Magnetic Control of Multiscale Ligand Nanoarchitecture Regulates Stem Cell Fate DOI Open Access
Ramar Thangam, Hyunsik Hong, Nayeon Kang

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Abstract Native tissues exhibit hierarchical structures of anisotropically arranged extracellular matrix that dynamically regulate stem cells and tissue function. However, neither multiscale nano‐anisotropy nor dynamic anisotropy control have been reported. In this study, spherical or rod‐shaped gold small‐nanomaterials (at integrin receptor‐scale; tens nanometers) are coupled to the surface magnetic large‐nanomaterials focal adhesion complex‐scale; hundreds nanometers), with both showing constant areas at each respective scale. Each nanocomposite is flexibly conjugated substrate material densities, resulting in dual‐scale liganded nano‐anisotropies. Increasing aspect ratio nanomaterials nanometer‐scale dominantly promotes recruitment, adhesion, mechanotransduction, differentiation over nanometer‐scale. Such scale‐specific effects on cell regulation temporally regulated vitro vivo by physically raising lowering nanocomposites respectively inhibit stimulate curved surfaces modulating membrane bending. unprecedented “dynamic ligand anisotropy” can be independently engineered regarding scales, anisotropies, ligands elucidate cell‐material interactions allow for multimodal enhance tissue‐regenerative therapy.

Language: Английский

Advances in Spheroids and Organoids on a Chip DOI Creative Commons
Guocheng Fang, Yu‐Cheng Chen, Hongxu Lu

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(19)

Published: Feb. 22, 2023

Abstract Multicellular spheroids and organoids are promising in vitro 3D models personalized medicine drug screening. They replicate the structural functional characteristics of human organs vivo. Microfluidic technology micro‐nano fabrication can fulfill high requirement engineering approach growing research interest organoids. In this review, comparatively introduced. Then it is illustrated how spheroids‐ organoids‐on‐a‐chip facilitates their establishment, expansion, application through spatial‐temporal control, mechanical cues modeling, high‐throughput analysis, co‐culture, multi‐tissue interactions, biosensing, bioimaging integration. The potential opportunities challenges developing finally outlooked.

Language: Английский

Citations

75
Breaking the resolution limits of 3D bioprinting: future opportunities and present challenges DOI Creative Commons
Tommaso Zandrini,

Sammy Florczak,

Riccardo Levato

et al.

Trends in biotechnology, Journal Year: 2022, Volume and Issue: 41(5), P. 604 - 614

Published: Dec. 10, 2022

Bioprinting aims to produce 3D structures from which embedded cells can receive mechanical and chemical stimuli that influence their behavior, direct organization migration, promote differentiation, in a similar way what happens within the native extracellular matrix. However, limited spatial resolution has been bottleneck for conventional bioprinting approaches. Reproducing fine features at cellular scale, while maintaining reasonable printing volume, is necessary enable biofabrication of more complex functional tissue organ models. In this opinion article we recount emergence of, discuss most promising, high-definition (HD) techniques achieve goal, discussing obstacles remain be overcome, applications are envisioned engineering field.

Language: Английский

Citations

74
Photoinduced Dithiolane Crosslinking for Multiresponsive Dynamic Hydrogels DOI Creative Commons
Benjamin R. Nelson, Bruce E. Kirkpatrick, Connor E. Miksch

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 30, 2023

While many hydrogels are elastic networks crosslinked by covalent bonds, viscoelastic with adaptable crosslinks increasingly being developed to better recapitulate time and position-dependent processes found in tissues. In this work, 1,2-dithiolanes presented as dynamic photocrosslinkers of hydrogels, resulting disulfide bonds throughout the hydrogel that respond multiple stimuli. Using lipoic acid a model dithiolane, formed under physiological conditions, enabling cell encapsulation via an initiator-free light-induced dithiolane ring-opening photopolymerization. The allow for photoinduced responses including stress relaxation, stiffening, softening, network functionalization using single chemistry, which can be supplemented permanent reaction alkenes further control properties connectivity irreversible thioether crosslinks. Moreover, complementary photochemical approaches used achieve rapid complete sample degradation radical scission post-gelation stiffening when irradiated presence reactive gel precursor. results herein demonstrate versatility material chemistry study direct 2D 3D cell-material interactions. This work highlights dithiolane-based photocrosslinking robust method generating range biologically relevant mechanical chemical varied on demand.

Language: Английский

Citations

56
Cancer organoids 2.0: modelling the complexity of the tumour immune microenvironment DOI
Roel Polak, Elisa Zhang, Calvin J. Kuo

et al.

