Journal of Aging and Physical Activity,
Journal Year:
2024,
Volume and Issue:
32(4), P. 531 - 540
Published: April 29, 2024
Resistance
training
is
used
to
combat
skeletal
muscle
function
decline
in
older
adults.
Few
studies
have
been
designed
specific
for
females,
resulting
very
limited
treatment
options
atrophy
aging
women.
Here,
we
analyzed
the
gene
expression
profiles
of
samples
from
sedentary
young
women,
and
resistance-trained
using
microarray
data
public
database.
A
total
45
genes
that
were
differentially
expressed
during
female
reversed
by
resistance
identified.
Functional
pathway
enrichment
analysis,
protein-protein
interaction
network
receiver
operating
characteristic
analysis
performed
reveal
key
pathways
involved
effects
on
aging.
The
collagen
COL1A1,
COL3A1,
COL4A1
identified
important
regulators
training,
modulating
multiple
signaling
pathways,
such
as
PI3
kinase-Akt
signaling,
focal
adhesions,
extracellular
matrix-receptor
interactions,
relaxin
signaling.
Interestingly,
CDKN1A
TP63
increased
aging,
further
upregulated
suggesting
they
may
negatively
affect
outcomes.
Our
findings
provide
novel
insights
into
molecular
mechanisms
identify
potential
biomarkers
targets
clinical
intervention.
Physiological Reviews,
Journal Year:
2023,
Volume and Issue:
103(4), P. 2679 - 2757
Published: June 29, 2023
Mechanisms
underlying
mechanical
overload-induced
skeletal
muscle
hypertrophy
have
been
extensively
researched
since
the
landmark
report
by
Morpurgo
(1897)
of
“work-induced
hypertrophy”
in
dogs
that
were
treadmill
trained.
Much
preclinical
rodent
and
human
resistance
training
research
to
date
supports
involved
mechanisms
include
enhanced
mammalian/mechanistic
target
rapamycin
complex
1
(mTORC1)
signaling,
an
expansion
translational
capacity
through
ribosome
biogenesis,
increased
satellite
cell
abundance
myonuclear
accretion,
postexercise
elevations
protein
synthesis
rates.
However,
several
lines
past
emerging
evidence
suggest
additional
feed
into
or
are
independent
these
processes
also
involved.
This
review
first
provides
a
historical
account
how
mechanistic
has
progressed.
A
comprehensive
list
associated
with
is
then
outlined,
areas
disagreement
involving
presented.
Finally,
future
directions
many
discussed
proposed.
AJP Cell Physiology,
Journal Year:
2023,
Volume and Issue:
324(6), P. C1274 - C1294
Published: May 8, 2023
Skeletal
muscle
memory
is
an
exciting
phenomenon
gaining
significant
traction
across
several
scientific
communities,
among
exercise
practitioners,
and
the
public.
Research
has
demonstrated
that
skeletal
tissue
can
be
“primed”
by
earlier
positive
encounters
with
training
enhance
adaptation
to
later
retraining,
even
following
periods
of
cessation
or
detraining.
This
review
will
describe
discuss
most
recent
research
investigating
underlying
mechanisms
memory:
1)
“cellular”
and,
2)
“epigenetic”
memory,
as
well
emerging
evidence
how
these
theories
may
work
in
synergy.
We
both
“positive”
“negative”
highlight
importance
for
optimizing
interventions
programs
development
therapeutic
strategies
counteracting
wasting
conditions
age-related
loss.
Finally,
important
directions
field
highlighted
advance
next
generation
studies
into
future.
Aging Cell,
Journal Year:
2024,
Volume and Issue:
23(4)
Published: Feb. 21, 2024
Evaluation
of
the
influence
primary
and
secondary
aging
on
manifestation
molecular
cellular
hallmarks
is
a
challenging
currently
unresolved
issue.
Our
study
represents
first
demonstration
distinct
role
chronic
inflammation/physical
inactivity
-
most
important
drivers
aging,
in
regulation
transcriptomic
proteomic
profiles
human
skeletal
muscle.
To
achieve
this
purpose,
young
healthy
people
(n
=
15),
8)
older
37)
patients
with
knee/hip
osteoarthritis,
model
to
effect
long-term
inflammation
vastus
lateralis
muscle,
were
included
study.
