Gathering brings strength: How organic aggregates boost disease phototheranostics

Aggregate, Journal Year: 2021, Volume and Issue: 2(1), P. 95 - 113

Published: Jan. 22, 2021

Abstract Phototheranostics that concurrently and complementarily integrate real‐time diagnosis in situ therapeutic capabilities one platform has become the advancing edge of precision medicine. Organic agents possess merits facile preparation, high purity, tunable photophysical property, good biocompatibility, potential biodegradability, which have shown great promise for disease theranostics. This review summarizes recent achievements organic phototheranostic applications, especially rationally utilize energy dissipation pathways Jablonski diagram to modulate fluorescence emission, photoacoustic/photothermal production, photodynamic processes. Of particular interest are systems exhibiting huge differences aggregate state as compared with solution or single molecule form, during intramolecular motions play an important role regulating properties. The advances from such aspect biomedical applications including high‐resolution imaging, activatable imaging therapy, adaptive theranostics, image‐guided surgery, immunotherapy, afterglow discussed. A brief summary perspective this field also presented. We hope will be helpful researchers interested bioprobe design theranostic inspire new insights into linkage between science field.

Language: Английский

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Stimuli-responsive materials: A smart way to study dynamic cell responses

Smart Materials in Medicine, Journal Year: 2022, Volume and Issue: 3, P. 257 - 273

Published: Jan. 1, 2022

Cells in the body reside within extracellular matrix (ECM), a three-dimensional environment that not only provides structural support for cells, but also influences cellular processes, like migration and differentiation. The ECM cells continuously engage complex highly dynamic interplay, shaping both as well outcome. To study these dynamic, bidirectional interactions systematic manner, ability to dynamically control environments is desirable. Stimuli-responsive materials are class of have been engineered respond external cues, e.g., light, electricity, or magnetic field, therefore hold fascinating potentials an ideal experimental platform introduce changing spatiotemporal signals cells. Here, we review state art stimuli-responsive their design strategies, with emphasis on introduction physical mechanical cues. effects such stimuli responses living examined three different levels: phenotypes, intracellular cytoskeletal changes, nuclear epigenetic effects. Finally, discuss current challenges limitations potential outlooks exploiting biomaterials.

Language: Английский

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Static and Dynamic: Evolving Biomaterial Mechanical Properties to Control Cellular Mechanotransduction

Advanced Science, Journal Year: 2023, Volume and Issue: 10(9)

Published: Jan. 19, 2023

The extracellular matrix (ECM) is a highly dynamic system that constantly offers physical, biological, and chemical signals to embraced cells. Increasing evidence suggests mechanical derived from the cellular microenvironment are essential controllers of cell behaviors. Conventional culture biomaterials, with static properties such as chemistry, topography, stiffness, have offered fundamental understanding various vital biochemical biophysical processes, adhesion, spreading, migration, growth, differentiation. At present, novel biomaterials can spatiotemporally impart cues manipulate fate emerging. adaptive traits new materials endow them ability adapt requirements enhance functions. In this review, an introductory overview key players mechanobiology provided. A perspective on state-of-the-art manipulation techniques in designing ECM-mimicking taken. particular, different regulating mechanosensing functions compared. This review benefit development engineering biomechanical systems expected.

Language: Английский

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Polysaccharides, proteins, and synthetic polymers based multimodal hydrogels for various biomedical applications: A review

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 247, P. 125606 - 125606

Published: July 3, 2023

Language: Английский

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Dynamic Stimulations with Bioengineered Extracellular Matrix‐Mimicking Hydrogels for Mechano Cell Reprogramming and Therapy

Advanced Science, Journal Year: 2023, Volume and Issue: 10(21)

Published: April 29, 2023

Abstract Cells interact with their surrounding environment through a combination of static and dynamic mechanical signals that vary over stimulus types, intensity, space, time. Compared to such as stiffness, porosity, topography, the current understanding on effects stimulations cells remains limited, attributing lack access devices, complexity experimental set‐up, data interpretation. Yet, in pursuit emerging translational applications (e.g., cell manufacturing for clinical treatment), it is crucial understand how respond variety forces are omnipresent vivo so they can be exploited enhance therapeutic outcomes. With rising appreciation extracellular matrix (ECM) key regulator biofunctions, researchers have bioengineered suite ECM‐mimicking hydrogels, which fine‐tuned spatiotemporal cues model complex profiles. This review first discusses stimuli may impact different cellular components various mechanobiology pathways involved. Then, hydrogels designed incorporate parameters influence behaviors described. The Scopus database also used analyze relative strength evidence, ranging from strong weak, based number published literatures, associated citations, treatment significance. Additionally, impacts clinically relevant types including mesenchymal stem cells, fibroblasts, immune evaluated. aim draw attention paucity studies well highlight potential using cocktail intensities fates (similar concept biochemical direct fate). It envisioned this progress report will inspire more exciting development mechanoresponsive biomedical applications.

