COMPARISON OF THE EFFECTS OF DOCETAXEL and AMYGDALIN TREATMENT ON CELL DEATH, INTEGRIN-α and INTEGRIN-β EXPRESSIONS IN DU145 PROSTATE CANCER CELL LINE DOI Open Access
Çiğdem Karaca, Evrim Suna Arıkan Söylemez, Esra Aslan

et al.

Kocatepe tıp dergisi., Journal Year: 2024, Volume and Issue: 25(4), P. 420 - 428

Published: Oct. 21, 2024

OBJECTIVE: Prostate cancer (PC) ranks second among cancer-related deaths in men, and most are caused by metastasis. Integrins, which cell surface receptors, play an important role It has been shown that integrin alpha2beta1 expression is effective adhesion, migration, invasion increasing binding to collagen I metastatic PCs. Docetaxel chemotherapy used PC, but it ineffective advanced stages. Amygdalin a cyanogenic glycoside commonly found fruit seeds, there conflict the literature regarding its effectiveness treatment. We aimed compare effects of treatments on DU145 prostate line integrinalfa2 (ITGA2) integrinbeta1 (ITGB1) expressions, as well their death, Caspase-3, Beclin-1. MATERIAL AND METHODS: Propagated cells were divided into four groups. was given first group, andDocetaxel together third group. They exposed active substances for 24 hours. The fourth group (Control) not any substance. mRNA levels ITGA2 ITGB1 genes determined Real-time PCR method. Caspase-3 Beclin-1 staining performed immunocytochemically evaluate death. RESULTS: There increase expressions groups administered (P<0.05). decrease significant Amygdalin+Docetaxel (P<0.001). (P<0.05) immunoreactivities observed all three compared control CONCLUSIONS: increased death more than PC cells, when together, significantly reduced. Our results suggest dual treatment may prevent metastases.

Language: Английский

Amygdalin: A Review on Its Characteristics, Antioxidant Potential, Gastrointestinal Microbiota Intervention, Anticancer Therapeutic and Mechanisms, Toxicity, and Encapsulation DOI Creative Commons
Hassan Barakat, Thamer Aljutaily, Mona S. Almujaydil

et al.

Biomolecules, Journal Year: 2022, Volume and Issue: 12(10), P. 1514 - 1514

Published: Oct. 19, 2022

Bioactive amygdalin, found in high concentrations bitter almonds, has been recognized as a symbol of the cyanogenic glycoside chemical organic substance, which was initially developed pharmaceutical for treating cancer after being hydrolyzed to hydrogen cyanide (HCN). Regrettably, research shown that HCN can also damage normal cells, rendering it non-toxic human body. Extreme controversy surrounds both vivo and vitro studies, making its use risky. This review provides an extensive update on characteristics, antioxidant potential, gastrointestinal microbiota intervention, anticancer therapeutic, mechanisms, toxicity, encapsulation amygdalin. Antioxidant, anti-tumor, anti-fibrotic, antiatherosclerosis, anti-inflammatory, immunomodulatory, analgesic ability improve digestive reproductive systems, neurodegeneration, cardiac hypertrophy are just some benefits Studies verified HCN-produced amygdalin be harmful orally, but only at very doses. Although intravenous treatment less effective than oral method, route dose range 0.6 1 g daily. Amygdalin’s toxicity depends heavily variety bacteria tract. Unfortunately, there is currently no foolproof method determining microbial consortium providing safe dosage every patient. Amygdalin alginate-chitosan nanoparticles (ACNPs) relatively new area research. enhanced cytotoxic effect malignant ACNPs employed active drug-delivery system release this compound regulated, sustained manner without causing any harm healthy cells or tissues. In conclusion, large substance might next step therapy opened up due unverified conflicting data.

Language: Английский

Citations

44
The Emerging Role of Natural Products in Cancer Treatment DOI
Sumit Ghosh, Sanjib Das, Krishnendu Sinha

et al.

Archives of Toxicology, Journal Year: 2024, Volume and Issue: 98(8), P. 2353 - 2391

Published: May 25, 2024

Language: Английский

Citations

11
Phytochemistry and pharmacology of Armeniacae semen Amarum: A review DOI
Yumin Wei, Yanan Li, Shengguang Wang

et al.

Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 308, P. 116265 - 116265

Published: Feb. 15, 2023

Language: Английский

Citations

10
Amygdalin as a Promising Anticancer Agent: Molecular Mechanisms and Future Perspectives for the Development of New Nanoformulations for Its Delivery DOI Open Access
Maria Spanoudaki,

Sofia Stoumpou,

Sousana Κ. Papadopoulou

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(18), P. 14270 - 14270

Published: Sept. 19, 2023

Cancer rates are increasing, and cancer is one of the main causes death worldwide. Amygdalin, also known as vitamin B17 (and laetrile, a synthetic compound), cyanogenic glycoside compound that mainly found in kernels pulps fruits. This has been proposed for decades promising naturally occurring substance which may provide anticancer effects. comprehensive review critically summarizes scrutinizes available studies exploring effect amygdalin, highlighting its potential molecular mechanisms well need nontoxic formulation this substance. In-depth research was performed using most accurate scientific databases, e.g., PubMed, Cochrane, Embase, Medline, Scopus, Web Science, applying effective, characteristic, relevant keywords. There several pieces evidence to support idea amygdalin can exert effects against lung, breast, prostate, colorectal, cervical, gastrointestinal cancers. Amygdalin reported induce apoptosis cells, inhibiting cells’ proliferation slowing down tumor metastatic spread. However, only few have vivo animal models, while clinical remain even more scarce. The current cannot recommendation use nutritional supplements with due cyano-moiety exerts adverse side Preliminary data shown nanoparticles be alternative enhance simultaneously reducing seems agent disease development progression. there strong demand human explore prevention and/or treatment efficiency cancer. Moreover, could used lead by effectively recent developments drug discovery processes.

Language: Английский

Citations

9
The Potential of Plum Seed Residue: Unraveling the Effect of Processing on Phytochemical Composition and Bioactive Properties DOI Open Access
Sandra Rodríguez-Blázquez, Laura Pedrera-Cajas, Esther Gómez-Mejía

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1236 - 1236

Published: Jan. 19, 2024

Bioactive compounds extracted from plum seeds were identified and quantified, aiming to establish how the brandy manufacturing process affects properties possible cascade valorization of seed residues. Extraction with n-hexane using Soxhlet has provided oils rich in unsaturated fatty acids (92.24–92.51%), mainly oleic acid (72–75.56%), which is characterized by its heart-healthy properties. The fat extracts also contain tocopherols antioxidant anti-inflammatory All ethanol–water defatted neochlorogenic (90–368 µg·g−1), chlorogenic (36.1–117 protocatechuate (31.8–100 µg·g−1) that have an impact on bioactive such as antimicrobial antioxidant. Anti-amyloidogenic activity (25 mg·mL−1) was observed after both fermentation distillation extract, may be related high levels caffeic (64 ± 10 µg·g−1). principal component analysis showed all could potential applications food industry edible or cosmetic active ingredient anti-aging anti-stain cosmetics, among others. Furthermore, seeds, distillation, greatest applicability nutraceutical a supplement additive design packaging.

Language: Английский

Citations

3
Docking and Molecular Dynamics Simulation Revealed the Potential Inhibitory Activity of Amygdalin in Triple-Negative Breast Cancer Therapeutics Targeting the BRCT Domain of BARD1 Receptor DOI

Prarthana Chatterjee,

Rohit Karn, Isaac Arnold Emerson

et al.

Molecular Biotechnology, Journal Year: 2023, Volume and Issue: 66(4), P. 718 - 736

Published: Feb. 3, 2023

Language: Английский

Citations

8
Amygdalin and exercise training exert a synergistic effect in improving cardiac performance and ameliorating cardiac inflammation and fibrosis in a rat model of myocardial infarction DOI

Xiao Guo,

Feng-Xia Qu,

Jidong Zhang

et al.

Applied Physiology Nutrition and Metabolism, Journal Year: 2023, Volume and Issue: 49(3), P. 360 - 374

Published: Nov. 9, 2023

This study investigated the effects of amygdalin (AMY, a cyanogenic glycoside widely distributed in fruits and seeds

Language: Английский

Citations

5
Valorization of Defatted Cherry Seed Residues from Liquor Processing by Matrix Solid-Phase Dispersion Extraction: A Sustainable Strategy for Production of Phenolic-Rich Extracts with Antioxidant Potential DOI Creative Commons
Sandra Rodríguez-Blázquez,