It
was
revealed
that
widespread
substantial
age-related
changes
gene
expression
relative
(~4000
genes
regulating
mitochondrial
function,
proteostasis,
cell
membrane,
secretory
immune
response)
related
physical
rather
than
aging.
Primary
contributed
mainly
(~200)
encoding
nuclear
proteins
(regulators
DNA
repair,
RNA
processing,
transcription),
(genes
respiratory
enzymes,
complex
assembly
factors,
regulators
cristae
formation
reactive
oxygen
species
production),
as
well
proteostasis.
found
associated
regulated
at
post-transcriptional
level.
The
set
putative
their
potential
transcriptional
can
be
used
resource
for
further
targeted
studies
investigating
individual
transcription
factors
emergence
senescent
phenotype.
The Journal of Physiology,
Journal Year:
2024,
Volume and Issue:
603(1), P. 211 - 237
Published: July 26, 2024
Exercise
is
a
potent
stimulus
for
combatting
skeletal
muscle
ageing.
To
study
the
effects
of
exercise
on
in
preclinical
setting,
we
developed
combined
endurance-resistance
training
mice
called
progressive
weighted
wheel
running
(PoWeR).
PoWeR
improves
molecular,
biochemical,
cellular
and
functional
characteristics
promotes
aspects
partial
epigenetic
reprogramming
when
performed
late
life
(22-24
months
age).
In
this
investigation,
leveraged
pan-mammalian
DNA
methylome
arrays
tandem
mass-spectrometry
proteomics
to
provide
detailed
information
late-life
adaptations
female
relative
age-matched
sedentary
controls
(n
=
7-10
per
group).
Differential
CpG
methylation
at
conserved
promoter
sites
was
related
transcriptional
regulation
genes
as
well
Nr4a3,
Hes1
Hox
after
PoWeR.
Using
holistic
method
-omics
integration
binding
expression
target
analysis
(BETA),
changes
were
associated
with
upregulated
proteins
global
mitochondrial
translation
(P
0.03).
Specifically,
BETA
implicated
control
ribosomal,
mitoribosomal,
complex
I
protein
abundance
training.
may
also
influence
LACTB,
MIB1
UBR4
induction
-
all
are
mechanistically
linked
health.
Computational
cistrome
predicted
several
transcription
factors
including
MYC
regulators
trained
methylome-proteome
landscape,
corroborating
prior
transcriptome
data.
Correlating
proteome
mass
fatigue
resistance
revealed
positive
relationships
VPS13A
NPL
levels,
respectively.
Our
findings
expose
differential
proteomic
translational
that
could
function
aged
mice.
KEY
POINTS:
Late-life
from
22-24
age
shown
improve
vivo
promote
mitigation.
Integration
36k
using
(which
contain
ageing
clock
sites)
exploratory
extends
our
work
reveals
coordinated
widespread
initiation,
ribosomal
(mitoribosomal)
voluntary
sizeable
cohort
group
analysis).
Multi-omics
serine
β-lactamase-like
(LACTB
tumour
muscle),
mind
bomb
1
(MIB1
satellite
cell
type
2
fibre
maintenance)
ubiquitin
ligase
E3
component
N-recognin
4
(UBR4
quality
control)
identified
regulator
proteome,
agreement
analyses.
Vacuolar
sorting
13
homolog
A
(VPS13A)
positively
correlated
mass,
glycoprotein/glycolipid
sialylation
enzyme
N-acetylneuraminate
pyruvate
lyase
(NPL)
resistance.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: March 27, 2024
Abstract
Molecular
control
of
recovery
after
exercise
in
muscle
is
temporally
dynamic.
A
time
course
biopsies
around
resistance
(RE)
combined
with
-omics
necessary
to
better
comprehend
the
molecular
contributions
skeletal
adaptation
humans.
Vastus
lateralis
before
and
30
minutes,
3-,
8-,
24-hours
acute
RE
were
collected.
time-point
matched
biopsy-only
group
was
also
included.
RNA-sequencing
defined
transcriptome
while
DNA
methylomics
computational
approaches
complemented
these
data.
