Role of oxidative stress in male infertility

Reproduction and Fertility, Journal Year: 2023, Volume and Issue: 4(3)

Published: June 5, 2023

Graphical abstract Abstract Infertility affects millions of couples worldwide. Oxidative stress (OS) causes peroxidation lipids and damage to spermatozoa, thus, reducing the quality seminal parameters. In addition, differences in levels antioxidants reactive oxygen species (ROS) caused by intrinsic extrinsic variables linked lifestyle, diet, genetics, OS also contribute male infertility. High ROS result sperm parameters due lipid oxidation proteins. Other significant include changes sex hormone levels, DNA damage, including mutations, immature spermatozoa. Treating root OS, changing one’s as well antioxidant therapy, may be helpful strategies fight OS-related However, determination infertility induced is currently a challenge field reproductive health research. This review intends describe role oxidative on current understanding its management. Lay summary The inability conceive many globally. refers imbalances between different which can lead fertility problems damaging semen. several factors, diets high fats, sugars processed foods, lifestyle (including smoking, alcohol consumption having sedentary lifestyle), genetics. Treatment that focuses cause help combat there no consensus best way treat problems, particularly those associated with stress. paper describes discusses techniques employed treating issues.

Language: Английский

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An Overview of Reactive Oxygen Species Damage Occurring during In Vitro Bovine Oocyte and Embryo Development and the Efficacy of Antioxidant Use to Limit These Adverse Effects

Animals, Journal Year: 2024, Volume and Issue: 14(2), P. 330 - 330

Published: Jan. 21, 2024

The in vitro production (IVP) of bovine embryos has gained popularity worldwide and recent years its use for producing from genetically elite heifers cows surpassed the conventional superovulation-based embryo schemes. There are, however, several issues with IVP that remain unresolved. One limitation special concern is low efficiency embryos. Exposure to reactive oxygen species (ROS) one reason why diminished. These highly molecules are generated small amounts through normal cellular metabolism, but their abundances increase culture because oocyte exposure temperature fluctuations, light exposure, pH changes, atmospheric tension, suboptimal media formulations, cryopreservation. When uncontrolled, ROS produce detrimental effects on structure function genomic mitochondrial DNA, alter DNA methylation, lipid membrane damage, modify protein activity. Several intrinsic enzymatic pathways control abundance antioxidants react reduce potential ROS. This review will focus exploring supplementing these antioxidant maturation, sperm viability, fertilization, culture.

Language: Английский

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Antioxidant therapy for infertile couples: a comprehensive review of the current status and consideration of future prospects

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 9, 2025

Oxidative stress (OS) is established as a key factor in the etiology of both male and female infertility, arising from an imbalance between reactive oxygen species (ROS) production endogenous antioxidant (AOX) defenses. In men, OS adversely affects sperm function by inducing DNA damage, reducing motility, significantly impairing vitality through plasma membrane peroxidation loss integrity, ultimately compromising overall quality. women, implicated various reproductive disorders, including polycystic ovary syndrome, endometriosis, premature ovarian failure, leading to diminished oocyte quality, disrupted folliculogenesis, poorer outcomes. Antioxidant therapy represents promising intervention mitigate harmful effects ROS on health additions its easy accessibility, safety, low cost. Despite several findings suggesting improvements fertility potential with AOX therapy, data remains inconclusive regarding optimal dosage combination, duration treatment, specific patient populations most likely benefit. this review, we discuss role AOXs management infertile couples, focusing their biological mechanisms, adverse effects, therapeutic efficacy, clinical applications improving outcomes natural conception medically assisted reproduction. Additionally, highlight current practice patterns recommendations for supplementation during course infertility treatment. Further, provide overview limitations research topic insights future studies establish standardized regimens assess long-term impact such live birth rates miscarriage rates.

Language: Английский

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Oxidative Stress in Assisted Reproductive Techniques, with a Focus on an Underestimated Risk Factor

Current Issues in Molecular Biology, Journal Year: 2023, Volume and Issue: 45(2), P. 1272 - 1286

Published: Feb. 3, 2023

Based on current findings, the presence of oxidative stress has a significant impact quality gametes and embryos when performing assisted reproductive techniques (ART). Unfortunately, in vitro manipulation these cells exposes them to higher level reactive oxygen species (ROS). The primary goal this review is provide comprehensive overview development female male systems, as well case pre-implantation embryo its environment. This also focuses origins ROS mechanisms stress-induced damage during ART procedures. A well-known but underestimated hazard, light exposure-related photo-oxidation, particularly concerning. effect outcomes, various strategies for preventing it, are discussed. We emphasize role significance antioxidants protection including forms, functions, vivo vitro.