Language: Английский

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Extracellular Matrix Cues Regulate Mechanosensing and Mechanotransduction of Cancer Cells

Cells, Journal Year: 2024, Volume and Issue: 13(1), P. 96 - 96

Published: Jan. 2, 2024

Extracellular biophysical properties have particular implications for a wide spectrum of cellular behaviors and functions, including growth, motility, differentiation, apoptosis, gene expression, cell–matrix cell–cell adhesion, signal transduction mechanotransduction. Cells not only react to unambiguously mechanical cues from the extracellular matrix (ECM), but can occasionally manipulate features in parallel with biological characteristics, thus interfering downstream matrix-based both physiological pathological processes. Bidirectional interactions between cells (bio)materials vitro alter cell phenotype mechanotransduction, as well ECM structure, intentionally or unintentionally. Interactions mechanics vivo are importance variety diseases, primarily cancer. Stiffness values normal cancerous tissue range 500 Pa (soft) 48 kPa (stiff), respectively. Even shear flow increase 0.1–1 dyn/cm2 (normal tissue) 1–10 (cancerous tissue). There currently many new areas activity tumor research on various length scales, which highlighted this review. Moreover, complexity cancer is reduced common different tumors characteristics identify main pathways interaction. This all contributes standardization mechanotransduction models approaches, which, ultimately, increases understanding complex Finally, effects mechanics–biology pairing key insights clinical practice treatment and, consequently, translation.

Language: Английский

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Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

Small, Journal Year: 2019, Volume and Issue: 16(9)

Published: Oct. 11, 2019

Fabrication of artificial biomimetic materials has attracted abundant attention. As one the subcategories materials, cells are highly significant for multiple disciplines and their synthesis been intensively pursued. In order to manufacture robust "alive" with high throughput, easy operation, precise control, flexible microfluidic techniques widely utilized. Herein, recent advances in microfluidic-based methods droplets, vesicles, summarized. First, droplet fabrication manipulation on T-junction, flow-focusing, coflowing devices discussed. Then, formation unicompartmental multicompartmental vesicles based microfluidics Furthermore, engineering droplet-based vesicle-based by is also reviewed. Moreover, applied imitating cell behavior acting as bioreactors synthetic biology highlighted. Finally, current challenges future trends This review should be helpful researchers fields microfluidics, biomaterial fabrication, biology.

Language: Английский

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Biomimetic micro/nano structures for biomedical applications

Nano Today, Journal Year: 2020, Volume and Issue: 35, P. 100980 - 100980

Published: Sept. 28, 2020

Language: Английский

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Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel

Heliyon, Journal Year: 2019, Volume and Issue: 5(4), P. e01474 - e01474

Published: April 1, 2019

Several hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform mimic native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility soft texture. However, more extensive biocompatibility interactions studies with cell lines needed. Therefore, aim this study is focus on evaluating PNIPAM through cytotoxicity, genotoxicity, proliferation tests murine preadipose cells (3T3-L1), human embryonic kidney (HEK293) carcinoma-derived (A549) presence surfaces. Bioadhesive capacity above was also analyzed. MTT neutral red uptake assays shown non-cytotoxic effect studied lines. Genotoxicity evaluated by single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed corroborate had progressed correctly. Adopted morphologies for each line over similar growing observed polystyrene, indicating favor attachment during 5 days' culture. The good make it an interesting scaffold clinical potential tissue regeneration engineering, possible adipose tissue-engineered construct.

Language: Английский

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Lanthanide-Doped Upconversion Nanoparticles Meet the Needs for Cutting-Edge Bioapplications: Recent Progress and Perspectives

ACS Materials Letters, Journal Year: 2020, Volume and Issue: 2(11), P. 1516 - 1531

Published: Oct. 16, 2020

The rise of biological frontier fields such as precision theranostics, gene editing, optogenetics, etc. introduces the best opportunities and unprecedented challenges at same time to bioapplication luminescent nanomaterials. Because merits photon upconversion characteristics high stability, tunable structure excitation dynamics, sharp emissions, lanthanide-doped nanoparticle (UCNP) is considered be a highly competitive candidate meet these challenges. Indeed, UCNPs have attracted extensive attention in diverse cutting-edge ranging from near-zero background biosensing, deep tissue bioimaging, nanomedicine, remote biomanipulation since first development early 2000s. Recently, with increasing maturity synthesis technology integration multiple disciplines, application research has achieved many new breakthroughs. Herein, we summarize latest progress sensing, imaging, therapy, Ultimately, current perspectives this field are discussed.

Language: Английский

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