Solid-liquid partitioning of Dengue, West Nile, Zika, Hepatitis A, Influenza A, and SARS-CoV-2 viruses in wastewater from across the United States DOI Open Access
Laura Roldan-Hernandez, Camila Van Oost, Alexandria B. Boehm

et al.

Published: April 3, 2024

Limited information is available on the fate of respiratory and arthropod-borne viruses in wastewater. Enteric have been extensively studied wastewater treatment plants, however partition coefficients not well documented. This essential for interpreting wastewater-based surveillance (WBS) data optimizing sample collection processing methods. In this study, we examined solid-liquid partitioning behavior Dengue, West Nile, Zika, Hepatitis A, Influenza SARS-CoV-2 Samples were collected from primary sludge line eleven plants across United States spiked with varying concentrations each virus. Solid liquid fractions separated via centrifugation. Viral nucleic acids extracted quantified using reverse-transcription digital droplet PCR (RT-ddPCR). Partition (KF), determined Freundlich adsorption model, ranged 4.0✕10^2 mL/g to 3.9✕10^6 (median = 1.1✕10^4 mL/g). KF values significantly different between viruses. However, significant differences observed Zika Nile Virus plants. Further research needed understand how characteristics might impact viral markers. The results experiment underscore importance considering solids early detection monitoring infectious diseases, particularly regions low prevalence infections.

Language: Английский

Wastewater Detection of Emerging Arbovirus Infections: Case Study of Dengue in the United States DOI Creative Commons
Marlene K. Wolfe,

Abigail Harvey Paulos,

Alessandro Zulli

et al.

Environmental Science & Technology Letters, Journal Year: 2023, Volume and Issue: 11(1), P. 9 - 15

Published: Dec. 11, 2023

Climate change and urbanization are increasing the distribution of insect vectors infectious diseases. Dengue virus is an arbovirus that causes nearly 100 million symptomatic infections per year endemic in 124 countries, range its mosquito continues to increase. Surveillance dengue complicated by fact can be asymptomatic, symptoms may not readily recognizable clinicians. Here we show wastewater monitoring used detect RNA yield information about circulation a community. We collected three samples solids week from different treatment plants Miami-Dade County, Florida, where were locally acquired. Using molecular methods, tested for 4 serotypes consistently detected 3 at all did other serotypes. According publicly available data on infections, vast majority caused serotype 3. Wastewater detection possible with as few 4.23 laboratory confirmed cases 1 people based infection data.

Language: Английский

Citations

29
A retrospective longitudinal study of adenovirus group F, norovirus GI and GII, rotavirus, and enterovirus nucleic acids in wastewater solids at two wastewater treatment plants: solid-liquid partitioning and relation to clinical testing data DOI Creative Commons
Alexandria B. Boehm,

Bridgette Shelden,

Dorothea Duong

et al.

mSphere, Journal Year: 2024, Volume and Issue: 9(3)

Published: Feb. 27, 2024

Enteric infections are important causes of morbidity and mortality, yet clinical surveillance is limited. Wastewater-based epidemiology (WBE) has been used to study community circulation individual enteric viruses panels respiratory diseases, but there limited work studying the concurrent a suite viruses. A retrospective WBE was carried out at two wastewater treatment plants located in California, United States. Using digital droplet polymerase chain reaction (PCR), we measured concentrations human adenovirus group F, enteroviruses, norovirus genogroups I II, rotavirus nucleic acids solids times per week for 26 months (

Language: Английский

Citations

13
Observations of Respiratory Syncytial Virus (RSV) Nucleic Acids in Wastewater Solids Across the United States in the 2022–2023 Season: Relationships with RSV Infection Positivity and Hospitalization Rates DOI Creative Commons
Alessandro Zulli, Meri R.J. Varkila, Julie Parsonnet

et al.