Nature reviews. Cancer, Journal Year: 2024, Volume and Issue: 24(8), P. 523 - 539

Published: July 8, 2024

Language: Английский

Citations

54
In situ modulation of intestinal organoid epithelial curvature through photoinduced viscoelasticity directs crypt morphogenesis DOI Creative Commons
F. Max Yavitt, Bruce E. Kirkpatrick, Michael R. Blatchley

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(3)

Published: Jan. 20, 2023

Spatiotemporally coordinated transformations in epithelial curvature are necessary to generate crypt-villus structures during intestinal development. However, the temporal regulation of mechanotransduction pathways that drive crypt morphogenesis remains understudied. Intestinal organoids have proven useful study vitro, yet reliance on static culture scaffolds limits ability assess effects changing curvature. Here, a photoinduced hydrogel cross-link exchange reaction is used spatiotemporally alter and how dynamic changes influence instruct morphogenesis. Photopatterned increased membrane tension depolarization, which was required for subsequent nuclear localization yes-associated protein 1 (YAP) observed 24 hours following change. Curvature-directed only occurred delay induction differentiation coincided with spatially restricted YAP localization, indicating initiate curvature–dependent temporally regulate

Language: Английский

Citations

53
Chemical reprogramming for cell fate manipulation: Methods, applications, and perspectives DOI Creative Commons

Jinlin Wang,

Shicheng Sun, Hongkui Deng

et al.

Cell stem cell, Journal Year: 2023, Volume and Issue: 30(9), P. 1130 - 1147

Published: Aug. 24, 2023

Language: Английский

Citations

48
Trends in mechanobiology guided tissue engineering and tools to study cell-substrate interactions: a brief review DOI Creative Commons
Arun Rajendran,

D. Sankar,

Sivashanmugam Amirthalingam

et al.

Biomaterials Research, Journal Year: 2023, Volume and Issue: 27(1)

Published: Feb. 9, 2023

Sensing the mechanical properties of substrates or matrix by cells and tissues, subsequent downstream responses at cellular, nuclear epigenetic levels outcomes are beginning to get unraveled more recently. There have been various instances where researchers established underlying connection between cellular mechanosignalling pathways physiology, differentiation, also tissue pathology. It has now accepted that mechanosignalling, alone in combination with classical pathways, could play a significant role fate determination, development, organization tissues. Furthermore, as mechanobiology is gaining traction, so do techniques ponder gain insights into still pathways. This review would briefly discuss some interesting works wherein it shown specific alteration lead determination stem differentiated such osteoblasts, adipocytes, tenocytes, cardiomyocytes, neurons, how these being utilized for development organoids. cover developed employed explore effects including imaging mechanosensing proteins, atomic force microscopy (AFM), quartz crystal microbalance dissipation measurements (QCMD), traction (TFM), microdevice arrays, Spatio-temporal image analysis, optical tweezer measurements, mechanoscanning ion conductance (mSICM), acoustofluidic interferometric device (AID) forth. provide who work on exploiting control functions engineering regenerative applications, will shed light advancements be unravel unknown field mechanobiology.

Language: Английский

Citations

43
Human organoids with an autologous tissue-resident immune compartment DOI Creative Commons
Timothy Recaldin, Linda Steinacher,

Bruno Gjeta

et al.

Nature, Journal Year: 2024, Volume and Issue: 633(8028), P. 165 - 173

Published: Aug. 14, 2024

The intimate relationship between the epithelium and immune system is crucial for maintaining tissue homeostasis, with perturbations therein linked to autoimmune disease cancer

Language: Английский

Citations

34
Integrating organoids and organ-on-a-chip devices DOI
Yimu Zhao, Shira Landau, Sargol Okhovatian

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(7), P. 588 - 608

Published: July 2, 2024

Language: Английский

Citations

32
Human organoids-on-chips for biomedical research and applications DOI Creative Commons
Hui Wang,

Xiufan Ning,

Feng Zhao

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(2), P. 788 - 818

Published: Jan. 1, 2024

Human organoids-on-chips (OrgOCs) are the synergism of human organoids (HOs) technology and microfluidic organs-on-chips (OOCs).OOCs can mimic extrinsic characteristics organs, such as environmental clues living tissue, while HOs more amenable to biological analysis genetic manipulation.By spatial cooperation, OrgOCs served 3D organotypic models allowing them recapitulate critical tissue-specific properties forecast responses outcomes.It represents a giant leap forward from regular 2D cell monolayers animal in improved ecological niche modeling.In recent years, have offered potential promises for clinical studies advanced preclinical-to-clinical translation medical industrial fields.In this review, we highlight cutting-edge achievements OrgOCs, introduce key features architectures, share revolutionary applications basic biology, disease modeling, preclinical assay precision medicine.Furthermore, discuss how combine wide range disciplines with accelerate translational applications, well challenges opportunities biomedical research applications.

Language: Английский

Citations

28