The
post-RE
revealed:
1)
methylome
responses
at
minutes
corresponded
upregulated
genes
3
hours,
2)
a
burst
translation-
transcription-initiation
factor-coding
transcripts
occurred
between
8
3)
global
gene
expression
peaked
4)
ribosome-related
dominated
mRNA
landscape
24
5)
methylation-regulated
MYC
highly
influential
transcription
factor
throughout
24-hour
played
primary
role
levels
hours.
influence
human
strengthened
by
information
from
overexpression
mouse
muscle.
To
test
whether
sufficient
for
hypertrophy,
we
generated
fiber-specific
doxycycline
inducible
model
pulsatile
induction.
Periodic
48-hour
pulses
over
4
weeks
resulted
higher
mass
fiber
size
soleus
adult
female
mice.
Collectively,
present
resolved
resource
understanding
adaptations
reveal
as
regulator
RE-induced
hypertrophy.
Aging and Disease,
Journal Year:
2024,
Volume and Issue:
unknown, P. 0 - 0
Published: Jan. 1, 2024
The
proportion
of
the
elderly
population
is
gradually
increasing
as
a
result
medical
care
advances,
leading
to
subsequent
surge
in
geriatric
diseases
that
significantly
impact
quality
life
and
pose
substantial
healthcare
burden.
Sarcopenia,
characterized
by
age-related
decline
skeletal
muscle
mass
quality,
affects
considerable
portion
older
adults,
particularly
elderly,
can
adverse
outcomes
such
frailty,
fractures,
bedridden,
hospitalization,
even
mortality.
Skeletal
aging
accompanied
underlying
metabolic
changes.
Therefore,
elucidating
these
profiles
specific
mechanisms
holds
promise
for
informing
prevention
treatment
strategies
sarcopenia.
This
review
provides
comprehensive
overview
key
metabolites
identified
current
clinical
studies
on
sarcopenia
their
potential
pathophysiological
alterations
activity.
Besides,
we
examine
therapeutic
from
perspective
focused
regulation.
Cell Metabolism,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 1, 2025
Plasticity
of
skeletal
muscle
is
induced
by
transcriptional
and
translational
events
in
response
to
exercise,
leading
multiple
health
performance
benefits.
The
microenvironment
harbors
myofibers
mononuclear
cells,
but
the
rich
cell
diversity
has
been
largely
ignored
relation
exercise
adaptations.
Using
our
workflow
transcriptome
profiling
individual
myofibers,
we
observed
that
their
exercise-induced
was
surprisingly
modest
compared
with
bulk
tissue
response.
Through
integration
single-cell
data,
identified
a
small
mast
population
likely
responsible
for
histamine
secretion
during
targeting
myeloid
vascular
cells
rather
than
myofibers.
We
demonstrated
through
H1
or
H2
receptor
blockade
humans
this
paracrine
signaling
cascade
drives
glycogen
resynthesis
coordinates
Altogether,
cellular
deconstruction
human
uncovers
histamine-driven
intercellular
communication
network
steering
recovery
adaptation
exercise.
Journal of sport and health science/Journal of Sport and Health Science,
Journal Year:
2025,
Volume and Issue:
unknown, P. 101029 - 101029
Published: Feb. 1, 2025
Advances
in
skeletal
muscle
omics
has
expanded
our
understanding
of
exercise-induced
adaptations
at
the
molecular
level.
Over
past
2
decades,
transcriptome
studies
have
detailed
acute
and
chronic
responses
to
resistance,
endurance,
concurrent
exercise,
focusing
on
variables
such
as
training
status,
nutrition,
age,
sex,
metabolic
health
profile.
Multi-omics
approaches,
integration
transcriptomic
epigenetic
data,
along
with
emerging
ribosomal
RNA
sequencing
advancements,
further
provided
insights
into
how
adapts
exercise
across
lifespan.
Downstream
transcriptome,
proteomic
phosphoproteomic
identified
novel
regulators
adaptations,
while
single-cell/nucleus
spatial
technologies
promise
evolve
cellular
specialization
communication
around
cells.
This
narrative
review
highlights
(a)
historical
foundations
muscle,
(b)
current
research
3
layers
cascade
(DNA,
RNA,
protein),
(c)
applications
single-cell
study
adaptation
exercise.
Further
elaboration
muscle's
global
footprint
using
multi-omics
methods
will
help
researchers
practitioners
develop
more
effective
targeted
approaches
improve
well
athletic
performance.
