Mussel-Inspired MXene/Antimicrobial Peptide-Integrated Photosensitive Poly(vinyl alcohol)-Based Hydrogel with Antibacterial, Anti-Inflammatory, and Electroactive Properties for Accelerated Wound Healing DOI
Yu Sun, Qingping Liu, Zhenglei Yu

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Backgrounds: The buildup of reactive oxygen species (ROS) in infected wounds triggers an excessive inflammatory response, while the overuse antibiotics has contributed to increased bacterial resistance. Therefore, developing wound dressings that effectively eliminate ROS and inhibit growth is crucial. Methods: Inspired by mussel-derived proteins, we developed a polydopamine (PDA)-grafted MXene (PDA@MXene) 3,4-dihydroxyphenylalanine-PonG1 (DOPA-PonG1)-modified photosensitive poly(vinyl alcohol) (PVA) hydrogel as dressing. PDA@MXene was synthesized through dopamine self-polymerization on surface, tyrosine hydroxylation used introduce DOPA into antibacterial peptide ponericin G1 (PonG1). its components were characterized, their morphology examined. hydrogel's hemostatic ability, mechanical properties, conductivity evaluated. In vitro studies systematically evaluated antioxidative effects, activity, biocompatibility, expression tissue regeneration-related factors. An full-thickness skin defect model established vivo, different treatments applied. wound-healing rate then measured, followed histological analysis using hematoxylin eosin, Masson, Sirius Red, immunofluorescence staining investigate healing mechanism. Results: sequence enhanced PonG1 stability leading sustained ability. significantly improved strength. Notably, combined effects DOPA-PonG1 ROS-scavenging properties. vivo findings demonstrated DOPA-PonG1/PDA@MXene/PVA accelerated promoting angiogenesis collagen deposition reducing inflammation. This study presents innovative approach for treating defects holds promise clinical applications.

Language: Английский

M2 macrophage-polarized anti-inflammatory microneedle patch for accelerating biofilm-infected diabetic wound healing via modulating the insulin pathway DOI Creative Commons
Yushan Yang, Limin Fan,

Jingsi Jiang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Aug. 14, 2024

Macrophages play a pivotal role in the healing of diabetic ulcers. The sustained elevation glucose levels damages insulin signaling pathway macrophages, leading to dysfunctional macrophages that struggle transition from pro-inflammatory (M1) reparative (M2) states. Therefore, modulating macrophage inflammatory responses via holds promise for ulcer treatment. Additionally, presence biofilm impedes drug penetration, and resulting immunosuppressive microenvironment exacerbates persistent infiltration M1 macrophages. we designed an array dissolvable microneedle (denoted as NPF@MN) loaded with self-assembled nanoparticles could deliver NPF nanoparticles, acid-sensitive NPF-releasing Protocatechualdehyde (PA) hypoglycemic insulin-like effects, regulating polarization anti-inflammatory M2 phenotype. this study extensively examined mechanism by which NPF@MN accelerates ulcers through activation pathway. Through RNA-seq GSEA analysis, identified reduction expression pathway-related factors such IR, IRS-1, IRS-2, SHC. Our work presents innovative therapeutic approach targeting underscores its translational potential clinical management.

Language: Английский

Citations

19
Engineered MXene Biomaterials for Regenerative Medicine DOI

Shengmin Zhang,

Liang Wang, Zhichao Feng

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

MXene-based materials have attracted significant interest due to their distinct physical and chemical properties, which are relevant fields such as energy storage, environmental science, biomedicine. MXene has shown potential in the area of tissue regenerative medicine. However, research on its applications regeneration is still early stages, with a notable absence comprehensive reviews. This review begins detailed description intrinsic properties MXene, followed by discussion various nanostructures that can form, spanning from 0 3 dimensions. The focus then shifts biomaterials engineering, particularly immunomodulation, wound healing, bone regeneration, nerve regeneration. MXene's physicochemical including conductivity, photothermal characteristics, antibacterial facilitate interactions different cell types, influencing biological processes. These highlight modulating cellular functions essential for Although developing, versatile structural attributes suggest role advancing

Language: Английский

Citations

6
Bioinspired Adhesive Hydrogel Platform with Photothermal Antimicrobial, Antioxidant, and Angiogenic Properties for Whole-Process Management of Diabetic Wounds DOI

