Biomaterials / bioinks and extrusion bioprinting

Bioactive Materials, Journal Year: 2023, Volume and Issue: 28, P. 511 - 536

Published: June 27, 2023

Bioinks are formulations of biomaterials and living cells, sometimes with growth factors or other biomolecules, while extrusion bioprinting is an emerging technique to apply deposit these bioinks biomaterial solutions create three-dimensional (3D) constructs architectures mechanical/biological properties that mimic those native human tissue organs. Printed have found wide applications in engineering for repairing treating tissue/organ injuries, as well vitro modelling testing validating newly developed therapeutics vaccines prior their use humans. Successful printing subsequent rely on the formulated bioinks, including rheological, mechanical, biological properties, process. This article critically reviews latest developments bioprinting, focusing bioink synthesis characterization, influence Key issues challenges also discussed along recommendations future research.

Language: Английский

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Light-based vat-polymerization bioprinting

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: June 22, 2023

Language: Английский

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The Concept of Scaffold-Guided Bone Regeneration for the Treatment of Long Bone Defects: Current Clinical Application and Future Perspective

Journal of Functional Biomaterials, Journal Year: 2023, Volume and Issue: 14(7), P. 341 - 341

Published: June 27, 2023

The treatment of bone defects remains a challenging clinical problem with high reintervention rates, morbidity, and resulting significant healthcare costs. Surgical techniques are constantly evolving, but outcomes can be influenced by several parameters, including the patient’s age, comorbidities, systemic disorders, anatomical location defect, surgeon’s preference experience. most used therapeutic modalities for regeneration long include distraction osteogenesis (bone transport), free vascularized fibular grafts, Masquelet technique, allograft, (arthroplasty with) mega-prostheses. Over past 25 years, three-dimensional (3D) printing, breakthrough layer-by-layer manufacturing technology that produces final parts directly from 3D model data, has taken off transformed enabling personalized therapies highly porous 3D-printed implants tailored to patient. Therefore, reduce morbidities complications associated current regimens, efforts have been made in translational research toward scaffolds facilitate regeneration. Three-dimensional printed should not only provide osteoconductive surfaces cell attachment subsequent formation also physical support containment graft material during process, enhancing ingrowth, while simultaneously, orthopaedic supply mechanical strength rigid, stable external and/or internal fixation. In this perspective review, we focus on elaborating history defect methods assessing approaches as well recent developments, existing evidence advantages disadvantages Furthermore, it is evident regulatory framework organization financing evidence-based trials very complex, new challenges non-biodegradable biodegradable emerging yet sufficiently addressed, such guideline development specific surgical indications, clinically feasible design concepts needed multicentre international preclinical trials, medico-legal status, reimbursement. These underscore need intensive exchange open honest debate among leaders field. This goal addressed well-planned focused stakeholder workshop topic patient-specific regeneration, proposed review.

Language: Английский

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3D and 4D Bioprinting Technologies: A Game Changer for the Biomedical Sector?

Annals of Biomedical Engineering, Journal Year: 2023, Volume and Issue: 51(8), P. 1683 - 1712

Published: June 1, 2023

Language: Английский

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Volumetric Printing Across Melt Electrowritten Scaffolds Fabricates Multi‐Material Living Constructs with Tunable Architecture and Mechanics

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(32)

Published: April 26, 2023

Major challenges in biofabrication revolve around capturing the complex, hierarchical composition of native tissues. However, individual 3D printing techniques have limited capacity to produce composite biomaterials with multi-scale resolution. Volumetric bioprinting recently emerged as a paradigm-shift biofabrication. This ultrafast, light-based technique sculpts cell-laden hydrogel bioresins into structures layerless fashion, providing enhanced design freedom over conventional bioprinting. it yields prints low mechanical stability, since soft, cell-friendly hydrogels are used. Herein, possibility converge volumetric melt electrowriting, which excels at patterning microfibers, is shown for fabrication tubular hydrogel-based composites behavior. Despite including non-transparent electrowritten scaffolds process, high-resolution bioprinted successfully achieved. Tensile, burst, and bending properties printed tubes tuned altering mesh design, resulting multi-material constructs customizable, anisotropic geometries that better mimic intricate biological structures. As proof-of-concept, engineered obtained by building trilayered vessels, features (valves, branches, fenestrations) can be rapidly using this hybrid approach. multi-technology convergence offers new toolbox manufacturing mechanically tunable living

Language: Английский

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Emerging Bioprinting for Wound Healing

Advanced Materials, Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 11, 2023

Bioprinting has attracted much attention due to its suitability for fabricating biomedical devices. In particular, bioprinting become one of the growing centers in field wound healing, with various types bioprinted devices being developed, including 3D scaffolds, microneedle patches, and flexible electronics. Bioprinted can be designed specific biostructures biofunctions that closely match shape sites accelerate regeneration skin through approaches. Herein, a comprehensive review smart dressings is presented, emphasizing crucial effect determining biofunctions. The begins an overview techniques devices, followed in-depth discussion polymer-based inks, modification strategies, additive ingredients, properties, applications. strategies are divided into seven categories, chemical synthesis novel physical blending, coaxial bioprinting, multimaterial absorption, immobilization, hybridization living cells, examples presented. Thereafter, frontiers 4D artificial intelligence-assisted situ discussed from perspective interdisciplinary sciences. Finally, current challenges future prospects this highlighted.

