Role of miRNA and lncRNAs in organ fibrosis and aging

Biomedicine & Pharmacotherapy, Journal Year: 2021, Volume and Issue: 143, P. 112132 - 112132

Published: Sept. 1, 2021

Fibrosis is the endpoint of pathological remodeling. This process contributes to pathogenesis several chronic disorders and aging-associated organ damage. Different molecular cascades contribute this process. TGF-β, WNT, YAP/TAZ signaling pathways have prominent roles in A number long non-coding RNAs microRNAs been found regulate fibrosis through modulation activity related pathways. miR-144-3p, miR-451, miR-200b, miR-328 are among that participate pathology cardiac fibrosis. Meanwhile, miR-34a, miR-17-5p, miR-122, miR-146a, miR-350 liver different situations. PVT1, MALAT1, GAS5, NRON, PFL, MIAT, HULC, ANRIL, H19 We review impact aging-related pathologies.

Language: Английский

---

Histone Modifications and Non-Coding RNAs: Mutual Epigenetic Regulation and Role in Pathogenesis

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(10), P. 5801 - 5801

Published: May 22, 2022

In the last few years, more and scientists have suggested confirmed that epigenetic regulators are tightly connected form a comprehensive network of regulatory pathways feedback loops. This is particularly interesting for better understanding processes occur in development progression various diseases. Appearing on preclinical stages diseases, aberrations may be prominent biomarkers. Being dynamic reversible, modifications could become targets novel option therapy. Therefore, this review, we focusing histone ncRNAs, their mutual regulation, role cellular potential clinical application.

Language: Английский

---

A systematic review of epigenetic interplay in kidney diseases: Crosstalk between long noncoding RNAs and methylation, acetylation of chromatin and histone

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 176, P. 116922 - 116922

Published: June 13, 2024

The intricate crosstalk between long noncoding RNAs (lncRNAs) and epigenetic modifications such as chromatin/histone methylation acetylation offer new perspectives on the pathogenesis treatment of kidney diseases. lncRNAs, a class transcripts longer than 200 nucleotides with no protein-coding potential, are now recognized key regulatory molecules influencing gene expression through diverse mechanisms. They modulate by recruiting or blocking enzymes responsible for adding removing methyl acetyl groups, DNA, N6-methyladenosine (m6A) histone acetylation, subsequently altering chromatin structure accessibility. In diseases acute injury (AKI), chronic disease (CKD), diabetic nephropathy (DN), glomerulonephritis (GN), renal cell carcinoma (RCC), aberrant patterns DNA/RNA/histone have been associated onset progression, revealing complex interplay lncRNA dynamics. Recent studies highlighted how lncRNAs can impact pathology affecting function genes involved in cycle control, fibrosis, inflammatory responses. This review will separately address roles diseases, particular emphasis elucidating bidirectional effects underlying mechanisms conjunction addition to potential exacerbating renoprotective pathologies. Understanding reciprocal relationships not only shed light molecular underpinnings pathologies but also present avenues therapeutic interventions biomarker development, advancing precision medicine nephrology.

Language: Английский

---

The Role of microRNAs in Metabolic Syndrome-Related Oxidative Stress

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(18), P. 6902 - 6902

Published: Sept. 20, 2020

Oxidative stress (OxS) is the cause and consequence of metabolic syndrome (MetS), incidence economic burden which increasing each year. OxS triggers dysregulation signaling pathways associated with metabolism epigenetics, including microRNAs, are biomarkers disorders. In this review, we aimed to summarize current knowledge regarding interplay between microRNAs in MetS its components. We searched PubMed Google Scholar most relevant studies. Collected data suggested that different sources (e.g., hyperglycemia, insulin resistance (IR), hyperlipidemia, obesity, proinflammatory cytokines) change expression numerous organs involved regulation glucose lipid endothelium. Dysregulated either directly or indirectly affect and/or activity molecules antioxidative (SIRT1, FOXOs, Keap1/Nrf2) along effector enzymes GPx-1, SOD1/2, HO-1), ROS producers NOX4/5), as well genes connected inflammation, sensitivity, metabolism, thus promoting progression imbalance. MicroRNAs appear be important epigenetic modifiers managing delicate redox balance, mediating pro- antioxidant biological impacts. Summarizing, may promising therapeutic targets ameliorating repercussions MetS.

Language: Английский

---

LncRNA SOX2OT alleviates mesangial cell proliferation and fibrosis in diabetic nephropathy via Akt/mTOR-mediated autophagy

Molecular Medicine, Journal Year: 2021, Volume and Issue: 27(1)

Published: July 8, 2021

Accumulating evidences have demonstrated that long non-coding RNAs (lncRNAs) are involved in the pathophysiology of diabetic nephropathy (DN). lncRNA SOX2OT plays an essential role many diseases, including diabetes. Herein, we aim to investigate underlying mechanism DN pathogenesis. Streptozotocin-induced mouse models and high glucose-induced mesangial cells were constructed examine expression pattern SOX2OT. The activation autophagy was evaluated using immunohistochemistry, immunofluorescence western blot analysis, respectively. overexpressing plasmid applied further verify functional CCK-8 EDU assays performed proliferation cells. Additionally, rapamycin, inhibitor mTOR signaling, used clarify whether controls development through Akt/mTOR pathway. markedly down-regulated both streptozotocin-induced mice Moreover, overexpression able diminish suppression alleviate DN-induced renal injury. Functionally, indicated significantly suppressed fibrosis obvious inhibition also observed with overexpression, which then verified vivo. In summary, alleviates pathogenesis via regulating Akt/mTOR-mediated autophagy, may provide a novel target for therapy.