Language: Английский

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Redox Homeostasis and Nrf2-Regulated Mechanisms Are Relevant to Male Infertility

Antioxidants, Journal Year: 2024, Volume and Issue: 13(2), P. 193 - 193

Published: Feb. 3, 2024

Infertility represents a significant global health challenge, affecting more than 12% of couples worldwide, and most cases infertility are caused by male factors. Several pathological pathways implicated in infertility. The main mechanisms involved driven the loss reduction–oxidation (redox) homeostasis resulting oxidative damage as well chronic inflammatory process. Increased or severe stress leads to sperm plasma membrane DNA damage, dysregulated RNA processing, telomere destruction. signaling these molecular events also regulated Nuclear factor-E2-related factor 2 (Nrf2). causes infertility, role Keap1-Nrf2 antioxidant pathway reviewed. This review highlights regulatory Nrf2 balance between oxidants antioxidants relevant fertility. is regulation spermatogenesis quality. Establishing link fertility provides basis for modulation processes, reactive oxygen species generation, network, including Nrf2-regulated response, improve reproductive outcomes.

Language: Английский

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The impact of mitochondrial impairments on sperm function and male fertility: a systematic review

Reproductive Biology and Endocrinology, Journal Year: 2024, Volume and Issue: 22(1)

Published: July 17, 2024

Abstract Background Besides adenine triphosphate (ATP) production for sustaining motility, the mitochondria of sperm also host other critical cellular functions during germ cell development and fertilization including calcium homeostasis, generation reactive oxygen species (ROS), apoptosis, in some cases steroid hormone biosynthesis. Normal mitochondrial membrane potential with optimal performance is essential capacitation, acrosome reaction, DNA integrity. Results Defects function can severely harm fertility males. The role its final fate after still controversial. Here, we review current knowledge on human characteristics their physiological pathological conditions, paying special attention to improvements assistant reproductive technology available treatments ameliorate male infertility. Conclusion Although variants associated infertility have clinical use, research limited. Further understanding needed determine how these lead adverse pregnancy outcomes affect potential.

Language: Английский

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Vitrification affects the post-implantation development of mouse embryos by inducing DNA damage and epigenetic modifications

Clinical Epigenetics, Journal Year: 2025, Volume and Issue: 17(1)

Published: Feb. 7, 2025

Vitrification is widely used in assisted reproductive technology (ART) for female infertility, but the long-term effect on embryo of vitrification has not been comprehensively studied. The study aimed to investigate development mouse embryos. warmed embryos which were frozen at 8-cell stage cultured vitro until blastocyst and transferred into recipients. Immunofluorescence staining was performed evaluate reactive oxygen species (ROS) level, mitochondrial function, cell apoptosis, DNA damage histone epigenetic modification blastocysts. Transmission electron microscopy (TEM) analysis exam ultrastructure related gene expression transcriptome profiles investigated by RT-qPCR RNA-seq, respectively. Blastocyst implantation frequencies significantly affected vitrification. However, reduced number live pup frequency. induced ROS accumulation, damage, apoptosis homologous recombination (NHEJ) major repair pathway vitrified elevated H3K4me2/3, H4K12ac, H4K16ac levels m6A Moreover, altered mice placentas brains embryonic day 18.5 (E18.5). Thus, exhibited a viability increasing levels, altering modifications profiles.

Language: Английский

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Mechanisms of action of non-enzymatic antioxidants to control oxidative stress during in vitro follicle growth, oocyte maturation, and embryo development

Animal Reproduction Science, Journal Year: 2022, Volume and Issue: 249, P. 107186 - 107186

Published: Dec. 30, 2022

Language: Английский

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A review of the use of antioxidants in bovine sperm preparation protocols

Animal Reproduction Science, Journal Year: 2023, Volume and Issue: 251, P. 107215 - 107215

Published: March 15, 2023

Language: Английский

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Biosafe cerium oxide nanozymes protect human pluripotent stem cells and cardiomyocytes from oxidative stress

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: March 26, 2024

Abstract Background Cardiovascular diseases (CVDs) have the highest mortality worldwide. Human pluripotent stem cells (hPSCs) and their cardiomyocyte derivatives (hPSC-CMs) offer a valuable resource for disease modeling, pharmacological screening, regenerative therapy. While most CVDs are linked to significant over-production of reactive oxygen species (ROS), effects current antioxidants targeting excessive ROS limited. Nanotechnology is powerful tool develop with improved selectivity, solubility, bioavailability prevent or treat various related oxidative stress. Cerium oxide nanozymes (CeONZs) can effectively scavenge by mimicking activity endogenous antioxidant enzymes. This study aimed assess nanotoxicity CeONZs potential benefits in stressed human embryonic (hESCs) derived cardiomyocytes (hESC-CMs). Results demonstrated reliable nanosafety biocompatibility hESCs hESC-CMs within broad range concentrations. exhibited protective on cell viability alleviating ROS-induced Moreover, protected from doxorubicin (DOX)-induced cardiotoxicity partially ameliorated insults DOX neonatal rat (NRCMs). Furthermore, during culture, were found reduce ROS, decrease apoptosis, enhance survival without affecting self-renewal differentiation potential. Conclusions displayed good safety biocompatibility, as well enhanced shielding them damage. These promising results suggest that may be crucial, safe nanoantioxidant, potentially improve therapeutic efficacy incorporated into medicine. Graphical

Language: Английский

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