Language: Английский

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Recent Advances in 3D Printing of Smart Scaffolds for Bone Tissue Engineering and Regeneration

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(34)

Published: June 11, 2024

The repair and functional reconstruction of bone defects resulting from severe trauma, surgical resection, degenerative disease, congenital malformation pose significant clinical challenges. Bone tissue engineering (BTE) holds immense potential in treating these defects, without incurring prevalent complications associated with conventional autologous or allogeneic grafts. 3D printing technology enables control over architectural structures at multiple length scales has been extensively employed to process biomimetic scaffolds for BTE. In contrast inert grafts, next-generation smart possess a remarkable ability mimic the dynamic nature native extracellular matrix (ECM), thereby facilitating regeneration. Additionally, they can generate tailored controllable therapeutic effects, such as antibacterial antitumor properties, response exogenous and/or endogenous stimuli. This review provides comprehensive assessment progress 3D-printed BTE applications. It begins an introduction physiology, followed by overview technologies utilized scaffolds. Notable advances various stimuli-responsive strategies, efficacy, applications are discussed. Finally, highlights existing challenges development implementation scaffolds, well emerging this field.

Language: Английский

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Spatial‐Selective Volumetric 4D Printing and Single‐Photon Grafting of Biomolecules within Centimeter‐Scale Hydrogels via Tomographic Manufacturing

Advanced Materials Technologies, Journal Year: 2023, Volume and Issue: 8(15)

Published: May 23, 2023

Conventional additive manufacturing and biofabrication techniques are unable to edit the chemicophysical properties of printed object postprinting. Herein, a new approach is presented, leveraging light-based volumetric printing as tool spatially pattern any biomolecule interest in custom-designed geometries even across large, centimeter-scale hydrogels. As biomaterial platform, gelatin norbornene resin developed with tunable mechanical suitable for tissue engineering applications. The can be volumetrically within seconds at high resolution (23.68 ± 10.75 μm). Thiol-ene click chemistry allows on-demand photografting thiolated compounds postprinting, from small large (bio)molecules (e.g., fluorescent dyes or growth factors). These molecules covalently attached into structures using light projections, forming 3D spatiotemporal control ≈50 μm resolution. proof concept, vascular endothelial factor locally photografted bioprinted construct demonstrated region-dependent enhanced adhesion network formation cells. This technology paves way toward precise biofunctionalization modification chemical composition (bio)printed constructs better guide cell behavior, build bioactive cue gradients. Moreover, it opens future possibilities 4D mimic dynamic changes morphogen presentation natively experienced biological tissues.

Language: Английский

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3D Bioprinting in Microgravity: Opportunities, Challenges, and Possible Applications in Space

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(23)

Published: June 23, 2023

Abstract 3D bioprinting has developed tremendously in the last couple of years and enables fabrication simple, as well complex, tissue models. The international space agencies have recognized unique opportunities these technologies for manufacturing cell models basic research space, particular investigating effects microgravity cosmic radiation on different types human tissues. In addition, is capable producing clinically applicable grafts, its implementation therefore can support autonomous medical treatment options astronauts future long term far‐distant missions. article discusses but also challenges operating bioprinters under conditions, mainly microgravity. While some process steps, most which involving handling liquids, are challenging microgravity, this environment help overcome problems such sedimentation low viscous bioinks. Hopefully, publication will motivate more researchers to engage topic, with publicly available becoming at International Space Station (ISS) imminent future.

Language: Английский

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Recent progress of 3D printed vascularized tissues and organs

Smart Materials in Medicine, Journal Year: 2024, Volume and Issue: 5(2), P. 183 - 195

Published: Jan. 12, 2024

Since the need for vascular networks to supply oxygen and nutrients while expelling metabolic waste, most cells can only survive within 200 μm of blood vessels; thus, construction well-developed vessel is essential manufacture artificial tissues organs. Three-dimensional (denoted as 3D) printing a scalable, reproducible high-precision manufacturing technology. In past several years, there have been many breakthroughs in building various vascularized tissues, greatly promoting development biological tissue engineering. This paper highlights latest progress 3D printed organs, including heart, liver, lung, kidney, penis. We also discuss application status potential above prospect further requirement technology clinically useable tissues.

Language: Английский

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