover, encapsulation HDM prolongs retention QLipTC at after local administration. Crucially, our findings reveal QLipTC@HDM induces M2 phenotype transformation glial cells mice SCI, thereby mitigating This intervention additionally preserves integrity barrier, optimizes reduces scarring, promotes axonal regeneration, motor function recovery SCI mice. summary, investigations highlight potential disease-specific as promising therapeutic approach for treatment management SCI.

Language: Английский

---