Upregulation of LINC00963 facilitates melanoma progression through miR-608/NACC1 pathway and predicts poor prognosis

Biochemical and Biophysical Research Communications, Journal Year: 2018, Volume and Issue: 504(1), P. 34 - 39

Published: Sept. 1, 2018

Language: Английский

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Therapeutic potential of extracellular vesicle‐associated long noncoding RNA

Bioengineering & Translational Medicine, Journal Year: 2020, Volume and Issue: 5(3)

Published: July 10, 2020

Abstract Both extracellular vesicles (EVs) and long noncoding RNAs (lncRNAs) have been increasingly investigated as biomarkers, pathophysiological mediators, potential therapeutics. While these two entities often studied separately, there are increasing reports of EV‐associated lncRNA activity in processes such oncogenesis well tissue repair regeneration. Given the powerful nature emerging translational impact other microRNA (miRNA) small interfering RNA, therapeutics may represent a new frontier. EVs natural vehicles that transport protect lncRNAs physiologically, they can also be engineered for enhanced cargo loading therapeutic properties. In this review, we will summarize relevant to both cancer treatment discuss role enabling

Language: Английский

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Accuracy and safety of robot-assisted cortical bone trajectory screw placement: a comparison of robot-assisted technique with fluoroscopy-assisted approach

BMC Musculoskeletal Disorders, Journal Year: 2022, Volume and Issue: 23(1)

Published: April 6, 2022

Abstract Objective To compare the safety and accuracy of cortical bone trajectory screw placement between robot-assisted fluoroscopy-assisted approaches. Methods This retrospective study was conducted November 2018 June 2020, including 81 patients who underwent (CBT) surgery for degenerative lumbar spine disease. CBT performed by same team experienced surgeons. The were randomly divided into two groups—the group (FA, 44 patients) (RA, 37 patients). Robots orthopedic used in , whereas conventional fluoroscopy-guided group. rate superior facet joint violation assessed using postoperative computed tomography (CT). time single placement, intraoperative blood loss, radiation exposure to surgical also recorded. χ 2 test Student’s t-test analyze significance variables ( P < 0.05). Results A total 376 screws inserted patients, 172 204 pedicle Screw higher RA (160, 93%) than FA (169, 83%) = 0.003). had a lower number reaching grade 0 (58, 78%) more that (56, 64%) 0.041). longer (7.25 ± 0.84 min) (5.58 1.22 min, 0.001). bleeding (273.41 118.20 ml) (248.65 97.53 ml, 0.313). (0.43 0.07 (0.37 0.10 Furthermore, overall learning curve tended decrease. Conclusions Robot-assisted improves accuracy, shortens time, effectively efficiency, reduces team. In addition, is smooth easy operate.

Language: Английский

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Long Non-Coding RNA MEG3 in Metal Carcinogenesis

Toxics, Journal Year: 2023, Volume and Issue: 11(2), P. 157 - 157

Published: Feb. 7, 2023

Most transcripts from human genomes are non-coding RNAs (ncRNAs) that not translated into proteins. ncRNAs divided long (lncRNAs) and small (sncRNAs). LncRNAs regulate their target genes both transcriptionally post-transcriptionally through interactions with proteins, RNAs, DNAs. Maternally expressed gene 3 (MEG3), a lncRNA, functions as tumor suppressor. MEG3 regulates cell proliferation, cycle, apoptosis, hypoxia, autophagy, many other processes involved in development. is downregulated various cancer lines primary cancers. Heavy metals, such hexavalent chromium (Cr(VI)), arsenic, nickel, cadmium, confirmed carcinogens. The exposure of cells to these metals causes variety Among them, lung the one can be induced by all metals. In vitro studies have demonstrated chronic normal bronchial epithelial (BEAS-2B) cause malignant transformation. Metal-transformed capability an increase resistance elevated migration invasion, properties stem-like cells. Studies revealed MEG Cr(VI)-transformed cells, nickel-transformed cadmium (Cd)-transformed forced expression reduces invasion downregulation neuronal precursor developmentally protein 9 (NEDD9). suppresses transformation overexpression decreases Bcl-xL, causing reduced apoptosis Cd-transformed This paper reviews current knowledge lncRNA metal carcinogenesis.

Language: Английский

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Propranolol inhibits EMT and metastasis in breast cancer through miR-499-5p-mediated Sox6

Journal of Cancer Research and Clinical Oncology, Journal Year: 2024, Volume and Issue: 150(2)

