Fabrication of Bilayer Nanofibrous-Hydrogel Scaffold from Bacterial Cellulose, PVA, and Gelatin as Advanced Dressing for Wound Healing and Soft Tissue Engineering

ACS Omega, Journal Year: 2024, Volume and Issue: 9(6), P. 6527 - 6536

Published: Feb. 1, 2024

Tissue engineering is currently one of the fastest-growing areas engineering, requiring fabrication advanced and multifunctional materials that can be used as scaffolds or dressings for tissue regeneration. In this work, we report a bilayer material prepared by electrospinning hybrid poly(vinyl alcohol) (PVA) bacterial cellulose (BC NFs) (top layer) over highly interconnected porous 3D gelatin-PVA hydrogel obtained freeze-drying process (bottom layer). The techniques were combined to produce an with synergistic effects on physical biological properties two materials. was characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water contact measurement system (WCMS). Studies swelling, degradability, porosity, drug release, cellular antibacterial activities performed standardized procedures assays. FTIR confirmed cross-linking both top bottom layers, SEM showed structure layer, random deposition NFs surface, aligned in cross section. angle (WCA) hydrophilic surface material. Swelling analysis high degradation good stability. released Ag-sulfadiazine sustained controlled manner against severe disease-causing gram + ive −ive (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa) strains. vitro studies fibroblasts (3T3) human embryonic kidneys (HEK-293), which desirable cell viability, proliferation, adhesion bilayer. Thus, effect resulted potential wound dressing healing soft engineering.

Language: Английский

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Current Perspectives of Protein in Bone Tissue Engineering: Bone Structure, Ideal Scaffolds, Fabrication Techniques, Applications, Scopes, and Future Advances

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(8), P. 5082 - 5106

Published: July 15, 2024

In view of their exceptional approach, excellent inherent biocompatibility and biodegradability properties, interaction with the local extracellular matrix, protein-based polymers have received attention in bone tissue engineering, which is a multidisciplinary field that repairs regenerates fractured bones. Bone multihierarchical complex structure, it performs several essential biofunctions, including maintaining mineral balance structural support protecting soft organs. Protein-based gained interest developing ideal scaffolds as emerging biomaterials for healing regeneration, challenging to design substitutes perfect biomaterials. Several polymers, collagen, keratin, gelatin, serum albumin, etc., are potential materials due cytocompatibility, controlled biodegradability, high biofunctionalization, tunable mechanical characteristics. While numerous studies indicated encouraging possibilities proteins BTE, there still major challenges concerning stability physiological conditions, continuous release growth factors bioactive molecules. Robust derived from can be used replace broken or diseased biocompatible substitute; proteins, being biopolymers, provide engineering. Herein, recent developments protein cutting-edge engineering addressed this review within 3–5 years, focus on significant future perspectives. The first section discusses fundamentals anatomy scaffolds, second describes fabrication techniques scaffolds. third highlights importance applications BTE. Hence, development state-of-the-art has been discussed, highlighting

Language: Английский

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Injectable hemostatic hydrogel adhesive with antioxidant, antibacterial and procoagulant properties for hemorrhage wound management

Journal of Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 673, P. 395 - 410

Published: June 6, 2024

Language: Английский

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Hydrogels: Classifications, fundamental properties, applications, and scopes in recent advances in tissue engineering and regenerative medicine – A comprehensive review

Arabian Journal of Chemistry, Journal Year: 2024, Volume and Issue: 17(10), P. 105968 - 105968

Published: Aug. 17, 2024

Hydrogels are three-dimensional structures that serve as substitutes for the extracellular matrix (ECM) and possess outstanding physicochemical biochemical characteristics. They gaining importance in regenerative medicine because of their similarity to natural terms moisture content wound tissue healing permeability. Tissue engineering advancements have resulted development flexible hydrogels mimic dynamic characteristics ECM. Several approaches been applied produce from biopolymers with enhanced functional structural different applications (TERM). This review provides a comprehensive overview hydrogel healing, engineering, drug delivery systems. We outline types based on physical chemical crosslinking, fundamental properties, TERM. article provided recent literature within five years. Recent developments biopolymer-based state-of-the-art discussed, emphasizing significant challenges future perspectives.