ACS ES&T Water, Journal Year: 2024, Volume and Issue: 4(4), P. 1657 - 1667

Published: Feb. 29, 2024

Respiratory syncytial virus (RSV) is a leading cause of respiratory illness and hospitalization, but clinical surveillance detects only minority cases. Wastewater could determine the onset extent RSV circulation in absence sensitive case detection, to date, studies wastewater are few. We measured RNA concentrations solids from 176 sites during 2022–2023 season compared those publicly available infection positivity hospitalization rates. Concentrations ranged undetectable 107 copies per gram. concentration aggregated at state national levels correlated with was determined using both rates independent algorithms for 14 states where data were start season. In 4 states, identified same week; 3 preceded onset, 7 occurred after onset. generally peaked week as peaked. Differences peaks versus may reflect inherent differences approaches.

Language: Английский

Citations

13
Solid–liquid partitioning of dengue, West Nile, Zika, hepatitis A, influenza A, and SARS-CoV-2 viruses in wastewater from across the USA DOI Creative Commons
Laura Roldan-Hernandez, Camila Van Oost, Alexandria B. Boehm

et al.

Environmental Science Water Research & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Limited information is available on the fate of respiratory and arthropod-borne viruses in wastewater.

Language: Английский

Citations

13
Assessment of seasonality and normalization techniques for wastewater-based surveillance in Ontario, Canada DOI Creative Commons
Hadi A. Dhiyebi, Joud Abu Farah, Heather Ikert

et al.

Frontiers in Public Health, Journal Year: 2023, Volume and Issue: 11

Published: Aug. 30, 2023

Introduction Wastewater-based surveillance is at the forefront of monitoring for community prevalence COVID-19, however, continued uncertainty exists regarding use fecal indicators normalization SARS-CoV-2 virus in wastewater. Using three communities Ontario, sampled from 2021–2023, seasonality a viral indicator (pepper mild mottle virus, PMMoV) and utility data to improve correlations with clinical cases was examined. Methods Wastewater samples Warden, Humber Air Management Facility (AMF), Kitchener were analyzed SARS-CoV-2, PMMoV, crAssphage. The PMMoV flow rates examined compared by Season-Trend-Loess decomposition analysis. effects using crAssphage, comparing episode date (CBED) during 2021. Results Seasonal analysis demonstrated that had similar trends AMF peaks January April 2022 low concentrations (troughs) summer months. Warden but more sporadic between troughs concentrations. Flow not correlated very weak ( r = 0.12). Despite differences among sewersheds, unnormalized (raw N1–N2) concentration wastewater n 99–191) strongly CBED 0.620–0.854) Additionally, did significantly reduced Kitchener. crAssphage 29–57) all sites different raw N1–N2 CBED. Discussion Differences seasonal biomarkers caused sewershed characteristics (flow, input, etc.) may play role determining how effective be improving (or not). This study highlights importance assessing influence on normalized or other viruses concern. Fecal used normalize target interest help hinder establishing outcomes wastewater-based needs considered carefully across seasons sites.

Language: Английский

Citations

19
Adapted methods for monitoring influenza virus and respiratory syncytial virus in sludge and wastewater DOI Creative Commons
Daniel Toribio-Avedillo, Clara Gómez-Gómez, Laura Sala‐Comorera

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 918, P. 170636 - 170636

Published: Feb. 6, 2024

Wastewater-based surveillance constitutes a valuable methodology for the continuous monitoring of viral circulation, with capacity to function as an early warning system. It holds particular significance in scenarios where respiratory viruses exhibit overlapping clinical presentations, occurs SARS-CoV-2, influenza virus (IV), and syncytial (RSV), allows seasonal outbreaks be distinguished from COVID-19 peaks. Furthermore, sewage sludge, given it harbors concentrated human waste large population, serves substantial reservoir pathogen detection. To effectively integrate wastewater-based epidemiology into infectious disease surveillance, detection methods employed wastewater samples must adapted distinct characteristics sludge matrices. In this study, we applied protocols IV RSV comparing their performance results obtained wastewater. assess efficiency these protocols, were spiked RNA, either free or incorporated lentiviral particles. Samples using aluminum hydroxide adsorption-precipitation method before RNA extraction. Absolute quantification was carried out by RT-qPCR, including internal control monitor potential inhibitory factors. Recovery efficiencies both 60 % 75 71 respectively wastewater, whereas values particles 16 10 21 17 respectively. Additionally, protocol enabled naturally occurring collected two treatment plants during winter months, thus affirming efficacy methodologies.

