S-adenosyl-L-methionine alleviates the senescence of ADSCs through the PI3K/AKT/FOXO3a signaling pathway DOI Creative Commons

Lipeng Shang,

Xiaoxia Li, Xiaoyan Ding

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: July 28, 2023

Abstract BackgroundThe senescence of mesenchymal stem cells (MSCs) greatly compromises their therapeutic effect in regenerative medicine. Numerous studies are focusing on possible rejuvenation strategies to enhance the efficacy autologous MSC-based therapy. S-adenosyl-L-methionine (SAM) is a metabolite present all living cells. However, there little research about effects and mechanisms SAM MSCs.MethodsIn this study, adipose-derived MSC (ADSC) were assessed vitro by β-galactosidase staining, reactive oxygen species assay, cell cycle tests, Western blot. The osteogenic adipogenic differentiation ability changes also detected. Mouse models premature aging established subcutaneous injection D-Galactose (D-gal). vivo antiaging roles tested through behavioral organ coefficient, pathological morphology, expression aging-related proteins major organs, such as heart, liver, kidney. bone density distal femur, well volume, number, thickness trabecula evaluated micro-CT. molecular searched validated transcriptome sequencing, blot, immunofluorescence. Small RNA interfering was used knock down FOXO3a.ResultsIn oxidative stress–induced senescent ADSCs, ameliorated arrest, reduced activity, inhibited P53 P21, restored SIRT1, which significantly improved biological function. In addition, level ROS promoted ADSCs. Moreover, D-gal-induced mouse model aging, exercise kidney mice. Additionally, number trabeculae femur mice increased. Transcriptome sequencing results revealed that PI3K/AKT involved SAM-mediated anti-senescence MSCs. Mechanistically, activated signaling increased phosphorylation FOXO3a, resulting decrease translocation FOXO3a nucleus inhibition activity P21. Ultimately, attenuated ADSCs potential.ConclusionsIn summary, our suggested could ameliorate MSCs PI3K/AKT/FOXO3a signaling. Meanwhile, exert vivo.

Language: Английский

20(S)‐protopanaxatriol inhibited D‐galactose‐induced brain aging in mice via promoting mitochondrial autophagy flow DOI

Jun‐jie Zhang,

Rui‐yi Hu,

Ke‐cheng Chen

et al.

Phytotherapy Research, Journal Year: 2023, Volume and Issue: 37(7), P. 2827 - 2840

Published: April 10, 2023

Previous reports have confirmed that saponins (ginsenosides) derived from Panax ginseng. C. A. Meyer exerted obvious memory-enhancing and antiaging effects, the simpler structure of ginsenosides, better biological activity. In this work, we aimed to explore therapeutic effect underlying molecular mechanism 20(S)-protopanaxatriol (PPT), aglycone panaxatriol-type by establishing D-galactose (D-gal)-induced subacute brain aging model in mice. The results showed PPT treatment (10 20 mg/kg) for 4 weeks could significantly restore D-gal (800 mg/kg 8 weeks)-induced impaired memory function, choline dysfunction, redox system imbalance Meanwhile, also reduced histopathological changes caused exposure. Moreover, increase TFEB/LAMP2 protein expression promote mitochondrial autophagic flow. Importantly, docking had good binding ability with LAMP2 TFEB, suggesting might play an important role alleviate D-gal-caused aging.

Language: Английский

Citations

8
The natural source, physicochemical properties, biological activities and metabolism of astilbin DOI

Dan Yang,

Qing‐Feng Zhang

Critical Reviews in Food Science and Nutrition, Journal Year: 2022, Volume and Issue: 63(28), P. 9506 - 9518

Published: April 20, 2022

Astilbin is a dihydroflavanol found in many plants and processed foods. possesses multiple health-beneficial bioactivities has received great attention. Hence, the natural source, physicochemical properties, biological activities metabolism of astilbin are summarized present article. Engelhardia roxburghiana Wall. Smilax glabra Roxb. main resource for purification because high content. Because chemical instability, amount foods dependent on processing storage conditions. The degradation includes isomerization decomposition. interconversion its isomers occurs through chalcone intermediates, which significantly affects taste wine during storage. Many factors such as temperature, pH, metal ions food additives could affect stability astilbin. exhibits very novel selective immunosuppressive activity, not other compounds. rhamnose moiety essential this bioactivity. After digestion, was mainly absorbed transported circulatory blood intact form, only one metabolite, 3'-O-methylastilbin, found. Although having bioactivities, faces challenge poor bioavailability. Some promising strategies were developed improving bioavailability, particularly fabrication zein nanoparticles.