Language: Английский

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Unlocking the biological response of smart Stimuli-Responsive hydrogels and their application in biological systems

European Polymer Journal, Journal Year: 2024, Volume and Issue: 209, P. 112906 - 112906

Published: March 6, 2024

Language: Английский

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Cartilage tissue engineering using decellularized biomatrix hydrogel containing TGF-β-loaded alginate microspheres in mechanically loaded bioreactor

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 25, 2024

Abstract Physiochemical tissue inducers and mechanical stimulation are both efficient variables in cartilage fabrication regeneration. In the presence of biomolecules, decellularized extracellular matrix (ECM) may trigger enhance stem cell proliferation differentiation. Here, we investigated controlled release transforming growth factor beta (TGF-β1) as an active mediator mesenchymal stromal cells (MSCs) a biocompatible scaffold for engineering. ECM-derived hydrogel with TGF-β1-loaded alginate-based microspheres (MSs) was created to promote human MSC chondrogenic development. Ex vivo explants complicated multiaxial loading bioreactor replicated physiological conditions. Hydrogels with/without MSs TGF-β1 were highly cytocompatible. MSCs containing TGF-β1/MSs showed comparable gene expression levels those hydrogels added culture media or without TGF-β1. However, constructs directly within had inferior properties under unloaded The group including circumstances formed better ex osteochondral defect than control settings. This study demonstrates that local delivery using is essential neocartilage formation by further optimization needed prevent differentiation towards hypertrophy.

Language: Английский

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Natural blackcurrant extract contained gelatin hydrogel with photothermal and antioxidant properties for infected burn wound healing

Materials Today Bio, Journal Year: 2024, Volume and Issue: 26, P. 101113 - 101113

Published: June 1, 2024

Burns represent a prevalent global health concern and are particularly susceptible to bacterial infections. Severe infections may lead serious complications, posing life-threatening risk. Near-infrared (NIR)-assisted photothermal antibacterial combined with antioxidant hydrogel has shown significant potential in the healing of infected wounds. However, existing agents typically metal-based, complicated synthesize, or pose biosafety hazards. In this study, we utilized plant-derived blackcurrant extract (B) as natural source for both properties. By incorporating B into G-O crosslinked through Schiff base reaction between gelatin (G) oxidized pullulan (O), resulting G-

Language: Английский

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Transparent and Stretchable Conductive Hydrogel Sensors: Optimizing Ion Selection to Enhance Mechanical and Sensing Performance

ACS Applied Electronic Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Conductive hydrogels, with their unique combination of electrical conductivity and stretchability, are emerging as critical components for next-generation, flexible, wearable sensors. In this work, we aimed to develop a low-cost, easy-to-manufacture hydrogel sensor using ionic compounds the source conductivity. Hydrogels were synthesized acrylamide, poly(ethylene glycol), carboxymethyl cellulose, systematic variation LiCl, NaCl, KCl, labeled Li-CH, Na-CH, K-CH, respectively, explore effects on nanostructure mechanical properties hydrogels. Among different formulations, Na-CH demonstrated superior performance optimized elongation at break, tensile strength, toughness, highlighting importance ion selection in design. also exhibited excellent outstanding transparency, high sensitivity detecting wide range body movements, from large-scale gestures subtle physiological signals such pulse detection. With its exceptional robustness, repeatable sensing performance, shows great potential future applications flexible electronics, healthcare monitoring systems, smart display technologies.

Language: Английский

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Engineered hydrogel platform for diabetic wound healing

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160379 - 160379

Published: Feb. 1, 2025

Language: Английский

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Polydopamine reduced graphene oxide/ chitosan-based hydrogel for the therapy of diabetic wound

Materials Today Communications, Journal Year: 2024, Volume and Issue: 39, P. 109319 - 109319

Published: May 27, 2024

Language: Английский

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