An assay for chemical nociception inDrosophilalarvae DOI Creative Commons
Roger Lopez-Bellido, Nathaniel J. Himmel, Howard B. Gutstein

et al.

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2019, Volume and Issue: 374(1785), P. 20190282 - 20190282

Published: Sept. 23, 2019

Chemically induced nociception has not yet been studied intensively in genetically tractable models. Hence, our goal was to establish a Drosophila assay that can be used study the cellular and molecular/genetic bases of chemically nociception. larvae exposed increasing concentrations hydrochloric acid (HCl) produced an increasingly intense aversive rolling response. HCl (0.5%) subthreshold provoked no All classes peripheral multidendritic (md) sensory neurons (classes I–IV) are required for full responsiveness acid, with class IV making largest contribution. At level, IV, III I showed increases calcium following exposure. In central nervous system, Basin-4 second-order key regulators nociception, slight contribution from other types. Finally, chemical sensitized by tissue damage. Subthreshold allodynia 4 h after physical puncture wounding. Pinch wounding UV irradiation, which do compromise cuticle, did cause allodynia. sum, we developed novel larvae. This assay, combined high genetic resolving power Drosophila, should improve basic understanding fundamental mechanisms article is part Theo Murphy meeting issue ‘Evolution behaviour important pain’.

Language: Английский

Molecular sensors in the taste system of Drosophila DOI
Bhanu Shrestha, Youngseok Lee

Genes & Genomics, Journal Year: 2023, Volume and Issue: 45(6), P. 693 - 707

Published: Feb. 24, 2023

Language: Английский

Citations

23
Taste coding of heavy metal ion-induced avoidance in Drosophila DOI Creative Commons
Xiaonan Li, Yuanjie Sun, Shan Gao

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(5), P. 106607 - 106607

Published: April 7, 2023

Increasing pollution of heavy metals poses great risks to animals globally. Their survival likely relies on an ability detect and avoid harmful metal ions (HMIs). Currently, little is known about the neural mechanisms HMI detection. Here, we show that Drosophila related species Drosophilidae actively toxic HMIs at micromolar concentrations. The high sensitivity biologically relevant. Particularly, their cadmium as most bitter substance, denatonium. Detection in food requires Gr66a+ gustatory neurons but independent bitter-taste receptors. In these neurons, ionotropic receptors IR76b, IR25a, IR7a are required for perception metals. Furthermore, IR47a mediates activation a distinct group non-Gr66a+ elicited by HMIs. Together, our findings reveal surprising taste quality represented noxious ions.

Language: Английский

Citations

20
Molecular Basis of Hexanoic Acid Taste in Drosophila melanogaster DOI Open Access
Roshani Nhuchhen Pradhan, Bhanu Shrestha, Youngseok Lee

et al.

Molecules and Cells, Journal Year: 2023, Volume and Issue: 46(7), P. 451 - 460

Published: May 19, 2023

Animals generally prefer nutrients and avoid toxic harmful chemicals.Recent behavioral physiological studies have identified that sweet-sensing gustatory receptor neurons (GRNs) in Drosophila melanogaster mediate appetitive behaviors toward fatty acids.Sweet-sensing GRN activation requires the function of ionotropic receptors IR25a, IR56d, IR76b, as well GR64e.However, we reveal hexanoic acid (HA) is rather than nutritious to D. melanogaster.HA one major components fruit Morinda citrifolia (noni).Thus, analyzed responses noni acids, HA, via electrophysiology proboscis extension response (PER) assay.Electrophysiological tests show this reminiscent arginine-mediated neuronal responses.Here, determined a low concentration HA induced attraction, which was mediated by GRNs, high aversion, bitter-sensing GRNs.We also demonstrated elicits attraction mainly GR64d IR56d expressed but activates three (GR32a, GR33a, GR66a) GRNs.The mechanism sensing biphasic dose dependent manner.Furthermore, inhibit sugar-mediated like other bitter compounds.Taken together, discovered binary HA-sensing may be evolutionarily meaningful foraging niche insects.

Language: Английский

Citations

20
Alkaline taste sensation through the alkaliphile chloride channel in Drosophila DOI
Ting‐Wei Mi, J. Mack,

Wyatt Koolmees

et al.

Nature Metabolism, Journal Year: 2023, Volume and Issue: 5(3), P. 466 - 480

Published: March 20, 2023

Language: Английский

Citations

18
A single pair of pharyngeal neurons functions as a commander to reject high salt in Drosophila melanogaster DOI Creative Commons
Jiun Sang, Subash Dhakal, Bhanu Shrestha

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Dec. 27, 2023

Salt (NaCl), is an essential nutrient for survival, while excessive salt can be detrimental. In the fruit fly, Drosophila melanogaster , internal taste organs in pharynx are critical gatekeepers impacting decision to accept or reject a food. Currently, our understanding of mechanism through which pharyngeal gustatory receptor neurons (GRNs) sense high rudimentary. Here, we found that member ionotropic family, Ir60b expressed exclusively pair GRNs activated by salt. Using two-way choice assay (DrosoX) measure ingestion volume, demonstrate IR60b and two co-receptors IR25a IR76b required prevent consumption. Mutants lacking external but retaining exhibit much higher avoidance than flies with all missing three IRs. Our findings highlight vital role IRs GRN control

