Engineered Extracellular Vesicles in Wound Healing: Design, Paradigms, and Clinical Application DOI
Yuheng Liao, Zhenhe Zhang,

Lizhi Ouyang

et al.

Small, Journal Year: 2023, Volume and Issue: 20(7)

Published: Oct. 8, 2023

Abstract The severe quality of life and economic burden imposed by non‐healing skin wounds, infection risks, treatment costs are affecting millions patients worldwide. To mitigate these challenges, scientists relentlessly seeking effective measures. In recent years, extracellular vesicles (EVs) have emerged as a promising cell‐free therapy strategy, attracting extensive attention from researchers. EVs mediate intercellular communication, possessing excellent biocompatibility stability. These features make potential tool for treating plethora diseases, including those related to wound repair. However, there is growing focus on the engineering overcome inherent limitations such low production, relatively fixed content, targeting capabilities natural EVs. This could improve both effectiveness specificity in repair treatments. light this, present review will introduce latest progress design methods experimental paradigms engineered applied Furthermore, it comprehensively analyze current clinical research status prospects within this field.

Language: Английский

Porous microneedle patch with sustained delivery of extracellular vesicles mitigates severe spinal cord injury DOI Creative Commons

Ao Fang,

Yifan Wang,

Naiyu Guan

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 7, 2023

Abstract The transplantation of mesenchymal stem cells-derived secretome, particularly extracellular vesicles is a promising therapy to suppress spinal cord injury-triggered neuroinflammation. However, efficient delivery the injured cord, with minimal damage, remains challenge. Here we present device for treat injury. We show that incorporating cells and porous microneedles enables vesicles. demonstrate topical application lesion beneath dura, does not damage lesion. evaluate efficacy our in contusive injury model find it reduces cavity scar tissue formation, promotes angiogenesis, improves survival nearby tissues axons. Importantly, sustained at least 7 days results significant functional recovery. Thus, provides an platform treatment.

Language: Английский

Citations

58
Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury DOI
Weidong Wu, Shuaijun Jia, Hailiang Xu

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(4), P. 3818 - 3837

Published: Feb. 14, 2023

Neural stem cells (NSCs) are considered to be prospective replacements for neuronal cell loss as a result of spinal cord injury (SCI). However, the survival and differentiation NSCs strongly affected by unfavorable microenvironment induced SCI, which critically impairs their therapeutic ability treat SCI. Herein, strategy fabricate PDGF-MP hydrogel (PDGF-MPH) microspheres (PDGF-MPHM) instead bulk hydrogels is proposed dramatically enhance efficiency platelet-derived growth factor mimetic peptide (PDGF-MP) in activating its receptor. PDGF-MPHM were fabricated piezoelectric ceramic-driven thermal electrospray device, had an average size 9 μm, also activate PDGFRβ more effectively than PDGF-MPH. In vitro, exerted strong neuroprotective effects maintaining proliferation inhibiting apoptosis presence myelin extracts. vivo, inhibited M1 macrophage infiltration extrinsic or intrinsic on seventh day after Eight weeks T10 SCI treatment results showed that + significantly promoted differentiation, reduced lesion size, considerably improved motor function recovery rats stimulating axonal regeneration, synapse formation, angiogenesis comparison with graft group. Therefore, our findings provide insights into promising agent repair.

Language: Английский

Citations

50
Injectable hydrogels as promising in situ therapeutic platform for cartilage tissue engineering DOI
Rong Cai,

Yisi Shan,

F. Du

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 261, P. 129537 - 129537

Published: Jan. 25, 2024

Language: Английский

Citations

19
Neural stem cell-derived exosomes and regeneration: cell-free therapeutic strategies for traumatic brain injury DOI Creative Commons
Lin Zhong, Jingjing Wang, Peng Wang

et al.

Stem Cell Research & Therapy, Journal Year: 2023, Volume and Issue: 14(1)

Published: Aug. 8, 2023

Abstract Regenerative repair of the brain after traumatic injury (TBI) remains an extensive clinical challenge, inspiring intensified interest in therapeutic approaches to explore superior strategies. Exosome therapy is another research hotspot following stem cell alternative therapy. Prior verified that exosomes produced by neural cells can participate physiological and pathological changes associated with TBI have potential neuroregulatory functions. In comparison their parental cells, stability immune tolerance lower tumorigenic risk. addition, they readily penetrate blood‒brain barrier, which makes treatment efficiency transplanted cells. Exosomes secreted present a promising strategy for development novel regenerative therapies. Their tissue regeneration immunomodulatory made them encouraging candidates repair. The review addresses challenges, applications mechanisms regenerating damaged brains.

Language: Английский

Citations

40
Hypoxia endothelial cells-derived exosomes facilitate diabetic wound healing through improving endothelial cell function and promoting M2 macrophages polarization DOI Creative Commons
Cheng Peng, Xudong Xie,

Liangcong Hu

et al.

Bioactive Materials, Journal Year: 2023, Volume and Issue: 33, P. 157 - 173

Published: Nov. 10, 2023

It is imperative to develop and implement newer, more effective strategies address refractory diabetic wounds. As of now, there currently no optimal solution for these Hypoxic human umbilical vein endothelial cells (HUVECs)-derived exosomes have been postulated promote wound healing, however, its effect molecular mechanism need further study. In this study, we aimed investigate whether hypoxic enhance healing in diabetics. Based on our high-throughput sequencing, differentially expressed lncRNAs (including 64 upregulated 94 downregulated lncRNAs) were found compared normoxic exosomes. Interestingly, lncHAR1B was one the prominently exosomes, showing a notable correlation with healing. More specifically, transmitted surrounding cells, which resulted significant increase level, thereby relieving dysfunction promoting switch from M1 M2 macrophages under high glucose conditions. Mechanistically, directly interacted transcription factor basic helix-loop-helix family member e23 (BHLHE23), subsequently led binding KLF 4 (KLF4) promoted KLF4 expression.

