Acta Biomaterialia,
Journal Year:
2024,
Volume and Issue:
177, P. 107 - 117
Published: Feb. 19, 2024
Designing
proteins
that
fold
and
assemble
over
different
length
scales
provides
a
way
to
tailor
the
mechanical
properties
biological
performance
of
resulting
hydrogels.
In
this
study,
we
designed
modular
self-assemble
into
fibrillar
networks
and,
as
result,
form
hydrogel
materials.
We
incorporated
distinct
functionalities
by
connecting
separate
self-assembling
(A
block)
cell-binding
(B
domains
single
macromolecules.
The
number
affects
rigidity
fibers
final
storage
modulus
G′
hydrogels
could
be
tuned
broad
range
(G′
=
0.1
–
10
kPa),
making
them
suitable
for
cultivation
differentiation
multiple
cell
types,
including
cortical
neurons
human
mesenchymal
stem
cells.
Moreover,
confirmed
bioavailability
attachment
in
can
future
further
tailored
specific
types
or
other
applications.
Finally,
demonstrate
versatility
application
biofabrication
3D
scaffolds
support
growth
guide
their
function.
Designed
enable
decoupling
biophysical
biochemical
within
material
biomaterial
engineering.
context,
present
protein
platform
integrates
blocks)
biopolymer.
linking
assembly
polymer
provided
independent
tuning
inclusion
biofunctional
domains.
use
biofabrication,
neural
culture
printing
differentiation.
Overall,
work
highlights
how
informed
design
biopolymer
sequences
protein-based
with
independently
tunable
properties.
Bioactive Materials,
Journal Year:
2023,
Volume and Issue:
32, P. 98 - 123
Published: Oct. 4, 2023
Tissue
engineering
and
regenerative
medicine
have
shown
potential
in
the
repair
regeneration
of
tissues
organs
via
use
engineered
biomaterials
scaffolds.
However,
current
constructs
face
limitations
replicating
intricate
native
microenvironment
achieving
optimal
capacity
functional
recovery.
To
address
these
challenges,
utilization
decellularized
cell-derived
extracellular
matrix
(ECM)
has
emerged
as
a
promising
approach.
These
biocompatible
bioactive
can
be
into
porous
scaffolds
grafts
that
mimic
structural
compositional
aspects
tissue
or
organ
microenvironment,
both
Engineered Regeneration,
Journal Year:
2023,
Volume and Issue:
5(1), P. 21 - 44
Published: Oct. 27, 2023
Bone
fractures
are
common
occurrence
in
clinical
settings,
creating
a
high
demand
for
effective
repair
material.
Unfortunately,
limited
graft
availability,
donor
site
morbidities,
unpredictable
outcomes,
immunologic
reactions,
infection
risks,
and
geometrical
mismatching
concerns
hampered
tissue
use
underscored
the
need
scaffolds
more
bone
reconstructions
due
to
their
tunable
properties.
Significant
progress
has
been
carried
out
past
decade
fields
of
nanoceramics
synthesis,
bioconjugate
chemistry,
composite
material
processing.
This
review
outlines
hierarchical
structures
biology
tissue,
materialistic
components
(bioceramics,
polymers,
bioactive
drugs),
featured
scaffolding
strategies
(nanofibers,
hydrogels,
aerogels,
bioprinting,
fiber-reinforced
composite),
emphasis
that
physiochemical
characteristics
should
be
used
as
an
inspiration
scaffold
design.
discussed
how
differences
materiobiological
aspects
scaffolds,
such
polymer/bioceramic
nanocomposite,
mineralized
matrix-rich
3D
microenvironmental
cues,
pore
space
mechanical
usage
physical
stimulation
(magnetic,
electroactive,
photoactivated
cues),
surface
cues
(wettability,
roughness,
textured,
charge),
biointerface
(cell–biomaterial
interactions,
cell-selective
homing,
cell
regulatory
strategies)
modulate
cellular
biological
response
engineering.
study
further
challenges
benefits
integrating
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 5, 2024
Abstract
The
biochemical
and
biophysical
properties
of
the
extracellular
matrix
(ECM)
play
a
pivotal
role
in
regulating
cellular
behaviors
such
as
proliferation,
migration,
differentiation.
