Exercise-Intervened Endothelial Progenitor Cell Exosomes Protect N2a Cells by Improving Mitochondrial Function DOI Open Access
Shuzhen Chen, Smara Sigdel, Harshal Sawant

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1148 - 1148

Published: Jan. 17, 2024

We have recently demonstrated that exosomal communication between endothelial progenitor cells (EPCs) and brain is compromised in hypertensive conditions, which might contribute to the poor outcomes of stroke subjects with hypertension. The present study investigated whether exercise intervention can regulate EPC–exosome (EPC-EX) functions conditions. Bone marrow EPCs from sedentary exercised transgenic mice were used for generating EPC-EXs, denoted as R-EPC-EXs R-EPC-EXET. microRNA profile was analyzed, EX determined a co-culture system N2a challenged by angiotensin II (Ang II) plus hypoxia. EX-uptake efficiency, cellular survival ability, reactive oxygen species (ROS) production, mitochondrial membrane potential, expressions cytochrome c superoxide-generating enzyme (Nox4) assessed. found (1) improves uptake efficiency EPC-EXs cells. (2) restores miR-27a levels R-EPC-EXs. (3) R-EPC-EXET improved ability reduced ROS overproduction Ang (4) potential decreased Nox4 hypoxia-injured All these effects significantly inhibitor. Together, data exercise-intervened function be ascribed their carried miR-27a.

Language: Английский

Exosomes as mediators of intercellular crosstalk in metabolism DOI

Roi Isaac,

Felipe C.G. Reis, Wei Ying

et al.

Cell Metabolism, Journal Year: 2021, Volume and Issue: 33(9), P. 1744 - 1762

Published: Sept. 1, 2021

Language: Английский

Citations

466
Exosome-encapsulated miRNAs contribute to CXCL12/CXCR4-induced liver metastasis of colorectal cancer by enhancing M2 polarization of macrophages DOI
Dong Wang, Xiaohui Wang,

Mahan Si

et al.

Cancer Letters, Journal Year: 2020, Volume and Issue: 474, P. 36 - 52

Published: Jan. 10, 2020

Language: Английский

Citations

280
Exosomes as a Multicomponent Biomarker Platform in Cancer DOI
Valerie S. LeBleu, Raghu Kalluri

Trends in cancer, Journal Year: 2020, Volume and Issue: 6(9), P. 767 - 774

Published: April 16, 2020

Language: Английский

Citations

238
MicroRNAs in Pancreatic Cancer: biomarkers, prognostic, and therapeutic modulators DOI Creative Commons

Afra Z. Daoud,

Eoghan J. Mulholland, Grace Cole

et al.

BMC Cancer, Journal Year: 2019, Volume and Issue: 19(1)

Published: Nov. 21, 2019

A severe lack of early diagnosis coupled with resistance to most available therapeutic options renders pancreatic cancer as a major clinical concern. The limited efficacy current treatments necessitates the development novel strategies that are based on an understanding molecular mechanisms involved in progression. MicroRNAs (miRNAs) non-coding small RNAs regulate expression multiple proteins post-translation process and thus have promise biomarkers, prognostic agents, advanced therapies. Profiling deregulated miRNAs can correlate diagnosis, indicate optimal treatment predict response therapy. Furthermore, main effector genes along downstream pathways identify possible candidates. Additionally, obstacles translation into clinic also considered. Distinct miRNA profiles stages malignant disease, hold potential markers targets. However, validation specific role such stunts application. Target prediction using algorithms provides wide range targets, but these still require through pre-clinical studies determine knock-on genetic effects.

Language: Английский

Citations

189
The Crosstalk Between Tumor Cells and the Immune Microenvironment in Breast Cancer: Implications for Immunotherapy DOI Creative Commons
Vincenzo Salemme, Giorgia Centonze, Federica Cavallo

et al.

Frontiers in Oncology, Journal Year: 2021, Volume and Issue: 11

Published: March 11, 2021

Breast cancer progression is a complex process controlled by genetic and epigenetic factors that coordinate the crosstalk between tumor cells components of microenvironment (TME). Among those, immune play dual role during onset progression, as they can protect from killing immunogenic neoplastic cells, but in meanwhile also shape immunogenicity, contributing to escape. The interplay TME influences outcome immunotherapy many other anti-cancer therapies. Herein, we present an updated view pro- anti-tumor activities main cell populations breast TME, such T NK myeloid innate lymphoid mast eosinophils, underlying cytokine-, cell-cell contact- microvesicle-based mechanisms. Moreover, current novel therapeutic options revert immunosuppressive activity will be discussed. To this end, clinical trials assessing efficacy CAR-T CAR-NK vaccination, death-inducing chemotherapy, DNA methyl transferase histone deacetylase inhibitors, cytokines or their inhibitors immunotherapies patients reviewed. knowledge elapses experimental therapies targeting it, would help develop new combination treatments able overcome evasion mechanisms optimize benefit immunotherapies.

Language: Английский

Citations

183
Paradoxical effects of chemotherapy on tumor relapse and metastasis promotion DOI Creative Commons
Crescenzo D’Alterio, Stefania Scala, Gabriella Sozzi

et al.