Peirong Zhou,

Chuankai Zhang,

Zihan Rao

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Diabetic wound healing remains a major challenge in modern medicine. The persistent inflammation and immune dysfunction hinder angiogenesis by producing excessive ROS increasing the susceptibility to bacterial infection. In this study, we developed an integrated strategy for whole-process management of diabetic wounds based on bioinspired adhesive hydrogel platform with hemostasis, photothermal antimicrobial, antioxidant, anti-inflammatory, angiogenic properties. A composite (termed AQTGF) using poly(acrylic acid) (PAA) quaternized chitosan (QCS) as backbone materials loaded TA-Gd/Fe-bimetallic-phenolic coordination polymer was prepared. AQTGF displayed favorable mechanical properties, self-healing capabilities, adhesion characteristics, response performance. vitro experiments demonstrated that exhibits excellent antimicrobial capacity angiogenic, M2 macrophage phenotype polarizing addition, rat tail amputation liver hemostasis had Moreover, vivo studies have indicated can facilitate accelerating epidermal growth, promoting collagen deposition, modulating polarization, inhibiting inflammation, angiogenesis. conclusion, study provides adaptable holds promise treatment chronic wounds.

Language: Английский

Citations

3
Antioxidant and Immunomodulatory Polymer Vesicles for Effective Diabetic Wound Treatment through ROS Scavenging and Immune Modulating DOI
Wenqing Zhang, Zhenghong Ge, Yufen Xiao

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(31), P. 9494 - 9504

Published: July 26, 2024

Chronic diabetic wound patients usually show high glucose levels and systemic immune disorder, resulting in reactive oxygen species (ROS) cell dysfunction, prolonged inflammation, delayed healing. Herein, we prepared an antioxidant immunomodulatory polymer vesicle for treatment. This is self-assembled from poly(ε-caprolactone)36-block-poly[lysine4-stat-(lysine-mannose)22-stat-tyrosine)16] ([PCL36-b-P[Lys4-stat-(Lys-Man)22-stat-Tyr16]). Polytyrosine polypeptide that can scavenge ROS. d-Mannose was introduced to afford functions by promoting macrophage transformation Treg activation through inhibitory cytokines. The mice treated with vesicles showed 23.7% higher a 91.3% M2/M1 ratio than those PBS. Animal tests confirmed this accelerated healing achieved complete of S. aureus-infected wounds within 8 days. Overall, the first scavenging ROS regulating homeostasis, opening new avenues effective

Language: Английский

Citations

14
Two-dimensional nanomaterials: A multifunctional approach for robust for diabetic wound repair DOI Creative Commons
Mingming Cui, Jin Z. Zhang, Pengfei Han

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 28, P. 101186 - 101186

Published: Aug. 6, 2024

Diabetic wounds pose a clinical challenge due to persistent inflammation, severe bacterial infections, inadequate vascularization, and pronounced oxidative stress. Current therapeutic modalities fail provide satisfactory outcomes in managing these conditions, resulting considerable patient distress. Two-dimensional nanomaterials (2DNMs), characterized by their unique nanosheet structures, expansive surface areas, remarkable physicochemical properties, have garnered attention for potential applications. Emerging 2DNMs can be loaded with various pharmacological agents, including small molecules, metal ions, liposomes. Moreover, they integrated biomaterials such as hydrogels, microneedles, microspheres, thus demonstrating unprecedented advantages expediting the healing process of diabetic wounds. exhibit exceptional performance characteristics, high biocompatibility, effective antimicrobial optimal phototherapeutic effects, enhanced electrostimulation capabilities. These properties enable them modulate wound microenvironment, leading widespread application tissue repair outcomes. This review delineates several emerging 2DNMs, graphene its derivatives, black phosphorus, MXenes, transition dichalcogenides, context repair. Furthermore, it elucidates translational challenges future perspectives treatments. Overall, present highly promising strategy ameliorating wounds, providing novel avenues diagnostic strategies management.