Language: Английский

Citations

40
Wearable and implantable biosensors: mechanisms and applications in closed-loop therapeutic systems DOI Creative Commons
Zeyuan Zheng,

Runjin Zhu,

Ian Peng

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(35), P. 8577 - 8604

Published: Jan. 1, 2024

Biosensors integrated into closed-loop systems provide patient-specific adaptive therapies. These advanced devices have significant potential for managing chronic conditions by continuously adjusting therapeutic regimens based on physiological data.

Language: Английский

Citations

10
Plant‐derived exosomes extracted from Lycium barbarum L. loaded with isoliquiritigenin to promote spinal cord injury repair based on 3D printed bionic scaffold DOI Creative Commons
Qilong Wang, Kai Liu, Xia Cao

et al.

Bioengineering & Translational Medicine, Journal Year: 2024, Volume and Issue: 9(4)

Published: Jan. 30, 2024

Abstract Plant‐derived exosomes (PEs) possess an array of therapeutic properties, including antitumor, antiviral, and anti‐inflammatory capabilities. They are also implicated in defensive responses to pathogenic attacks. Spinal cord injuries (SCIs) regeneration represents a global medical challenge, with appropriate research concentration on three pivotal domains: neural promotion, inflammation inhibition, innovation application regenerative scaffolds. Unfortunately, the utilization PE SCI therapy remains unexplored. Herein, we isolated from traditional Chinese medicinal herb, Lycium barbarum L. discovered their inflammatory inhibition neuronal differentiation promotion Compared derived ectomesenchymal stem cells (EMSCs), demonstrated substantial enhancement differentiation. We encapsulated isoliquiritigenin (ISL)‐loaded plant‐derived (ISL@PE) within 3D‐printed bionic scaffold. The intricate construct modulated response following SCI, facilitating restoration damaged axons culminating ameliorated neurological function. This pioneering investigation proposes novel potential route for insoluble drug delivery via plant exosomes, as well repair. institutional animal care use committee number is UJS‐IACUC‐2020121602.

Language: Английский

Citations

8
Enhancing intraneural revascularization following peripheral nerve injury through hypoxic Schwann-cell-derived exosomes: an insight into endothelial glycolysis DOI Creative Commons
Jun Sun,

Qiuhua Zeng,

Zhimin Wu

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 24, 2024

Abstract Background Endothelial cell (EC)-driven intraneural revascularization (INRV) and Schwann cells-derived exosomes (SCs-Exos) both play crucial roles in peripheral nerve injury (PNI). However, the interplay between them remains unclear. We aimed to elucidate effects underlying mechanisms of SCs-Exos on INRV following PNI. Results found that GW4869 inhibited INRV, as well normoxic (N-SCs-Exos) exhibited significant pro-INRV vivo vitro were potentiated by hypoxic (H-SCs-Exos). Upregulation glycolysis emerged a pivotal factor for after PNI, evidenced observation 3PO administration, glycolytic inhibitor, process vitro. H-SCs-Exos more significantly enhanced extracellular acidification rate/oxygen consumption rate ratio, lactate production, gene expression while simultaneously suppressing acetyl-CoA production pyruvate dehydrogenase E1 subunit alpha (PDH-E1α) than N-SCs-Exos Furthermore, we determined enriched with miR-21-5p N-SCs-Exos. Knockdown attenuated pro-glycolysis H-SCs-Exos. Mechanistically, orchestrated EC metabolism favor targeting von Hippel-Lindau/hypoxia-inducible factor-1α PDH-E1α, thereby enhancing hypoxia-inducible factor-1α-mediated inhibiting PDH-E1α-mediated oxidative phosphorylation. Conclusion This study unveiled novel intrinsic mechanism providing promising therapeutic target post-injury regeneration repair. Graphical

Language: Английский

Citations

8
Recent Advances in the Application of Engineered Exosomes from Mesenchymal Stem Cells for Regenerative Medicine DOI
Reza Abbasi, Ghazal Alamdari-Mahd, Hadi Maleki

et al.

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 177236 - 177236

Published: Jan. 1, 2025

Language: Английский

Citations

1
Barrier-penetrating liposome targeted delivery of basic fibroblast growth factor for spinal cord injury repair DOI Creative Commons

Fenzan Wu,

Penghui Wang,

Xiaojie Wei

et al.

Materials Today Bio, Journal Year: 2023, Volume and Issue: 18, P. 100546 - 100546

Published: Jan. 7, 2023

Nanoparticle technologies offer a non-invasive means to deliver basic fibroblast growth factor (bFGF) for the treatment of spinal cord injury (SCI). However, inability bFGF accumulate at site and inefficient penetration across blood-spinal barrier (BSCB) remain challenges. The present study describes dual-targeting liposome (bFGF@Lip-Cp&Rp) with lesion targeting BSCB-penetrating capability SCI treatment. CAQK peptide (Cp) ability R2KC (Rp) were grafted onto liposomes flexible drug delivery systems preparation. Results exhibit that dual-targeted could significantly cross BSCB site. During early stage SCI, bFGF@Lip-Cp&Rp promotes repair facilitates M2-polarization macrophages. Regular increase HUVECs tube formation angiogenesis, ameliorate microenvironment site, suppress neuronal apoptosis axonal atrophy in rats. Importantly, continuous supports restoration limb motor function In summary, this research implies site-targeting be promising therapeutic approach SCI.

Language: Английский

Citations

21