Engineered
protein‐based
hydrogels,
with
highly
tunable
multifunctional
properties,
have
potential
to
replicate
key
features
native
ECM.
Formed
by
self‐assembly
or
crosslinking,
engineered
hydrogels
can
induce
range
cell
through
bioactive
functional
domains
incorporated
into
polymer
backbone.
Using
recombinant
techniques,
amino
acid
sequence
protein
backbone
be
designed
precise
control
over
chain‐length,
folded
structure,
cell‐interaction
sites.
In
this
review,
modular
design
from
both
molecular‐
network‐level
perspective
are
discussed,
summarize
recent
progress
case
studies
highlight
diverse
strategies
used
construct
biomimetic
scaffolds.
This
review
focuses
on
sequences
that
form
structural
blocks,
stimuli‐responsive
blocks
for
scaffold
properties.
Both
physical
chemical
methods
stabilize
dynamic
networks
defined
structure
bioactivity
culture
applications
discussed.
Finally,
discussion
future
directions
scaffolds
is
concluded.
Biomedicines,
Journal Year:
2024,
Volume and Issue:
12(2), P. 313 - 313
Published: Jan. 29, 2024
The
healing
of
osteochondral
defects
(OCDs)
that
result
from
injury,
osteochondritis,
or
osteoarthritis
and
bear
lesions
in
the
cartilage
bone,
pain,
loss
joint
function
middle-
old-age
individuals
presents
challenges
to
clinical
practitioners
because
non-regenerative
limitations
current
therapies.
Bioactive
peptide-based
(OC)
tissue
regeneration
is
becoming
more
popular
it
does
not
have
immunogenicity,
misfolding,
denaturation
problems
associated
with
original
proteins.
Periodically,
reviews
are
published
on
bone
separately;
however,
none
them
addressed
simultaneous
these
tissues
complicated
heterogeneous
environment
interface.
As
regulators
cell
adhesion,
proliferation,
differentiation,
angiogenesis,
immunomodulation,
antibacterial
activity,
potential
therapeutic
strategies
for
OCDs
utilizing
cartilage-specific
peptides
should
be
examined
investigated.
main
goal
this
review
was
study
how
they
contribute
OCDs,
either
alone
conjunction
other
biomaterials.
Chemical Society Reviews,
Journal Year:
2024,
Volume and Issue:
53(8), P. 3714 - 3773
Published: Jan. 1, 2024
Self-assembled
monolayers
(SAMs)
based
on
peptides
represent
a
reproducible
platform
for
screening
and
studying
peptide
functions
facilitating
their
translation
into
multidisciplinary
applications.
Langmuir,
Journal Year:
2023,
Volume and Issue:
39(34), P. 11935 - 11945
Published: Aug. 17, 2023
Peptides
are
versatile
building
blocks
for
the
fabrication
of
various
nanostructures
that
result
in
formation
hydrogels
and
nanoparticles.
Precise
chemical
functionalization
promotes
discrete
structure
formation,
causing
controlled
bioactivity
physical
properties
functional
materials
development.
The
conjugation
small
molecules
on
amino
acid
side
chains
determines
their
intermolecular
interactions
addition
to
intrinsic
peptide
characteristics.
Molecular
information
affects
structure,
activity.
In
this
Perspective,
blocks,
nanostructure
mechanisms,
these
discussed
with
results
recent
publications.
Bioinstructive
stimuli-responsive
have
immense
impacts
nanomedicine
field
including
drug
delivery,
cellular
engineering,
regenerative
medicine,
biomedicine.
