Food Science of Animal Products, Journal Year: 2024, Volume and Issue: 2(3), P. 9240078 - 9240078
Published: Sept. 1, 2024
Language: Английский
Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)
Published: June 14, 2024
Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.
Language: Английский
Citations
39
Toxins, Journal Year: 2025, Volume and Issue: 17(1), P. 36 - 36
Published: Jan. 14, 2025
Extracellular vesicles (EVs) are nanoparticle-sized secreted by nearly all cell types under normal physiological conditions. In toxicological research, EVs have emerged as a crucial link between public health and multi-omics approaches, offering insights into cellular responses to disease-causing injury agents such environmental biological toxins, contaminants, drugs. Notably, present unique opportunity deepen our understanding of the pathophysiology envenomation natural toxins. Recent advancements in isolating purifying EV cargo, mass spectrometry techniques, bioinformatics positioned potential biomarkers that could elucidate signaling pathways provide valuable information on relationship venomous their mechanisms action, effectiveness antivenoms. Additionally, hold promise proxies for various aspects envenomation, including toxin dosage, characterization, progression, prognosis during therapeutic interventions. These can be explored through technology applied contents from plasma, saliva, or urine samples envenomated individuals, comprehensive integrative approach managing cases.
Language: Английский
Citations
2
Acta Pharmaceutica Sinica B, Journal Year: 2023, Volume and Issue: 13(8), P. 3238 - 3251
Published: May 23, 2023
Emerging evidence has demonstrated the vital role of metabolism in various diseases or disorders. Metabolomics provides a comprehensive understanding biological systems. With advanced analytical techniques, metabolomics exhibits unprecedented significant value basic drug research, including disease mechanisms, identifying targets, and elucidating mode action drugs. More importantly, greatly accelerates development process by predicting pharmacokinetics, pharmacodynamics, response. In addition, facilitates exploration repurposing drug-drug interactions, as well personalized treatment strategies. Here, we briefly review recent advances technologies update our knowledge applications research development.
Language: Английский
Citations
36
Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(6), P. 1639 - 1639
Published: June 1, 2023
Extracellular vesicles (EVs) are a heterogeneous class of cell-derived membrane released by various cell types that serve as mediators intercellular signaling. When into circulation, EVs may convey their cargo and intermediaries for intracellular communication, reaching nearby cells possibly also distant organs. In cardiovascular biology, activated or apoptotic endothelial (EC-EVs) disseminate biological information at short long distances, contributing to the development progression disease related disorders. The significance EC-EVs cell-cell communication has advanced, but thorough knowledge role intercommunication plays in healthy vascular is still lacking. Most data on derive from vitro studies, there little reliable available biodistribution specific homing vivo tissues. Molecular imaging techniques crucial monitoring networks both basal pathological circumstances. This narrative review provides an overview EC-EVs, trying highlight messengers interaction homeostasis disease, describes emerging applications modalities visualization vivo.
Language: Английский
Citations
23
Extracellular Vesicles and Circulating Nucleic Acids, Journal Year: 2024, Volume and Issue: 5(2), P. 165 - 79
Published: April 28, 2024
This review article presents a detailed examination of the integral role that electrochemical detection extracellular vesicles (EVs) plays, particularly focusing on potential application for early disease diagnostics through EVs biomarker analysis. Through an exploration benefits and challenges presented by vetted protein, lipid, nucleic acid analysis, we underscore significance these techniques. Evidence from recent studies renders this modality imperative in identifying diverse biomarkers EVs, leading to diagnosis diseases such as cancer neurodegenerative disorders. Recent advancements have led enhanced sensitivity, specificity point-of-care testing (POCT) are elucidated, along with equipment deployed detection. The concludes contemplation future perspectives, recognizing shifts prognosis, necessary advances broad adoption, areas ongoing research. objective is propel further investigation into rapidly burgeoning field, thereby facilitating paradigm shift detection, monitoring, treatment toward human health management.