Language: Английский

Citations

17
Avoiding alkaline taste through ionotropic receptors DOI Creative Commons
Prakash Pandey,

Bhanu Shrestha,

Youngseok Lee

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 110087 - 110087

Published: May 23, 2024

Taste organs contain distinct gustatory receptors that help organisms differentiate between nourishing and potentially harmful foods. The detection of high pH levels plays a crucial role in food selection, but the specific responsible for perceiving elevated foods have remained unknown. By using

Language: Английский

Citations

7
Genomic identification and evolutionary analysis of chemosensory receptor gene families in two Phthorimaea pest species: insights into chemical ecology and host adaptation DOI Creative Commons
Ruipeng Chen, Junjie Yan, Jacob D. Wickham

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: May 18, 2024

Abstract Background Insects rely on sophisticated sensitive chemosensory systems to sense their complex chemical environment. This sensory process involves a combination of odorant receptors (ORs), gustatory (GRs) and ionotropic (IRs) in the system. study focused identification characterization these three types receptor genes two closely related Phthorimaea pest species, operculella (potato tuber moth) absoluta (tomato leaf miner). Results Based manual annotation genome, we identified total 349 chemoreceptor from genome P. , including 93 OR 206 GR 50 IR genes, while for absoluta, 72 122 46 genes. Through phylogenetic analysis, observed minimal differences number ORs IRs between potato moth tomato miner. In addition, found that compared with those miners, branch has undergone large expansion, which may be having narrower host range than . analysis differentially expressed (DEGs) male female antennae, uncovered 45 DEGs (including 32ORs, 9 GRs, 4 IRs). Conclusions Our research provides foundation exploring ecology pests offers new insights into dietary differentiation lepidopteran insects, simultaneously providing molecular targets developing environmentally friendly control methods based insect chemoreception.

Language: Английский

Citations

6
Molecular and cellular basis of acid taste sensation in Drosophila DOI Creative Commons
Ting‐Wei Mi, J. Mack, Christopher M. Lee

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: June 17, 2021

Abstract Acid taste, evoked mainly by protons (H + ), is a core taste modality for many organisms. The hedonic valence of acid bidirectional: animals prefer slightly but avoid highly acidic foods. However, how discriminate low from high acidity remains poorly understood. To explore the perception acid, we use fruit fly as model organism. We find that flies employ two competing sensory pathways to detect and acidity, relative degree activation each determines either attractive or aversive responses. Moreover, establish one member Otopetrin family, Otopetrin-like (OtopLa), proton channel dedicated gustatory detection acid. OtopLa defines unique subset receptor neurons selectively required rather than Loss otopla causes reject normally low-acid Therefore, identification sensor firmly supports our competition sensation. Altogether, have discovered binary acid-sensing mechanism may be evolutionarily conserved between insects mammals.

Language: Английский

Citations

41
Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer’s disease DOI Creative Commons
Suman Rimal, Yu Li, Rasika Vartak

et al.

Acta Neuropathologica Communications, Journal Year: 2021, Volume and Issue: 9(1)

Published: Oct. 18, 2021

Abstract Amyloid precursor protein (APP) metabolism is central to Alzheimer’s disease (AD) pathogenesis, but the key etiological driver remains elusive. Recent failures of clinical trials targeting amyloid-β (Aβ) peptides, proteolytic fragments amyloid that are main component plaques, suggest proteostasis-disrupting, pathogenic species remain be identified. Previous studies APP C-terminal fragment (APP.C99) can cause in an Aβ-independent manner. The mechanism APP.C99 pathogenesis incompletely understood. We used Drosophila models expressing with native ER-targeting signal human APP, full-length only, or co-expressing and β-secretase (BACE), investigate mechanisms pathogenesis. Key findings validated mammalian cell culture models, mouse 5xFAD model, postmortem AD patient brain materials. find ribosomes stall at ER membrane during co-translational translocation APP.C99, activating ribosome-associated quality control (RQC) resolve ribosome collision stalled translation. Stalled extensions (CAT-tails) resulting from inadequate RQC prone aggregation, causing endolysosomal autophagy defects seeding aggregation β plaques. Genetically removing CAT-tailed rescued proteostasis failure, endolysosomal/autophagy dysfunction, neuromuscular degeneration, cognitive deficits models. Our finding factor deposition core plaques brains further supports role defective translation These demonstrate plaque formation consequence manifestation a deeper level failure caused by translational stalling resultant aberrantly modified species, previously unrecognized drivers newly discovered therapeutic targets.

Language: Английский

Citations

41
Requirement for an Otopetrin-like protein for acid taste in Drosophila DOI Creative Commons
Anindya Ganguly, Avinash Chandel, Heather N. Turner

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2021, Volume and Issue: 118(51)

Published: Dec. 15, 2021

Significance The taste of acids is critical for animal survival since it enables them to differentiate potentially dangerous from nutritious foods. Due the general requirement acid survival, we tested idea that receptor mechanism functioning in may be evolutionarily conserved. Here, demonstrate mutation a Drosophila gene, Otopetrin-Like A ( OtopLA ), encoding protein distantly related recently identified mammalian receptor, OTOP1, essential both strong repulsion highly acidic food and mild attraction low acidity. aversion requires expression distinct neurons fly equivalent vertebrate tongue.

Language: Английский

Citations

35