Biomarkers for the adverse effects on respiratory system health associated with atmospheric particulate matter exposure

Journal of Hazardous Materials, Journal Year: 2021, Volume and Issue: 421, P. 126760 - 126760

Published: July 27, 2021

Language: Английский

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The toxicity of nanoparticles and their interaction with cells: an in vitro metabolomic perspective

Nanoscale Advances, Journal Year: 2023, Volume and Issue: 5(10), P. 2674 - 2723

Published: Jan. 1, 2023

The interaction of nanoparticles (NPs) with cells depends on their physicochemical properties and can lead to cytotoxic events. Metabolomics reveals the molecular mechanisms this nanotoxicity avoids misleading interferences NPs.

Language: Английский

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Silica nanoparticles aggravated the metabolic associated fatty liver disease through disturbed amino acid and lipid metabolisms-mediated oxidative stress

Redox Biology, Journal Year: 2022, Volume and Issue: 59, P. 102569 - 102569

Published: Dec. 5, 2022

The metabolic associated fatty liver disease (MAFLD) is a public health challenge, leading to global increase in chronic disease. respiratory exposure of silica nanoparticles (SiNPs) has revealed induce hepatotoxicity. However, its role the pathogenesis and progression MAFLD was severely under-studied. In this context, hepatic impacts SiNPs were investigated vivo vitro through using ApoE-/- mice free acid (FFA)-treated L02 hepatocytes. Histopathological examinations biochemical analysis showed via intratracheal instillation aggravated steatosis, lipid vacuolation, inflammatory infiltration even collagen deposition mice, companied with increased ALT, AST LDH levels. enhanced synthesis inhibited β-oxidation efflux may account for TC/TG by SiNPs. Consistently, induced elevated TC FFA-treated cells. Further, activation oxidative stress detected vitro, as evidenced ROS accumulation, MDA, declined GSH/GSSG down-regulated Nrf2 signaling. Endoplasmic reticulum (ER) also triggered response SiNPs-induced reflecting remarkable ER expansion BIP expression. More importantly, an UPLC-MS-based metabolomics that disturbed profile prominently on amino acids metabolisms. particular, identified differential metabolites strongly correlated ensuing accumulation injuries, contributing diseases. Taken together, our study promoted steatosis damage, resulting aggravation progression. metabolisms-mediated key contributor phenomenon from perspective.

Language: Английский

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Nanotechnology-based mRNA vaccines

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: Aug. 17, 2023

Language: Английский

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A toxicological profile of silica nanoparticles

Toxicology Research, Journal Year: 2022, Volume and Issue: 11(4), P. 565 - 582

Published: July 16, 2022

Abstract Humans are regularly exposed to silica nanoparticles in environmental and occupational contexts, these exposures have been implicated the onset of adverse health effects. Existing reviews on nanoparticle toxicity few not comprehensive. There natural synthetic sources by which crystalline amorphous produced. These processes influence physiochemical properties, factors that can dictate toxicological Toxicological assessment includes exposure scenario (e.g. environmental, occupational), route exposure, toxicokinetics, toxicodynamics. Broader considerations include pathology, risk assessment, regulation, treatment after injury. This review aims consolidate most relevant up-to-date research areas provide an exhaustive profile nanoparticles.

Language: Английский

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Prospects and hazards of silica nanoparticles: Biological impacts and implicated mechanisms

Biotechnology Advances, Journal Year: 2023, Volume and Issue: 69, P. 108277 - 108277

Published: Nov. 3, 2023

Language: Английский

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Nanozyme as a rising star for metabolic disease management

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 6, 2024

Abstract Nanozyme, characterized by outstanding and inherent enzyme-mimicking properties, have emerged as highly promising alternatives to natural enzymes owning their exceptional attributes such regulation of oxidative stress, convenient storage, adjustable catalytic activities, remarkable stability, effortless scalability for large-scale production. Given the potent regulatory function nanozymes on stress coupled with fact that reactive oxygen species (ROS) play a vital role in occurrence exacerbation metabolic diseases, nanozyme offer unique perspective therapy through multifunctional achieving essential results treatment diseases directly scavenging excess ROS or regulating pathologically related molecules. The rational design strategies, nanozyme-enabled therapeutic mechanisms at cellular level, therapies several typical underlying are discussed, mainly including obesity, diabetes, cardiovascular disease, diabetic wound healing, others. Finally, pharmacokinetics, safety analysis, challenges, outlooks application also presented. This review will provide some instructive perspectives promote development strategies disease therapy. Graphical

Language: Английский

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Silica-induced macrophage pyroptosis propels pulmonary fibrosis through coordinated activation of relaxin and osteoclast differentiation signaling to reprogram fibroblasts