Language: Английский

Citations

13
Genome-wide scans for selection signatures in indigenous pigs revealed candidate genes relating to heat tolerance DOI Creative Commons
Ziqi Zhong, Rui Li, Zonghua Wang

et al.

animal, Journal Year: 2023, Volume and Issue: 17(7), P. 100882 - 100882

Published: June 16, 2023

Heat stress is a major problem that constrains pig productivity. Understanding and identifying adaptation to heat has been the focus of recent studies, identification genome-wide selection signatures can provide insights into mechanisms environmental adaptation. Here, we generated whole genome re-sequencing data from six Chinese indigenous populations identify genomic regions with related tolerance using multiple methods: three methods for intra-population analyses (Integrated Haplotype Score, Runs Homozygosity Nucleotide diversity Analysis) (iHS, ROH, Pi) inter-population (Fixation index, Cross-population Composite Likelihood Ratio Extended Homozygosity) (FST, XP-CLR, XPEHH). In total, 1 966 796 single nucleotide polymorphisms (SNPs) were identified in this study. Genetic structure FST indicated differentiation among these breeds. Based on information location environment, breeds divided cold groups. By combining two or more approaches signatures, outlier signals overlapping as candidate regions. A total 163 genes identified, which, 29 associated injury anti-inflammatory effects. These further 78 Gene Ontology (GO) functional terms 30 Kyoto Encyclopedia Genes Genomes (KEGG) pathways enrichment analysis (P < 0.05). Some have clear relevance resistance, such AMPK signaling pathway mTOR pathway. The results improve our understanding responsible resistance pigs new introgression

Language: Английский

Citations

8
Molecular Insight into the Binding of Astilbin with Human Serum Albumin and Its Effect on Antioxidant Characteristics of Astilbin DOI Creative Commons
Xiangyu Han, Jing Sun,

Tianmei Niu

et al.

Molecules, Journal Year: 2022, Volume and Issue: 27(14), P. 4487 - 4487

Published: July 13, 2022

Astilbin is a dihydroflavonol glycoside identified in many natural plants and functional food with promising biological activities which used as an antioxidant the pharmaceutical fields. This work investigated interaction between astilbin human serum albumin (HSA) their effects on activity of by multi-spectroscopic molecular modeling studies. The experimental results show that quenches fluorescence emission HSA through static quenching mechanism. prefer to bind at Site Ⅰ position, mainly maintained electrostatic force, hydrophobic hydrogen bonding interactions. Multi-spectroscopic MD indicate secondary structure could be changed because HSA. DPPH radical scavenging assay shows presence reduces capacity astilbin. explication astilbin-HSA binding mechanism will provide insights into clinical use resource development industries.

Language: Английский

Citations

12
Downregulation of Sirt6 by CD38 promotes cell senescence and aging DOI Creative Commons
Hongji Zhou, Shihai Liu,

NanYang Zhang

et al.

Aging, Journal Year: 2022, Volume and Issue: unknown

Published: Dec. 6, 2022

Decreased nicotinamide adenine dinucleotide (NAD+) levels accompany aging. CD38 is the main cellular NADase. Cyanidin-3-O-glucoside (C3G), a natural inhibitor of CD38, well-known drug that extends human lifespan. We investigated mechanisms in cell senescence and C3G antiaging. Myocardial H9c2 cells were induced to with D-gal. siRNA, UBCS039 (a chemical activator Sirt6) inhibited D-gal-induced by reducing reactive oxygen species, hexokinase 2 SA-β-galactosidase levels. These activators also stimulated proliferation telomerase reverse transcriptase levels, while OSS-128167 Sirt6 siRNA exacerbated senescent process. underwent increased expression decreased expression; expression, respectively; presence siRNA. In acute aging mice, exhibited respectively, myocardial tissues, treatment tissues. reduced IL-1β, IL-6, IL-17A, TNF-α restored NAD+ NK animals. suggest downregulates promote exerts an antiaging effect through CD38-Sirt6 signaling.

Language: Английский

Citations

12
Folic acid alleviated oxidative stress-induced telomere attrition and inhibited apoptosis of neurocytes in old rats DOI
Dezheng Zhou, Yue Sun,

Cuixia Dong

et al.

European Journal of Nutrition, Journal Year: 2023, Volume and Issue: 63(1), P. 291 - 302

Published: Oct. 23, 2023

Language: Английский

Citations

5
Therapeutic effect and underlying mechanism of Shenkang injection against cisplatin–induced acute kidney injury in mice DOI

Jiahan Su,

Tingting He,

Jing You

et al.

Journal of Ethnopharmacology, Journal Year: 2022, Volume and Issue: 301, P. 115805 - 115805

Published: Oct. 7, 2022

Language: Английский

Citations

9
Radioprotective efficacy of Astilbin in mitigating radiation‐induced lung injury through inhibition of p53 acetylation DOI

Lixing Liang,

Yaqin Huang, Liuyin Chen

et al.