Language: Английский

---

Noncoding RNAs involved in DNA methylation and histone methylation, and acetylation in diabetic vascular complications

Pharmacological Research, Journal Year: 2021, Volume and Issue: 170, P. 105520 - 105520

Published: Feb. 25, 2021

Language: Английский

---

Jiedu Tongluo Baoshen formula enhances renal tubular epithelial cell autophagy to prevent renal fibrosis by activating SIRT1/LKB1/AMPK pathway

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 160, P. 114340 - 114340

Published: Feb. 3, 2023

Renal fibrosis, an important pathological change in the development of diabetic kidney disease (DKD), urgently needs new treatment methods clinically. The Jiedu Tongluo Baoshen (JTBF) formula was created based on theory toxic damage to collaterals, and a variety active ingredients JTBF have inhibitory effects epithelial-mesenchymal transition (EMT) extracellular matrix (ECM). In this study, Ultra Performance Liquid Chromatography (UPLC) employed analyze effective formula. After screening PubChem database, we identified 94 compounds predicted SIRT1 pathway as potential targets through network pharmacology. addition, high fat diet (HFD)+Streptozocin (STZ)-induced DKD rat model glucose (HG)-induced NRK-52E cell model, activates phosphorylation LKB1 AMPK enhances autophagy activity cells, thereby reducing accumulation EMT ECM. These results been confirmed vivo vitro experiments. renal tubular epithelial cells inhibits progression fibrosis by activating SIRT1/LKB1/AMPK signal pathway. This study provides insights into molecular mechanism prevent treat fibrosis.

Language: Английский

---

LncRNA SNHG1 knockdown inhibits hyperglycemia induced ferroptosis viamiR‐16‐5p/ACSL4 axis to alleviate diabetic nephropathy

Journal of Diabetes Investigation, Journal Year: 2023, Volume and Issue: 14(9), P. 1056 - 1069

Published: June 14, 2023

Hyperglycemia accelerates the development of diabetic nephropathy (DN) by inducing renal tubular injury. Nevertheless, mechanism has not been elaborated fully. Here, pathogenesis DN was investigated to seek novel treatment strategies.A model established in vivo, levels blood glucose, urine albumin creatinine ratio (ACR), creatinine, urea nitrogen (BUN), malondialdehyde (MDA), glutathione (GSH), and iron were measured. The expression detected qRT-PCR Western blotting. H&E, Masson, PAS staining used assess kidney tissue mitochondria morphology observed transmission electron microscopy (TEM). molecular interaction analyzed using a dual luciferase reporter assay.SNHG1 ACSL4 increased tissues mice, but miR-16-5p decreased. Ferrostatin-1 or SNHG1 knockdown inhibited ferroptosis high glucose (HG)-treated HK-2 cells db/db mice. Subsequently, confirmed be target for SNHG1, directly targeted ACSL4. Overexpression greatly reversed protective roles HG-induced cells.SNHG1 via miR-16-5p/ACSL4 axis alleviate nephropathy, which provided some new insights nephropathy.

Language: Английский

---

Regulation of NcRNA-protein binding in diabetic foot

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 160, P. 114361 - 114361

Published: Feb. 6, 2023

Non-coding RNA (ncRNA) is a special type of transcript that makes up more than 90 % the human genome. Although ncRNA typically does not encode proteins, it indirectly controls wide range biological processes, including cellular metabolism, development, proliferation, transcription, and post-transcriptional modification. NcRNAs include small interfering (siRNA), PIWI-interacting (piRNA), tRNA-derived (tsRNA), etc. The most researched these are miRNA, lncRNA, circRNA, which crucial regulators in onset diabetes development associated consequences. ncRNAs indicated above linked to numerous problems by binding diabetic foot (DF), nephropathy, cardiomyopathy, peripheral neuropathy. According recent studies, Mir-146a can control AKAP12 axis promote proliferation migration ulcer (DFU) cells, while lncRNA GAS5 activate HIF1A/VEGF pathway TAF15 DFU wound healing. However, there still many unanswered questions about mechanism action ncRNAs. In this study, we explored new progress ncRNA-protein DF, provide help guidance for application early diagnosis potential targeted intervention DFU.

Language: Английский

---

Podocyte-derived extracellular vesicles mediate renal proximal tubule cells dedifferentiation via microRNA-221 in diabetic nephropathy

Molecular and Cellular Endocrinology, Journal Year: 2020, Volume and Issue: 518, P. 111034 - 111034

Published: Sept. 12, 2020

Language: Английский

---