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 273, P. 116106 - 116106

Published: Feb. 19, 2024

Silica nanoparticle (SiNP) exposure induces severe pulmonary inflammation and fibrosis, but the pathogenesis remains unclear, effective therapies are currently lacking. To explore mechanism underlying SiNPs-induced we constructed in vivo silica animal models vitro of silica-induced macrophage pyroptosis fibroblast transdifferentiation. We found that SiNP elicits upregulation proteins associated with pyroptosis, including NLRP3, ASC, IL-1β, GSDMD, while immunofluorescence staining co-localized NLRP3 GSDMD macrophage-specific biomarker F4/80 silica-exposed lung tissues. However, inhibitor MCC950 classical anti-fibrosis drug pirfenidone (PFD) were to be able alleviate collagen deposition lungs. In studies, exposed a conditioned medium from pyroptotic macrophages enhanced expression α-SMA, suggesting increased transdifferentiation myofibroblast. line combined treatment PFD was demonstrated inhibit α-SMA attenuate Mechanistically, adopted high throughput RNA sequencing on different treatments activated signaling relaxin osteoclast differentiation pathways, where dysregulated genes these two pathways examined consistently altered both vivo. Collectively, our study demonstrates which subsequently causes myofibroblasts, play crucial roles. These findings may provide valuable references for developing new fibrosis.

Language: Английский

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Ferroptosis contributing to cardiomyocyte injury induced by silica nanoparticles via miR-125b-2-3p/HO-1 signaling

Particle and Fibre Toxicology, Journal Year: 2024, Volume and Issue: 21(1)

Published: April 1, 2024

Abstract Background Amorphous silica nanoparticles (SiNPs) have been gradually proven to threaten cardiac health, but pathogenesis has not fully elucidated. Ferroptosis is a newly defined form of programmed cell death that implicated in myocardial diseases. Nevertheless, its role the adverse effects SiNPs described. Results We first reported induction cardiomyocyte ferroptosis by both vivo and vitro . The sub-chronic exposure through intratracheal instillation aroused injury, characterized significant inflammatory infiltration collagen hyperplasia, accompanied elevated CK-MB cTnT activities serum. Meanwhile, activation was certified extensive iron overload, declined FTH1 FTL, lipid peroxidation. correlation analysis among detected indexes hinted responsible for SiNPs-aroused injury. Further, tests, triggered overload peroxidation cardiomyocytes. Concomitantly, altered expressions TfR, DMT1, FTH1, FTL indicated dysregulated metabolism cardiomyocytes upon SiNP stimuli. Also, shrinking mitochondria with ridge fracture ruptured outer membrane were noticed. To note, inhibitor Ferrostatin-1 could effectively alleviate SiNPs-induced peroxidation, cytotoxicity. More importantly, mechanistic investigations revealed miR-125b-2-3p-targeted HO-1 as key player SiNPs, probably regulating intracellular mediate ensuing Conclusions Our findings firstly underscored fact mediated miR-125b-2-3p/HO-1 signaling contributor which be importance elucidate toxicity provide new insights into future safety applications SiNPs-related nano products. Graphical

Language: Английский

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In Vitro Exposure of A549 and J774A.1 Cells to SiO₂ and TiO₂ Nanoforms and Related Cellular- and Molecular-Level Effects: Application of Proteomics

Journal of Proteome Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

There is an emerging interest in incorporating proteomic data for environmental health risk assessments. Meanwhile, the production and use of engineered nanomaterials (ENMs) with attractive physicochemical properties are expanding potential exposure, thus necessitating toxicity information on these materials analysis, where can be informative. Here, cells (A549 human lung epithelial J774A.1 mouse monocyte/macrophage cells) were exposed to ENMs (nanoforms SiO2and TiO2) different sizes surface chemistries (dose: 0-100 μg/cm2, 24 h) vitro data. Cytotoxicity (CTB, ATP, LDH), oxidative stress (GSH oxidation), analysis (MS- antibody-based) conducted post-nanoparticle (NP) exposure determine relative potency identify perturbed cellular pathways. Dose-, nanoform-, cell type-specific cytotoxicity changes observed upon both nanoSiO2 nanoTiO2. Size, agglomeration, modification, metal impurities appeared determinants cytotoxicity. Proteomic identified some enriched mechanistic pathways biological processes relevant defense/phagocytosis, stress, metabolism, apoptosis, inflammatory NPs. A549 exhibited pathway/biological transport/endocytosis, post-NP exposures. Concordance was between nanoform exposure- type-related responses, notably which critical viability, pathways/biological processes. These findings demonstrate application proteomics regulatory toxicology warrant further research this direction.

Language: Английский

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