Lorena Fernández-Ávila,

Esther Gómez-Mejía

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(12), P. 2041 - 2041

Published: Nov. 24, 2023

The integrated valorization of food chain waste is one the most promising alternatives in transition to a sustainable bioeconomy. Thus, an efficient solid-phase matrix dispersion extraction method, using experimental factorial design and response surface methodology, has been developed optimized for recovery polyphenols from defatted cherry seeds obtained after liquor manufacture subsequent fatty acid extraction, evaluating effect each processing step on composition phenolic content sweet residues. extracts before fermentation showed highest total (TPC) flavonoids (TFC) (3 ± 1 mg QE·g-1 1.37 0.08 GAE·g-1, respectively), while antioxidant capacity was seed both distillation. In addition, high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometer (HPLC-ESI-QTOF-MS) used determine profile. Dihydroxybenzoic acid, neochlorogenic caffeic quercetin were main phenolics found, showing differences concentration between stages production. results underline prospective by-products obtaining phenol-rich bioactive possible use different industrial sectors, offering feasible solution cascade agri-food waste.

Language: Английский

Citations

4
A Chromosome-Level Genome of ‘Xiaobaixing’ (Prunus armeniaca L.) Provides Clues to Its Domestication and Identification of Key bHLH Genes in Amygdalin Biosynthesis DOI Creative Commons

Ling Guo,

Fangjie Xie,

Xue Huang

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(15), P. 2756 - 2756

Published: July 25, 2023

Apricot is a widely cultivated fruit tree of the drupe family, and its sweet/bitter kernel traits are important indicators quality merchantability apricots. The sweetness/bitterness were mainly determined by amygdalin content. However, lack high-quality genomes has limited insight into traits. In this study, genome 'Xiaobaixing' was obtained using single-molecule sequencing chromosome-conformation capture techniques, with eight chromosomes 0.21 Gb in length 52.80% repetitive sequences. A total 29,157 protein-coding genes predicted contigs N50 = 3.56 Mb scaffold 26.73 Mb. Construction phylogenetic trees 15 species Rosaceae trees, differentiated 5.3 Ma as closest relative to 'Yinxiangbai'. GO functional annotation KEGG enrichment analysis identified 227 specific gene families 'Xiaobaixing', 569 expansion-gene 1316 contraction-gene families, including significant expansion phenylalanine N-monooxygenase β-glucosidase associated synthesis, contraction wild black cherry glucoside genes, positively selected cyanogenic amino acid metabolic pathway. 88 bHLH ParbHLH66 (rna-Par24659.1) found be key for identification kernels sequence encoded highly conserved Prunus mume, dulcis, persica, avium may participating regulation biosynthesis, which provides theoretical foundation molecular

Language: Английский

Citations

2
COMPARISON OF THE EFFECTS OF DOCETAXEL and AMYGDALIN TREATMENT ON CELL DEATH, INTEGRIN-α and INTEGRIN-β EXPRESSIONS IN DU145 PROSTATE CANCER CELL LINE DOI Open Access
Çiğdem Karaca, Evrim Suna Arıkan Söylemez, Esra Aslan

et al.

Kocatepe tıp dergisi., Journal Year: 2024, Volume and Issue: 25(4), P. 420 - 428

Published: Oct. 21, 2024

OBJECTIVE: Prostate cancer (PC) ranks second among cancer-related deaths in men, and most are caused by metastasis. Integrins, which cell surface receptors, play an important role It has been shown that integrin alpha2beta1 expression is effective adhesion, migration, invasion increasing binding to collagen I metastatic PCs. Docetaxel chemotherapy used PC, but it ineffective advanced stages. Amygdalin a cyanogenic glycoside commonly found fruit seeds, there conflict the literature regarding its effectiveness treatment. We aimed compare effects of treatments on DU145 prostate line integrinalfa2 (ITGA2) integrinbeta1 (ITGB1) expressions, as well their death, Caspase-3, Beclin-1. MATERIAL AND METHODS: Propagated cells were divided into four groups. was given first group, andDocetaxel together third group. They exposed active substances for 24 hours. The fourth group (Control) not any substance. mRNA levels ITGA2 ITGB1 genes determined Real-time PCR method. Caspase-3 Beclin-1 staining performed immunocytochemically evaluate death. RESULTS: There increase expressions groups administered (P<0.05). decrease significant Amygdalin+Docetaxel (P<0.001). (P<0.05) immunoreactivities observed all three compared control CONCLUSIONS: increased death more than PC cells, when together, significantly reduced. Our results suggest dual treatment may prevent metastases.

Language: Английский

Citations

0