Spray-Induced Gene Silencing (SIGS): Nanocarrier-Mediated dsRNA Delivery Improves RNAi Efficiency in the Management of Lettuce Gray Mold Caused by Botrytis cinerea DOI Creative Commons
Maria Spada, Claudio Pugliesi, Marco Fambrini

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(1), P. 194 - 194

Published: Jan. 15, 2025

The plant pathogenic fungus Botrytis cinerea causes significant losses in agricultural production and it is rather difficult to control due its broad host range environmental persistence. management of gray mold disease still mainly based on the use chemicals, which could have harmful effects not only impacts environment human health, but also because they favor development fungicide-resistant strains. In this scenario, strategy RNA interference (RNAi) being widely considered, Spray-Induced Gene Silencing (SIGS) gaining interest as a versatile, sustainable, effective, environmentally friendly alternative chemicals protection crops. SIGS approach was evaluated B. infection lettuce plants. vitro-synthesized dsRNA molecules (BcBmp1-, BcBmp3-, BcPls1-dsRNAs) were used naked, or complexed small layered double hydroxide (sLDH) clay nanosheets. Therefore, treatments applied by pressure spraying whole plants lately inoculated with cinerea. All sprayed dsRNAs proved effective reducing symptoms notable reduction compared controls. effectiveness caused high overall increased significantly sLDH nanosheet–dsRNA complexes showed better over time naked particularly evident at 27 days post-inoculation. RNAi-based biocontrol be an excellent chemical fungicides, several products are expected approved soon, although will face challenges before reaching market.

Language: Английский

Fungal small RNAs ride in extracellular vesicles to enter plant cells through clathrin-mediated endocytosis DOI Creative Commons
Baoye He, Huan Wang, Guosheng Liu

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 20, 2023

Small RNAs (sRNAs) of the fungal pathogen Botrytis cinerea can enter plant cells and hijack host Argonaute protein 1 (AGO1) to silence immunity genes. However, mechanism by which these sRNAs are secreted remains unclear. Here, we demonstrate that B. utilizes extracellular vesicles (EVs) secrete Bc-sRNAs, then internalized through clathrin-mediated endocytosis (CME). The tetraspanin protein, Punchless (BcPLS1), serves as an EV biomarker plays essential role in pathogenicity. We observe numerous Arabidopsis clathrin-coated (CCVs) around infection sites colocalization marker BcPLS1 CLATHRIN LIGHT CHAIN 1, one core components CCV. Meanwhile, cinerea-secreted detected purified CCVs after infection. knockout mutants inducible dominant-negative key CME pathway exhibit increased resistance Furthermore, Bc-sRNA loading into AGO1 target gene suppression attenuated those mutants. Together, our results fungi via EVs, mainly CME.

Language: Английский

Citations

59
Can biocontrol be the game-changer in integrated pest management? A review of definitions, methods and strategies DOI Creative Commons
Matteo Galli, F. Feldmann,

Ute Vogler

et al.

Journal of Plant Diseases and Protection, Journal Year: 2024, Volume and Issue: 131(2), P. 265 - 291

Published: Feb. 23, 2024

Abstract Global agriculture is heavily dependent on sustainable plant protection. Worldwide, the concept of integrated pest management (IPM) being followed. IPM utilizes a range strategies, with chemical synthetic pesticides employed only as last resort. However, in agricultural practice, farmers continue to rely primarily this option. To further reduce dependence, new strategies are sought strengthen use biological control within approach including identification novel non-synthetic natural compounds. Here, we discuss and report state art research areas such biocontrol agents application ecological principles. These practices can help establish protection systems, greatest impact achieved when they used appropriate combinations. We highlight conditions that currently prevent or hinder increased measures. On background agroecological experiences, why additional advancements imperative more effectively break life cycles pests, diseases weeds. emphasize significance judicious technologies, adapted local conditions. Additionally, key role expertise operators implementing these their knowledge thereof.

Language: Английский

Citations

38
Risk assessment of RNAi-based biopesticides DOI Creative Commons

Xuming Luo,

Satyabrata Nanda, Youjun Zhang

et al.

New Crops, Journal Year: 2024, Volume and Issue: 1, P. 100019 - 100019

Published: March 18, 2024

RNA interference (RNAi) is an efficient molecular approach in which double-stranded (dsRNA) can effectively knock down the expression of certain genes, promoting mRNA degradation, and gene function loss. Based on principle RNAi, a novel eco-friendly biopesticide has been characterized used recent years. RNAi-based biopesticides have high specificity, efficiency, easy ushered third revolution history pesticides. Additionally, these properties are great significance achieving sustainable agricultural development. However, before market release any biopesticides, it essential to consider whether they will adversely affect human health environment. This paper reviews biopesticides' different modes action diverse associated risks, such as environmental risk (environmental fate, off-target effects, exposure assessment, resistance development), risk, government policies.

Language: Английский

Citations

17
Fungal impacts on Earth’s ecosystems DOI
Nicola T. Case, Sarah J. Gurr, Matthew C. Fisher

et al.

