Advances in methods and concepts provide new insight into antibiotic fluxes across the bacterial membrane DOI Creative Commons
Julia Vergalli, Matthieu Réfrégiers, Paolo Ruggerone

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Nov. 14, 2024

The sophisticated envelope of Gram-negative bacteria modulates the uptake small molecules in a side-chain-sensitive manner. Despite intensive theoretical and experimental investigations, general set pathways underpinning antibiotic has not been identified. This manuscript discusses passive influx versus active efflux antibiotics, considering responsible membrane proteins transported molecules. Recent methods have analyzed drug transport across bacterial order to understand their activity. combination vitro, cellulo silico shed light on key, mainly electrostatic, interactions between molecule surface, porins transporters during permeation. A key factor is relationship dose an compound near its target antibacterial activity critical early window. Today, methodology breakthroughs provide fruitful tools precisely dissect transport, identify steps resistance associated with impermeability efflux, highlight parameters generate more effective drugs. controls accumulation via mechanisms. article recent cellulo, highlighting "drug-transporters" dialogues proposes new perspectives overcome resistance.

Language: Английский

Flourishing Antibacterial Strategies for Osteomyelitis Therapy DOI Creative Commons
Xukai Wang, Mingran Zhang, Tongtong Zhu

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(11)

Published: Jan. 30, 2023

Abstract Osteomyelitis is a destructive disease of bone tissue caused by infection with pathogenic microorganisms. Because the complex and long‐term abnormal conditions, osteomyelitis one refractory diseases in orthopedics. Currently, anti‐infective therapy primary modality for addition to thorough surgical debridement. However, bacterial resistance has gradually reduced benefits traditional antibiotics, development advanced antibacterial agents received growing attention. This review introduces main targets treating osteomyelitis, including cell wall, membrane, intracellular macromolecules, energy metabolism, focuses on their mechanisms, predicts prospects clinical applications.

Language: Английский

Citations

61
Per/polyfluoroalkyl substances modulate plasmid transfer of antibiotic resistance genes: A balance between oxidative stress and energy support DOI
C. Liu, Xiangyu Zhu,

Luhua You

et al.

Water Research, Journal Year: 2023, Volume and Issue: 240, P. 120086 - 120086

Published: May 18, 2023

Language: Английский

Citations

44
Fabrication of Bilayer Nanofibrous-Hydrogel Scaffold from Bacterial Cellulose, PVA, and Gelatin as Advanced Dressing for Wound Healing and Soft Tissue Engineering DOI Creative Commons
Rawaiz Khan, Muhammad Umar Aslam Khan, Goran Stojanović

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(6), P. 6527 - 6536

Published: Feb. 1, 2024

Tissue engineering is currently one of the fastest-growing areas engineering, requiring fabrication advanced and multifunctional materials that can be used as scaffolds or dressings for tissue regeneration. In this work, we report a bilayer material prepared by electrospinning hybrid poly(vinyl alcohol) (PVA) bacterial cellulose (BC NFs) (top layer) over highly interconnected porous 3D gelatin-PVA hydrogel obtained freeze-drying process (bottom layer). The techniques were combined to produce an with synergistic effects on physical biological properties two materials. was characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water contact measurement system (WCMS). Studies swelling, degradability, porosity, drug release, cellular antibacterial activities performed standardized procedures assays. FTIR confirmed cross-linking both top bottom layers, SEM showed structure layer, random deposition NFs surface, aligned in cross section. angle (WCA) hydrophilic surface material. Swelling analysis high degradation good stability. released Ag-sulfadiazine sustained controlled manner against severe disease-causing gram + ive −ive (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa) strains. vitro studies fibroblasts (3T3) human embryonic kidneys (HEK-293), which desirable cell viability, proliferation, adhesion bilayer. Thus, effect resulted potential wound dressing healing soft engineering.