Language: Английский

Citations

12
Co-Assembled Binary Polyphenol Natural Products for the Prevention and Treatment of Radiation-Induced Skin Injury DOI
Yanwei Hu,

Yuhang Miao,

Yan Zhang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Radiation therapy, a fundamental treatment for tumors, is often accompanied by radiation-induced skin injury (RISI). Excessive production of reactive oxygen species (ROS) and subsequent inflammation are two key factors in RISI development that will cause affect radiotherapy. Herein, the co-assembled binary polyphenol natural products inspired dual-functional cascade microneedle system prevention RISI. Specifically, epigallocatechin gallate (EGCG) curcumin (CUR) were into nanoparticles (CEPG) intermolecular interactions then incorporated with catalase (CAT) to achieve microneedles (this was conducive penetrate epidermal keratinocytes where had greatest impact). When using microneedles, tip dissolved rapidly delivered CEPG CAT dermis, NPs able respond ROS decompose EGCG CUR. More importantly, formed converts superoxide anions water step-by-step, which can reduce cell damage caused free radicals early stages radiation prevention; meanwhile, CUR inhibited inflammatory pathways, achieving post-radiotherapy period. These explorations broaden strategy application

Language: Английский

Citations

11
Zinc-based Polyoxometalate Nanozyme Functionalized Hydrogels for optimizing the Hyperglycemic-Immune Microenvironment to Promote Diabetic Wound Regeneration DOI Creative Commons

Chaoyu Pu,

Yong Wang,

Honglin Xiang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 8, 2024

In diabetic wounds, hyperglycemia-induced cytotoxicity and impaired immune microenvironment plasticity directly hinder the wound healing process. Regulation of hyperglycemic remodeling are crucial.

Language: Английский

Citations

10
Microenvironment-responsive nanomedicines: a promising direction for tissue regeneration DOI Creative Commons
Yuan Xiong, Bobin Mi, Mohammad‐Ali Shahbazi

et al.

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: Oct. 21, 2024

Abstract Severe tissue defects present formidable challenges to human health, persisting as major contributors mortality rates. The complex pathological microenvironment, particularly the disrupted immune landscape within these defects, poses substantial hurdles existing regeneration strategies. However, emergence of nanobiotechnology has opened a new direction in immunomodulatory nanomedicine, providing encouraging prospects for and restoration. This review aims gather recent advances nanomedicine foster regeneration. We begin by elucidating distinctive features local microenvironment defective tissues its crucial role Subsequently, we explore design functional properties nanosystems. Finally, address clinical translation development, aiming propose potent approach enhance through synergistic modulation integration.

Language: Английский

Citations

10
Exosome-transmitted LUCAT1 promotes stemness transformation and chemoresistance in bladder cancer by binding to IGF2BP2 DOI Creative Commons
Yonghao Zhan, Zhenzhen Zhou, Zhaowei Zhu

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2025, Volume and Issue: 44(1)

Published: March 3, 2025

Abstract The chemotherapy resistance is an awkward challenge in management of bladder cancer (BC). Cancer organoid model effective preclinical tumor that could faithfully represent clinical manifestations and simulate the biological processes chemoresistance. Recent studies have revealed stem cells (CSCs) play a significant role development chemoresistance cancer. Exosomes act as essential intercellular messengers participate controlling conversion distinct cell characteristics, including However, exosome-transmitted lncRNAs has rarely been reported. In this study, models were developed from urothelial carcinomas to explore pathophysiology mechanism BC chemoresistance, RNA-seq was performed screen for involved BC. We found enriches stem-like BC, upregulation Lung Associated Transcript 1 (LUCAT1) occurs chemotherapy-resistant organoids correlated with response. Further experimental results demonstrated LUCAT1 promotes by enhancing stemness phenotype vivo vitro. Moreover, exosomes derived can enhance delivering LUCAT1. Mechanistically, significantly mRNA stability HMGA1 via binding IGF2BP2 m6A-dependent manner. study demonstrates important potential function both diagnostic biomarker therapeutic target

Language: Английский

Citations

2
Low-dimensional antimicrobial nanomaterials in anti-infection treatment and wound healing DOI

Yunfen Gao,

Liying Wang,

Chufan Zhou

et al.

Chinese Chemical Letters, Journal Year: 2024, Volume and Issue: unknown, P. 110028 - 110028

Published: May 1, 2024

Language: Английский

Citations

7