Environmental Toxicology, Journal Year: 2023, Volume and Issue: 38(12), P. 2967 - 2980

Published: Aug. 20, 2023

Radiation-induced lung injury (RILI) is a common side effect in thoracic tumor patients undergoing radiotherapy. At present, there no ideal radio-protective agent which widely used RILI treatment. Astilbin (AST), bioactive flavonoid, exhibits various biological effects, including anti-inflammatory, antioxidant, and anti-fibrotic activities, partly result from reducing oxidative stress inflammation pathogenic conditions. However, the protective efficacy of AST to ameliorate has not been reported. In this study, we employed network pharmacology, RNA sequencing, experimental evaluation reveal effects pharmacological mechanism treat vivo vitro. We observed that reduced radiation-induced apoptosis, DNA damage, inflammatory reactions, reactive oxygen species (ROS) level human normal epithelial cells BEAS-2B. Further study showed treatment significantly ameliorated by pathology changes reaction tissue C57BL/6J mice. Mechanistically, expression epithelial-mesenchymal transition (EMT) markers radiation-triggered acetylation p53 protein were alleviated Furthermore, after intervention Trichostatin A (TSA). Our data indicate can alleviate inhibiting reactions EMT process through decreasing acetylation. conclusion, our suggests great potential be new therapeutic compound for RILI.

Language: Английский

Citations

4
Phillyrin Ameliorates Oxidative Stress in D-Galactose-Induced Senescence in the Brain of Mice by Regulating the Nrf2/ HO-1 Signaling Pathway DOI Creative Commons
Xuemin Li, Sheng Wang, Chenyang Li

et al.

Natural Product Communications, Journal Year: 2023, Volume and Issue: 18(5)

Published: May 1, 2023

Objective To investigate the effects of phillyrin on D-galactose (D-gal)-induced aging in brain mice and mechanism. Methods Institute Cancer Research were intraperitoneally injected with D-gal 150 mg/kg to induce model, treated 5, 15, 45 once a day for 8 weeks. Results The memory impairment induced by was ameliorated treatment, oxidative stress serum inhibited, as evidenced increased superoxide dismutase, glutathione peroxidase enzyme activities, decreased malondialdehyde levels. Additionally, hippocampus damage has also been well improved phillyrin. Moreover, protein expressions nuclear factor erythroid-2-related 2 (Nrf2), heme oxygenase-1 (HO-1), NADPH quinone oxidoreductase 1 (NQO1) markedly up-regulated expression Keap-1 down-regulated group than that model group. Conclusion These findings suggest is effective prevention age-related impairment, attenuation activating Nrf2/HO-1 signaling pathway may be one mechanisms.

Language: Английский

Citations

3
Exploring the Protective Effect and Mechanism of Buddlejae Flos on Sodium Selenite-Induced Cataract in Rats by Network Pharmacology, Molecular Docking, and Experimental Validation DOI Open Access

Xian-Yin Liu,

Xuelin Wang,

Mo-Chang Qiu

et al.

Evidence-based Complementary and Alternative Medicine, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 18

Published: May 14, 2022

Buddlejae Flos has a long history of utilization by humans to treat ophthalmic diseases. Although in vitro study revealed that it can be used for treating cataract, the bioactive components and mechanism efficacy remained unclear. This aims discover mode cataract treatment.Several databases were screened corresponding targets, as well cataract-related targets. Using String database, common targets determined utilized construct protein-protein interactions (PPI). The drug-component-target-disease network map was drawn using Cytoscape software. R language execute Kyoto Encyclopedia Genes Genomes (KEGG) Gene Ontology (GO) pathway enrichment analysis. Molecular docking done through Schrödinger Maestro software utilization. Luteolin's (LUT) effect on induced sodium selenite rat pups evaluated.Six with 38 identified being associated cataract. TP53, AKT1, EGFR, CASP3, TNF, ESR1, INS, IL6, HIF1A, VEGFA core PPI analysis, binding energy LUT AKT lowest. been demonstrated significantly lower MDA levels, raise glutathione (GSH) boost activity antioxidant enzymes like GST, SOD, GPx, CAT. After treatment, TNF-a, IL-2, IL-6 levels lowered. Bcl-2 mRNA expression p-PI3K p-AKT protein elevated. In contrast, caspase-3 Bax decreased.This demonstrates is possible component may treatment. Its action includes oxidative stress suppression, reducing inflammation, inhibiting apoptosis via regulating PI3K/AKT single pathway.

Language: Английский

Citations

5