Nature, Journal Year: 2025, Volume and Issue: 638(8049), P. 49 - 57

Published: Feb. 5, 2025

Language: Английский

Citations

5
Crop protection by RNA interference: a review of recent approaches, current state of developments and use as of 2013 DOI Creative Commons
Kirsten Germing, Camila Navarrete,

Andreas Schiermeyer

et al.

Environmental Sciences Europe, Journal Year: 2025, Volume and Issue: 37(1)

Published: Jan. 25, 2025

Language: Английский

Citations

2
Exogenous dsRNA triggers sequence-specific RNAi and fungal stress responses to control Magnaporthe oryzae in Brachypodium distachyon DOI Creative Commons
Ying Zheng, Benjamin Moorlach, Désirée Jakobs‐Schönwandt

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 25, 2025

In vertebrates and plants, dsRNA plays crucial roles as PAMP a mediator of RNAi. How higher fungi respond to is not known. We demonstrate that Magnaporthe oryzae (Mo), globally significant crop pathogen, internalizes across broad size range 21 about 3000 bp. Incubation fungal conidia with 10 ng/µL dsRNA, regardless or sequence, induced aberrant germ tube elongation, revealing strong sequence-unspecific effect in this fungus. Accordingly, the synthetic analogue poly(I:C) various sizes sequences elicited canonical stress pathways, including nuclear accumulation marker mitogen-activated protein kinase Hog1p production ROS. Leaf application cereal model species Brachypodium distachyon suppressed progression leaf blast disease. Notably, depends on doses, while pure sequence-specific effects were observed at low concentrations ( < 0.03 ng/µL). The protective further enhanced by maintaining gap least seven days between inoculation, stabilising alginate-chitosan nanoparticles. Overall, our study opens up additional possibilities for development use pesticides agriculture.

Language: Английский

Citations

2
Extracellular vesicles: cross-organismal RNA trafficking in plants, microbes, and mammalian cells DOI Open Access

Qiang Cai,

Lida Halilovic,

Ting Shi

et al.

Extracellular Vesicles and Circulating Nucleic Acids, Journal Year: 2023, Volume and Issue: 4(2), P. 262 - 282

Published: Jan. 1, 2023

Extracellular vesicles (EVs) are membrane-enclosed nanometer-scale particles that transport biological materials such as RNAs, proteins, and metabolites. EVs have been discovered in nearly all kingdoms of life a form cellular communication across different cells between interacting organisms. EV research has primarily focused on EV-mediated intra-organismal mammals, which led to the characterization plethora contents from diverse cell types with distinct impactful physiological effects. In contrast, into plants inter-organismal interactions microbes. However, overall molecular content functions plant microbial remain largely unknown. Recent studies plant-pathogen interface demonstrated produce secrete small RNAs pathogen silence virulence-related genes. Plant-interacting microbes bacteria fungi also metabolites, potentially enhance their virulence. This review will focus recent advances communications compared current state knowledge mammalian capabilities highlight role cross-kingdom RNA interference.

Language: Английский

Citations

32
Improving RNA-based crop protection through nanotechnology and insights from cross-kingdom RNA trafficking DOI
Angela Chen,

Lida Halilovic,

Jia-Hong Shay

et al.

Current Opinion in Plant Biology, Journal Year: 2023, Volume and Issue: 76, P. 102441 - 102441

Published: Sept. 9, 2023

Language: Английский

Citations

29
Recent Advances in Nanoparticle-Mediated Co-Delivery System: A Promising Strategy in Medical and Agricultural Field DOI Open Access
Mingshan Li, Xiaowei Sun, Meizhen Yin

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5121 - 5121

Published: March 7, 2023

Drug and gene delivery systems mediated by nanoparticles have been widely studied for life science in the past decade. The application of nano-delivery can dramatically improve stability efficiency carried ingredients, overcoming defects administration routes cancer therapy, possibly maintaining sustainability agricultural systems. However, a drug or alone sometimes cannot achieve satisfactory effect. nanoparticle-mediated co-delivery system load multiple drugs genes simultaneously, effectiveness each component, thus amplifying efficacy exhibiting synergistic effects therapy pest management. has reported medical field, studies on its field recently begun to emerge. In this progress report, we summarize recent preparation discuss remaining challenges future perspectives design fabrication.

Language: Английский

Citations

28
The role of polymers in enabling RNAi-based technology for sustainable pest management DOI Creative Commons
Ana Isabel Quilez‐Molina, Jonatan Niño‐Sánchez, Danila Merino

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 23, 2024

The growing global food demand, coupled with the limitations of traditional pest control methods, has driven search for innovative and sustainable solutions in agricultural management. In this review, we highlight polymeric nanocarriers their potential to deliver double-stranded RNA (dsRNA) pests through gene-silencing mechanism interference (RNAi). Polymer-dsRNA systems have shown promise protecting dsRNA, facilitating cellular uptake, ensuring precise release. Despite these advances, challenges such as scalability, cost-efficiency, regulatory approval, public acceptance persist, necessitating further research overcome obstacles fully unlock RNAi agriculture.

Language: Английский

Citations

12