Unraveling the Atomistic Mechanism of Electrostatic Lateral Association of Peptide β‐Sheet Structures and Its Role in Nanofiber Growth and Hydrogelation DOI Creative Commons
M.A. Soliman, Abdulwahhab Khedr, Tarsem Sahota

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Abstract Guiding molecular assembly of peptides into rationally engineered nanostructures remains a major hurdle against the development functional peptide‐based nanomaterials. Various non‐covalent interactions come play to drive formation and stabilization these assemblies, which electrostatic are key. Here, atomistic mechanisms by contribute toward controlling self‐assembly lateral association ultrashort β‐sheet forming deciphered. Our results show that this is governed charge distribution ionic complementarity, both affecting interaction patterns between charged residues: terminal, core, and/or terminal‐to‐core attraction/repulsion. Controlling enabled fine‐tuning nanofiber morphology for 16 examined peptides, resulting versatile ranging from extended thin fibrils thick bundles twisted helical “braids” short pseudocrystalline nanosheets. This in turn affected physical appearance viscoelasticity formed materials, varying turbid colloidal dispersions viscous solutions soft stiff self‐supportive hydrogels, as revealed oscillatory rheology. Atomistic were confirmed dynamic simulations, validating nanoscopic characterization developed materials. In essence, detailed emphasizing impact complementarity on self‐assembly, nanostructure formation, hydrogelation reported.

Language: Английский

Biomimetic peptide self-assembly for functional materials DOI
Aviad Levin, Tuuli A. Hakala, Lee Schnaider

et al.

Nature Reviews Chemistry, Journal Year: 2020, Volume and Issue: 4(11), P. 615 - 634

Published: Sept. 15, 2020

Language: Английский

Citations

659
Antimicrobial Peptides: A New Hope in Biomedical and Pharmaceutical Fields DOI Creative Commons
Antonio Moretta, Carmen Scieuzo,

Anna Maria Petrone

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2021, Volume and Issue: 11

Published: June 14, 2021

Antibiotics are essential drugs used to treat pathogenic bacteria, but their prolonged use contributes the development and spread of drug-resistant microorganisms. Antibiotic resistance is a serious challenge has led need for new alternative molecules less prone bacterial resistance. Antimicrobial peptides (AMPs) have aroused great interest as potential next-generation antibiotics, since they bioactive small proteins, naturally produced by all living organisms, representing first line defense against fungi, viruses bacteria. AMPs commonly classified according sources, which represented microorganisms, plants animals, well secondary structure, biosynthesis mechanism action. They find application in different fields such agriculture, food industry medicine, on we focused our attention this review. Particularly, examined AMP applicability wound healing, skin infections metabolic syndrome, considering ability act Angiotensin-Converting Enzyme I pancreatic lipase inhibitory antioxidant peptides. Moreover, argued about pharmacokinetic pharmacodynamic approaches develop drug strategies formulation be taken into account developing clinically suitable applications.

Language: Английский

Citations

378
Enhanced photocatalytic activity and photothermal effects of cu-doped metal-organic frameworks for rapid treatment of bacteria-infected wounds DOI

Donglin Han,

Yajing Han,

Jun Li

et al.

Applied Catalysis B Environment and Energy, Journal Year: 2019, Volume and Issue: 261, P. 118248 - 118248

Published: Oct. 3, 2019

Language: Английский

Citations

327
Natural Polymer-Based Antimicrobial Hydrogels without Synthetic Antibiotics as Wound Dressings DOI

Yajie Zhong,

Huining Xiao, Farzad Seidi

et al.

Biomacromolecules, Journal Year: 2020, Volume and Issue: 21(8), P. 2983 - 3006

Published: July 16, 2020

Wound healing is usually accompanied by bacterial infection. The excessive use of synthetic antibiotics leads to drug resistance, posing a significant threat human health. Hydrogel-based wound dressings aimed at mitigating infections have emerged as an effective treatment. review presented herein particularly focuses on the hydrogels originating from natural polymers. To further enhance performance dressings, various strategies and approaches been developed endow with excellent broad-spectrum antibacterial activity. Those that are summarized in current intrinsic or stimuli-triggered bactericidal properties others serve vehicles for loading agents without antibiotics. Specific attention paid antimicrobial mechanisms hydrogels. Practical applications accelerate employing these antibiotic-free also introduced along discussion challenges perspectives leading new technologies.

Language: Английский

Citations

287
Design, optimization, and nanotechnology of antimicrobial peptides: From exploration to applications DOI
Peng Tan,

Huiyang Fu,

Xi Ma

et al.

Nano Today, Journal Year: 2021, Volume and Issue: 39, P. 101229 - 101229

Published: July 5, 2021

Language: Английский

Citations

249
Multifunctional Antimicrobial Biometallohydrogels Based on Amino Acid Coordinated Self‐Assembly DOI Creative Commons
Jingwen Song, Chengqian Yuan, Tifeng Jiao

et al.