Language: Английский

Citations

35
Antimicrobial Peptide Coating of TiO2 Nanoparticles for Boosted Antimicrobial Effects DOI Creative Commons
Lucrezia Caselli, Tanja Traini, Samantha Micciulla

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 20, 2024

Abstract This study explores the coating of photocatalytic nanoparticles with antimicrobial peptides (AMPs) for boosted effects, and how such effects depend on AMP properties. For this, TiO 2 are coated KYE21 or its hydrophobically enhanced variant WWWKYE21. Mirroring free peptides, displayed higher binding UV‐induced degradation bacteria‐like than mammalian‐like membranes. In addition, they degraded bacterial lipopolysaccharides (LPS). WWWKYE21‐coated to LPS membranes degradation, although saturation found at high nanoparticle binding. Neutron reflectometry showed that peptide‐coated resulted in partial lipid removal absence UV, but UV illumination caused additional featuring increases hydration headgroup acyl chain regions. LPS, induced outer O‐antigen region. Analogous findings model systems, against Escherichia coli bacteria pronounced, while toxicity human monocytes remained low. Altogether, results show boosts without causing cell toxicity. From a broader perspective, points potential nanoarchitectonic combination component properties design composite NP

Language: Английский

Citations

14
Depth-Resolved Temperature-Dependent Penetration of Polymyxin B in Phospholipids/Lipopolysaccharide Asymmetric Bilayers DOI Creative Commons
Nicolò Paracini, Jeremy H. Lakey, Luke A. Clifton

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

The lipid matrix of the outer membrane (OM) Gram-negative bacteria consists a highly asymmetric bilayer containing phospholipids on inner leaflet and lipopolysaccharides (LPS) in layer. latter ensures that harmful molecules do not permeate bacterial cell, but polymyxin B (PmB), last-resort antibiotic, is capable interfering with stability LPS layer overcoming OM barrier. We have previously shown efficacy PmB disrupting isotopically models (2H-phospholipids 1H-LPS) regulated by gel-to-fluid phase transition Here, we employ fully deuterated 2H-LPS) to track temperature-dependent penetration within model using neutron reflectometry. use model-independent approach quantify as function both concentration temperature well model-dependent analysis localize bilayer. By leveraging ability neutrons differentiate hydrogen from deuterium structural biology find hijacks accumulates predominantly hydrophobic region A.

Language: Английский

Citations

1
The interaction of human serum components with model membranes containing phospholipids and lipopolysaccharides DOI Creative Commons
Nicolò Paracini,

Yubexi Correa,

Rita Del Giudice

et al.

Journal of Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 688, P. 150 - 160

Published: Feb. 20, 2025

Language: Английский

Citations

1
Biogenic Zinc Oxide Nanoparticles: An Insight into the Advancements in Antimicrobial Resistance DOI Creative Commons
Avinash Sharma,

K. Akash,

Swati Kumari

et al.

ECS Journal of Solid State Science and Technology, Journal Year: 2024, Volume and Issue: 13(4), P. 047002 - 047002

Published: April 1, 2024

Multidrug resistance (MDR) is a significant global challenge requiring strategic solutions to address bacterial infections. Recent advancements in nanotechnology, particularly the synthesis of zinc oxide nanoparticles (ZnO NPs) using natural agents as stabilizers and reducing agents, have shown promising results combating MDR. These possess strong antimicrobial properties against different strains Gram-positive Gram-negative, making them suitable for various industries, including food, pharmaceuticals, coatings, medical devices. ZnO-NPs work by generating reactive oxygen species, releasing ions (Zn2+), disrupting cell membrane, interfering with metabolic processes genetic material, inducing oxidative stress apoptosis. However, more research needed refine techniques, control size morphology, increase antibacterial efficacy. To fully understand their potential, interactions proteins, DNA, walls must also be examined. Investigating synergistic potential biogenic ZnO NPs conventional treatments could enhance therapeutic effectiveness while minimizing risk emergence. Here we provide insight into bio extracts applications well factors affecting process characterization techniques NPs. studies on activity pathogens mechanisms action are discussed. Furthermore, highlighted.

