A Library of ROS‐Catalytic Metalloenzyme Mimics with Atomic Metal Centers DOI
Sujiao Cao,

Zhenyang Zhao,

Yijuan Zheng

et al.

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(16)

Published: Feb. 8, 2022

MetalN-coordinated centers supported by carbonaceous substrates have emerged as promising artificial metalloenzymes (AMEs) to mimic the biocatalytic effects of their natural counterparts. However, synthesis well-defined AMEs that contain different atomic metalN but present similar physicochemical and coordination structures remains a substantial challenge. Here, 20 types with geometries metalN-coordinated are synthesized compare disclose catalytic activities, substrate selectivities, kinetics, reactive oxygen species (ROS) products. Their oxidase (OXD)-, peroxidase (POD)-, halogen (HPO)-mimetic behaviors systematically explored. The Fe-AME shows highest OXD- HPO-mimetic activities compared other due its high vmax (0.927 × 10-6 m s-1 ) low Km (1.070 10-3 m), while Cu-AME displays best POD-like performance. Furthermore, theoretical calculation reveals ROS-catalytic paths highly related electronic metal centers. Benefiting from facile adsorption H2 O2 molecule lower energy barrier generating •O2- , higher performances than Mn-AME. engineered show not only remarkably also provide new guidance toward developing biocatalysts for broad application fields.

Language: Английский

Nanozymes Inspired by Natural Enzymes DOI Open Access
Ruofei Zhang, Xiyun Yan, Kelong Fan

et al.

Accounts of Materials Research, Journal Year: 2021, Volume and Issue: 2(7), P. 534 - 547

Published: June 24, 2021

ConspectusNanozymes, nanomaterials with enzyme-like activities high structural stability, adjustable catalytic activity, functional diversity, recyclability, and feasibility in large-scale preparation, have become a hot spot the field of artificial enzymes recent years are expected to potential surrogates competitors for natural practical applications. With development in-depth research wide range application requirements, creating nanozymes performance comparable or even surpassing that has been key topic this field. Most reported past were obtained based on random synthesis screening, which efficiency is far inferior enzymes. Natural evolved over hundreds millions developed lot high-efficiency catalysis know-how hidden their features. To create highly active nanozymes, we assumed there general structure–activity relationship between proposed nanozyme optimization strategy by grafting principles into rational design nanozymes. On basis bioinspired strategy, series exhibit similar closer beyond those successfully synthesized. By now, rationally designed high-activity current nanozymes.In Account, focus representative progress systemic construction devoted introducing strategic concepts We show de novo simulating amino acid microenvironment using metal-free architecture coordination structure metal sites an effective significantly improving A future perspective challenges countermeasures these achievements. hope biologically inspired perception will arouse widespread interest fundamental applications as well provide inspiration

Language: Английский

Citations

481
Nanozymes: A clear definition with fuzzy edges DOI
Hui Wei, Lizeng Gao, Kelong Fan

et al.

Nano Today, Journal Year: 2021, Volume and Issue: 40, P. 101269 - 101269

Published: Aug. 20, 2021

Language: Английский

Citations

464
A Review on Metal- and Metal Oxide-Based Nanozymes: Properties, Mechanisms, and Applications DOI Creative Commons
Yongyu Liu, Amin Zhang, Ruhao Wang

et al.

Nano-Micro Letters, Journal Year: 2021, Volume and Issue: 13(1)

Published: July 9, 2021

Since the ferromagnetic (Fe3O4) nanoparticles were firstly reported to exert enzyme-like activity in 2007, extensive research progress nanozymes has been made with deep investigation of diverse and rapid development related nanotechnologies. As promising alternatives for natural enzymes, have broadened way toward clinical medicine, food safety, environmental monitoring, chemical production. The past decade witnessed metal- metal oxide-based owing their remarkable physicochemical properties parallel low cost, high stability, easy storage. It is widely known that study catalytic activities mechanism sheds significant influence on applications nanozymes. This review digs into characteristics intrinsic nanozymes, especially emphasizing recent biological analysis, relieving inflammation, antibacterial, cancer therapy. We also conclude present challenges provide insights future constituted oxide nanomaterials.

Language: Английский

Citations

435
Nanozyme for tumor therapy: Surface modification matters DOI Creative Commons

Guoheng Tang,

Jiuyang He,

Juewen Liu

et al.