Small, Journal Year: 2020, Volume and Issue: 16(8)

Published: Jan. 29, 2020

Abstract There is a real need for new antibiotics against self‐evolving bacteria. One option to use biofriendly broad‐spectrum and mechanically tunable antimicrobial hydrogels that can combat multidrug‐resistant microbes. Whilst appealing, there are currently limited options. Herein, biometallohydrogels based on the self‐assembly local mineralization of Ag + ‐coordinated Fmoc‐amino acids reported. Such have advantages localized delivery sustained release, reduced drug dosage toxicity yet improved bioavailability, prolonged effect, mechanical strength. Furthermore, they directly interact with cell walls membrane, resulting in detachment plasma membrane leakage cytoplasm. This leads death, triggering significant antibacterial effect both Gram‐negative ( Escherichia coli ) Gram‐positive Staphylococcus aureus bacteria cells mice. study paves way developing multifunctional integration platform simple biomolecules coordinated toward broad range biomedical applications.

Language: Английский

Citations

230
Self‐Assembling Peptide‐Based Hydrogels for Wound Tissue Repair DOI Creative Commons
Tong Guan, Jiayang Li, Chunying Chen

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(10)

Published: Feb. 9, 2022

Wound healing is a long-term, multistage biological process that includes hemostasis, inflammation, proliferation, and tissue remodeling requires intelligent designs to provide comprehensive convenient treatment. The complexity of wounds has led lack adequate wound treatment materials, which must systematically regulate unique microenvironments. Hydrogels have significant advantages in due their ability spatiotemporal control over the process. Self-assembling peptide-based hydrogels are particularly attractive innate biocompatibility biodegradability along with additional including ligand-receptor recognition, stimulus-responsive self-assembly, mimic extracellular matrix. materials self-assemble response physiological environment, resulting functionalized microscopic structures, makes them conducive This review introduces several self-assembling systems various emphasizes recent advances allow for precise during different stages healing. Moreover, development multifunctional can remodel immune microenvironment therapy also been summarized. Overall, this sheds light on future clinical practical applications hydrogels.

Language: Английский

Citations

212
Local Photothermal/Photodynamic Synergistic Therapy by Disrupting Bacterial Membrane To Accelerate Reactive Oxygen Species Permeation and Protein Leakage DOI
Congyang Mao, Yiming Xiang, Xiangmei Liu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2019, Volume and Issue: 11(19), P. 17902 - 17914

Published: April 23, 2019

Bacterial infection is still a ticklish clinical challenge even though some advanced antibacterial materials and techniques have been put forward. This work reports that rapid effective performance achieved by the synergistic local photothermal photodynamic therapy (PTDT). Within 10 min of light irradiation, both Escherichia coli Staphylococcus aureus are almost completely eliminated action photothermy (52.1 °C) limited reactive oxygen species (ROS), corresponding bacterial killing efficiencies 99.91 99.97%, respectively, which far higher than single modal therapy, i.e., or with efficacy 50 70%, respectively. The mechanism membrane permeation increased PTDT because shows more severe impact only on E. destroying outmost panniculus, whereas inner panniculus two kinds bacteria sensitive to ROS. Hence, ROS penetrates easily, meanwhile, proteins in severely lost after disruption, leads death. In vivo results reveal sterilization an important process accelerate wound healing, traumas rats' backbones heal well within 12 days PTDT. Furthermore, friendly major organs rats during therapeutic process. Therefore, system can be safe for great potential. More importantly, presented this has guiding significance design other systems techniques.

Language: Английский

Citations

198
Antimicrobial Peptides and Proteins: From Nature’s Reservoir to the Laboratory and Beyond DOI Creative Commons

Tanumoy Sarkar,

Monikha Chetia,

Sunanda Chatterjee

et al.

Frontiers in Chemistry, Journal Year: 2021, Volume and Issue: 9

Published: June 18, 2021

Rapid rise of antimicrobial resistance against conventional antimicrobials, resurgence multidrug resistant microbes and the slowdown in development new classes necessitates urgent alternate therapeutic molecules. Antimicrobial peptides (AMPs) are small proteins present different lifeforms nature that provide defense microbial infections. They have been effective components host system for a very long time. The fact by AMPs is relatively slower or delayed compared to antibiotics, makes them prospective alternative therapeutics future. Several thousands isolated from various natural sources like microorganisms, plants, insects, crustaceans, animals, humans, etc. date. However, only few translated commercially market so far. This because some inherent drawbacks naturally obtained 1) short half-life owing susceptibility protease degradation, 2) inactivity at physiological salt concentrations, 3) cytotoxicity cells, 4) lack appropriate strategies sustained targeted delivery AMPs. has led surge interest synthetic which would retain improve potency along with circumventing disadvantages analogs. inspired designs sequences strengthened fusion elements. Generation based on sequence truncation, mutation, cyclization introduction unnatural amino acids synthons. In this review, we described vast repertoire sources, subsequently developed templates

Language: Английский

Citations

140
Therapeutic strategies against bacterial biofilms DOI Creative Commons
Yufang Bi,

Guixue Xia,

Chao Shi

et al.

Fundamental Research, Journal Year: 2021, Volume and Issue: 1(2), P. 193 - 212

Published: Feb. 21, 2021

The emergence of multi-drug resistance makes bacterial infection a major threat to public health and economy. formation biofilms is one the main reasons resistance. complexity chemical composition physical structure elimination mature difficult problem. highly antibiotic resistant property urgently calls for potent antimicrobial agents novel antibiofilm strategies. Researchers have made lot efforts in this field. Here we review current strategies eliminate progress related drug delivery nanosystems, with aim inspiring researchers design new systems.

Language: Английский

Citations

135