2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO),
Journal Year:
2023,
Volume and Issue:
unknown, P. 443 - 447
Published: July 31, 2023
The
electrochemical
detection
of
endogenous
molecules
from
a
single
cell
is
crucial
technique
for
understanding
fundamental
cellular
processes
at
the
subcellular
level
and
uncovering
heterogeneity.
However,
high
throughput
intracellular
has
posed
significant
challenges.
This
paper
proposes
simple
method
fabricating
nanoprobe
monitoring
reactive
oxygen
species
(ROS),
which
are
important
signaling
that
play
key
role
in
wide
range
processes,
including
apoptosis,
proliferation,
differentiation.
Combined
with
automated
platform,
study
successfully
achieved
minimally
invasive
efficient
ROS
living
cells.
Moreover,
results
reveal
average
concentration
cytoplasm
nucleus
uneven,
implications
redox
signaling.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(19), P. 7411 - 7420
Published: April 23, 2024
Accurate
analysis
of
microRNAs
(miRNAs)
at
the
single-cell
level
is
extremely
important
for
deeply
understanding
their
multiple
and
intricate
biological
functions.
Despite
some
advancements
in
analyzing
miRNAs,
challenges
such
as
intracellular
interferences
insufficient
detection
limits
still
remain.
In
this
work,
an
ultrasensitive
nanopore
sensor
quantitative
miRNA-155
constructed
based
on
ionic
current
rectification
(ICR)
coupled
with
enzyme-free
catalytic
hairpin
assembly
(CHA).
Benefiting
from
CHA
amplification
strategy,
limit
reaches
10
fM
more
adaptable
to
complex
environments.
With
sensor,
concentration
living
single
cells
quantified
realize
early
diagnosis
triple-negative
breast
cancer
(TNBC).
Furthermore,
can
be
applied
screening
anticancer
drugs
by
tracking
expression
miRNA-155.
This
work
provides
adaptive
universal
method
quantitatively
which
will
greatly
improve
our
cell
heterogeneity
provide
a
reliable
scientific
basis
exploring
major
diseases
level.
ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(7), P. 2583 - 2590
Published: June 27, 2023
Exosomes
secreted
from
cells
carry
rich
information
their
parent
cells,
representing
a
promising
biomarker
for
investigation
of
diseases.
We
develop
dual-nanopore
biosensor
using
DNA
aptamers
to
specifically
recognize
CD63
protein
on
the
exosome's
surface,
which
enables
label-free
exosome
detection
based
ionic
current
change.
The
sensor
allows
sensitive
exosomes
with
limit
3.4
×
106
particles/mL.
was
able
form
an
intrapipette
electric
circuit
measurement
due
its
unique
structure,
is
crucial
achieve
secretion
single
cell.
utilized
microwell
array
chip
entrap
cell
into
confined
small
volume,
enabling
accumulation
high
concentration.
positioned
cell,
and
monitoring
in
different
lines
under
stimulations
has
been
achieved.
Our
design
may
provide
useful
platform
developing
nanopore
biosensors
detecting
secretions
living
ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(9), P. 3428 - 3434
Published: Aug. 8, 2023
Pesticides
have
caused
concerns
about
food
safety
due
to
their
residual
effects
in
vegetables
and
fruits.
Imidacloprid,
as
the
frequently
used
neonicotinoid
pesticide,
could
harm
cardiovascular
respiratory
function
cause
reproductive
toxicity
humans.
Therefore,
reliable
methods
for
portable,
selective,
rapid
detection
are
desirable
develop.
Herein,
we
report
a
neuron-inspired
nanofluidic
biosensor
based
on
tyrosine-modified
artificial
nanochannel
sensitively
detecting
imidacloprid.
The
functional
tyrosine
is
modified
outer
surface
of
porous
anodic
aluminum
oxide
rapidly
capture
imidacloprid
through
π-π
interactions
hydrogen
bonds.
integrated
has
wide
concentration
range
from
10-8
10-4
g/mL
with
an
ultralow
limit
6.28
×
10-9
g/mL,
which
outperforms
state-of-the-art
sensors.
This
work
provides
new
perspective
residues
well
other
hazardous
pesticide
environmental
samples.
Chemistry - A European Journal,
Journal Year:
2024,
Volume and Issue:
30(30)
Published: March 20, 2024
Abstract
Solid‐state
glass
nanopipettes
provide
a
promising
confined
space
that
offers
several
advantages
such
as
controllable
size,
simple
preparation,
low
cost,
good
mechanical
stability,
and
thermal
stability.
These
make
them
an
ideal
choice
for
various
applications
biosensors,
DNA
sequencing,
drug
delivery.
In
this
review,
we
first
delve
into
the
functionalized
sensing
analytes
methods
used
to
develop
detection
means
with
them.
Next,
in‐depth
overview
of
advanced
functionalization
methodologies
based
on
diversified
chemical
kinetics.
After
that,
present
latest
state‐of‐the‐art
achievements
potential
in
detecting
wide
range
targets,
including
ions,
molecules,
biological
macromolecules,
single
cells.
We
examine
challenges
arise
when
working
these
well
innovative
solutions
developed
overcome
The
final
section
current
development
status,
newest
trends,
application
prospects
sensors.
Overall,
review
provides
comprehensive
detailed
analysis
nanopipette
perception
valuable
insights
exciting
field.