Exploration, Journal Year: 2021, Volume and Issue: 1(1), P. 75 - 89

Published: Aug. 1, 2021

As the next generation of artificial enzymes, nanozymes have shown unique properties compared to its natural counterparts, such as stability in harsh environment, low cost, and ease production modification, paving way for biomedical applications. Among them, tumor catalytic therapy mediated by reactive oxygen species (ROS) has made great progress mainly from peroxidase-like activity nanozymes. Fe

Language: Английский

Citations

342
Deciphering the catalytic mechanism of superoxide dismutase activity of carbon dot nanozyme DOI Creative Commons
Wenhui Gao,

Jiuyang He,

Lei Chen

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Jan. 11, 2023

Nanozymes with superoxide dismutase (SOD)-like activity have attracted increasing interest due to their ability scavenge anion, the origin of most reactive oxygen species in vivo. However, SOD nanozymes reported thus far yet approach natural enzymes. Here, we report a carbon dot (C-dot) nanozyme catalytic over 10,000 U/mg, comparable that Through selected chemical modifications and theoretical calculations, show SOD-like C-dots relies on hydroxyl carboxyl groups for binding anions carbonyl conjugated π-system electron transfer. Moreover, C-dot exhibit intrinsic targeting oxidation-damaged cells effectively protect neuron ischemic stroke male mice model. Together, our study sheds light structure-activity relationship nanozymes, demonstrates potential treating oxidation stress related diseases.

Language: Английский

Citations

315
Nanozymes: Definition, Activity, and Mechanisms DOI Creative Commons
Mohamad Zandieh, Juewen Liu

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(10)

Published: Feb. 17, 2023

Abstract “Nanozyme” is used to describe various catalysts from immobilized inorganic metal complexes, enzymes nanoparticles. Here, the history of nanozymes dvescribed in detail, and they can be largely separated into two types. Type 1 refer or on nanomaterials, which were dominant first decade since 2004. 2 nanozymes, rely surface catalytic properties are dominating type past decade. The definition evolving, a based same substrates products as able cover most currently claimed although may have different mechanisms compared their enzyme counterparts. A broader inspire application‐based research replace with nanomaterials for analytical, environmental, biomedical applications. Comparison also requires clear nanozyme unit. Four ways defining unit described, iron oxide horseradish peroxidase activity comparison examples each definition. Growing work devoted understanding mechanism provides basis further rational engineering active sites. Finally, future perspective field discussed.

Language: Английский

Citations

308
Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery DOI

Lishuai Feng,

Chaoran Dou,

Yuguo Xia

et al.

ACS Nano, Journal Year: 2021, Volume and Issue: 15(2), P. 2263 - 2280

Published: Jan. 11, 2021

Oxidative stress and a series of excessive inflammatory responses are major obstacles for neurological functional recovery after ischemic stroke. Effective noninvasive anti-inflammatory therapies urgently needed. However, unsatisfactory therapeutic efficacy current drugs inadequate drug delivery to the damaged brain problems. Nanozymes with robust antioxidative properties possess possibility insufficiency nanozyme accumulation in by administration hindered their application. Herein, we report neutrophil-like cell-membrane-coated mesoporous Prussian blue (MPBzyme@NCM) realize active-targeting therapy stroke improving based on innate connection between inflamed microvascular endothelial cells neutrophils The long-term vivo MPBzyme@NCM was illustrated detail being delivered into uptake microglia. Moreover, detailed mechanism via microglia further studied, including polarization toward M2, reduced recruitment neutrophils, decreased apoptosis neurons, proliferation neural stem cells, neuronal precursors, neurons. This strategy may provide an applicative perspective diseases.

Language: Английский

Citations

264
Depletable peroxidase-like activity of Fe3O4 nanozymes accompanied with separate migration of electrons and iron ions DOI Creative Commons
Haijiao Dong, Wei Du, Jian Dong

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Sept. 12, 2022

As pioneering Fe

Language: Английский

Citations

257
A Bioinspired Five‐Coordinated Single‐Atom Iron Nanozyme for Tumor Catalytic Therapy DOI
Bolong Xu, Shanshan Li, Lirong Zheng

et al.

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(15)

Published: Feb. 1, 2022

Single-atom nanozymes (SAzymes) represent a new research frontier in the biomedical fields. The rational design and controllable synthesis of SAzymes with well-defined electronic geometric structures are essential for maximizing their enzyme-like catalytic activity therapeutic efficacy but remain challenging. Here, melamine-mediated pyrolysis activation strategy is reported fabrication iron-based SAzyme containing five-coordinated structure (FeN5 ), identified by transmission electron microscopy imaging X-ray absorption fine analyses. FeN5 exhibits superior peroxidase-like owing to optimized coordination structure, corresponding efficiency Fe-species 7.64 3.45 × 105 times higher than those traditional FeN4 Fe3 O4 nanozyme, respectively, demonstrated steady-state kinetic assay. In addition, mechanism jointly disclosed experimental results density functional theory studies. as-synthesized demonstrates significantly enhanced antitumor effect vitro vivo due excellent under tumor microenvironment.

Language: Английский

Citations

248
Advances in oxidase-mimicking nanozymes: Classification, activity regulation and biomedical applications DOI
Yu Chong, Qiang Liu, Cuicui Ge

et al.

Nano Today, Journal Year: 2021, Volume and Issue: 37, P. 101076 - 101076

Published: Jan. 14, 2021

Language: Английский

Citations

245