Language: Английский
Citations
5
Biomedicines, Journal Year: 2024, Volume and Issue: 12(5), P. 992 - 992
Published: April 30, 2024
Extracellular vesicles represent a group of structures with the capacity to communicate different cells and organs. This complex network interactions can regulate multiple physiological processes in organism. Very importantly, these be altered during appearance diseases including cancer, metabolic diseases, etc. In addition, extracellular transport cargoes, altering initiation disease, driving progression, or even accelerating pathogenesis. Then, we have explored implication alterations such as pancreatic diabetes its complications non-alcoholic fatty liver disease. Finally, more detail communication between pancreas. summary, very efficient system for among tissues permit an biomarkers well being involved extracellular-vesicle-mediated molecules, serving potential therapy diseases.
Language: Английский
Citations
5
Journal of the Endocrine Society, Journal Year: 2024, Volume and Issue: 8(8)
Published: June 25, 2024
Abstract Obesity is a major public health issue that associated with metabolic diseases including diabetes mellitus type 2 and syndrome. This pathology leads to detrimental cardiovascular secondary effects, such as lipotoxicity, inflammation, oxidative stress. Recently, extracellular vesicles (EVs) have been highlighted novel players participating in human physiology pathophysiology. In obesity, adipose tissue related the active shedding of adipocyte-derived (AdEVs). The current review explores highlights role AdEVs their cargo obesity are proposed play an important its comorbidities. biological nanoparticles mainly shed by visceral subcutaneous tissue, acting physiological pathophysiological conditions, also carrying different biomolecules, RNA, microRNA (miRNA), proteins, lipids, among others. RNA miRNA local systemic effects affecting gene expression target cell types via paracrine endocrine actions. State art analyses identified some miRNAs, miR-222, miR-23b, miR-4429, miR-148b, miR-4269, could potentially affect pathways involved obesity-related comorbidities, chronic inflammation fibrosis. Similarly, AdEVs-proteins (RBP4, perilipin-A, FABP, mimecan, TGFBI) AdEVs-lipids (sphingolipids) linked knowledge about along further research would support reveal pathways, potential biomarkers, therapeutic options obesity.
Language: Английский
Citations
4
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7237 - 7237
Published: April 14, 2023
Among the extracellular vesicles, apoptotic bodies (ABs) are only formed during apoptosis and perform a relevant role in pathogenesis of different diseases. Recently, it has been demonstrated that ABs from human renal proximal tubular HK-2 cells, either induced by cisplatin or UV light, can lead to further death naïve cells. Thus, aim this work was carry out non-targeted metabolomic approach study if stimulus (cisplatin light) affects way metabolites involved propagation apoptosis. Both their fluid were analyzed using reverse-phase liquid chromatography-mass spectrometry setup. Principal components analysis showed tight clustering each experimental group partial least square discriminant used assess metabolic differences existing between these groups. Considering variable importance projection values, molecular features selected some them could be identified unequivocally tentatively. The resulting pathways indicated there significant, stimulus-specific abundancies may propagate healthy cells; thus, we hypothesize share might vary depending on stimulus.
Language: Английский
Citations
10
Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 116 - 116
Published: Jan. 8, 2025
Pseudomonas aeruginosa and Mycobacterium abscessus are opportunistic pathogens that cause severe infections in hospitals, their co-infections increasingly reported. The interspecies interactions between these two bacterial species potential impacts on largely unexplored. In this study, we first demonstrated P. inhibits the growth of M. by iron chelating via pqs quorum sensing. Next, through proteomic analysis, discovered PQS molecule significantly changed a large amount protein expression abscessus, including proteins involved type VII secretion system homeostasis. Furthermore, revealed enhanced production membrane vesicles (MVs) abscessus. Our study suggests can serve as an signaling to communicate with affect physiology virulence.
Language: Английский
Citations
0
Cancer Letters, Journal Year: 2025, Volume and Issue: unknown, P. 217531 - 217531
Published: Feb. 1, 2025
Language: Английский
Citations
0