Language: Английский

Citations

8
Prevention and potential remedies for antibiotic resistance: current research and future prospects DOI Creative Commons

Rabiya Tabbassum Khan,

Vanshika Sharma,

Sofia Sharief Khan

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 3, 2024

The increasing threat of antibiotic resistance and shrinking treatment options for infections have pushed mankind into a difficult position. looming the return pre-antibiotic era has caused sense urgency to protect conserve potency therapy. One perverse effects is dissemination its causative agents from non-clinically important strains clinically vice versa. popular saying "Prevention better than cure" appropriate tackling resistance. On one hand, new effective antibiotics are required; on other measures use antibiotics, along with increased awareness in general public related use, essential. Awareness, especially resistance, dissemination, potential threats, can help greatly controlling abuse containment Antibiotic drugs' effectiveness be enhanced by producing novel analogs or adding adjuvants current antibiotics. Combinatorial therapy proven successful treating multidrug-resistant (MDR) bacterial infections. This review aims highlight global situation discuss methods used monitor, prevent, inhibit, reverse mechanisms fight against

Language: Английский

Citations

8
Diversity, Complexity, and Specificity of Bacterial Lipopolysaccharide (LPS) Structures Impacting Their Detection and Quantification DOI Open Access
Flavien Dardelle, Capucine Phelip, Maryam Darabi

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3927 - 3927

Published: March 31, 2024

Endotoxins are toxic lipopolysaccharides (LPSs), extending from the outer membrane of Gram-negative bacteria and notorious for their toxicity deleterious effects. The comparison different LPSs, isolated various bacteria, shows a global similar architecture corresponding to glycolipid lipid A moiety, core oligosaccharide, outermost long O-chain polysaccharides with molecular weights 2 20 kDa. LPSs display high diversity specificity among genera species, each bacterium contains unique set LPS structures, constituting its protective external barrier. Some not due particular structures. Different, well-characterized, highly purified were used in this work determine endotoxin detection rules identify impact on host. Endotoxin is major task ensure safety human health, especially pharma food sectors. Here, we describe structures obtained under bacterial growth conditions selective methods such as LAL, HEK-blue TLR-4, LC-MS2, MALDI-MS. In these assays, shown respond differently, mainly attributable fatty acid numbers chain lengths, presence phosphate groups, possible substitutions.

Language: Английский

Citations

5
Faster but Not Sweeter: A Model of Escherichia coli Re-level Lipopolysaccharide for Martini 3 and a Martini 2 Version with Accelerated Kinetics DOI Creative Commons
Astrid F. Brandner, Dheeraj Prakaash, Alexandre Blanco-González

et al.

Journal of Chemical Theory and Computation, Journal Year: 2024, Volume and Issue: 20(15), P. 6890 - 6903

Published: July 15, 2024

Lipopolysaccharide (LPS) is a complex glycolipid molecule that the main lipidic component of outer leaflet membrane Gram-negative bacteria. It has very limited lateral motion compared to phospholipids, which are more ubiquitous in biological membranes, including inner The slow-moving nature LPS can present hurdle for molecular dynamics simulations, given (pragmatically) accessible timescales simulations currently microseconds, during displays some conformational but hardly any diffusion. Thus, it not feasible observe phenomena such as insertion molecules, antibiotics/antimicrobials, directly into from extracellular side nor dissociating proteins via using available models at atomistic and coarse-grained levels granularity. Here, we model deep rough compatible with Martini 2 force field scaled down nonbonded interactions enable faster We show faster-diffusing able reproduce salient biophysical properties standard models, due its motion, molecules penetrate deeper membranes containing model. fast ReLPS experimentally determined patterns interaction while also allowing associate dissociate within microsecond timescales. complete 3 toolkit Escherichia coli by presenting (standard) this field.

Language: Английский

Citations

5