Language: Английский

Citations

8
Persistence of human respiratory viral RNA in wastewater-settled solids DOI Creative Commons
Mengyang Zhang, Laura Roldan-Hernandez, Alexandria B. Boehm

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(4)

Published: March 19, 2024

Wastewater-based epidemiology has emerged as a valuable tool for monitoring respiratory viral diseases within communities by analyzing concentrations of nucleic-acids in wastewater. However, little is known about the fate virus Two important processes that may modulate their wastewater they move from household drains to point collection include sorption or partitioning solids and degradation. This study investigated decay kinetics genomic seven human viruses, including severe acute syndrome coronavirus 2 (SARS-CoV-2), syncytial (RSV), (HCoV)-OC43, HCoV-229E, HCoV-NL63, rhinovirus (HRV), influenza A (IAV), well pepper mild mottle (PMMoV) solids. Viruses (except PMMoV) were spiked into followed 50 days at three different temperatures (4°C, 22°C, 37°C). Viral RNA decayed following first-order with rate constants

Language: Английский

Citations

8
Prospective study of Candida auris nucleic acids in wastewater solids in 190 wastewater treatment plants in the United States suggests widespread occurrence DOI Creative Commons
Alessandro Zulli, Elana M. G. Chan,

Bridgette Shelden

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(8)

Published: July 23, 2024

is an emerging, multidrug-resistant fungal pathogen that poses a significant public health threat in healthcare settings. Despite yearly clinical cases rapidly increasing from 77 to 8,131 the last decade, surveillance data on its distribution and prevalence remain limited. We implemented novel assay for

Language: Английский

Citations

6
Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States DOI Creative Commons
Alexandria B. Boehm, Marlene K. Wolfe, Amanda L. Bidwell

et al.

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Oct. 17, 2024

We measured concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants, influenza A B viruses, syncytial virus, human metapneumovirus, enterovirus D68, parainfluenza types 1, 2, 3, 4a, 4b in aggregate, norovirus genotype II, rotavirus, Candida auris, hepatitis adenovirus, mpox H5 pepper mild mottle virus nucleic acids wastewater solids prospectively at 191 treatment plants 40 states across the United States plus Washington DC. Measurements were made two to seven times per week from 1 January 2022 30 June 2024, depending on plant staff availability. using droplet digital (reverse-transcription-) polymerase chain reaction (ddRT-PCR) following best practices for making environmental molecular biology measurements. These data can be used better understand disease occurrence communities contributing wastewater.

Language: Английский

Citations

6
Multiplexed Detection, Partitioning, and Persistence of Wild-Type and Vaccine Strains of Measles, Mumps, and Rubella Viruses in Wastewater DOI
Jingjing Wu, Michael Wang, Prashant Kalvapalle

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Wastewater surveillance of vaccine-preventable diseases may provide early warning outbreaks and identify areas to target for immunization. To advance wastewater monitoring measles, mumps, rubella viruses, we developed validated a multiplexed RT-ddPCR assay the detection their RNA. Because measles-mumps-rubella (MMR) vaccine is an attenuated live virus vaccine, also that distinguishes between wild-type strains measles in it using sample collected from facility with active outbreak. We evaluated partitioning behavior viruses liquid solid fractions influent wastewater. found assaying fraction resulted more sensitive despite fact viral RNA was enriched due low solids content Finally, investigated stability samples spiked over 28 days at two different concentrations temperatures (4 °C room temperature) through freeze-thaw observed limited decay. Our study supports feasibility population-level surveillance.

Language: Английский

Citations

4