Published: Jan. 31, 2024

Abstract Purpose This study will focus on 4T1 cells, a murine mammary adenocarcinoma cell line, as the primary research subject. We aim to investigate inhibitory effects and mechanisms of propranolol epithelial–mesenchymal transition (EMT) in breast cancer aiming elucidate this phenomenon at miRNA level. Methods In study, EMT effect was observed through vitro animal experiments. For screening potential target miRNAs downstream genes, second-generation sequencing (SGS) bioinformatics analysis were conducted. Following process, identified their respective genes confirmed using various experimental methods. To confirm Western Blot (WB), reverse transcription polymerase chain reaction (RT-PCR), immunofluorescence experiments performed. Results we found that significantly reduced lung metastasis cells ( p < 0.05). vivo demonstrated inhibited evidenced by Through next-generation (SGS), subsequent analysis, PCR validation, marked downregulation miR-499-5p 0.05), suggesting its involvement mediating suppressive EMT. Overexpression promoted EMT, migration, invasion these not reversed or attenuated (Validated via Blot, wound healing assay, transwell assays, Sox6 functional miR-499-5p, with correlating changes Silencing overexpressing expression, further promoting processes invasion, migration cells. Notably, alleviated (validated The direct interaction between mRNA dual-luciferase reporter gene assay. Conclusion These results suggest may have therapeutic agent for treatment targeting regulatory mechanisms.

Language: Английский

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Inhibition of breast cancer stem-like cells by a triterpenoid, ursolic acid, via activation of Wnt antagonist, sFRP4 and suppression of miRNA-499a-5p

Life Sciences, Journal Year: 2020, Volume and Issue: 265, P. 118854 - 118854

Published: Dec. 2, 2020

Language: Английский

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Long non‑coding RNA MEG3 inhibits cell migration and invasion of non‑small cell lung cancer cells by regulating the miR‑21‑5p/PTEN axis

Molecular Medicine Reports, Journal Year: 2021, Volume and Issue: 23(3)

Published: Jan. 6, 2021

Long non‑coding RNAs (lncRNAs) are involved in the occurrence and progression of numerous types cancer. The aim present study was to evaluate effect lncRNA maternally expressed gene 3 (MEG3) on migration invasion non‑small cell lung cancer (NSCLC) H1299 PC9 cells. Reverse transcription‑quantitative (RT‑q)PCR analysis showed that MEG3 downregulated NSCLC Additionally, bioinformatics indicated sponges microRNA (miR)‑21‑5p; miR‑21‑5p predicted target phosphatase tensin homolog (PTEN) 3'‑untranslated region sequence. overexpression led suppression PTEN upregulation cells, as detected by RT‑qPCR. Subsequently, western blot confirmed enhanced expression levels inhibited PI3K/AKT signaling pathway These effects were attenuated miR‑21‑5p. Dual luciferase assay supported sponging validated direct interaction between PTEN. Furthermore, Transwell demonstrated had an inhibitory invasion. also mediated epithelial‑to‑mesenchymal transition significantly enhancing E‑cadherin decreasing N‑cadherin, Vimentin matrix metalloprotein 9 analysis. changes partially reversed mimic. results acted a tumor suppressor via miR‑21‑5p, which, turn, PTEN, part pathway. have suggested potential novel therapeutic for treatment.

Language: Английский

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CircMRP4 orchestrates podocytes injury via the miR-499-5p/RRAGB/mTORC1 axis in diabetic kidney disease

Cellular Signalling, Journal Year: 2025, Volume and Issue: 127, P. 111611 - 111611

Published: Jan. 20, 2025

Language: Английский

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The critical roles of lncRNAs in the pathogenesis of melanoma

Experimental and Molecular Pathology, Journal Year: 2020, Volume and Issue: 117, P. 104558 - 104558

Published: Oct. 21, 2020

Language: Английский

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Cytoglobin inhibits non-thermal plasma-induced apoptosis in melanoma cells through regulation of the NRF2-mediated antioxidant response

Redox Biology, Journal Year: 2022, Volume and Issue: 55, P. 102399 - 102399

Published: July 14, 2022

Melanoma arises from pigment-producing cells called melanocytes located in the basal layers of epidermis skin. Cytoglobin (CYGB) is a ubiquitously expressed hexacoordinated globin that highly enriched and frequently downregulated during melanomagenesis. Previously, we showed non-thermal plasma (NTP)-produced reactive oxygen nitrogen species (RONS) lead to formation an intramolecular disulfide bridge would allow CYGB function as redox-sensitive protein. Here, investigate cytotoxic effect indirect NTP treatment two melanoma cell lines with divergent endogenous expression levels, explore role determining outcome. Our findings are consistent previous studies supporting cytotoxicity mediated through production RONS leads apoptotic death cells. Furthermore, show NTP-treated solutions elicit antioxidant response activation nuclear factor erythroid 2-related 2 (NRF2). The knockdown overexpression respectively sensitizes protects RONS-induced death. presence enhances heme-oxygenase 1 (HO-1) NRF2 protein whereas absence impairs their expression. Moreover, analysis CYGB-dependent transcriptome demonstrates tumor suppressor long non-coding RNA maternally 3 (MEG3) hitherto undescribed link between NRF2. Thus, CYGB, at least cells, seems play central therapeutic outcome RONS-inducing anticancer therapies, like solutions, possessing both tumor-suppressive oncogenic features. Hence, could be interest either biomarker or candidate for future targeted therapies melanoma.

Language: Английский

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