Seminars in Cancer Biology, Journal Year: 2019, Volume and Issue: 60, P. 351 - 361

Published: Aug. 24, 2019

Several lines of compelling pre-clinical evidence identify chemotherapy as a potentially double-edged sword: therapeutic efficacy on the primary tumor may in fact be counterbalanced by induction tumor/host reactive responses supportive for survival and dissemination cancer cell subpopulations. This paradoxical effect can affect different districts such tumor, circulation distant organs simultaneously shaping properties composition stromal cells. At site, has been reported to promote selection chemoresistant disseminating cells endowed with stem (CSCs) through activation autocrine paracrine self-renewing/survival pathways promoted jointly therapy-selected Resistant CSCs represent seeds relapse increased infiltration immune cells, together enhanced vascular permeability induced chemotherapy, facilitates intravasation, first step metastatic cascade. As consequence tumor/metastasis re-shaping circulating (CTCs) detected during therapy display shift towards more mesenchymal stem-like phenotype, conductive ability survive seed organs. host activate inflammatory that ultimately facilitate extravasation colonization. Finally, cooperation endothelial at perivascular niches favors high potential metastasis initiation protects them from chemotherapy. review highlights pro-metastatic effects linking treatment formation remodeling generation favorable niche.

Language: Английский

Citations

182
Cancer stem cell secretome in the tumor microenvironment: a key point for an effective personalized cancer treatment DOI Creative Commons
Julia López de Andrés, Carmen Griñán‐Lisón, Gema Jiménez

et al.

Journal of Hematology & Oncology, Journal Year: 2020, Volume and Issue: 13(1)

Published: Oct. 15, 2020

Abstract Cancer stem cells (CSCs) represent a tumor subpopulation responsible for metastasis and resistance to chemo- radiotherapy, ultimately leading relapse. As consequence, the detection eradication of this cell current challenge in oncology medicine. CSC phenotype is dependent on microenvironment (TME), which involves differentiated cells, as well different types, such mesenchymal endothelial fibroblasts immune system, addition extracellular matrix (ECM), composition ECM healthy tissues. CSCs regulate multiple cancer hallmarks through interaction with their environment by secreting vesicles including exosomes, soluble factors interleukins, cytokines, growth other metabolites TME. Through these factors, generate activate own niche recruiting stromal modulate angiogenesis, metastasis, antitumor treatments maintenance secretion IL-6, VEGF TGF-ß. Due strong influence secretome disease development, new therapies focus targeting communication networks eradicate prevent relapse drug resistance. This review summarizes first time main components how they mediate processes. Lastly, relevance development more precise personalized discussed.

Language: Английский

Citations

162
Exosomal miRNAs in tumor microenvironment DOI Creative Commons

Shiming Tan,

Longzheng Xia,

Pin Yi

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2020, Volume and Issue: 39(1)

Published: April 16, 2020

Tumor microenvironment (TME) is the internal environment in which tumor cells survive, consisting of cells, fibroblasts, endothelial and immune as well non-cellular components, such exosomes cytokines. Exosomes are tiny extracellular vesicles (40-160nm) containing active substances, proteins, lipids nucleic acids. carry biologically miRNAs to shuttle between TME, thereby affecting development. Tumor-derived exosomal induce matrix reprogramming creating a that conducive growth, metastasis, escape chemotherapy resistance. In this review, we updated role process TME reshaping.

Language: Английский

Citations

158
Liquid biopsy: Exosomal microRNAs as novel diagnostic and prognostic biomarkers in cancer DOI Creative Commons

K. Auxzilia Preethi,

Sushmaa Chandralekha Selvakumar, Kehinde Ross

et al.

Molecular Cancer, Journal Year: 2022, Volume and Issue: 21(1)

Published: Feb. 16, 2022

Abstract Background Detecting cancer at an early stage before clinical manifestation could be effective strategy to decrease mortality. Thus, identifying liquid biopsy biomarkers with high efficacy a promising approach for non-invasive diagnosis of cancer. Main text Liquid biopsies are increasingly used as supplement biopsy, it enables disease progression detected months and radiographic confirmation. Many bodily fluids contain exosomal microRNAs (miRNAs) which provide new class minimally invasive due the stability miRNAs in exosomes. In this review, we mainly focused on (liquid biopsy) prognosis various cancers. Conclusion Exosomal can diagnostic that unique insights more dynamic perspective therapeutic responses malignancies. Therefore, development novel sensitive technologies exploit should priority management.

Language: Английский

Citations

132
Extracellular vesicles in ovarian cancer chemoresistance, metastasis, and immune evasion DOI Creative Commons

Wanjia Tian,

Ningjing Lei, Junying Zhou

et al.

Cell Death and Disease, Journal Year: 2022, Volume and Issue: 13(1)

Published: Jan. 18, 2022

Abstract Chemoresistance and metastasis are the major challenges for current ovarian cancer treatment. Understanding mechanisms of progression is critically important developing novel therapies. The advances in extracellular vesicles (EVs) research recent years have attracted extensive attention. EVs contain a variety proteins, RNAs, DNAs, metabolites. Accumulating evidence indicates that cells secrete large amount EVs, playing an role tumor recurrence. In microenvironment tumor, participate information transmission between stromal immune cells, promoting escape facilitating metastasis. Here, we review chemoresistance, metastasis, evasion cancer. Furthermore, also discuss EV future application as promising biomarker sources response to therapy therapy-delivery approaches patients.

Language: Английский

Citations

98